Abstract：
INTRODUCTION:The aim of this study was to show the emergence of the qnrD gene among fluoroquinolone-resistant Morganella morganii isolate. The occurrence of mutations in DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC,parE) genes was also investigated in this strain. METHODOLOGY:95 clinical Enterobacteria were screened for harbouring the qnrD gene. The clinical isolate of M. morganii was recovered from urine from a patient hospitalized in the urology unit at Fattouma Bourguiba Hospital, Tunisia. Antibiotic susceptibility was tested with the agar disk diffusion method. Quinolone susceptibility was studied with microbroth dilution technique. The investigations of plasmid mediated quinolone resistance (PMQR) and topoisomerases mutations were performed by polymerase chain reaction and nucleotide sequencing. RESULTS:This isolate showed high level of resistance to quinolones. The MIC with microbroth dilution technique was 512 μg/ml for norfloxacin, 256 μg/ml for ofloxacin and ciprofloxacin and 64μg/ml for levofloxacin.This strain was found to harbour the quinolone resistance determinant qnrD. In addition, this strain harboured two new gyrB mutations (S463A, S464Y) and one parC mutation (S80I). CONCLUSIONS:This is the first report in Tunisia of qnrD determinant and tow new gyrB muations in M. morganii. The nosocomial infection due to this proteeae invites further study of its epidemiologic evolution.
journal_name
Ann Clin Microbiol Antimicrobjournal_title
Annals of clinical microbiology and antimicrobialsauthors
Nasri Yaiche M,Denden Rafraf I,Guo Q,Mastouri M,Aouni M,Wang Mdoi
10.1186/s12941-014-0034-4subject
Has Abstractpub_date
2014-08-09 00:00:00pages
34issn
1476-0711pii
s12941-014-0034-4journal_volume
13pub_type
杂志文章abstract：BACKGROUND:Aquilaria crassna Pierre ex Lecomte has been traditionally used in Thailand for treatment of infectious diseases such as diarrhoea and skin diseases for a long time. The main objectives of this study were to examine antibacterial activity of the Aquilaria crassna leaf extract against Staphylococcus epidermid...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-12-20
更新日期：2013-08-20 00:00:00
abstract：BACKGROUND:Empiric antimicrobial therapy (EAMT) using imipenem/colistin is commonly prescribed as a first line therapy in critically ill patients with severe sepsis. We aimed to assess the appropriateness of prescribing imipenem/colistin as EAMT in ICU patients. METHODS:A 3-year observational prospective study include...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-018-0292-7
更新日期：2018-11-16 00:00:00
abstract：BACKGROUND:Chenopodium ambrosioides and Kielmeyera neglecta are plants traditionally used in Brazil to treat various infectious diseases. The study of the biological activities of these plants is of great importance for the detection of biologically active compounds. METHODS:Extracts from these plants were extracted w...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-11-20
更新日期：2012-07-28 00:00:00
abstract：:Mycobacterium bovis BCG vaccine significantly reduces the risk of tuberculosis by 50% and continues to be used to prevent tuberculosis around the world. However, it has been shown to be ineffective in some geographical regions. The existence of different BCG strains was described more than 60 years ago, these vary in ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-3-10
更新日期：2004-06-03 00:00:00
abstract：BACKGROUND:Diarrheal diseases continue to be the major cause of morbidity and mortality among children under 5 years. Salmonella and Shigella specious are the major enteric pathogen causing diarrhea among children worldwide. Examination of stool sample is the most sensitive method to diagnose diarrheal disease in child...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-018-0253-1
更新日期：2018-02-01 00:00:00
abstract：BACKGROUND:Aspergillus is a ubiquitous soil-dwelling fungus known to cause significant pulmonary infection in immunocompromised patients. The incidence of aspergillosis has increased during the past two decades and is a frequently lethal complication of acute leukemia patients that occurs following both chemotherapy an...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-5-17
更新日期：2006-07-27 00:00:00
abstract：BACKGROUND:Although tuberculosis is not uncommon among patients with myelodysplastic syndrome (MDS), only a few reports of such patients suffering from miliary tuberculosis (MT) exist. MT often presents as a fever of unknown origin and it is a curable disease, yet fatal if left untreated. CASE PRESENTATION:We report a...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章,评审
doi：10.1186/1476-0711-7-8
更新日期：2008-03-13 00:00:00
