Abstract:
:DNA sequencing was dominated by Sanger's chain termination method until the mid-2000s, when it was progressively supplanted by new sequencing technologies that can generate much larger quantities of data in a shorter time. At the forefront of these developments, long-read sequencing technologies (third-generation sequencing) can produce reads that are several kilobases in length. This greatly improves the accuracy of genome assemblies by spanning the highly repetitive segments that cause difficulty for second-generation short-read technologies. Third-generation sequencing is especially appealing for plant genomes, which can be extremely large with long stretches of highly repetitive DNA. Until recently, the low basecalling accuracy of third-generation technologies meant that accurate genome assembly required expensive, high-coverage sequencing followed by computational analysis to correct for errors. However, today's long-read technologies are more accurate and less expensive, making them the method of choice for the assembly of complex genomes. Oxford Nanopore Technologies (ONT), a third-generation platform for the sequencing of native DNA strands, is particularly suitable for the generation of high-quality assemblies of highly repetitive plant genomes. Here we discuss the benefits of ONT, especially for the plant science community, and describe the issues that remain to be addressed when using ONT for plant genome sequencing.
journal_name
J Exp Botjournal_title
Journal of experimental botanyauthors
Dumschott K,Schmidt MH,Chawla HS,Snowdon R,Usadel Bdoi
10.1093/jxb/eraa263subject
Has Abstractpub_date
2020-09-19 00:00:00pages
5313-5322issue
18eissn
0022-0957issn
1460-2431pii
5847836journal_volume
71pub_type
杂志文章abstract::It has been suggested that antioxidants play a role in regulating or modulating senescence dynamics of plant tissues. Ethylene has been shown to promote early plant senescence while controlled atmospheres (CA; reduced O2 levels and elevated CO2 levels) can delay its onset and/or severity. In order to examine the possi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/51.344.645
更新日期:2000-03-01 00:00:00
abstract::During leaf senescence and fruit ripening, chlorophyll is degraded in a multistep pathway into linear tetrapyrroles called phyllobilins. A key feature of chlorophyll breakdown is the removal of the hydrophobic phytol chain that renders phyllobilins water soluble, an important prerequisite for their ultimate storage in...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx326
更新日期:2018-02-12 00:00:00
abstract::Understanding and diagnosing plant responses to stress will benefit greatly from three-dimensional (3D) measurement and analysis of plant properties because plant responses are strongly related to their 3D structures. Light detection and ranging (lidar) has recently emerged as a powerful tool for direct 3D measurement...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erl142
更新日期:2007-01-01 00:00:00
abstract::High temperatures are known to reduce anthocyanin accumulation in a number of diverse plant species. In potato (Solanum tuberosum L.), high temperature significantly reduces tuber anthocyanin pigment content. However, the mechanism of anthocyanin biosynthesis in potato tuber under heat stress remains unknown. Here we ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erz194
更新日期:2019-08-07 00:00:00
abstract::The activity of ionically bound peroxidases from an asparagus spear increased from 5-24 h post-harvest. Isoelectric focusing showed that the post-harvest increase of the total peroxidase activity was due to the increase of several distinct isoperoxidases. Concomitantly, a decrease in the activity of two anionic peroxi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erg253
更新日期:2003-10-01 00:00:00
abstract::In recent years, efforts to exploit sunlight, a free and abundant energy source, have sped up dramatically. Oxygenic photosynthetic organisms, such as higher plants, algae, and cyanobacteria, can convert solar energy into chemical energy very efficiently using water as an electron donor. By providing organic building ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erz054
更新日期:2019-03-27 00:00:00
abstract::The aerial portion of a plant, namely the leaf, is inhabited by pathogenic and non-pathogenic microbes. The leaf's physical and chemical properties, combined with fluctuating and often challenging environmental factors, create surfaces that require a high degree of adaptation for microbial colonization. As a consequen...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa417
更新日期:2021-01-20 00:00:00
abstract::Sulfate transporters are integral membrane proteins controlling the flux of sulfate (SO42-) entering the cells and subcellular compartments across the membrane lipid bilayers. Sulfate uptake is a dynamic biological process that occurs in multiple cell layers and organs in plants. In vascular plants, sulfate ions are t...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erz132
更新日期:2019-08-19 00:00:00
abstract::Tonoplast intrinsic proteins (TIPs) are integral membrane proteins that are known to function in plants as aquaporins. Here, we propose another role for TIPs during the fusion of protein storage vacuoles (PSVs) in aleurone cells, a process that is promoted by gibberellic acid (GA) and prevented by abscisic acid (ABA)....
