Abstract：
:Colourimetric determinations of amylase activity were developed based on a standard dinitrosalicylic acid (DNS) staining method, using maltose as the analyte. Intensities and absorbances of red, green and blue (RGB) were obtained with iPhone imaging and Adobe Photoshop image analysis. Correlation of green and analyte concentrations was highly significant, and the accuracy of the developed method was excellent in analytical performance. The common iPhone has sufficient imaging ability for accurate quantification of maltose concentrations. Detection limits, sensitivity and linearity were comparable to a spectrophotometric method, but provided better inter-day precision. In quantifying amylase specific activity from a commercial source (P>0.02) and fish samples (P>0.05), differences compared with spectrophotometric measurements were not significant. We have demonstrated that iPhone imaging with image analysis in Adobe Photoshop has potential for field and laboratory studies of amylase.
journal_name
Food Chemjournal_title
Food chemistryauthors
Thongprajukaew K,Choodum A,Sa-E B,Hayee Udoi
10.1016/j.foodchem.2014.04.080subject
Has Abstractpub_date
2014-11-15 00:00:00pages
87-91eissn
0308-8146issn
1873-7072pii
S0308-8146(14)00637-2journal_volume
163pub_type
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