Abstract:
:The cellular outcome of changes in nitrogen availability in the context of development and early stages of pathogenicity was studied by quantitative analysis of two-dimensional gel electrophoresis of Colletotrichum acutatum infecting strawberry. Significant alterations occurred in the abundance of proteins synthesized during appressorium formation under nitrogen-limiting conditions compared with a complete nutrient supply. Proteins that were up- or down-regulated were involved in energy metabolism, nitrogen and amino acid metabolism, protein synthesis and degradation, response to stress and reactive oxygen scavenging. Members belonging to the reactive oxygen species (ROS) scavenger machinery, superoxide dismutase and glutathione peroxidase, were up-regulated at the appressorium formation stage, as well as under nitrogen-limiting conditions relative to growth with a complete nutrient supply, whereas abundance of bifunctional catalase was up-regulated predominantly at the appressorium formation stage. Fungal ROS were detected within germinating conidia during host pre-penetration, penetration and colonization stages, accompanied by plant ROS, which were abundant in the apoplastic space. Application of exogenous antioxidants quenched ROS production and reduced the frequency of appressorium formation. Up-regulation in metabolic activity was detected during appressorium formation and nutrient deficiency compared with growth under complete nutrient supply. Enhanced levels of proteins related to the glyoxylate cycle and lipid metabolism (malate dehydrogenase, formate dehydrogenase and acetyl-CoA acetyltransferase) were observed at the appressorium formation stage, in contrast to down-regulation of isocitrate dehydrogenase. The present study demonstrates that appressoria formation processes, occurring under nutritional deprivation, are accompanied by metabolic shifts, and that ROS production is an early fungal response that may modulate initial stages of pathogen development.
journal_name
Mol Plant Patholjournal_title
Molecular plant pathologyauthors
Brown SH,Yarden O,Gollop N,Chen S,Zveibil A,Belausov E,Freeman Sdoi
10.1111/j.1364-3703.2007.00454.xsubject
Has Abstractpub_date
2008-03-01 00:00:00pages
171-90issue
2eissn
1464-6722issn
1364-3703pii
MPP454journal_volume
9pub_type
杂志文章abstract::SUMMARY It is becoming increasingly evident that a plant-pathogen interaction may be compared to an open warfare, whose major weapons are proteins synthesized by both organisms. These weapons were gradually developed in what must have been a multimillion-year evolutionary game of ping-pong. The outcome of each battle ...
journal_title:Molecular plant pathology
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更新日期:2007-09-01 00:00:00
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更新日期:2003-11-01 00:00:00
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更新日期:2010-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-11-01 00:00:00
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更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1364-3703.2010.00703.x
更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1464-6722.2001.00068.x
更新日期:2001-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-09-01 00:00:00
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更新日期:2012-10-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章,评审
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更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mpp.12366
更新日期:2016-09-01 00:00:00
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journal_title:Molecular plant pathology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
abstract::Fusarium oxysporum f. sp. radicis-cucumerinum (Forc) causes severe root rot and wilt in several cucurbit species, including cucumber, melon, and watermelon. Previously, a pathogenicity chromosome, chrRC , was identified in Forc. Strains that were previously nonpathogenic could infect multiple cucurbit species after ob...
journal_title:Molecular plant pathology
pub_type: 杂志文章
doi:10.1111/mpp.12927
更新日期:2020-06-01 00:00:00