Expression of enzymatically active reverse transcriptase in Escherichia coli.

abstract：:High-resolution magnetic resonance microscopy (MRM) was used to determine regional brain volumetric changes in a mouse model of Alzheimer's disease. These transgenic (Tg) mice overexpress human mutant amyloid precursor protein (APP) V717F under control of platelet-derived growth factor promoter (PDAPP mice), and corti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Redwine JM,Kosofsky B,Jacobs RE,Games D,Reilly JF,Morrison JH,Young WG,Bloom FE

更新日期：2003-02-04 00:00:00

abstract：:Hydrogen/deuterium exchange followed by trapping of the labeled species in the aprotic solvent DMSO has been used to elucidate structure in both the burst-phase molten globule-folding intermediate of apomyoglobin and in an equilibrium intermediate that models the kinetic intermediate. Precise estimates can be made of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishimura C,Dyson HJ,Wright PE

更新日期：2005-03-29 00:00:00

abstract：:Corticosteroid hormones are critical for controlling metabolism, hydromineral balance, and the stress response in vertebrates. Although corticosteroid hormones have been well characterized in most vertebrate groups, the identity of the earliest vertebrate corticosteroid hormone has remained elusive. Here we provide ev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Close DA,Yun SS,McCormick SD,Wildbill AJ,Li W

更新日期：2010-08-03 00:00:00

abstract：:We have shown previously that guanine nucleotide-binding protein (G protein) beta gamma complexes purified from bovine brain membranes are methyl esterified on a C-terminal cysteine residue of the gamma polypeptide. In the present study, 3H-methylated G beta gamma complexes cleaved to their constituent amino acids by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamane HK,Farnsworth CC,Xie HY,Howald W,Fung BK,Clarke S,Gelb MH,Glomset JA

更新日期：1990-08-01 00:00:00

abstract：:The selection of a single molecular handedness, or homochirality across all living matter, is a mystery in the origin of life. Frank's seminal model showed in the '50s how chiral symmetry breaking can occur in nonequilibrium chemical networks. However, an important shortcoming in this classic model is that it consider...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laurent G,Lacoste D,Gaspard P

更新日期：2021-01-19 00:00:00

abstract：:We used a high-titer recombinant adeno-associated virus (rAAV) vector to express WT or mutant human alpha-synuclein in the substantia nigra of adult marmosets. The alpha-synuclein protein was expressed in 90-95% of all nigral dopamine neurons and distributed by anterograde transport throughout their axonal and dendrit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirik D,Annett LE,Burger C,Muzyczka N,Mandel RJ,Björklund A

更新日期：2003-03-04 00:00:00

abstract：:The neutrality theory predicts that the rate of neutral molecular evolution is constant over time, and thus that there is a molecular clock for timing evolutionary events. It has been observed that the variance of the rate of evolution is generally larger than expected according to the neutrality theory, which has rai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rodríguez-Trelles F,Tarrío R,Ayala FJ

更新日期：2001-09-25 00:00:00

abstract：:We report the development of a practical ultrafast allelic profiling assay for the analysis of short tandem repeats (STRs) by using a highly optimized microfluidic electrophoresis device. We have achieved baseline-resolved electrophoretic separations of single-locus STR samples in 30 sec. Analyses of PCR samples conta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmalzing D,Koutny L,Adourian A,Belgrader P,Matsudaira P,Ehrlich D

更新日期：1997-09-16 00:00:00

abstract：:The effect of metabolic depletion on the maturation-associated loss of membrane functions has been studied by using sheep reticulocytes incubated in vitro at 37 degrees C for periods up to 41 hr. ATP was either maintained with glucose, adenosine plus inosine, or depleted with 2-deoxyglucose plus arsenate. Two membrane...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weigensberg AM,Blostein R

更新日期：1983-08-01 00:00:00

abstract：:Papain solubilized H-2a histocompatibility antigens (H-2Kk plus H-2Dd) have been purified by a large-scale procedure that can routinely provide 2-3 mg of heavy chain from 1 kg of mouse liver. The heavy chains were homogeneous by sodium dodecyl sulfate electrophoresis. Disc gel electrophoresis resolved two protein band...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henriksen O,Robinson EA,Appella E

