Abstract：
:Slice cultures from the visual cortex of young rats were prepared using the roller culture technique (Gähwiler 1984). After 10 days in vitro the cortical cultures flattened to 1-3 cell layers, surviving for up to 12 weeks. The cultures were organotypically organized, the typical layered structure of the cortex was preserved. The neuronal composition of slice cultures was studied using intracellular staining, Golgi impregnation and GABA immunohistochemistry. Both pyramidal cells and several types of nonpyramidal cells were identified in the slice cultures. Electrophysiological recordings showed that the electrical properties of cells in culture were similar to those measured in acute slice preparations; for some cells, however, the spontaneous activity was higher. The maintained activity was strongly increased by application of the GABA antagonist bicuculline and decreased by GABA, suggesting that GABAergic inhibition is present in these preparations. We could observe the postnatal maturation of some characteristic morphological features in culture. For example, pyramidal cells in 6 day-old rats in situ have very short basal dendrites with growth-cones, and the dendrites are free of spines. After 2-3 weeks in culture growth-cones were no longer observed. Instead, the cells had developed a large basal dendritic field and the dendrites were covered with spines. Slice cultures therefore may provide a useful tool for physiological, anatomical, pharmacological and developmental studies of cortical neurons in an organotypical environment.
journal_name
Exp Brain Resjournal_title
Experimental brain researchauthors
Caeser M,Bonhoeffer T,Bolz Jdoi
10.1007/BF00274981subject
Has Abstractpub_date
1989-01-01 00:00:00pages
234-44issue
2eissn
0014-4819issn
1432-1106journal_volume
77pub_type
杂志文章abstract：:The synapsins are presynaptic membrane-associated proteins involved in neurotransmitter release. They are differentially expressed in tissues and cells of the central and peripheral nervous system. In vestibular end organs of mammals, synapsin I-like immunoreactivity has been reported in efferent and afferent terminal...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-004-2194-5
更新日期：2005-04-01 00:00:00
abstract：:Efferent and afferent connections of the visually responsive cortex (area anteromedial, AM) located in the anterior portion of area 18b were studied with degeneration and horseradish peroxidase (HRP) methods following small lesions and HRP injections into this area. Degenerating axons, terminals and retrogradely HRP-l...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00237996
更新日期：1984-01-01 00:00:00
abstract：:Recent results on the nature and limits of multisensory enhancement are inconsistent when stimuli are presented across spatial regions. We presented visual, tactile and visuotactile stimuli to participants in two speeded response tasks. Each unisensory stimulus was presented to either the left or right hemispace, and ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-010-2515-9
更新日期：2011-09-01 00:00:00
abstract：:We investigated the influence of gaze elevation on judging the possibility of passing under high obstacles during pitch body tilts, while stationary, in absence of allocentric cues. Specifically, we aimed at studying the influence of egocentric references upon geocentric judgements. Seated subjects, orientated at vari...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-008-1589-0
更新日期：2009-02-01 00:00:00
abstract：:We explored changes in the cyclical two-finger force performance task caused by turning visual feedback off performed either by the index and middle fingers of the dominant hand or by two index fingers of two persons. Based on an earlier study, we expected drifts in finger force amplitude and midpoint without a drift ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-018-5179-5
更新日期：2018-03-01 00:00:00
abstract：:A 3-day incremental protocol was conducted with the aim of adapting human subjects to make head movements comfortably during 30 RPM centrifugation. With motion sickness as a potentially limiting factor, the protocol was designed using a quantitative motion sickness model based upon the neural mismatch sensory conflict...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-008-1415-8
更新日期：2008-08-01 00:00:00
abstract：:The amygdala has been demonstrated to contribute to pain-related behavior and food preference. Here, the effect of pain on food preference and food-matched visual-cue memory, in the presence or absence of a basolateral amygdala (BLA) lesion, has been evaluated using a novel innovative apparatus and protocol. Forty adu...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-020-05961-1
更新日期：2020-10-26 00:00:00
