Abstract:
:Dextran or xanthan were used as model exocellular polysaccharides (EPS) to compare the extraction efficiency of EPS from skim milk acid gels using three different protocols. Extraction yields, residual protein concentrations and the macromolecular properties of extracted EPS were determined. For both model EPS, the highest extraction yield (∼80%) was obtained when samples were heated in acidic conditions at the first step of extraction (Protocol 1). Protocols that contained steps of acid/ethanol precipitation without heating (Protocols 2 and 3) show lower extraction yields (∼55%) but allow a better preservation of the EPS macromolecular properties. Changing the pH of acid gels up to 7 before extraction (Protocol 3) improved the extraction yield of anionic EPS without effect on the macromolecular properties of EPS. Protocol 1 was then applied for the quantification of EPS produced during the yogurt fermentation, while Protocol 3 was dedicated to their macromolecular characterization.
journal_name
Food Chemjournal_title
Food chemistryauthors
Nguyen AT,Nigen M,Jimenez L,Ait-Abderrahim H,Marchesseau S,Picart-Palmade Ldoi
10.1016/j.foodchem.2017.06.121subject
Has Abstractpub_date
2018-01-15 00:00:00pages
742-750eissn
0308-8146issn
1873-7072pii
S0308-8146(17)31103-2journal_volume
239pub_type
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