Abstract：
:The seeds of grass pea (Lathyrus sativus L.), a drought tolerant crop, were analysed for quantitative determination of the free amino acids β-N-oxalyl-l-α,β-diaminopropionic acid (β-ODAP), homoarginine and asparagine by a simple and fast capillary electrophoretic method. In boric acid (80mM, pH 8.0) running buffer system, not only were α and β-ODAP successfully separated, but also an efficient sample stacking was achieved during hydrodynamic sample introduction. The validated method was used for quantification of β-ODAP, homoarginine and asparagine in seed extracts of 52 Lathyrus local landraces from various regions of Turkey and one released cultivar. The concentration ranges of amino acids were found as 0.21-1.27% (w/w) for homoarginine, 0.10-0.87% (w/w) for β-ODAP and 0.006-0.47% (w/w) for asparagine. A positive correlation between homoarginine and β-ODAP quantities in seeds of 53 Lathyrus local landraces was shown to exist (r(2)=0.649).
journal_name
Food Chemjournal_title
Food chemistryauthors
Onar AN,Erdoğan BY,Ayan I,Acar Zdoi
10.1016/j.foodchem.2013.07.051subject
Has Abstractpub_date
2014-01-15 00:00:00pages
277-81eissn
0308-8146issn
1873-7072pii
S0308-8146(13)00979-5journal_volume
143pub_type
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