abstract：BACKGROUND:Cationic antimicrobial peptides (CAMPs) are well recognized to be promising as novel antimicrobial and antitumor agents. To obtain novel skeletons of CAMPs, we propose a simple strategy using acid-amide substitution (i.e. Glu→Gln, Asp→Asn) to confer net positive charge to natural non-antimicrobial sequences ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-10-11
更新日期：2011-03-22 00:00:00
abstract：:For centuries, plants have been used in traditional medicines and there has been recent interest in the chemopreventive properties of compounds derived from plants. In the present study, we investigated the antibutyrylcholinestrasic (anti-BuChE) and antioxidant (against some free radicals) activities of extracts from ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-10-32
更新日期：2011-08-31 00:00:00
abstract：BACKGROUND:Drug resistance in Mycobacterium tuberculosis (MTB) is a major health issue worldwide. Recently, next-generation sequencing (NGS) technology has begun to be used to detect resistance genes of MTB. We aimed to assess the clinical usefulness of Ion S5 NGS TB research panel for detecting MTB resistance in Korea...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-018-0300-y
更新日期：2019-01-03 00:00:00
abstract：BACKGROUND:Accurate measurement of the incidence of diarrhoeagenic E. coli in patients with diarrhoea is hindered by the current methods of detection and varies from country to country. In order to improve the diagnosis of diarrhoeagenic E. coli (DEC), we developed a set of multiplex TaqMan real-time PCRs designed to d...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-9-5
更新日期：2010-01-22 00:00:00
abstract：BACKGROUND:Accurate identification of isolates belonging to genus Enterococcus, Streptococcus, coagulase-negative Staphylococcus, and Lactococcus at the species level is necessary to provide a better understanding of their pathogenic potential, to aid in making clinical decisions, and to conduct epidemiologic investiga...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-11-31
更新日期：2012-11-27 00:00:00
abstract：BACKGROUND:This study aimed to evaluate in vitro susceptibility to ceftobiprole of clinical strains identified from prosthetic joint infections (PJIs) compared to that of the associations currently recommended for post-operative empirical antibiotic therapy (PEAT) (vancomycin with either cefepime, third-generation ceph...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-020-00351-5
更新日期：2020-03-21 00:00:00
abstract：BACKGROUND:Persistent bacteremia occurs in at least 30% of patients with Staphylococcus aureus bloodstream infection (SAB) and may be attributable to a dysregulated host immune response. Neutrophils interact with a variety of S. aureus microbial factors, including lipoteichoic acid (LTA), to activate phagocytic functio...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-020-00392-w
更新日期：2020-11-03 00:00:00
abstract：BACKGROUND:The evolving epidemiology of methicillin resistant Staphylococcus aureus (MRSA) is characterized by the emergence of infections caused by non multiresistant MRSA carrying staphylococcal chromosomal cassette (SCC)mec IV or V in the healthcare settings. A molecular epidemiological analysis of non multiresistan...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-11-17
更新日期：2012-06-19 00:00:00
abstract：BACKGROUND:Multidrug resistant Pseudomonas aeruginosa harbours integrons and other mobile genetic elements such as plasmids and transposons, which easily disseminate antibiotic resistance genes among clinical strains of P. aeruginosa. METHODOLOGY:Plasmid extraction of 54 clinical isolates of P. aeruginosa was carried ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-12-29
更新日期：2013-10-21 00:00:00
abstract：BACKGROUND:Urinary tract infections (UTIs) remain the common infections diagnosed in outpatients as well as hospitalized patients. Current knowledge on antimicrobial susceptibility pattern is essential for appropriate therapy. Extended-Spectrum beta-Lactamase (ESBL) producing bacteria may not be detected by routine dis...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-6-4
更新日期：2007-03-23 00:00:00
abstract：BACKGROUND:A growing number of rapid Helicobacter pylori antibody tests are commercially available now, however, some of these tests are often used without sufficient evaluation. The aim of this study was to evaluate the performance of a commercially available rapid whole-blood immunoassay (gabControl(®) H. pylori; gab...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-016-0161-1
更新日期：2016-08-08 00:00:00
abstract：BACKGROUND:Drug therapy is recommended in conjunction with surgery in treatment of actinomycetoma. The specific prescription depends on the type of bacteria (actinomycetoma) or fungi (eumycetoma) causing the disease and their in vitro antimicrobial susceptibility. OBJECTIVES:To investigate the antimicrobial susceptibi...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-10-24
