Abstract:
BACKGROUND:Clinical microbiology laboratories have to accurately identify clinical microbes. However, some isolates are difficult to identify by the automated biochemical text platforms, which are called "difficult-to-identify" microbes in this study. Therefore, the ability of 16S ribosomal DNA (16S rDNA) and internal transcribed spacer 2 (ITS2) sequencing to identify these "difficult-to-identify" bacteria and fungi was assessed in this study. METHODS:Samples obtained from a teaching hospital over the past three years were examined. The 16S rDNA of four standard strains, 18 clinical common isolates, and 47 "difficult-to-identify" clinical bacteria were amplified by PCR and sequenced. The ITS2 of eight standard strains and 31 "difficult-to-identify" clinical fungi were also amplified by PCR and sequenced. The sequences of 16S rDNA and ITS2 were compared to reference data available in GenBank by using the BLASTN program. These microbes were identified according to the percentage of similarity to reference sequences of strains in GenBank. RESULTS:The results from molecular sequencing methods correlated well with automated microbiological identification systems for common clinical isolates. Sequencing results of the standard strains were consistent with their known phenotype. Overall, 47 "difficult-to-identify" clinical bacteria were identified as 35 genera or species by sequence analysis (with 10 of these identified isolates first reported in clinical specimens in China and two first identified in the international literature). 31 "difficult-to-identify" clinical fungi tested could be identified as 15 genera or species by sequence analysis (with two of these first reported in China). CONCLUSIONS:Our results show the importance of 16S rDNA and internal ITS2 sequencing for the molecular identification of "difficult-to-identify" bacteria and fungi. The development of this method with advantages of convenience, availability, and cost-effectiveness will make it worth extending into clinical practice in developing countries.
journal_name
Ann Clin Microbiol Antimicrobjournal_title
Annals of clinical microbiology and antimicrobialsauthors
Cheng C,Sun J,Zheng F,Wu K,Rui Ydoi
10.1186/1476-0711-13-1subject
Has Abstractpub_date
2014-01-03 00:00:00pages
1issn
1476-0711pii
1476-0711-13-1journal_volume
13pub_type
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journal_title:Annals of clinical microbiology and antimicrobials
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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journal_title:Annals of clinical microbiology and antimicrobials
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journal_title:Annals of clinical microbiology and antimicrobials
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doi:10.1186/1476-0711-5-4
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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journal_title:Annals of clinical microbiology and antimicrobials
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journal_title:Annals of clinical microbiology and antimicrobials
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pub_type: 杂志文章,评审
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-1-1
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pub_type: 杂志文章
doi:10.1186/1476-0711-10-38
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,多中心研究
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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journal_title:Annals of clinical microbiology and antimicrobials
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更新日期:2018-11-16 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-16
更新日期:2011-05-05 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-10-24
更新日期:2011-06-06 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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更新日期:2020-03-30 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-11-20
更新日期:2012-07-28 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-11-17
更新日期:2012-06-19 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章,评审
doi:10.1186/s12941-018-0289-2
更新日期:2018-10-10 00:00:00
abstract:BACKGROUND:Non-typhoidal Salmonella (NTS) is increasingly recognized as an important pathogen associated with bacteraemia especially in immunosuppressed patients. However, there is limited data specifically describing the clinical characteristics and outcome amongst the immunosuppressed patients. METHODS:A total of 56...
journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
doi:10.1186/1476-0711-8-15
更新日期:2009-05-18 00:00:00
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journal_title:Annals of clinical microbiology and antimicrobials
pub_type: 杂志文章
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更新日期:2015-09-02 00:00:00