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru467
更新日期:2015-03-01 00:00:00
abstract::Zinc (Zn), an essential nutrient in cells, plays a vital role in controlling cellular processes such as growth, development, and differentiation. Although the mechanisms of Zn translocation in rice plants (Oryza sativa) are not fully understood, it has recently received increased interest. OsZIP4 is a Zn transporter t...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm147
更新日期:2007-01-01 00:00:00
abstract::Protein kinases and phosphatases are the foremost agents which take part in cell cycle regulation in both plants and other eukaryotes. Protein kinases are a very well examined group of proteins with respect to chemical structure and function. Nowadays protein phosphatases, including PP1 and PP2A belonging to the PSP f...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erm148
更新日期:2007-01-01 00:00:00
abstract::Potato has been genetically engineered for the production of commercially important ketocarotenoids including astaxanthin (3,3'-dihydroxy 4,4'-diketo-beta-carotene). To support the formation of 3-hydroxylated and 4-ketolated beta-carotene, a transgenic potato line accumulating zeaxanthin due to inactivated zeaxanthin ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erl103
更新日期:2006-01-01 00:00:00
abstract::Leaf senescence is a programmed developmental process governed by various endogenous and exogenous factors, such as the plant developmental stage, leaf age, phytohormone levels, darkness, and exposure to stresses. It was found that, in addition to its well-documented role in the enhancement of plant frost tolerance, o...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erp300
更新日期:2010-01-01 00:00:00
abstract::ICE1 (Inducer of CBF Expression 1) encodes a MYC-like basic helix-loop-helix transcription factor that acts as a central regulator of cold response. In this study, we elucidated the function and underlying mechanisms of PtrICE1 from trifoliate orange [Poncirus trifoliata (L.) Raf.]. PtrICE1 was upregulated by cold, de...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erv138
更新日期:2015-06-01 00:00:00
abstract::Global change factors affect plant carbon uptake in concert. In order to investigate the response directions and potential interactive effects, and to understand the underlying mechanisms, multifactor experiments are needed. The focus of this study was on the photosynthetic response to elevated CO(2) [CO2; free air CO...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/err133
更新日期:2011-08-01 00:00:00
abstract::One strategy deployed by plants to endure water scarcity is to accelerate the transition to flowering adaptively via the drought escape (DE) response. In Arabidopsis thaliana, activation of the DE response requires the photoperiodic response gene GIGANTEA (GI) and the florigen genes FLOWERING LOCUS T (FT) and TWIN SIS...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erw384
更新日期:2016-12-01 00:00:00
abstract::Ozone induces rapid activation of SIPK, a mitogen-activated protein kinase (MAPK) in tobacco. Through transgenic manipulation it has previously been shown that overexpression of SIPK leads to enhanced ozone-induced lesion formation with concomitant accumulation of ROS. In spite of this hypersensitive phenotype, the ef...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eri219
更新日期:2005-08-01 00:00:00
abstract::Photoreceptors perceive different wavelengths of light and transduce light signals downstream via a range of proteins. COP1, an E3 ubiquitin ligase, regulates light signaling by mediating the ubiquitination and subsequent proteasomal degradation of photoreceptors such as phytochromes and cryptochromes, as well as vari...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx312
更新日期:2017-10-13 00:00:00
abstract::Regulated cell death plays key roles during essential processes along the plant life cycle. It takes part of specific developmental programs and maintains the organism homeostasis in response to unfavorable environments. Ferroptosis is a recently discovered iron-dependent cell death pathway characterized by the accumu...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eraa425
更新日期:2020-09-11 00:00:00
abstract::Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-carotene is generated from lycopene by the lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 ...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erab029
