Abstract:
:RNA interference (RNAi), a conserved RNA-mediated gene regulatory mechanism in eukaryotes, plays an important role in plant growth and development, and as an antiviral defence system in plants. As a counter-strategy, plant viruses encode RNAi suppressors to suppress the RNAi pathways and consequently down-regulate plant defence. In geminiviruses, the proteins AC2, AC4 and AV2 are known to act as RNAi suppressors. In this study, we have designed a gene silencing vector using the features of trans-acting small interfering RNA (tasiRNA), which is simple and can be used to target multiple genes at a time employing a single-step cloning procedure. This vector was used to target two RNAi suppressor proteins (AC2 and AC4) of the geminivirus, Tomato leaf curl New Delhi virus (ToLCNDV). The vector containing fragments of ToLCNDV AC2 and AC4 genes, on agro-infiltration, produced copious quantities of AC2 and AC4 specific siRNA in both tobacco and tomato plants. On challenge inoculation of the agro-infiltrated plants with ToLCNDV, most plants showed an absence of symptoms and low accumulation of viral DNA. Transgenic tobacco plants were raised using the AC2 and AC4 tasiRNA-generating constructs, and T1 plants, obtained from the primary transgenic plants, were tested for resistance separately against ToLCNDV and Tomato leaf curl Gujarat virus. Most plants showed an absence of symptoms and low accumulation of the corresponding viruses, the resistance being generally proportional to the amounts of siRNA produced against AC2 and AC4 genes. This is the first report of the use of artificial tasiRNA to generate resistance against an important plant virus.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Singh A,Taneja J,Dasgupta I,Mukherjee SKdoi
10.1111/mpp.12229subject
Has Abstractpub_date
2015-09-01 00:00:00pages
724-34issue
7eissn
1464-6722issn
1364-3703journal_volume
16pub_type
杂志文章abstract::Hormones are tuners of plant responses to biotic and abiotic stresses. They are involved in various complicated networks, through which they modulate responses to different stimuli. Four hormones primarily regulate plant defence to pathogens: salicylic acid (SA), jasmonic acid (JA), ethylene (Et) and abscisic acid (AB...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12204
更新日期:2015-06-01 00:00:00
abstract::The two-component signal transduction system PhoBR regulates the adaptation to phosphate limitation and the virulence of many animal bacterial pathogens. However, PhoBR in phytopathogens has rarely been investigated. In this study, we found that PhoBR in Xanthomonas oryzae pv. oryzae (Xoo), the pathogen of rice bacter...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12680
更新日期:2018-09-01 00:00:00
abstract::RXLR effectors encoded by Phytophthora species play a central role in pathogen-plant interactions. An understanding of the biological functions of RXLR effectors is conducive to the illumination of the pathogenic mechanisms and the development of disease control strategies. However, the virulence function of Phytophth...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12760
更新日期:2019-03-01 00:00:00
abstract::Citrus canker is a plant disease caused by Gram-negative bacteria from the genus Xanthomonas. The most virulent species is Xanthomonas citri ssp. citri (XAC), which attacks a wide range of citrus hosts. Differential proteomic analysis of the periplasm-enriched fraction was performed for XAC cells grown in pathogenicit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12507
更新日期:2018-01-01 00:00:00
abstract::SUMMARY The phytotoxin coronatine is a jasmonate mimic produced by Pseudomonas syringae pv. tomato (Pst). Coronatine acts as a virulence factor in Arabidopsis and mutants insensitive to coronatine are resistant to Pst and have higher levels of salicylic acid (SA). In this work we used the SA-deficient lines NahG and s...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00265.x
更新日期:2005-01-01 00:00:00
abstract::SUMMARY The genus Botrytis contains necrotrophic plant pathogens that have a wide host range (B. cinerea) or are specialized on a single host species, e.g. B. elliptica on lily. In this study, it was found that B. elliptica-induced cell death of lily displays hallmark features of animal programmed cell death or apopto...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00253.x
更新日期:2004-11-01 00:00:00
abstract::Efficient nutrient acquisition is critical to the fitness of plant pathogens. To address how the late blight agent Phytophthora infestans adapts to nutrients offered by its hosts, genes in glycolytic, gluconeogenic and amino acid pathways were mined from its genome and their expression in different plant tissues and a...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00570.x