更新日期：2011-06-06 00:00:00
abstract：BACKGROUND:Four medicinal plants (Chrozophora hierosolymitana Spreng, Chrysanthemum leucanthemum L., Ephedra gerardiana Wall. ex Stapf, and Quercus dilatata L.) used by indigenous healers to treat various infectious diseases were selected for the present study. The major objective of the present study was isolation and...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-11-11
更新日期：2012-05-03 00:00:00
abstract：BACKGROUND:The consumption of carbapenems has increased worldwide, together with the increase in resistant gram negative bacilli. Subsequently, the prevalence of carbapenem-resistant Acinetobacter infections has increased rapidly and become a significant problem particularly in intensive care unit patients. The aim of ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-13-7
更新日期：2014-01-09 00:00:00
abstract：BACKGROUND:Vaginitis is among the most common conditions women are seeking medical care for. Although these infections can easily be treated, the relapse rate is high. This may be due to inadequate use of the diagnostic potential. METHODS:We evaluated the misjudgement rate of the aetiology of vaginal complaints. A tot...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-5-4
更新日期：2006-02-17 00:00:00
abstract：BACKGROUND:Antibiotic resistance is a defense mechanism, harbored by pathogens to survive under unfavorable conditions. Among several antibiotic resistant microbial consortium, Staphylococcus aureus is one of the most havoc microorganisms. Staphylococcus aureus encodes a unique enzyme 6-hydroxymethyl-7,8-dihydropterin ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/s12941-018-0266-9
更新日期：2018-04-02 00:00:00
abstract：:Stenotrophomonas maltophilia (S. maltophilia) has recently emerged as an important nosocomial pathogen. Treatment of invasive infections caused by this organism is difficult as the bacterium is frequently resistant to a wide range of commonly used antimicrobials. Trimethoprim-sulfamethoxazole (TMP - SXT) is recommende...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-5-23
更新日期：2006-09-18 00:00:00
abstract：BACKGROUND:Diarrhoeal diseases constitute a major public health problem, particularly in the developing world, where the rate of mortality and morbidity is very high. The purpose of this study was to conduct a 2 years and 3 months study in order to determine the prevalence of five enteropathogen diarrheogenic agents in...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-6-17
更新日期：2007-12-28 00:00:00
abstract：BACKGROUND:Escherichia coli is the commonest cause of community and nosocomial urinary tract infection (UTI). Antibiotic treatment is usually empirical relying on susceptibility data from local surveillance studies. We therefore set out to determine levels of resistance to 8 commonly used antimicrobial agents amongst a...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-7-13
更新日期：2008-06-18 00:00:00
abstract：:Resistance to beta-lactam antibiotics in enteric Gram-negative bacilli may be difficult to detect using standard methods of either Kirby-Bauer disc diffusion (KBDD) or broth dilution for minimal inhibitory concentration (MIC). This difficulty is due to genetic differences in resistance determinants, differences in lev...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-3-13
更新日期：2004-07-15 00:00:00
abstract：BACKGROUND:Clinical microbiology laboratories have to accurately identify clinical microbes. However, some isolates are difficult to identify by the automated biochemical text platforms, which are called "difficult-to-identify" microbes in this study. Therefore, the ability of 16S ribosomal DNA (16S rDNA) and internal ...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-13-1
更新日期：2014-01-03 00:00:00
abstract：BACKGROUND:Non-typhoidal Salmonella (NTS) is increasingly recognized as an important pathogen associated with bacteraemia especially in immunosuppressed patients. However, there is limited data specifically describing the clinical characteristics and outcome amongst the immunosuppressed patients. METHODS:A total of 56...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-8-15
更新日期：2009-05-18 00:00:00
abstract：BACKGROUND:The aim of the present work was to evaluate the antibacterial effect of 3,4-DHPEA-EA (methyl-4-(2-(3,4-dihydroxyphenethoxy)-2-oxoethyl)-3-formyl-2-methyl-3,4-dihydro-2H-pyran-5-carboxylate), a derivate of oleuropein, against a range of Gram-positive bacteria, including ATCC strains, food and clinical isolate...
journal_title：Annals of clinical microbiology and antimicrobials
pub_type： 杂志文章
doi：10.1186/1476-0711-13-24
更新日期：2014-07-01 00:00:00