更新日期:2021-01-23 00:00:00
abstract::Mosses are among the earliest branching embryophytes and probably originated not later than the early Ordovician when atmospheric CO2 was higher and O2 was lower than today. The C3 biochemistry and physiology of their photosynthesis suggests, by analogy with tracheophytes, that growth of extant bryophytes in high CO2 ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erw012
更新日期:2016-03-01 00:00:00
abstract::Storage of phosphorus (P) in stem tissue is important in Mediterranean Proteaceae, because proteoid root growth and P uptake is greatest during winter, whereas shoot growth occurs mostly in summer. This has prompted the present investigation of the P distribution amongst roots, stems, and leaves of Hakea prostrata R.B...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erh111
更新日期:2004-05-01 00:00:00
abstract::The relationship between photosynthetic energy conservation and thermal dissipation of light energy is considered, with emphasis on organisms which tolerate full desiccation without suffering photo-oxidative damage in strong light. As soon as water becomes available to dry poikilohydric organisms, they resume photosyn...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erj104
更新日期:2006-01-01 00:00:00
abstract::Rapid alkalinization factor (RALF) is a peptide signal that plays a basic role in cell biology and most likely regulates cell expansion. In this study, transgenic Arabidopsis thaliana lines with high and low levels of AtRALF1 transcripts were used to investigate this peptide's mechanism of action. Overexpression of th...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/eru099
更新日期:2014-05-01 00:00:00
abstract::MicroRNAs (miRNAs) are known to regulate expression of genes under stress. We report here the deep sequencing of small RNAs expressed during control, short and prolonged heat stress and recovery. Genome-wide identification of miRNAs in tolerant (Nagina 22) and susceptible (Vandana) rice cultivars was performed in 16 s...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erx111
更新日期:2017-04-01 00:00:00
abstract::AGAMOUS (AG) MADS-box transcription factors have been shown to play crucial roles in floral organ and fruit development in angiosperms. Here, we isolated a tomato (Solanum lycopersicum) AG MADS-box gene SlMBP3 and found that it is preferentially expressed in flowers and during early fruit developmental stages in the w...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery418
更新日期:2019-02-05 00:00:00
abstract::Transgenic tobacco (Nicotiana tabacum cv. LA Burley 21) lines expressing three genes encoding enzymes thought to be critical for the efficient production of phytochelatins, (i) serine acetyltransferase (EC 2.3.1.30) involved in the production of O-acetylserine, the cysteine precursor, (ii) gamma-glutamylcysteine synth...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/erj176
更新日期:2006-01-01 00:00:00
abstract::Capsella is a small genus within the mustard family (Brassicaceae). Its three species, however, show many evolutionary trends also observed in other Brassicaceae (including Arabidopsis) and far beyond, including transitions from a diploid, self-incompatible, obligatory outcrossing species with comparatively large and ...
journal_title:Journal of experimental botany
pub_type: 杂志文章,评审
doi:10.1093/jxb/erl158
更新日期:2006-01-01 00:00:00
abstract::In recent years, post-translational modification (PTM) of proteins has emerged as a key process that integrates plant growth and response to a changing environment. During the processes of domestication and breeding, plants were selected for various yield and adaptational characteristics. The post-translational modifi...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jxb/ery222
更新日期:2018-08-31 00:00:00
abstract::Water is a principal limitation to agricultural production during drought and in arid regions of the world. Mechanisms that plants use to cope with drought can be grouped into two different strategies: drought tolerance and drought avoidance. Previous efforts toward engineering plants for improved performance during d...
journal_title:Journal of experimental botany
pub_type: 杂志文章
doi:10.1093/jexbot/53.369.699
更新日期:2002-04-01 00:00:00