A new high-sensitive nephelometric method for assaying serum C-reactive protein based on phosphocholine interaction.

abstract：:It has been well reported over recent years that most errors within the total testing process occur in the pre-analytical phase (46%-68.2%), an area that is usually outside of the direct control of the laboratory and which includes sample collection (phlebotomy). National and international (WHO, CLSI) guidelines recom...

journal_title：Clinical chemistry and laboratory medicine

authors： Cornes M,van Dongen-Lases E,Grankvist K,Ibarz M,Kristensen G,Lippi G,Nybo M,Simundic AM,Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM).

更新日期：2017-01-01 00:00:00

abstract：:Method comparisons are indispensable tools for the extensive validation of analytic procedures. Laboratories often only want to know whether an established procedure (x-method) can be replaced by another one (y-method) without interfering with diagnostic purposes. Then split patients' samples are analyzed more or less...

journal_title：Clinical chemistry and laboratory medicine

authors： Haeckel R,Wosniok W,Al Shareef N

更新日期：2011-11-01 00:00:00

abstract：:In view of the role of pharmacotherapy in medicine, on the one hand, and the powerful technical possibilities that are now available on the other hand, therapeutic drug monitoring is a surprisingly neglected area of laboratory medicine. In this viewpoint article, an "omics approach" to pharmacovigilance and drug monit...

journal_title：Clinical chemistry and laboratory medicine

authors： Vogeser M

更新日期：2020-05-05 00:00:00

abstract：BACKGROUND:Point of care (POC) assays for cardiac troponins I or T (cTnI or cTnT) may accelerate the diagnosis of patients with suspected acute coronary syndrome (ACS). However, their clinical utility according to the 0 h/3 h algorithm recommended by the European Society of Cardiology (ESC) for non-ST elevation myocard...

journal_title：Clinical chemistry and laboratory medicine

authors： Suh D,Keller DI,Hof D,von Eckardstein A,Gawinecka J

更新日期：2018-03-28 00:00:00

abstract：BACKGROUND:Extensive application of measurement of urinary iodine concentration (UIC) in several benign and malignant thyroid diseases could profit by the availability of rapid and inexpensive measuring techniques. Aim of this study was to apply a simple and inexpensive commercially available potentiometric method for ...

journal_title：Clinical chemistry and laboratory medicine

authors： Giovacchini G,Giovanella L,Haldemann A,Staub U,Füchsel FG,Koch P

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND:CD4+CD25+ forkhead box P3 (FOXP3)+ regulatory T cells (Tregs) accumulate in malignant tumors and negatively regulate antitumor immunity. However, the clinical significance of Tregs in HCC remains unclear. To determine the prognostic value of Tregs, we conducted a retrospective study on 264 patients with Barc...

journal_title：Clinical chemistry and laboratory medicine

authors： Li F,Guo Z,Lizée G,Yu H,Wang H,Si T

更新日期：2014-09-01 00:00:00

abstract：:Experimental and observational studies support a role of plasma homocysteine levels (tHcy) in coronary artery disease (CAD). In the GENICA (Genetic and Environmental factors In Coronary Atherosclerosis) study, we found that high tHcy predicted cardiovascular mortality in hypertensive, but not in normotensive, patients...

journal_title：Clinical chemistry and laboratory medicine

authors： Rossi GP,Seccia TM,Pessina AC

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9, play an important role in tumor invasion and metastasis. This study aimed to determine the serum levels of MMP-2, MMP-9, 130- and 225-kDa gelatinolytic bands and conventional tumor markers, carcinoembryonic antigen (CEA) and cancer antigen (CA) 19...

journal_title：Clinical chemistry and laboratory medicine

authors： Emara M,Cheung PY,Grabowski K,Sawicki G,Wozniak M

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Potassium is an important serum ion that is frequently assayed in clinical laboratories. Quality assurance requires reference methods; thus, the establishment of a candidate reference method for serum potassium measurements is important. METHODS:An inductively coupled plasma mass spectrometry (ICP-MS) metho...

journal_title：Clinical chemistry and laboratory medicine

authors： Yan Y,Han B,Zeng J,Zhou W,Zhang T,Zhang J,Chen W,Zhang C

更新日期：2017-08-28 00:00:00

abstract：Objectives:Matrix differences among serum samples from non-pregnant and pregnant patients could bias measurements. Standard Reference Material 1949, Frozen Human Prenatal Serum, was developed to provide a quality assurance material for the measurement of hormones and nutritional elements throughout pregnancy. Methods:...

journal_title：Clinical chemistry and laboratory medicine

authors： Boggs ASP,Kilpatrick LE,Burdette CQ,Tevis DS,Fultz ZA,Nelson MA,Jarrett JM,Kemp JV,Singh RJ,Grebe SKG,Wise SA,Kassim BL,Long SE

