当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Range of cell-wall alterations enhance saccharification in Brachypodium distachyon mutants.

Range of cell-wall alterations enhance saccharification in Brachypodium distachyon mutants.

Abstract：

:Lignocellulosic plant biomass is an attractive feedstock for the production of sustainable biofuels, but the commercialization of such products is hampered by the high costs of processing this material into fermentable sugars (saccharification). One approach to lowering these costs is to produce crops with cell walls that are more susceptible to hydrolysis to reduce preprocessing and enzyme inputs. To deepen our understanding of the molecular genetic basis of lignocellulose recalcitrance, we have screened a mutagenized population of the model grass Brachypodium distachyon for improved saccharification with an industrial polysaccharide-degrading enzyme mixture. From an initial screen of 2,400 M2 plants, we selected 12 lines that showed heritable improvements in saccharification, mostly with no significant reduction in plant size or stem strength. Characterization of these putative mutants revealed a variety of alterations in cell-wall components. We have mapped the underlying genetic lesions responsible for increased saccharification using a deep sequencing approach, and here we report the mapping of one of the causal mutations to a narrow region in chromosome 2. The most likely candidate gene in this region encodes a GT61 glycosyltransferase, which has been implicated in arabinoxylan substitution. Our work shows that forward genetic screening provides a powerful route to identify factors that impact on lignocellulose digestibility, with implications for improving feedstock for cellulosic biofuel production.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Marriott PE,Sibout R,Lapierre C,Fangel JU,Willats WG,Hofte H,Gómez LD,McQueen-Mason SJ

doi

10.1073/pnas.1414020111

subject

Has Abstract

pub_date

2014-10-07 00:00:00

pages

14601-6

issue

40

eissn

0027-8424

issn

1091-6490

pii

1414020111

journal_volume

111

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid.

    abstract：:Fungal cell walls and fragments thereof (elicitors) induce the formation of low and high molecular weight defense compounds in plant cell suspension cultures. This induced synthesis requires a signal molecule transmitting the message between the elicitor plant cell wall receptor and gene activation. We demonstrate in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.16.7490

    authors： Mueller MJ,Brodschelm W,Spannagl E,Zenk MH

    更新日期：1993-08-15 00:00:00

  • Therapeutic antitumor immunity by checkpoint blockade is enhanced by ibrutinib, an inhibitor of both BTK and ITK.

    abstract：:Monoclonal antibodies can block cellular interactions that negatively regulate T-cell immune responses, such as CD80/CTLA-4 and PD-1/PD1-L, amplifying preexisting immunity and thereby evoking antitumor immune responses. Ibrutinib, an approved therapy for B-cell malignancies, is a covalent inhibitor of BTK, a member of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1500712112

    authors： Sagiv-Barfi I,Kohrt HE,Czerwinski DK,Ng PP,Chang BY,Levy R

    更新日期：2015-03-03 00:00:00

  • Signaling via beta1 integrins and mitogen-activated protein kinase determines human epidermal stem cell fate in vitro.

    abstract：:Human epidermal stem cells express higher levels of beta1 integrins and are more adhesive than keratinocytes that are destined to differentiate. To investigate whether high beta1 integrin expression and adhesiveness are essential for maintaining keratinocytes in the stem cell compartment, we introduced a dominant-nega...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.12.6728

    authors： Zhu AJ,Haase I,Watt FM

    更新日期：1999-06-08 00:00:00

  • Dialyzable factor in human serum of platelet origin stimulates endothelial cell replication and growth.

    abstract：:Porcine aortic endothelial cells were isolated and maintained in Dulbecco's modified Eagle's medium (DME medium)/10% citrate-treated human plasma. They were stimulated by DME medium/10% human serum to grow from a density of 10,100 +/- 500 per well to a final density of 83,000 +/- 1,800 per well over a 9-day period. On...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.6.1641

    authors： Clemmons DR,Isley WL,Brown MT

    更新日期：1983-03-01 00:00:00

  • Alteration of epidermal growth factor-dependent phosphorylation during rat liver regeneration.

    abstract：:Epidermal growth factor (EGF) stimulates membrane protein phosphorylation in a human cell line, A-431. The known hepatic mitogenic action of EGF and the reduction in EGF receptor number that occurs during liver regeneration led us to study whether EGF-dependent protein kinase activity was present in rat liver and whet...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.3.776

    authors： Rubin RA,O'Keefe EJ,Earp HS

    更新日期：1982-02-01 00:00:00

  • Glucose-regulated interaction of a regulatory subunit of protein phosphatase 1 with the Snf1 protein kinase in Saccharomyces cerevisiae.

