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Okadaic acid (1 microM) accelerates S phase and mitosis but inhibits heterochromatin replication and metaphase anaphase transition in Vicia faba meristem cells.

Abstract：

:Protein kinases and phosphatases are the foremost agents which take part in cell cycle regulation in both plants and other eukaryotes. Protein kinases are a very well examined group of proteins with respect to chemical structure and function. Nowadays protein phosphatases, including PP1 and PP2A belonging to the PSP family, are the focus of interest. Okadaic acid (OA) which is a specific inhibitor of protein phosphatase activity is widely used to study them. In the present research, the involvement of OA-sensitive phosphatases in the regulation of progression of the plant cell cycle was analysed (in planta) using Vicia faba root meristems synchronized with hydroxyurea and divided into five series. Each series was treated with 1 muM OA for 3 h for different time periods corresponding to the consecutive cell cycle phases. The results showed that in the OA-treated cells DNA replication and mitosis began earlier than in the control cells, since G(1) and G(2) phases were significantly shorter and the H1 histone kinases activity was higher. Moreover, autoradiography and morphological analyses of mitotic figures revealed that the OA-treated cells entered mitosis before the end of heterochromatin replication. An immunocytochemical search showed that earlier initiation of S phase in the OA-treated cells correlated with more abundant phosphorylation of Rb-like protein in comparison with the control cells. OA also induced significant condensation of metaphase chromosomes and blocked metaphase-anaphase transition.

journal_name

J Exp Bot

journal_title

Journal of experimental botany

authors

Polit JT,Kazmierczak A

doi

10.1093/jxb/erm148

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

2785-97

issue

11

eissn

0022-0957

issn

1460-2431

pii

erm148

journal_volume

58

pub_type

杂志文章

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