更新日期:2009-11-01 00:00:00
abstract::The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was construct...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2011.00782.x
更新日期:2012-09-01 00:00:00
abstract::Plant-pathogenic oomycetes secrete effector proteins to suppress host immune responses. Resistance proteins may recognize effectors and activate immunity, which is often associated with a hypersensitive response (HR). Transient expression of effectors in plant germplasm and screening for HR has proven to be a powerful...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12751
更新日期:2019-02-01 00:00:00
abstract::Coffee (Coffea arabica L.), one of the key export and cash crops in tropical and subtropical countries, suffers severe losses from the rust fungus Hemileia vastatrix. The transcriptome of H. vastatrix was analysed during a compatible interaction with coffee to obtain an exhaustive repertoire of the genes expressed dur...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2011.00723.x
更新日期:2012-01-01 00:00:00
abstract::In addition to the important role of abscisic acid (ABA) in abiotic stress signalling, basal and high ABA levels appear to have a negative effect on disease resistance. Using the ABA-deficient sitiens tomato (Solanum lycopersicum) mutant and different application methods of exogenous ABA, we demonstrated the influence...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00437.x
更新日期:2008-01-01 00:00:00
abstract::It is hypothesized that the virulence of phytopathogenic fungi is mediated through the secretion of small effector proteins that interfere with the defence responses of the host plant. In Fusarium oxysporum, one family of effectors, the Secreted In Xylem (SIX) genes, has been identified. We sought to characterize the ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12594
更新日期:2018-05-01 00:00:00
abstract::Knowledge of the structure of a viroid is critically important to elucidate the roles played by the various RNA motifs in the steps of the viroid's life cycle. A new technique, RNA-selective 2'-hydroxyl acylation analysed by primer extension (SHAPE), has recently been shown to be fast, reliable and applicable to the s...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00703.x
更新日期:2011-09-01 00:00:00
abstract::SUMMARY The discovery of an endoplasmic reticulum (ER) retrieval motif at the C-terminus of the predicted Cf-9 resistance protein suggested that Cf-9 might function in the ER. To test whether Cf-9 could detect its cognate avirulence protein, Avr9, from the ER, variants of Avr9 with the ER retrieval motifs KDEL or HDEL...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2005.00274.x
更新日期:2005-03-01 00:00:00
abstract::The expression pattern of pathogenesis-related genes PR-1, PR-2 and PR-5, considered as markers for salicylic acid (SA)-dependent systemic acquired resistance (SAR), was examined in the roots and shoots of tomato plants pre-treated with SA and subsequently infected with root-knot nematodes (RKNs) (Meloidogyne incognit...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12085
更新日期:2014-04-01 00:00:00
abstract::Leptosphaeria maculans, the causal agent of blackleg disease, interacts with Brassica napus (oilseed rape, canola) and other Brassica hosts in a gene-for-gene manner. The avirulence gene AvrLmJ1 has been cloned previously and shown to interact with an unidentified Brassica juncea resistance gene. In this study, we sho...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12574
更新日期:2018-04-01 00:00:00
abstract::SUMMARY Two of the currently widely accepted species in the section Nigrescentia of the genus Verticillium are major plant pathogens inducing wilt diseases in a wide range of mainly dicotyledonous hosts. Three species closely related to these two are less important wilt pathogens and soil saprophytes. A sixth species,...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2003.00172.x
更新日期:2003-07-01 00:00:00
abstract::The cellular outcome of changes in nitrogen availability in the context of development and early stages of pathogenicity was studied by quantitative analysis of two-dimensional gel electrophoresis of Colletotrichum acutatum infecting strawberry. Significant alterations occurred in the abundance of proteins synthesized...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00454.x
更新日期:2008-03-01 00:00:00
