Epidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility.

abstract：:Mutant KRAS colorectal cancer (CRC) cells release protein-laden exosomes that can alter the tumor microenvironment. To test whether exosomal RNAs also contribute to changes in gene expression in recipient cells, and whether mutant KRAS might regulate the composition of secreted microRNAs (miRNAs), we compared small RN...

journal_title：eLife

authors： Cha DJ,Franklin JL,Dou Y,Liu Q,Higginbotham JN,Demory Beckler M,Weaver AM,Vickers K,Prasad N,Levy S,Zhang B,Coffey RJ,Patton JG

更新日期：2015-07-01 00:00:00

abstract：:The sequential activation of neurons has been observed in various areas of the brain, but in no case is the underlying network structure well understood. Here we examined the circuit anatomy of zebra finch HVC, a cortical region that generates sequences underlying the temporal progression of the song. We combined seri...

journal_title：eLife

authors： Kornfeld J,Benezra SE,Narayanan RT,Svara F,Egger R,Oberlaender M,Denk W,Long MA

更新日期：2017-03-27 00:00:00

abstract：:Lamina-associated polypeptide 1 (LAP1) resides at the nuclear envelope and interacts with Torsins, poorly understood endoplasmic reticulum (ER)-localized AAA+ ATPases, through a conserved, perinuclear domain. We determined the crystal structure of the perinuclear domain of human LAP1. LAP1 possesses an atypical AAA+ f...

journal_title：eLife

authors： Sosa BA,Demircioglu FE,Chen JZ,Ingram J,Ploegh HL,Schwartz TU

更新日期：2014-08-22 00:00:00

abstract：:Despite their importance in sexual differentiation and reproduction, Y chromosome genes are rarely described because they reside in repeat-rich regions that are difficult to study. Here, we show that Guy1, a unique Y chromosome gene of a major urban malaria mosquito Anopheles stephensi, confers 100% female lethality w...

journal_title：eLife

authors： Criscione F,Qi Y,Tu Z

更新日期：2016-09-20 00:00:00

abstract：:Computational modeling of behavior has revolutionized psychology and neuroscience. By fitting models to experimental data we can probe the algorithms underlying behavior, find neural correlates of computational variables and better understand the effects of drugs, illness and interventions. But with great power comes ...

journal_title：eLife

authors： Wilson RC,Collins AG

更新日期：2019-11-26 00:00:00

abstract：:A key challenge in precise genome editing is the low efficiency of homology-directed repair (HDR). Here we describe a strategy for increasing the efficiency of HDR in cells by using a chromatin donor template instead of a naked DNA donor template. The use of chromatin, which is the natural form of DNA in the nucleus, ...

journal_title：eLife

authors： Cruz-Becerra G,Kadonaga JT

更新日期：2020-04-28 00:00:00

abstract：:Transcription at individual genes in single cells is often pulsatile and stochastic. A key question emerges regarding how this behaviour contributes to tissue phenotype, but it has been a challenge to quantitatively analyse this in living cells over time, as opposed to studying snap-shots of gene expression state. We ...

journal_title：eLife

authors： Featherstone K,Hey K,Momiji H,McNamara AV,Patist AL,Woodburn J,Spiller DG,Christian HC,McNeilly AS,Mullins JJ,Finkenstädt BF,Rand DA,White MR,Davis JR

更新日期：2016-02-01 00:00:00

abstract：:The basal ganglia are known to be involved in the planning, execution and control of gripping force and movement vigour. Here we aim to define the nature of the basal ganglia control signal for force and to decode gripping force based on local field potential (LFP) activities recorded from the subthalamic nucleus (STN...

journal_title：eLife

authors： Tan H,Pogosyan A,Ashkan K,Green AL,Aziz T,Foltynie T,Limousin P,Zrinzo L,Hariz M,Brown P

更新日期：2016-11-18 00:00:00

abstract：:Oomycete pathogens secrete host cell-entering effector proteins to manipulate host immunity during infection. We previously showed that PsAvh52, an early-induced RxLR effector secreted from the soybean root rot pathogen, Phytophthora sojae, could suppress plant immunity. Here, we found that PsAvh52 is required for ful...