    abstract：:The Snf1 protein kinase family has been conserved in eukaryotes. In the yeast Saccharomyces cerevisiae, Snf1 is essential for transcription of glucose-repressed genes in response to glucose starvation. The direct interaction between Snf1 and its activating subunit, Snf4, within the kinase complex is regulated by the g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6245

    authors： Ludin K,Jiang R,Carlson M

    更新日期：1998-05-26 00:00:00

  • BDNF pro-peptide actions facilitate hippocampal LTD and are altered by the common BDNF polymorphism Val66Met.

    abstract：:Most growth factors are initially synthesized as precursor proteins and subsequently processed into their mature form by proteolytic cleavage, resulting in simultaneous removal of a pro-peptide. However, compared with that of mature form, the biological role of the pro-peptide is poorly understood. Here, we investigat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1422336112

    authors： Mizui T,Ishikawa Y,Kumanogoh H,Lume M,Matsumoto T,Hara T,Yamawaki S,Takahashi M,Shiosaka S,Itami C,Uegaki K,Saarma M,Kojima M

    更新日期：2015-06-09 00:00:00

  • Identification of divergent type VI secretion effectors using a conserved chaperone domain.

    abstract：:The type VI secretion system (T6SS) is a lethal weapon used by many bacteria to kill eukaryotic predators or prokaryotic competitors. Killing by the T6SS results from repetitive delivery of toxic effectors. Despite their importance in dictating bacterial fitness, systematic prediction of T6SS effectors remains challen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1505317112

    authors： Liang X,Moore R,Wilton M,Wong MJ,Lam L,Dong TG

    更新日期：2015-07-21 00:00:00

  • Presenilin mutations linked to familial Alzheimer's disease cause an imbalance in phosphatidylinositol 4,5-bisphosphate metabolism.

    abstract：:Phosphatidylinositol 4,5-bisphosphate (PIP2) is an important cellular effector whose functions include the regulation of ion channels and membrane trafficking. Aberrant PIP2 metabolism has also been implicated in a variety of human disease states, e.g., cancer and diabetes. Here we report that familial Alzheimer's dis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0604954103

    authors： Landman N,Jeong SY,Shin SY,Voronov SV,Serban G,Kang MS,Park MK,Di Paolo G,Chung S,Kim TW

    更新日期：2006-12-19 00:00:00

  • Strain-specific innate immune signaling pathways determine malaria parasitemia dynamics and host mortality.

    abstract：:Malaria infection triggers vigorous host immune responses; however, the parasite ligands, host receptors, and the signaling pathways responsible for these reactions remain unknown or controversial. Malaria parasites primarily reside within RBCs, thereby hiding themselves from direct contact and recognition by host imm...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1316467111

    authors： Wu J,Tian L,Yu X,Pattaradilokrat S,Li J,Wang M,Yu W,Qi Y,Zeituni AE,Nair SC,Crampton SP,Orandle MS,Bolland SM,Qi CF,Long CA,Myers TG,Coligan JE,Wang R,Su XZ

    更新日期：2014-01-28 00:00:00

  • Transfection of Fv-1 permissive and restrictive mouse cells with integrated DNA of murine leukemia viruses.

    abstract：:Whole-cell DNA preparations isolated from SC-1 cells chronically infected with N- or B-tropic murine leukemia viruses (MuLV) were tested for infectious activity in an Fv-1n (NIH-3T3) and two Fv-Ib (C57BL/6 and SV-A31) cell cultures. Efficiency of transfection for all DNAs was better in the NIH-3T3 cells than in C57BL/...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.3.1451

    authors： Hsu IC,Yang WK,Tennant RW,Brown A

    更新日期：1978-03-01 00:00:00

  • Quality control of ribosomal RNA mediated by polynucleotide phosphorylase and RNase R.

    abstract：:Despite their overall accuracy, errors in macromolecular processes, such as rRNA synthesis and ribosome assembly, inevitably occur. However, whether these errors are remediated and how this might be accomplished is not known. In previous work, we showed that a double mutant strain lacking both polynucleotide phosphory...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1231041100

    authors： Cheng ZF,Deutscher MP

    更新日期：2003-05-27 00:00:00

  • Constituents and functional implications of the rat default mode network.

    abstract：:The default mode network (DMN) has been suggested to support a variety of self-referential functions in humans and has been fractionated into subsystems based on distinct responses to cognitive tasks and functional connectivity architecture. Such subsystems are thought to reflect functional hierarchy and segregation w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1601485113

    authors： Hsu LM,Liang X,Gu H,Brynildsen JK,Stark JA,Ash JA,Lin CP,Lu H,Rapp PR,Stein EA,Yang Y

    更新日期：2016-08-02 00:00:00

  • Purified inositol hexakisphosphate kinase is an ATP synthase: diphosphoinositol pentakisphosphate as a high-energy phosphate donor.

