Abstract：
:Chitosaccharides (CS) of varied size were prepared from shrimp shell through sequential catalysis, using crude protease and chitinase enzymes immobilized on agar beads. In the optimized state, immobilization yield and activity yield for protease were 84% and 62%, and for chitinase were 75% and 57%, respectively. Immobilized protease and chitinase treatment improved CS yields (101 μg/ml) and retained 63% and 52% of activities after 10 reuses, respectively. Stronger radical-scavenging activity (RSA) of CS against ABTS, DPPH and hydroxyl radical was noted with EC50 values 19.1, 26.4 and 29.6 μg/ml, respectively. Peroxyl and superoxide RSAs of 96.8% and 88.6% were noticed at 70 μg/ml of CS. Singlet oxygen quenching, reducing power and ferrous ion-chelating activities of CS were also pronounced. CS reasonably reduced oxidative damage of DNA, protein and RBC by inhibiting H2O2 and AAPH radicals. Reversible CS-DNA condensation leads to DNA stabilization without changing its conformation and advocates its employment in gene therapy.
journal_name
Food Chemjournal_title
Food chemistryauthors
Halder SK,Jana A,Das A,Paul T,Das Mohapatra PK,Pati BR,Mondal KCdoi
10.1016/j.foodchem.2014.02.115subject
Has Abstractpub_date
2014-09-01 00:00:00pages
325-34eissn
0308-8146issn
1873-7072pii
S0308-8146(14)00300-8journal_volume
158pub_type
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