abstract：:Extracellular recordings were made from neurones in the ventromedial and parafasicular nuclei of the rat thalamus, many of which had demonstrable capsular or caudate projections. These cells responded to electrical stimulation of the ipsilateral substantia nigra with a short latency (4 ms) inhibition presumed to be mo...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00236662
更新日期：1980-01-01 00:00:00
abstract：:Is it possible to learn to perform a motor sequence without awareness of the sequence? In two experiments, we presented participants with the most elementary sequence: an alternation between two options. We used a double-step pointing task in which the final position of the target alternated between two quite similar ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-016-4566-z
更新日期：2016-06-01 00:00:00
abstract：:The present study tested the idea that the visuomotor systems mediating prehension do not have independent access to pictorial cues processed by perceptual mechanisms. Individuals with visual form agnosia, whose perceptual systems are compromised but who have intact visuomotor control, were examined to determine wheth...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/pl00005731
更新日期：1997-08-01 00:00:00
abstract：:The intracortical arborizations of thalamocortical fibers arising from the ventroposterolateral (VPL) nucleus in the cat were studied following intra-axonal injections of horseradish peroxidase (HRP). The axons were impaled 1.5 to 3 mm below the surface of the cortex, identified electrophysiologically by stimulating t...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00236304
更新日期：1987-01-01 00:00:00
abstract：:Cell responses to complex visual patterns such as compositions of broad-band (non-monochromatic) colour areas are presented. Patterns were scanned over the receptive field, and cell response at each point was recorded. "Response patterns" were constructed which display the cell transform of the stimulus pattern. Parvo...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00239571
更新日期：1982-01-01 00:00:00
abstract：:Does the brain use a separate internal model for cursor mechanics during visuomotor adaptation? We compared the amount of adaptation and transfer to the opposite arm when subjects reached the targets under different viewing conditions of the arm during reaching. If the brain forms separate models, we predict a differe...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-010-2155-0
更新日期：2010-05-01 00:00:00
abstract：:Motor imagery (MI - i.e., the mental representation of an action without physically executing it) stimulates brain motor networks and promotes motor learning after spinal cord injury (SCI). An interesting issue is whether the brain networks controlling MI are being reorganized with reference to spared motor functions....
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-014-4114-7
更新日期：2015-01-01 00:00:00
abstract：:Extracellular recordings were made from single on-center optic tract fibres in cats. Light spots of 10sec duration were focussed on the center of the receptive field under 1cd/m2 adaptation luminance by diffuse light. In the maintained discharges at 1 cd/m2, the shape of inter-spike interval histograms (IH) tended to ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00235016
更新日期：1976-01-26 00:00:00
abstract：:In this study, we investigated the neural basis of virtual time to contact (VTC) and the hypothesis that VTC provides predictive information for future postural instability. A novel approach to differentiate stable pre-falling and transition-to-instability stages within a single postural trial while a subject was perf...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-009-1956-5
更新日期：2009-10-01 00:00:00
abstract：:Weight illusions--where one object feels heavier than an identically weighted counterpart--have been the focus of many recent scientific investigations. The most famous of these illusions is the 'size-weight illusion', where a small object feels heavier than an identically weighted, but otherwise similar-looking, larg...
journal_title：Experimental brain research
pub_type： 杂志文章,评审
doi：10.1007/s00221-014-3926-9
更新日期：2014-06-01 00:00:00
abstract：:The present experiment assessed the locomotor response to a low dose (1 mg/kg) of systemic D-amphetamine in rats with cytotoxic lesions of the retrohippocampus (entorhinal and extra-subicular cortices), compared with vehicle-operated shams and unoperated controls. Under spontaneous and saline conditions, both the sham...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00241131
更新日期：1995-01-01 00:00:00
abstract：:Cortical migration defects are often associated with epilepsy. In mesial temporal lobe epilepsy (MTLE), granule cell dispersion (GCD), a migration defect of dentate granule cells, is frequently observed. Little is known how GCD develops and to which extent it contributes to the development of seizure activity. Since t...