journal_title：eLife

authors： Li H,Wang H,Jing M,Zhu J,Guo B,Wang Y,Lin Y,Chen H,Kong L,Ma Z,Wang Y,Ye W,Dong S,Tyler B,Wang Y

更新日期：2018-10-22 00:00:00

abstract：:The multi-domain splicing factor RBM5 regulates the balance between antagonistic isoforms of the apoptosis-control genes FAS/CD95, Caspase-2 and AID. An OCRE (OCtamer REpeat of aromatic residues) domain found in RBM5 is important for alternative splicing regulation and mediates interactions with components of the U4/U...

journal_title：eLife

authors： Mourão A,Bonnal S,Soni K,Warner L,Bordonné R,Valcárcel J,Sattler M

更新日期：2016-11-29 00:00:00

abstract：:Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators, and biological effects of terrein production were studied in the fun...

journal_title：eLife

authors： Gressler M,Meyer F,Heine D,Hortschansky P,Hertweck C,Brock M

更新日期：2015-07-14 00:00:00

abstract：:TMEM16A is a ligand-gated anion channel that is activated by intracellular Ca2+. This channel comprises two independent pores and closely apposed Ca2+ binding sites that are contained within each subunit of a homodimeric protein. Previously we characterized the influence of positively charged pore-lining residues on a...

journal_title：eLife

authors： Lam AK,Dutzler R

更新日期：2018-10-12 00:00:00

abstract：:Understanding the relationships between different properties of data, such as whether a genome or connectome has information about disease status, is increasingly important. While existing approaches can test whether two properties are related, they may require unfeasibly large sample sizes and often are not interpret...

journal_title：eLife

authors： Vogelstein JT,Bridgeford EW,Wang Q,Priebe CE,Maggioni M,Shen C

更新日期：2019-01-15 00:00:00

abstract：:Human-based modelling and simulations are becoming ubiquitous in biomedical science due to their ability to augment experimental and clinical investigations. Cardiac electrophysiology is one of the most advanced areas, with cardiac modelling and simulation being considered for virtual testing of pharmacological therap...

journal_title：eLife

authors： Tomek J,Bueno-Orovio A,Passini E,Zhou X,Minchole A,Britton O,Bartolucci C,Severi S,Shrier A,Virag L,Varro A,Rodriguez B

更新日期：2019-12-24 00:00:00

abstract：:Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...

journal_title：eLife

authors： Perl O,Nahum N,Belelovsky K,Haddad R

更新日期：2020-02-07 00:00:00

abstract：:Convenient, efficient and fast whole-brain delivery of transgenes presents a persistent experimental challenge in neuroscience. Recent advances demonstrate whole-brain gene delivery by retro-orbital injection of virus, but slow and sparse expression and the large injection volumes required make this approach cumbersom...

journal_title：eLife

authors： Hamodi AS,Martinez Sabino A,Fitzgerald ND,Moschou D,Crair MC

更新日期：2020-05-18 00:00:00

abstract：:Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocy...

journal_title：eLife

authors： Manzoni G,Marinach C,Topçu S,Briquet S,Grand M,Tolle M,Gransagne M,Lescar J,Andolina C,Franetich JF,Zeisel MB,Huby T,Rubinstein E,Snounou G,Mazier D,Nosten F,Baumert TF,Silvie O

更新日期：2017-05-16 00:00:00

abstract：:Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...

journal_title：eLife

authors： Yamashita T,Petersen CCh

更新日期：2016-06-21 00:00:00

abstract：:Alzheimer's and Parkinson's disease are late onset neurodegenerative diseases that will require therapy over decades to mitigate the effects of disease-driving proteins such tau and α-synuclein (α-Syn). Previously we found that TRIM28 regulates the levels and toxicity of α-Syn and tau (journal_title：eLife

authors： Rousseaux MW,Revelli JP,Vázquez-Vélez GE,Kim JY,Craigen E,Gonzales K,Beckinghausen J,Zoghbi HY