更新日期：2020-10-26 00:00:00

abstract：BACKGROUND:Antinuclear autoantibody determination relies on an initial screening step using immunofluorescence on HEp2 cells. This may be followed by anti-deoxyribonucleic acid (DNA) determination, if the fluorescence of nuclei is homogeneous. We assessed the validity of a restricted algorithm and compared this to a mo...

journal_title：Clinical chemistry and laboratory medicine

authors： Servais G,Karmali R,Guillaume MP,Badot V,Duchateau J,Corazza F

更新日期：2009-01-01 00:00:00

abstract：PURPOSE:Mechanical manipulation of the prostate is a generally accepted interfering factor for the measurement of prostate-specific antigen (PSA). However, only few studies have focused on common daily mechanical manipulations, such as bicycle riding. Furthermore, physical exercise is also supposed to modulate PSA seru...

journal_title：Clinical chemistry and laboratory medicine

authors： Herrmann M,Scharhag J,Sand-Hill M,Kindermann W,Herrmann W

更新日期：2004-03-01 00:00:00

abstract：BACKGROUND:In analogy with other areas of laboratory diagnostics, the pre-analytical phase is the leading source of variability also in urinalysis. We carried out a multicentric study for comparing results obtained from first-voided and mid-stream urine samples. METHODS:Each of the six hospital-based clinical laborato...

journal_title：Clinical chemistry and laboratory medicine

authors： Manoni F,Gessoni G,Alessio MG,Caleffi A,Saccani G,Silvestri MG,Poz D,Ercolin M,Tinello A,Valverde S,Ottomano C,Lippi G

更新日期：2011-12-20 00:00:00

abstract：:Reduced and total homocysteine, cysteine, glutathione and cysteinylglycine in plasma were investigated in 19 patients with chronic obstructive pulmonary disease and in 29 healthy subjects. The purpose was to examine the influence of pro-oxidant activity caused by the lung disease on the metabolism of homocysteine and ...

journal_title：Clinical chemistry and laboratory medicine

authors： Andersson A,Ankerst J,Lindgren A,Larsson K,Hultberg B

更新日期：2001-03-01 00:00:00

abstract：BACKGROUND:Circulating trimethylamine-N-oxide (TMAO) has been implicated in the development of cardiovascular and chronic kidney diseases (CKD). However, while higher TMAO levels have been associated with increased risks of cardiovascular or renal events in first prospective studies, it remained unclear how much plasma...

journal_title：Clinical chemistry and laboratory medicine

authors： Kühn T,Rohrmann S,Sookthai D,Johnson T,Katzke V,Kaaks R,von Eckardstein A,Müller D

更新日期：2017-02-01 00:00:00

abstract：BACKGROUND:There is a certain difference in opinion regarding the optimal choice of housekeeping genes used as normalization factors in gene expression analysis. We have therefore examined the suitability of three housekeeping genes, hypoxanthine phosphoribosyl transferase, beta-glucuronidase and beta2-micro-globulin, ...

journal_title：Clinical chemistry and laboratory medicine

authors： Kågedal B,Farnebäck M,Håkansson A,Gustafsson B,Håkansson L

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Squamous cell carcinoma antigen-immunoglobulin M (SCCA-IgM) is a useful biomarker for the risk of development of hepatocellular carcinoma (HCC) in patients with cirrhosis due to its progressive increase associated to HCC evolution. In patients with cirrhosis, other assays have been affected by interfering re...

journal_title：Clinical chemistry and laboratory medicine

authors： Zuin J,Veggiani G,Pengo P,Gallotta A,Biasiolo A,Tono N,Gatta A,Pontisso P,Toth R,Cerin D,Frecer V,Meo S,Gion M,Fassina G,Beneduce L

更新日期：2010-02-01 00:00:00

abstract：BACKGROUND:Asymmetric dimethylarginine (ADMA), an endogenous competitive inhibitor of nitric oxide synthase plays an important role in endothelial dysfunction processes. Recent studies have linked high ADMA levels with several pathological conditions. The interest as a marker of endothelial dysfunction has increased in...

journal_title：Clinical chemistry and laboratory medicine

authors： Gervasoni J,Bonelli F,Zuppi C,Zappacosta B,Mordente A,Calvani R,Persichilli S

更新日期：2011-09-06 00:00:00

abstract：BACKGROUND:Cigarette smoking, a cardiovascular risk factor leading to oxygen free radical formation, is involved in the development of serious pathological conditions. On the other hand, a healthy diet and adequate supplementation can help prevent many diseases. The aim of our study was to evaluate in healthy light smo...

journal_title：Clinical chemistry and laboratory medicine

authors： Bamonti F,Novembrino C,Ippolito S,Soresi E,Ciani A,Lonati S,Scurati-Manzoni E,Cighetti G

