Abstract：
:Functionalization of monoclonal antibodies (mAbs) requires chemical derivatization and/or genetic manipulation. Inherent in these methods are challenges with protein heterogeneity, stability and solubility. Such perturbations could potentially be avoided by using a high affinity, non-covalent intermediate to bridge the desired functionality to a stable mAb. Recently, we engineered a binding site for a peptide named "meditope" within the Fab of trastuzumab. Proximity of the meditope site to that of protein L suggested an opportunity to enhance the meditope's moderate affinity. Joined by a peptide linker, the meditope-protein L construct has a KD ~ 180 pM - a 7000-fold increase in affinity. The construct is highly specific to the engineered trastuzumab, as demonstrated by flow cytometry. Moreover, the fusion of a bulky GFP to this construct did not affect the association with cell surface antigens. Collectively, these data indicate this specific, high affinity construct can be developed to rapidly add new functionality to mAbs.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Avery KN,Zer C,Bzymek KP,Williams JCdoi
10.1038/srep07817subject
Has Abstractpub_date
2015-01-15 00:00:00pages
7817issn
2045-2322pii
srep07817journal_volume
5pub_type
杂志文章abstract：:Circular Dichroism data are often decomposed into their constituent spectra to quantify the secondary structure of peptides or proteins but the estimation of the secondary structure content fails when light scattering leads to spectral distortion. If peptide-induced liposome self-association occurs, subtracting contro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39450
更新日期：2016-12-22 00:00:00
abstract：:Methane concentration in caves is commonly much lower than the external atmosphere, yet the cave CH4 depletion causal mechanism is contested and dynamic links to external diurnal and seasonal temperature cycles unknown. Here, we report a continuous 3-year record of cave methane and other trace gases in Jenolan Caves, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07769-6
更新日期：2017-08-16 00:00:00
abstract：:Despite our increasing understanding of zebrafish heart development and regeneration, there is limited information about the distribution of endothelial cells (ECs) in the adult zebrafish heart. Here, we investigate and compare the distribution of cardiac ECs (cECs) in adult mouse and zebrafish ventricles. Surprisingl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02461-1
更新日期：2017-06-02 00:00:00
abstract：:Therapies using mesenchymal stem cell (MSC) seeded scaffolds may be applicable to various fields of regenerative medicine, including craniomaxillofacial surgery. Plastic compression of collagen scaffolds seeded with MSC has been shown to enhance the osteogenic differentiation of MSC as it increases the collagen fibril...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38814
更新日期：2016-12-09 00:00:00
abstract：:Transient gene expression approaches are valuable tools for rapid introduction of genes of interest and characterization of their functions in plants. Although agroinfiltration is the most effectively and routinely used method for transient expression of multiple genes in various plant species, this approach has been ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22436
更新日期：2016-02-29 00:00:00
abstract：:Microvascular endothelial cell heterogeneity and its relationship to hemodynamics remains poorly understood due to a lack of sufficient methods to examine these parameters in vivo at high resolution throughout an angiogenic network. The availability of surrogate markers for functional vascular proteins, such as green ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08982-z
更新日期：2017-08-22 00:00:00
abstract：:The neural representation of auditory duration remains unknown. Here, we used electroencephalogram (EEG) recordings to investigate neural oscillations during the maintenance of auditory duration in working memory (WM). EEG analyses indicated that the auditory duration length was not associated with changes in the thet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06078-2
更新日期：2017-07-18 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-61546-6
更新日期：2020-03-06 00:00:00
abstract：:The current molecular docking study provided the Free Energy of Binding (FEB) for the interaction (nanotoxicity) between VDAC mitochondrial channels of three species (VDAC1-Mus musculus, VDAC1-Homo sapiens, VDAC2-Danio rerio) with SWCNT-H, SWCNT-OH, SWCNT-COOH carbon nanotubes. The general results showed that the FEB ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13691-8
更新日期：2017-10-16 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16219
更新日期：2015-11-18 00:00:00
abstract：:Monocytes/macrophages have been found to be an important component of colitis. However, the key chemokine that initiates the CCR2+ monocytes migration from circulation to colitis tissue remains to be undiscovered. PC3-secreted microprotein (PSMP) is a novel chemokine whose receptor is CCR2. The physiological and patho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05255-7
更新日期：2017-07-11 00:00:00
abstract：:Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25030
更新日期：2016-04-29 00:00:00
abstract：:Since 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we developed a recombinant adenovirus...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02019-1
更新日期：2017-05-12 00:00:00