更新日期：2018-06-04 00:00:00

abstract：:The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding i...

journal_title：eLife

authors： Joseph RM,Sun JS,Tam E,Carlson JR

更新日期：2017-03-23 00:00:00

abstract：:Animals can effortlessly adapt their behavior by generalizing from past aversive experiences, allowing to avoid harm in novel situations. We studied how visual information was sampled by eye-movements during this process called fear generalization, using faces organized along a circular two-dimensional perceptual cont...

journal_title：eLife

authors： Kampermann L,Wilming N,Alink A,Büchel C,Onat S

更新日期：2019-10-22 00:00:00

abstract：:In eukaryotes, most integral membrane proteins are synthesized, integrated into the membrane, and folded properly in the endoplasmic reticulum (ER). We screened the mutants affecting rhabdomeric expression of rhodopsin 1 (Rh1) in the Drosophila photoreceptors and found that dPob/EMC3, EMC1, and EMC8/9, Drosophila homo...

journal_title：eLife

authors： Satoh T,Ohba A,Liu Z,Inagaki T,Satoh AK

更新日期：2015-02-26 00:00:00

abstract：:In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells wi...

journal_title：eLife

authors： Schanzenbächer CT,Langer JD,Schuman EM

更新日期：2018-02-15 00:00:00

abstract：:Temporary circuits amplify spontaneous activity in the visual system of neonatal rats. ...

journal_title：eLife

authors： Fogerson PM,Huguenard JR

更新日期：2016-10-11 00:00:00

abstract：:Precise and efficient manipulation of genes is crucial for understanding the molecular mechanisms that govern human hematopoiesis and for developing novel therapies for diseases of the blood and immune system. Current methods do not enable precise engineering of complex genotypes that can be easily tracked in a mixed ...

journal_title：eLife

authors： Bak RO,Dever DP,Reinisch A,Cruz Hernandez D,Majeti R,Porteus MH

更新日期：2017-09-28 00:00:00

abstract：:We have previously demonstrated that checkpoint kinase 2 (CHK2) is a critical negative regulator of androgen receptor (AR) transcriptional activity, prostate cancer (PCa) cell growth, and androgen sensitivity. We have now uncovered that the AR directly interacts with CHK2 and ionizing radiation (IR) increases this int...

journal_title：eLife

authors： Ta HQ,Dworak N,Ivey ML,Roller DG,Gioeli D

更新日期：2020-06-24 00:00:00

abstract：:Mature microRNAs (miRNAs) are processed from primary transcripts (pri-miRNAs), and their expression is controlled at transcriptional and post-transcriptional levels. However, how regulation at multiple levels achieves precise control remains elusive. Using published and new datasets, we profile a time course of mature...

journal_title：eLife

authors： Zhou L,Lim MYT,Kaur P,Saj A,Bortolamiol-Becet D,Gopal V,Tolwinski N,Tucker-Kellogg G,Okamura K

更新日期：2018-07-19 00:00:00

abstract：:Innate lymphoid cells (ILCs) are recently identified lymphocytes that limit infection and promote tissue repair at mucosal surfaces. However, the pathways underlying ILC development remain unclear. Here we show that the transcription factor NFIL3 directs the development of a committed bone marrow precursor that differ...

journal_title：eLife

authors： Yu X,Wang Y,Deng M,Li Y,Ruhn KA,Zhang CC,Hooper LV

更新日期：2014-10-13 00:00:00

abstract：:Social rank differentially influences how male and female mice respond to chronic stress. ...

journal_title：eLife

authors： Bangasser DA,Sanchez EO

更新日期：2020-11-20 00:00:00

abstract：:In eukaryotes, intra-chromosomal recombination generates DNA circles, but little is known about how cells react to them. In yeast, partitioning of such circles to the mother cell at mitosis ensures their loss from the population but promotes replicative ageing. Nevertheless, the mechanisms of partitioning are debated....

journal_title：eLife

authors： Denoth-Lippuner A,Krzyzanowski MK,Stober C,Barral Y

更新日期：2014-11-17 00:00:00