Characterisation and comparison of selected wheat (Triticum aestivum L.) cultivars and their blends to develop a gluten reference material.

Abstract:

:The reliability and comparability of gluten analytical results in gluten-free foods is hampered by the lack of reference materials (RM). This is partly caused by the yet incomplete knowledge of the effect of genetic and environmental variability of wheat proteins on immunochemical analyses, which affects the choice of gluten source to be applied for RM production. We investigated the genetic variability and the effect of harvest year on the protein composition of five previously selected wheat cultivars. We also compared the magnitude of these effects on ELISA results to get closer to the question of choosing individual cultivar or a mixture as an RM. Our results proved that the application of a blend for this purpose is advantageous. The candidates were also produced on pilot scale to investigate the feasibility of upscaling. The results of comparison studies showed that the pilot scale blended flour can also be suitable for RM.

journal_name

Food Chem

journal_title

Food chemistry

authors

Schall E,Scherf KA,Bugyi Z,Hajas L,Török K,Koehler P,Poms RE,D'Amico S,Schoenlechner R,Tömösközi S

doi

10.1016/j.foodchem.2019.126049

subject

Has Abstract

pub_date

2020-05-30 00:00:00

pages

126049

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(19)32195-8

journal_volume

313

pub_type

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