    abstract：:Diphosphoinositol pentakisphosphate (PP-IP5) and bis(diphospho)inositol tetrakisphosphate (bis-PP-IP4) are recently identified inositol phosphates that possess pyrophosphate bonds. We have purified an inositol hexakisphosphate (IP6) kinase from rat brain supernatants. The pure protein, a monomer of 54 kDa, displays hi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.9.4305

    authors： Voglmaier SM,Bembenek ME,Kaplin AI,Dormán G,Olszewski JD,Prestwich GD,Snyder SH

    更新日期：1996-04-30 00:00:00

  • An electrophoretic variant of beta-galactosidase with altered catalytic properties in a patient with GM1 gangliosidosis.

    abstract：:In nine patients with GM1 gangliosidosis, liver ganglioside GM1 beta-galactosidase (EC 3.2.1.23) activity ranged from less than 0.01% to 0.05% of normal. In a tenth patient's liver, much higher activity was found (0.5% of normal). In this patient the residual enzyme had the same molecular weight as beta-galactosidase ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.1.240

    authors： Norden AG,O'Brien JS

    更新日期：1975-01-01 00:00:00

  • Identification of intracellular degradation intermediates of aldolase B by antiserum to the denatured enzyme.

    abstract：:A method has been developed that enables us to identify intracellular degradation intermediates of fructose-bisphosphate aldolase B (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase, EC 4.1.2.13). This method is based on the use of antibody against thoroughly denatured purified aldolase. This antibody ha...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.18.6114

    authors： Reznick AZ,Rosenfelder L,Shpund S,Gershon D

    更新日期：1985-09-01 00:00:00

  • Parallel RNA and DNA analysis after deep sequencing (PRDD-seq) reveals cell type-specific lineage patterns in human brain.

    abstract：:Elucidating the lineage relationships among different cell types is key to understanding human brain development. Here we developed parallel RNA and DNA analysis after deep sequencing (PRDD-seq), which combines RNA analysis of neuronal cell types with analysis of nested spontaneous DNA somatic mutations as cell lineag...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2006163117

    authors： Huang AY,Li P,Rodin RE,Kim SN,Dou Y,Kenny CJ,Akula SK,Hodge RD,Bakken TE,Miller JA,Lein ES,Park PJ,Lee EA,Walsh CA

    更新日期：2020-06-23 00:00:00

  • Blockade of the epidermal growth factor receptor tyrosine kinase suppresses tumorigenesis in MMTV/Neu + MMTV/TGF-alpha bigenic mice.

    abstract：:Overexpression of ErbB-2/Neu has been causally associated with mammary epithelial transformation. Here we report that blockade of the epidermal growth factor receptor (EGFR) kinase with AG-1478 markedly delays breast tumor formation in mouse mammary tumor virus (MMTV)/Neu + MMTV/transforming growth factor alpha bigeni...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.160564197

    authors： Lenferink AE,Simpson JF,Shawver LK,Coffey RJ,Forbes JT,Arteaga CL

    更新日期：2000-08-15 00:00:00

  • Resistance to docetaxel in prostate cancer is associated with androgen receptor activation and loss of KDM5D expression.

    abstract：:The androgen receptor (AR) plays an essential role in prostate cancer, and suppression of its signaling with androgen deprivation therapy (ADT) has been the mainstay of treatment for metastatic hormone-sensitive prostate cancer for more than 70 y. Chemotherapy has been reserved for metastatic castration-resistant pros...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1600420113

    authors： Komura K,Jeong SH,Hinohara K,Qu F,Wang X,Hiraki M,Azuma H,Lee GS,Kantoff PW,Sweeney CJ

    更新日期：2016-05-31 00:00:00

  • Multiplier ideal sheaves and existence of Kähler-Einstein metrics of positive scalar curvature.

    abstract：:To study C(0)a priori estimates for solutions to certain complex Monge-Ampère equations, I introduce a coherent sheaf of ideals and show that it satisfies various global algebrogeometric conditions, including a cohomology vanishing theorem. This technique is used to establish the existence of Kähler-Einstein metrics o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.19.7299

    authors： Nadel AM

    更新日期：1989-10-01 00:00:00

  • Regulation of scallop myosin by the regulatory light chain depends on a single glycine residue.

    abstract：:Specific Ca2+ binding and Ca2+ activation of ATPase activity in scallop myosin require a regulatory light chain (RLC) from regulated (molluscan or vertebrate smooth) myosin; hybrids containing vertebrate skeletal RLCs do not bind Ca2+ and their ATPase activity is inhibited. Chimeras between scallop and chicken skeleta...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.19.8762

    authors： Jancso A,Szent-Györgyi AG

    更新日期：1994-09-13 00:00:00

  • Expression of purine overproduction in a series of 8-azaguanine-resistant diploid human lymphoblast lines.