abstract：:Selective norepinephrine reuptake inhibitors (sNRIs) provide an effective class of approved antipsychotics, whose inhibitory mechanism could facilitate the discovery of privileged scaffolds with enhanced drug efficacy. However, the crystal structure of human norepinephrine transporter (hNET) has not been determined ye...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26883
更新日期：2016-05-27 00:00:00
abstract：:Chitin is a valuable peat substrate amendment by increasing lettuce growth and reducing the survival of the zoonotic pathogen Salmonella enterica on lettuce leaves. The production of chitin-catabolic enzymes (chitinases) play a crucial role and are mediated through the microbial community. A higher abundance of plant-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46106-x
更新日期：2019-07-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30599-z
更新日期：2018-08-13 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32059
更新日期：2016-09-12 00:00:00
abstract：:Rubber dams are widely used for landscaping in urban rivers and they retain large amounts of sediments. The sediments are rich in phosphorus (P) which can cause river eutrophication. Little is known about P release in rubber dams. We investigated the potential of sediment P release by isotherm experiment in an urban r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59493-3
更新日期：2020-02-17 00:00:00
abstract：:The precision and accuracy of quantifying semi-volatile organic compounds (SVOCs) in solution by GC/MS, particularly when volume errors are unpredictable or difficult to control, are improved by utilizing internal standards (IS). Not obvious though is the extent to which timing IS addition affects measurement. To illu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74688-4
更新日期：2020-10-19 00:00:00
abstract：:Neuroinflammation is involved in the pathogenesis of Parkinson's disease (PD) and other neurodegenerative disorders. We show that lack of PINK1- a mitochondrial kinase linked to recessive familial PD - leads to glia type-specific abnormalities of innate immunity. PINK1 loss enhances LPS/IFN-γ stimulated pro-inflammato...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18786-w
更新日期：2018-01-10 00:00:00
abstract：:Platelet activation is an important event involved in the pathophysiological processes of the coagulation system. Clinical evidence has shown that platelets undergo distinctive pathological processes during sepsis. Unfortunately, how platelets physiologically respond to inflammation or sepsis is not well understood. I...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23653
更新日期：2016-03-29 00:00:00
abstract：:Metallic silver nickel oxide honeycomb nanoarrays were synthesized via a surfactant-assisted hydrothermal route. The crystal structure of the Ag/NiO nanoarrays was confirmed by X-ray diffraction. X-ray photoelectron spectroscopy confirmed the valance state of the nickel, oxygen, and metallic silver. The morphological ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41446-0
更新日期：2019-03-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41906-7
更新日期：2019-03-29 00:00:00
abstract：:Tolerance to Dutch elm disease (DED) has been linked to the rapid and/or high induction of disease-responsive genes after infection with the fungus Ophiostoma novo-ulmi. Although the fungal infection by O. novo-ulmi primarily takes places in xylem vessels, it is still unclear how xylem contributes to the defense again...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07779-4
更新日期：2017-08-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17434-7
更新日期：2017-12-11 00:00:00
abstract：:We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement "molecular gates" for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38649
更新日期：2016-12-07 00:00:00
abstract：:The pine wood nematode, Bursaphelenchus xylophilus, is the causal agent of pine wilt disease that has devastated pine forests in Asia. Parasitic nematodes are known to have evolved antioxidant stress responses that defend against host plant defenses. In this study, the infestation of whitebark pine, Pinus bungean, wit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27438
更新日期：2016-06-07 00:00:00
abstract：:Identification of different isomer structures of atomic and molecular clusters has long been a challenging task in the field of cluster science. Here we present a three-dimensional (3D) assignment method, combining the energy (1D) and simulated (2D) spectra to assure the assignment of the global minimum structure. Thi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17738
更新日期：2015-12-03 00:00:00
abstract：:Cancer chemotherapies have improved prognosis in cancer patients, resulting in a large and rapidly increasing number of cancer survivors. "Onco-cardiology" or "cardio-oncology" is a new discipline for addressing the unanticipated cardiac side effects of newly developed cancer drugs. Lapatinib, a tyrosine kinase inhibi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57601-x
更新日期：2020-01-20 00:00:00
abstract：:The Glycoside hydrolase (GH) family 70 originally was established for glucansucrases of lactic acid bacteria (LAB) converting sucrose into α-glucan polymers. In recent years we have identified 3 subfamilies of GH70 enzymes (designated GtfB, GtfC and GtfD) as 4,6-α-glucanotransferases, cleaving (α1 → 4)-linkages in mal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07190-z
更新日期：2017-08-30 00:00:00