abstract::The type III secretion system (T3SS) is required for Xanthomonas citri subsp. citri (Xcc) virulence by translocating effectors into host cytoplasm to promote disease development. The T3SS is controlled by the master transcriptional regulators HrpG and HrpX. While the function of HrpG and HrpX are well characterized, t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12786
更新日期:2019-05-01 00:00:00
abstract::The single-stranded, positive-sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Fou...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12366
更新日期:2016-09-01 00:00:00
abstract::A 3'-terminal, 77-nucleotide sequence of Bamboo mosaic virus (BaMV) minus-strand RNA (Ba-77), comprising a 5' stem-loop, a spacer and a 3'-CUUUU sequence, can be used to initiate plus-strand RNA synthesis in vitro. To understand the mechanism of plus-strand RNA synthesis, mutations were introduced in the 5' untranslat...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2009.00597.x
更新日期:2010-03-01 00:00:00
abstract::The use of genetic resistance is considered to be the most effective and sustainable approach to the control of plant pathogens. Although most of the known natural resistance genes are monogenic dominant R genes that are predominant against fungi and bacteria, more and more recessive resistance genes against viruses h...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/j.1364-3703.2012.00791.x
更新日期:2012-09-01 00:00:00
abstract::Abstract To identify virulence genes of P. syringae pv. tomato strain DC3000 we screened for mutants with reduced virulence on its plant hosts, Arabidopsis thaliana and tomato. We isolated a Tn5-insertion mutant that exhibited reduced virulence on both hosts. Further characterization showed that this mutant carried a ...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1046/j.1364-3703.2000.00016.x
更新日期:2000-03-01 00:00:00
abstract::Fungal plant pathogens secrete effector proteins and metabolites to cause disease. Additionally, some species transfer small RNAs (sRNAs) into plant cells to silence host mRNAs through complementary base pairing and suppress plant immunity. The fungus Sclerotinia sclerotiorum infects over 600 plant species, but little...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12841
更新日期:2019-09-01 00:00:00
abstract::SUMMARY The cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. Plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. Expres...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2007.00394.x
更新日期:2007-05-01 00:00:00
abstract::LysR-type transcriptional regulators (LTTRs) are ubiquitous and abundant amongst bacteria and control a variety of cellular processes. Here, we investigated the effect of Rsc1880 (a putative LTTR, hereafter designated as PrhO) on the pathogenicity of Ralstonia solanacearum. Deletion of prhO substantially reduced the e...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12660
更新日期:2018-01-24 00:00:00
abstract::SUMMARY We describe the use of degenerate primers to amplify the core hrp/hrc region of P. syringae pv. phaseolicola NPS3121. 18 826 bp were amplified using long-range PCR, digested and shotgun sequenced. Sequence analysis finds a hypervariable region between the hrpU and hrpC operons, and indications of positive sele...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2004.00212.x
更新日期:2004-03-01 00:00:00
abstract::Histone acetylation has been established as a principal epigenetic regulatory mechanism in eukaryotes. Sas3, a histone acetyltransferase belonging to the largest family of acetyltransferase, MYST, is the catalytic subunit of a conserved histone acetyltransferase complex. To date, the functions of Sas3 and its ortholog...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12856
更新日期:2019-11-01 00:00:00
abstract::SUMMARY Fusarium graminearum is the main causative agent of Fusarium head blight on small grain cereals and of ear rot on maize. The disease leads to dramatic yield losses and to an accumulation of mycotoxins. The most dominant F. graminearum mycotoxins are the trichothecenes, with deoxynivalenol and nivalenol being t...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/j.1364-3703.2006.00351.x
更新日期:2006-11-01 00:00:00
abstract::Crop diseases cause significant yield losses, and the use of resistant cultivars can effectively mitigate these losses and control many plant diseases. Most plant resistance (R) genes encode immune receptors composed of nucleotide-binding and leucine-rich repeat (NLR) domains. These proteins mediate the specific recog...
journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
doi:10.1111/mpp.12723
更新日期:2018-11-01 00:00:00