更新日期：1978-07-01 00:00:00

abstract：:The formation of viable genetic chimeras in mammals through the transfer of cells between siblings in utero is rare. Using microsatellite DNA markers, we show here that chimerism in marmoset (Callithrix kuhlii) twins is not limited to blood-derived hematopoietic tissues as was previously described. All somatic tissue ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ross CN,French JA,Ortí G

更新日期：2007-04-10 00:00:00

abstract：:The molecular chaperone Hsp90 is a protein folding machine that is conserved from bacteria to man. Human, cytosolic Hsp90 is dedicated to folding of chiefly signal transduction components. The chaperoning mechanism of Hsp90 is controlled by ATP and various cochaperones, but is poorly understood and controversial. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karagöz GE,Duarte AM,Ippel H,Uetrecht C,Sinnige T,van Rosmalen M,Hausmann J,Heck AJ,Boelens R,Rüdiger SG

更新日期：2011-01-11 00:00:00

abstract：:Here, the ATP-binding, ATP hydrolysis, mispair-binding, sliding clamp formation, and Mlh1-Pms1 complex interaction properties of dominant mutant Msh2-Msh6 complexes have been characterized. The results demonstrate two mechanisms for dominance. In one, seen with the Msh6-S1036P and Msh6-G1067D mutant complexes, the mut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hess MT,Mendillo ML,Mazur DJ,Kolodner RD

更新日期：2006-01-17 00:00:00

abstract：:Let T(k) (epsilon)(lambda; t(1),..., t(k)) = rho(X(t) (1))q(epsilon)(X(t) (2) - X(t) (1))... q(epsilon)(X(t(k) ) - X(t(k) )-1), where X(t) is a Brownian motion in R(2), lambda(dx) = rho(x)dx, and q(epsilon) converges to Dirac's delta function as epsilon downward arrow 0. The self-intersection local times of order k ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dynkin EB

更新日期：1986-06-01 00:00:00

abstract：:Aptamers recognize their targets with extraordinary affinity and specificity. The aptamer-based therapeutic, Macugen, is derived from a modified 2'fluoro pyrimidine RNA inhibitor to vascular endothelial growth factor (VEGF) and is now being used to treat the wet form of age-related macular degeneration. This VEGF(165)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee JH,Canny MD,De Erkenez A,Krilleke D,Ng YS,Shima DT,Pardi A,Jucker F

更新日期：2005-12-27 00:00:00

abstract：:The world's oceans are undergoing profound changes as a result of human activities. However, the consequences of escalating human impacts on marine mammal biodiversity remain poorly understood. The International Union for the Conservation of Nature (IUCN) identifies 25% of marine mammals as at risk of extinction, but ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davidson AD,Boyer AG,Kim H,Pompa-Mansilla S,Hamilton MJ,Costa DP,Ceballos G,Brown JH

更新日期：2012-02-28 00:00:00

abstract：:Deuterium Fourier-transform nuclear magnetic resonance spectra have been obtained of 1-myristoyl 2-(14,14,14-trideutero)myristoyl phosphatidylcholine bilayers at 34.1 MHz by using the quadrupole echo pulse technique. Thereby, we have investigated the effects upon the deuterated dimyristoyl phosphatidylcholine bilayers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oldfield E,Gilmore R,Glaser M,Gutowsky HS,Hshung JC,Kang SY,King TE,Meadows M,Rice D

更新日期：1978-10-01 00:00:00

abstract：:Dietary diversity often varies inversely with prey resource abundance. This pattern, although typically measured at the population level, is usually assumed to also characterize the behavior of individual animals within the population. However, the pattern might also be produced by changes in the degree of variation a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tinker MT,Bentall G,Estes JA

更新日期：2008-01-15 00:00:00

abstract：:Low phosphate (Pi) availability is one of the major constraints for plant productivity in natural and agricultural ecosystems. Plants have evolved a myriad of developmental and biochemical mechanisms to increase internal Pi uptake and utilization efficiency. One important biochemical pathway leading to an increase in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cruz-Ramírez A,Oropeza-Aburto A,Razo-Hernández F,Ramírez-Chávez E,Herrera-Estrella L

