Abstract：
:Well-run screening programs for cervical cancer in the population at risk have been shown to result in a sharp decrease in the incidence and mortality of cervical cancer in a number of large populations. Expression patterns of a recently identified biomarker family, microRNA, appear to be characteristic of tumor type and developmental origin. Several tumors have been reported to actively release exosomes carrying microRNAs. The present study has determined the association of microRNAs with cervical cancer-derived exosomes. The cervical cancer-derived exosomes were enriched in the cervicovaginal lavages specimens and the abundance of exosomes and exosomal microRNAs was detected by electron microscopy, western blot analysis, RT-qPCR and microRNA target reporter vector. The microRNA-21 and microRNA-146a, which were up-regulated in cervical cancer patients, were associated with the high levels of cervical cancer-derived exosomes. In conclusion, we demonstrated the abundance of exosomes in the cervicovaginal lavage specimens of women with cervical cancer. Furthermore, our results indicated that abnormally high levels of microRNA-21 and microRNA-146a existed in the cervical cancer-derived exosomes and the two microRNAs were functional in 293T cells.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Liu J,Sun H,Wang X,Yu Q,Li S,Yu X,Gong Wdoi
10.3390/ijms15010758subject
Has Abstractpub_date
2014-01-08 00:00:00pages
758-73issue
1issn
1422-0067pii
ijms15010758journal_volume
15pub_type
杂志文章abstract：:A series of gold(I) complexes of the general composition [Au(naza)(PPh₃)] (1-8) was prepared and thoroughly characterized (e.g., electrospray ionization (ESI) mass spectrometry and multinuclear nuclear magnetic resonance (NMR) spectroscopy). The N1-deprotonated anions of 7-azaindole or its derivatives (naza) are coord...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17122084
更新日期：2016-12-11 00:00:00
abstract：:Stearoyl-CoA desaturase 1 (SCD1), an enzyme that is involved in the biosynthesis of monounsaturated fatty acids, induces the reprogramming of cardiomyocyte metabolism. Thyroid hormones (THs) activate both lipolysis and lipogenesis. Many genes that are involved in lipid metabolism, including Scd1, are regulated by THs....
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22010109
更新日期：2020-12-24 00:00:00
abstract：:Programmed cell death (PCD) is a crucial process required for the normal development and physiology of metazoans. The three major mechanisms that induce PCD are called type I (apoptosis), type II (autophagic cell death), and type III (necrotic cell death). Dysfunctional PCD leads to diseases such as cancer and neurode...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms15023145
更新日期：2014-02-21 00:00:00
abstract：:Extramammary Paget's disease (EMPD) is a rare skin cancer arising in the anogenital area. Most EMPD tumors remain dormant as in situ lesions, but the outcomes of patients with metastatic EMPD are poor because of the lack of effective systemic therapies. Nectin cell adhesion molecule 4 (NECTIN4) has attracted attention...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165891
更新日期：2020-08-16 00:00:00
abstract：:Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed p...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071791
更新日期：2019-04-11 00:00:00
abstract：:In view of major economic problems caused by viruses, the development of genetically resistant crops is critical for breeders but remains limited by the evolution of resistance-breaking virus mutants. During the plant breeding process, the introgression of traits from Crop Wild Relatives results in a dramatic change o...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,meta分析,评审
doi：10.3390/ijms19102856
更新日期：2018-09-20 00:00:00
abstract：:Interleukin-18 (IL-18) is a multi-functional immuno-mediator in the development and progression of many types of infectious and inflammatory diseases. In this study, we evaluated the contribution of IL-18 genotypes to renal cell carcinoma (RCC) in Taiwan via the genotyping of IL-18 -656 (A/C), -607 (A/C), and -137 (G/...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20071563
更新日期：2019-03-28 00:00:00
abstract：:Following herbivore attacks, plants modify a blend of volatiles organic compounds (VOCs) released, resulting in the attraction of their antagonists. However, volatiles released constitutively may affect herbivores and natural enemies' fitness too. In tomato there is still a lack of information on the genetic bases res...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19092748
更新日期：2018-09-13 00:00:00
abstract：:Neural diseases including injury by endogenous factors, traumatic brain injury, and degenerative neural injury are eventually due to reactive oxygen species (ROS). Thus ROS generation in neural tissues is a hallmark feature of numerous forms of neural diseases. Neural degeneration and the neural damage process is comp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms160819657
更新日期：2015-08-19 00:00:00
abstract：:In plants, microRNAs have been indicated to control various developmental processes, including somatic embryogenesis (SE), which is triggered in the in vitro cultured somatic cells of plants. Although a transcriptomic analysis has indicated that numerous MIRNAs are differentially expressed in the SE of different plant...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205221
更新日期：2019-10-21 00:00:00
abstract：:Spiciformin (1) is a sesquiterpene lactone with a germacrane skeleton that is found in two Tanacetum species endemic to the Canary Islands. In this study, the cytotoxicities of 1 and its acetyl derivative (2) were evaluated against human tumor cells. These sesquiterpene lactones were cytotoxic against human acute myel...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082782
更新日期：2020-04-16 00:00:00
abstract：:Immunosenescence defines the decline in immune function that occurs with aging. This has been associated, at least in part, with defective cellular signaling via protein kinase C (PKC) signal transduction pathways. Our data suggest reduced PKC activation and consequently reduced response to lipopolysaccharide (LPS) st...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18071453
更新日期：2017-07-06 00:00:00
abstract：:Nimodipine is well characterized for the management of aneurysmal subarachnoid hemorrhage and has been shown to promote a better outcome and less delayed ischemic neurological deficits. Animal and clinical trials show neuroprotective efficacy following nerve injuries. We showed a neuroprotective effect on Neuro2a cell...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18050964
更新日期：2017-05-03 00:00:00
