Shortening the culture time in cytogenetic dosimetry using PCC-R assay.

Abstract:

:The fast assessment of the dose received by exposed persons is crucial in radiological accidents, so the 48 h of cell culture in conventional cytogenetic dosimetry in addition to some limitations after high doses becomes a disadvantage. The premature chromosome condensation (PCC) assay permits to analyse enough cells after high radiation exposure, and the score of PCC-R may reduce the culture time up to 40-42 h. Peripheral whole-blood samples were exposed to 1-10 Gy of gamma radiation and cultured during 40 and 42 h. No statistical difference between frequencies was obtained between 40, 42 and 48 h of culture time, and PCC index decreased with the increase of the dose and increased with the culture time. The protocol proposed allows reduce the culture time down to 40 or 42 h when using the PCC-R assay with adequate precision in dose estimation.

journal_name

Radiat Prot Dosimetry

authors

Romero I,Lamadrid AI,González JE,García O,Voisin P,Roy L

doi

10.1093/rpd/ncu258

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

424-9

issue

4

eissn

0144-8420

issn

1742-3406

pii

ncu258

journal_volume

163

pub_type

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