journal_title：Experimental brain research
pub_type： 杂志文章,评审
doi：10.1007/s00221-009-1948-5
更新日期：2010-01-01 00:00:00
abstract：:Local anesthetic may cause neurotoxicity in developing neurons. In this study, we examined the molecular mechanisms of microRNA-210 (miR-210) in regulating bupivacaine-induced dorsal root ganglia (DRG) neurotoxicity in vitro. Young mouse (P30) DRG explants were cultured in vitro and treated with 5 mM bupivacaine to in...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-015-4513-4
更新日期：2016-04-01 00:00:00
abstract：:Wheat germ agglutinin-horseradish peroxidase (WGA-HRP) conjugate was injected into the lumbar or cervical spinal cord of adult cats in order to examine its usefulness as an anterograde tracer of long ascending spinal tracts. Heavily labeled fibers and terminal-like structures were found in restricted regions of the gr...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00237154
更新日期：1983-01-01 00:00:00
abstract：:The contribution of different cortical regions to visuospatial attention can be probed with the help of perturbation techniques, such as transcranial magnetic stimulation (TMS). Repetitive TMS (rTMS) has also been suggested as a tool for the therapy of brain injuries, by adjusting the neural excitability of injured or...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-008-1449-y
更新日期：2008-07-01 00:00:00
abstract：:When someone grasps an object, the grasp depends on the intended object manipulation and usually facilitates it. If several object manipulation steps are planned, the first step has been reported to primarily determine the grasp selection. We address whether the grasp can be aligned to the second step, if the second s...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-017-4892-9
更新日期：2017-05-01 00:00:00
abstract：:The present report describes a bidirectional mechanical stimulation technique of cristae ampullares in the cat. Bimodal field potentials are seen in the vestibular nerve and nucleus after an excitatory stimulus. This technique should allow an in vivo and selective study of the vestibular receptors in mammals. ...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00237804
更新日期：1980-01-01 00:00:00
abstract：:In the present article a number of comparative lesion studies in two inbred strains of mice (C57BL/6 and DBA/2) with different levels of radial maze performance are reviewed. The effects of lesions in several brain areas on maze learning were investigated, thus revealing strain differences in the neural circuitry subs...
journal_title：Experimental brain research
pub_type： 杂志文章,评审
doi：10.1007/s002210050542
更新日期：1998-11-01 00:00:00
abstract：:We investigated the accuracy with which, in the absence of vision, one can reach again a 2D target location that had been previously identified by a guided movement. A robotic arm guided the participant's hand to a target (locating motion) and away from it (homing motion). Then, the participant pointed freely toward t...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-004-1833-1
更新日期：2004-07-01 00:00:00
abstract：:In sensory saltation, first reported by Geldard and Sherrick (Science 178:178-179, 1972), a stimulus is displaced towards a second one following closely in time and space as a function of the delay between the stimuli. The distance between stimulus locations is restricted by the extension of sensory fields in the prim...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-005-0190-z
更新日期：2006-03-01 00:00:00
abstract：:Sensitivity to many visual stimuli, and, in particular, image displacement, is reduced during a change in fixation (saccade) compared to when the eye is still. In these experiments, we studied the sensitivity of observers to ecologically relevant image translations of large, complex, real world scenes either during ho...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-009-2120-y
更新日期：2010-04-01 00:00:00
abstract：:Dual task paradigms can be used to examine the interactions between cognition and the control of posture and gait. Measuring and interpreting changes in dual task performance is challenging, however, because many factors can influence performance. This study examined the effects of instructed focus and walking task di...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/s00221-010-2429-6
更新日期：2010-11-01 00:00:00
abstract：:Pineal glands were collected from superior cervical ganglionectomized (SCGX) and sham-treated rats at time intervals following surgical manipulation. Pineal noradrenaline (NA) levels were quantitated by a radioisotopic-enzyme technique, or the pineals were examined by histofluorescence or electron microscopic techniqu...
journal_title：Experimental brain research
pub_type： 杂志文章
doi：10.1007/BF00238713
更新日期：1978-07-14 00:00:00