abstract：:Multiple myeloma (MM)-induced bone disease occurs through hyperactivation of osteoclasts by several factors secreted by MM cells. MM cell-secreted factors induce osteoclast differentiation and activation via direct and indirect actions including enhanced expression of receptor activator of nuclear factor κB ligand (RA...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21217905
更新日期：2020-10-24 00:00:00
abstract：:Seed storability, defined as the ability to remain alive during storage, is an important agronomic and physiological characteristic, but the underlying genetic mechanism remains largely unclear. Here, we report quantitative trait loci (QTLs) analyses for seed storability using a high-density single nucleotide polymorp...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184442
更新日期：2019-09-09 00:00:00
abstract：:Plastoglobules (PGs) are thylakoid membrane microdomains within plastids that are known as specialized locations of carotenogenesis. Three rice phytoene synthase proteins (OsPSYs) involved in carotenoid biosynthesis have been identified. Here, the N-terminal 80-amino-acid portion of OsPSY2 (PTp) was demonstrated to be...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18010018
更新日期：2016-12-22 00:00:00
abstract：:The aim of this study is to elucidate the detailed mechanism of endoplasmic reticulum (ER) stress-induced auditory cell death based on the function of the initiator caspases and molecular complex of necroptosis. Here, we demonstrated that ER stress initiates not only caspase-9-dependent intrinsic apoptosis along with ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20235896
更新日期：2019-11-24 00:00:00
abstract：:Sunflower (Helianthus annuus L.) oil remains under-utilised albeit one of the major seed oils produced world-wide. Moreover, the high oleic sunflower varieties make the oil attractive for applications requiring high temperature processes and those targeting the C=C double bond functionality. Herein an overview of the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms9081393
更新日期：2008-08-01 00:00:00
abstract：:Kidney-yang deficiency syndrome (KYDS) is a metabolic disease caused by a neuro-endocrine disorder. The You-gui pill (YGP) is a classic traditional Chinese medicine (TCM) formula for the treatment of KYDS and has been widely used to warm and recuperate KYDS clinically for hundreds of years in China. However, it is unk...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20153655
更新日期：2019-07-26 00:00:00
abstract：:Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a group 1 carcinogen that introduces mutagenic DNA adducts into the genome. In this study, we investigated the molecular mechanisms underlying the involvement of silymarin in the reduction of DNA adduct formation by B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE)...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21072369
更新日期：2020-03-30 00:00:00
abstract：:Variegated plants are valuable materials for investigating leaf color regulated mechanisms. To unveil the role of posttranslational modification in the variegated phenotype, we conducted global quantitative phosphoproteomic analysis on different leaf color sectors of Maiyuanjinqiu and the corresponding of Catalpa farg...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20081895
更新日期：2019-04-17 00:00:00
abstract：:This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12021306
更新日期：2011-02-22 00:00:00
abstract：:This narrative review aims to summarize the current controversy on the balance between ethanol and phytochemicals in wine, focusing on light drinking and oral cancer. Extensive literature search included PUBMED and EMBASE databases to identify in human studies and systematic reviews (up to March 2015), which contribut...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms160817029
更新日期：2015-07-27 00:00:00
abstract：:In healthy individuals, the healing of soft tissues such as skin after pathological insult or post injury follows a relatively predictable and defined series of cell and molecular processes to restore tissue architecture and function(s). Healing progresses through the phases of hemostasis, inflammation, proliferation,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21031015
更新日期：2020-02-04 00:00:00
abstract：:The common carp can tolerate extremely low oxygen levels. These fish store zinc in a specific zinc-binding protein presented in digestive tract tissues, and under low oxygen, the stored zinc is released and used as a signal to stimulate erythropoiesis (red blood cell formation). To determine whether the environmental ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18010138
更新日期：2017-01-11 00:00:00
abstract：:In the past few years, thorough investigation of chemical modifications operated in the cells on ribonucleic acid (RNA) molecules is gaining momentum. This new field of research has been dubbed "epitranscriptomics", in analogy to best-known epigenomics, to stress the potential of ensembles of RNA modifications to cons...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21134684
更新日期：2020-06-30 00:00:00
abstract：:NASH is becoming increasingly common worldwide because of the growing global prevalence of obesity and consequently NAFLD. Unfortunately, the mechanism of progression of NAFLD to NASH and then cirrhosis is not completely understood. Several factors, including insulin resistance, inflammation, oxidative stress, lipotox...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19072034
更新日期：2018-07-13 00:00:00
abstract：:Kaempferol, a flavonoid compound, is derived from the rhizome of Kaempferia galanga L., which is used in traditional medicine in Asia. Autophagy has pleiotropic functions that are involved in cell growth, survival, nutrient supply under starvation, defense against pathogens, and antigen presentation. There are many st...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19010125
更新日期：2018-01-02 00:00:00
abstract：:Oxidative damages induced by a redox imbalance cause age-related changes in cells and tissues. Superoxide dismutase (SOD) enzymes play a major role in the antioxidant system and they also catalyze superoxide radicals (O2·-). Since the loss of cytoplasmic SOD (SOD1) resulted in aging-like phenotypes in several types of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140610998
更新日期：2013-05-24 00:00:00
abstract：:The micronutrients vitamins B9 and B12 act as methyl donors in the one-carbon metabolism involved in transmethylation reactions which critically influence epigenetic mechanisms and gene expression. Both vitamins are essential for proper development, and their deficiency during pregnancy has been associated with a wide...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20205097
更新日期：2019-10-14 00:00:00