更新日期：2006-04-25 00:00:00

abstract：:Development of effective chemopreventive agents against prostate cancer (CaP) for humans requires conclusive evidence of their efficacy in animal models that closely emulates human disease. The autochthonous transgenic adenocarcinoma of the mouse prostate (TRAMP) model, which spontaneously develops metastatic CaP, is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gupta S,Hastak K,Ahmad N,Lewin JS,Mukhtar H

更新日期：2001-08-28 00:00:00

abstract：:A set of at least 30 proteins disappears from the two-dimensional electrophoretic pattern of human lymphoid cells treated with various antimitochondrial agents. This set is similar to the set of proteins found in isolated mitochondria (except for the presence of action in the latter group), indicating that the inhibit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson L

更新日期：1981-04-01 00:00:00

abstract：:Four different human cell lines transformed by simian virus 40 (SV40) were tested for their tumorigenicity in athymic nude mice. Two of these lines, W18Va2 and GM52VA, were found to be tumorigenic when inoculated at a concentration of 1 x 10(7) cells per mouse. The other two cell lines, LN-SV and GM54VA, were found to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koprowski H,Croce CM

更新日期：1977-03-01 00:00:00

abstract：:DNA from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (HPV) type 11 DNA only under nonstringent conditions. This DNA was molecularly cloned in lambda phage. Under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dürst M,Gissmann L,Ikenberg H,zur Hausen H

更新日期：1983-06-01 00:00:00

abstract：:We modeled the behavior of recessive mutations with deleterious effects to either the sporophyte or the gametophyte, or both, in polysomic tetraploid populations by allowing for varying levels of double reduction, mutation, and self-fertilization. Double reduction causes a decrease of the equilibrium frequencies of de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butruille DV,Boiteux LS

更新日期：2000-06-06 00:00:00

abstract：:P2X receptors for ATP have a wide range of physiological roles and comprise a structurally distinct family of ligand-gated trimeric ion channels. The crystal structure of a P2X4 receptor, in combination with mutagenesis studies, has provided a model of the intersubunit ATP-binding sites and identified an extracellular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts JA,Allsopp RC,El Ajouz S,Vial C,Schmid R,Young MT,Evans RJ

更新日期：2012-03-20 00:00:00

abstract：:Specific antibodies against types I and III collagens and procollagens were used to localize these proteins in cultured human cells. These studies indicate that the same cell makes both proteins. No type III procollagen synthesis was observed in cells from two patients with two patients with the Ehlers-Danlos type IV ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gay S,Martin GR,Muller PK,Timpl R,Kuhn K

更新日期：1976-11-01 00:00:00

abstract：:In characterizing mice with targeted disruption of the SerpinB2 gene, we observed animals that were small at birth with delayed growth and decreased life expectancy. Although this phenotype cosegregated with homozygosity for the inactive SerpinB2 allele, analysis of homozygous SerpinB2-deficient mice derived from two ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Westrick RJ,Mohlke KL,Korepta LM,Yang AY,Zhu G,Manning SL,Winn ME,Dougherty KM,Ginsburg D

更新日期：2010-09-28 00:00:00

abstract：:A distinct phosphodiesterasic activity (EC 3.1.4) was found in both mono- and dicotyledonous plants that catalyzes the hydrolytic breakdown of ADPglucose (ADPG) to produce equimolar amounts of glucose-1-phosphate and AMP. The enzyme responsible for this activity, referred to as ADPG pyrophosphatase (AGPPase), was puri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rodriguez-López M,Baroja-Fernández E,Zandueta-Criado A,Pozueta-Romero J

更新日期：2000-07-18 00:00:00

abstract：:Local levels of active thyroid hormone (3,3',5-triiodothyronine) are controlled by the action of activating and inactivating iodothyronine deiodinase enzymes. Deiodinases are selenocysteine-dependent membrane proteins catalyzing the reductive elimination of iodide from iodothyronines through a poorly understood mechan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schweizer U,Schlicker C,Braun D,Köhrle J,Steegborn C

更新日期：2014-07-22 00:00:00

abstract：:Two-component histidine kinases recently have been found in eukaryotic organisms including fungi, slime molds, and plants. We describe the identification of a gene, COS1, from the opportunistic pathogen Candida albicans by using a PCR-based screening strategy. The sequence of COS1 indicates that it encodes a homolog o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alex LA,Korch C,Selitrennikoff CP,Simon MI

更新日期：1998-06-09 00:00:00