Abscisic acid prevents the coalescence of protein storage vacuoles by upregulating expression of a tonoplast intrinsic protein gene in barley aleurone.


:Tonoplast intrinsic proteins (TIPs) are integral membrane proteins that are known to function in plants as aquaporins. Here, we propose another role for TIPs during the fusion of protein storage vacuoles (PSVs) in aleurone cells, a process that is promoted by gibberellic acid (GA) and prevented by abscisic acid (ABA). Studies of the expression of barley (Hordeum vulgare) TIP genes (HvTIP) showed that GA specifically decreased the abundance of HvTIP1;2 and HvTIP3;1 transcripts, while ABA strongly increased expression of HvTIP3;1. Increased or decreased expression of HvTIP3;1 interfered with the hormonal effects on vacuolation in aleurone protoplasts. HvTIP3;1 gain-of-function experiments delayed GA-induced vacuolation, whereas HvTIP3;1 loss-of-function experiments promoted vacuolation in ABA-treated aleurone cells. These results indicate that TIP plays a key role in preventing the coalescence of small PSVs in aleurone cells. Hormonal regulation of the HvTIP3;1 promoter is similar to the regulation of the endogenous gene, indicating that induction of the transcription of HvTIP3;1 by ABA is a critical factor in the prevention of PSV coalescence in response to ABA. Promoter analysis using deletions and site-directed mutagenesis of sequences identified three cis-acting elements that are responsible for ABA responsiveness in the HvTIP3;1 promoter. Promoter analysis also showed that ABA responsiveness of the HvTIP3;1 promoter is likely to occur via a unique regulatory system distinct from that involving the ABA-response promoter complexes.


J Exp Bot


Lee SE,Yim HK,Lim MN,Yoon Is,Kim Jh,Hwang YS




Has Abstract


2015-03-01 00:00:00














  • The (r)evolution of gene regulatory networks controlling Arabidopsis plant reproduction: a two-decade history.

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    journal_title:Journal of experimental botany

    pub_type: 信件,评审


    authors: Pajoro A,Biewers S,Dougali E,Leal Valentim F,Mendes MA,Porri A,Coupland G,Van de Peer Y,van Dijk AD,Colombo L,Davies B,Angenent GC

    更新日期:2014-09-01 00:00:00

  • Use of mutants to study long-distance signalling in response to compacted soil.

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    authors: Roberts JA,Hussain A,Taylor IB,Black CR

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    authors: Trenner J,Poeschl Y,Grau J,Gogol-Döring A,Quint M,Delker C

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    journal_title:Journal of experimental botany

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    journal_title:Journal of experimental botany

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    authors: Sowiński P,Szczepanik J,Minchin PE

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  • Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize.

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    authors: Cordoba E,Porta H,Arroyo A,San Román C,Medina L,Rodríguez-Concepción M,León P

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    authors: Ioannidi E,Kalamaki MS,Engineer C,Pateraki I,Alexandrou D,Mellidou I,Giovannonni J,Kanellis AK

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    authors: Frébort I,Kowalska M,Hluska T,Frébortová J,Galuszka P

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    authors: Oliva M,Butenko Y,Hsieh TF,Hakim O,Katz A,Smorodinsky NI,Michaeli D,Fischer RL,Ohad N

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    journal_title:Journal of experimental botany

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    journal_title:Journal of experimental botany

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    authors: Lian H,Li X,Liu Z,He Y

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cota-Sánchez JH,Abreu DD

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    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Gajdosová S,Spíchal L,Kamínek M,Hoyerová K,Novák O,Dobrev PI,Galuszka P,Klíma P,Gaudinová A,Zizková E,Hanus J,Dancák M,Trávnícek B,Pesek B,Krupicka M,Vanková R,Strnad M,Motyka V

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    journal_title:Journal of experimental botany

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    authors: Ashida H,Saito Y,Nakano T,Tandeau de Marsac N,Sekowska A,Danchin A,Yokota A

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    更新日期:2016-04-01 00:00:00