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Ketocarotenoid formation in transgenic potato.

Abstract：

:Potato has been genetically engineered for the production of commercially important ketocarotenoids including astaxanthin (3,3'-dihydroxy 4,4'-diketo-beta-carotene). To support the formation of 3-hydroxylated and 4-ketolated beta-carotene, a transgenic potato line accumulating zeaxanthin due to inactivated zeaxanthin epoxidase was co-transformed with the crtO beta-carotene ketolase gene from the cyanobacterium Synechocystis under a constitutive promoter. Plants were generated which exhibited expression of this gene, resulting in an accumulation of echinenone, 3'-hydroxyechinenone, and 4-ketozeaxanthin in leaves, as well as 3'-hydroxyechinenone, 4-ketozeaxanthin together with astaxanthin in the tuber. The amount of ketocarotenoids formed represent approximately 10-12% of total carotenoids in leaves and tubers. Negative effects on photosynthesis due to the presence of the ketocarotenoids in leaves could be excluded by the determination of variable fluorescence.

journal_name

J Exp Bot

journal_title

Journal of experimental botany

authors

Gerjets T,Sandmann G

doi

10.1093/jxb/erl103

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

3639-45

issue

14

eissn

0022-0957

issn

1460-2431

pii

erl103

journal_volume

57

pub_type

杂志文章

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