更新日期：2006-01-01 00:00:00

abstract：:Abstract Lipoprotein-related risk of cardiovascular disease (CVD) can be adequately predicted in subjects with elevated total cholesterol and low-density lipoprotein (LDL-)cholesterol using the available guidelines. However, individuals with dyslipidemia can have normal total- and LDL-cholesterol concentrations. Many ...

journal_title：Clinical chemistry and laboratory medicine

authors： Langlois MR

更新日期：2012-04-10 00:00:00

abstract：BACKGROUND:Crohn's disease (CD) is an incurable and difficult to diagnose condition. While high sensitive C-reactive protein (CRP) remains the best biochemical marker, we evaluated the diagnostic usefulness of lipid peroxidation indices. METHODS:Malondialdehyde/thiobarbituric acid-reactive substances (MDA/TBARS), pero...

journal_title：Clinical chemistry and laboratory medicine

authors： Boehm D,Krzystek-Korpacka M,Neubauer K,Matusiewicz M,Paradowski L,Gamian A

更新日期：2012-03-03 00:00:00

abstract：BACKGROUND:The possible effects of the uncoupling protein-1 (UCP-1) gene -3826A/G polymorphism on hypertension (HT) have yet to be elucidated. METHODS:A total of 578 Japanese subjects (231 males and 347 females, mean age 58.4 years) were enrolled in the study to investigate the association between HT and the -3826A/G ...

journal_title：Clinical chemistry and laboratory medicine

authors： Kotani K,Sakane N,Saiga K,Tsuzaki K,Shimohiro H,Tabata M,Kurozawa Y

更新日期：2007-01-01 00:00:00

abstract：:Phenylketonuria is an inherited metabolic disease, which is characterized by an increased level of serum phenylalanine. Quantitative measurement of phenylalanine in the serum of phenylketonuria patients is necessary to confirm the disease, and to distinguish phenylketonuria from other forms of hyperphenylalaninemia. I...

journal_title：Clinical chemistry and laboratory medicine

authors： Vallian S,Moeini H

更新日期：2006-01-01 00:00:00

abstract：:Immunological reactions are influenced by various factors including antigens, antibodies and other variables. We focused on two items: i) matrix effects, especially of detergents and ii) temperature effects: preheating sera, especially effects on rheumatoid factor (RF) measurement and false-positive reactions in ELISA...

journal_title：Clinical chemistry and laboratory medicine

authors： Yoshida H,Imafuku Y,Nagai T

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:The objective of the present study was to calculate age- and gender-specific reference ranges for serum insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein 3 (IGFBP-3) concentrations using quantile regression. METHODS:From the Study of Health in Pomerania (SHIP), 1798 men and...

journal_title：Clinical chemistry and laboratory medicine

authors： Friedrich N,Krebs A,Nauck M,Wallaschofski H

更新日期：2010-01-01 00:00:00

abstract：:Objectives Our recent survey of 44 mass spectrometry laboratories across 17 countries identified variation in internal standard (IS) choice for the measurement of serum/plasma 17α-hydroxyprogesterone (17OHP) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The choice of IS may contribute to inter-method v...

journal_title：Clinical chemistry and laboratory medicine

authors： Loh TP,Ho CS,Hartmann MF,Zakaria R,Lo CWS,van den Berg S,de Rijke YB,Cooke BR,Hoad K,Graham P,Davies SR,Mackay LG,Wudy SA,Greaves RF

更新日期：2020-09-25 00:00:00

abstract：:The history of prealbumin dates back to the early forties and may be divided into three parts, based on a chronological and functional approach. The first part--the discovery and the identification of prealbumin--was essentially based on classical protein chemistry methods. The second--the demonstration of prealbumin ...

journal_title：Clinical chemistry and laboratory medicine

authors： De Nayer P

更新日期：2002-12-01 00:00:00

abstract：:In the relatively short time frame since the introduction of next generation sequencing, it has become a method of choice for complex genomic research studies. As a paradigm shifting technology, we are now witnessing its translation into clinical diagnostic laboratories for patient care. Multi-gene panels for a variet...

journal_title：Clinical chemistry and laboratory medicine

authors： Coonrod EM,Margraf RL,Voelkerding KV

更新日期：2011-12-23 00:00:00

abstract：:Background Quantification of plasma amino acids is key to the diagnosis of inherited defects of amino acid synthesis, catabolism and transport, many of which present as clinical emergencies. The utility of this test is limited by the long analysis time and subsequent inability of laboratories to provide results in rea...

journal_title：Clinical chemistry and laboratory medicine

authors： Carling RS,John K,Churchus R,Turner C,Dalton RN

更新日期：2020-04-28 00:00:00

abstract：:Reference intervals in clinical chemistry are commonly based on results of measurements in reference populations or are taken from the literature. A reference population should represent a defined group of individuals and be as similar as possible to the patients under investigation. Frequently, reference populations ...

journal_title：Clinical chemistry and laboratory medicine

authors： Kallner A,Gustavsson E,Hendig E

更新日期：2000-07-01 00:00:00