    abstract：:For study of the basis of an X-linked form of gout in man, several clonal lines deficient in hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) were selected from the human lymphoblast line WI-L2 by spontaneous and mutagen-induced resistance to 10 muM 8-azaguanine. Three groups could be defined: (1) clones wi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.7.2679

    authors： Lever JE,Nuki G,Seegmiller JE

    更新日期：1974-07-01 00:00:00

  • Raman resonance of electron donor/acceptor complex.

    abstract：:The resonance Raman excitation profiles of a number of charge transfer transitions in electron donor/acceptor complexes with tetracyanoethylene as acceptor in solution at room temperature are reported and compared with absorption and fluorescence spectra of these complexes. All complexes show distinct anomalies which ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.11.4196

    authors： Michaelian KH,Rieckhoff KE,Voigt EM

    更新日期：1975-11-01 00:00:00

  • Endothelial focal adhesion kinase mediates cancer cell homing to discrete regions of the lungs via E-selectin up-regulation.

    abstract：:Primary tumors secrete factors that alter the microenvironment of distant organs, rendering those organs as fertile soil for subsequent metastatic cancer cell colonization. Although the lungs are exposed to these factors ubiquitously, lung metastases usually develop as a series of discrete lesions. The underlining mol...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1100446108

    authors： Hiratsuka S,Goel S,Kamoun WS,Maru Y,Fukumura D,Duda DG,Jain RK

    更新日期：2011-03-01 00:00:00

  • A trans-acting RNA as a control switch in Escherichia coli: DsrA modulates function by forming alternative structures.

    abstract：:DsrA is an 87-nucleotide regulatory RNA of Escherichia coli that acts in trans by RNA-RNA interactions with two different mRNAs, hns and rpoS. DsrA has opposite effects on these transcriptional regulators. H-NS levels decrease, whereas RpoS (final sigma(s)) levels increase. Here we show that DsrA enhances hns mRNA tur...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.170281497

    authors： Lease RA,Belfort M

    更新日期：2000-08-29 00:00:00

  • Identification of frame-shift intermediate mutant cells.

    abstract：:Frame-shift mutations at microsatellites occur as a time-dependent function of polymerase errors followed by failure of postreplicational mismatch repair. A cell-culture system was developed that allows identification of intermediate mutant cells that carry the mutation on a single DNA strand after the initial DNA pol...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0437965100

    authors： Gasche C,Chang CL,Natarajan L,Goel A,Rhees J,Young DJ,Arnold CN,Boland CR

    更新日期：2003-02-18 00:00:00

  • Isolation and initial characterization of multiple species of T-lymphocyte subset cDNA clones.

    abstract：:A modified differential screening procedure was applied to analyze cDNA libraries of cloned helper T lymphocytes (Th) and cytolytic T lymphocytes (CTL). Negative and positive differential screening and RNA blot analysis were used to identify cDNA clones that were expressed preferentially in Th or CTL. Seven clones cor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.9.2896

    authors： Kwon BS,Kim GS,Prystowsky MB,Lancki DW,Sabath DE,Pan JL,Weissman SM

    更新日期：1987-05-01 00:00:00

  • Concomitant T-cell receptor alpha and delta gene rearrangements in individual T-cell precursors.

    abstract：:A debate has recently surfaced concerning the degree of precommitment attained by alpha beta and gamma delta T-cell precursors prior to T-cell receptor (TCR) gene rearrangement. It has been suggested that precursors may be precommitted to rearrange either alpha or delta genes, but not both, thus giving rise to alpha b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.14.5583

    authors： Thompson SD,Pelkonen J,Hurwitz JL

    更新日期：1990-07-01 00:00:00

  • Carbohydrate utilization during exercise after high-altitude acclimation: a new perspective.

    abstract：:At high altitude (HA), carbohydrate (CHO) is thought to be the preferred fuel because of its higher yield of ATP per mole of O2. We used indirect calorimetry and D-[6-3H]glucose infusions to determine total CHO and circulatory glucose utilization during exercise in HA-acclimated and sea level (SL) rats. We hypothesize...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.17.10288

    authors： McClelland GB,Hochachka PW,Weber JM

    更新日期：1998-08-18 00:00:00

  • Quantitative genomic analysis of RecA protein binding during DNA double-strand break repair reveals RecBCD action in vivo.

    abstract：:Understanding molecular mechanisms in the context of living cells requires the development of new methods of in vivo biochemical analysis to complement established in vitro biochemistry. A critically important molecular mechanism is genetic recombination, required for the beneficial reassortment of genetic information...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1424269112

    authors： Cockram CA,Filatenkova M,Danos V,El Karoui M,Leach DR

    更新日期：2015-08-25 00:00:00