Crystal structure of mammalian selenocysteine-dependent iodothyronine deiodinase suggests a peroxiredoxin-like catalytic mechanism.

abstract：:Micrometeorites with diameter approximately 100-200 microm dominate the flux of extraterrestrial matter on Earth. The vast majority of micrometeorites are chemically, mineralogically, and isotopically related to carbonaceous chondrites, which amount to only 2.5% of meteorite falls. Here, we report the discovery of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gounelle M,Chaussidon M,Morbidelli A,Barrat JA,Engrand C,Zolensky ME,McKeegan KD

更新日期：2009-04-28 00:00:00

abstract：:A complex between soluble dextran and human hemoglobin has been synthesized by two different methods. In the alkylation method, hemoglobin was allowed to react with bromoacetyl groups incorporated into the dextran; the yield of the complex was about 80% in terms of the hemoglobin used. In the dialdehyde method, hemogl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tam SC,Blumenstein J,Wong JT

更新日期：1976-06-01 00:00:00

abstract：:By manufacturing a single-particle system in two particulate forms (i.e., micrometer size and nanometer size), we have designed a bacterial vaccine form that exhibits improved efficacy of immunization. Microstructural properties are adapted to alter dispersive and aerosol properties independently. Dried "nanomicropart...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garcia-Contreras L,Wong YL,Muttil P,Padilla D,Sadoff J,Derousse J,Germishuizen WA,Goonesekera S,Elbert K,Bloom BR,Miller R,Fourie PB,Hickey A,Edwards D

更新日期：2008-03-25 00:00:00

abstract：:Isolated rat kidney glomeruli converted octatritiated arachidonic acid to several prostaglandins whose production was inhibited by meclofenamate. These were, in order of decreasing abundance, prostaglandin F2 alpha, prostaglandin E2, 6-oxo-prostaglandin F1 alpha, thromboxane B2, and prostaglandin D2. These products we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hassid A,Konieczkowski M,Dunn MJ

更新日期：1979-03-01 00:00:00

abstract：:In this report we identify the specific isozymes of protein kinase C (PKC) that are involved in c-fos and c-jun mRNA accumulation in the rat basophilic leukemia cell line RBL-2H3. These cells could be largely depleted of the endogenous PKC isozymes by chronic treatment with phorbol 12-myristate 13-acetate followed by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razin E,Szallasi Z,Kazanietz MG,Blumberg PM,Rivera J

更新日期：1994-08-02 00:00:00

abstract：:The role of Notch signaling in cartilage differentiation and maturation in vivo was examined. Conditional Notch pathway gain and loss of function was achieved using a Cre/loxP approach to manipulate Notch signaling in cartilage precursors and chondrocytes of the developing mouse embryo. Conditional overexpression of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mead TJ,Yutzey KE

更新日期：2009-08-25 00:00:00

abstract：:The sequence of oligonucleotides produced by the action of bleomycin and ferrous ion on double- and single-stranded DNA has been determined. In the presence of ferrous ion, bleomycin promotes cleavage at G-T and G-C sequences, while high concentrations of ferrous ion alone result in strand scission that is not base sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takeshita M,Grollman AP,Ohtsubo E,Ohtsubo H

更新日期：1978-12-01 00:00:00

abstract：:A detailed conformational analysis of the known crystal structures of vitamin A, carotenoid, and xanthenoid derivatives has been made. These studies suggest that the visual chromophore 11-cis retinal and its isomers may each occur in several different conformational states, which would be expected to have different li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sundaralingam M,Beddell C

更新日期：1972-06-01 00:00:00

abstract：:L-Fucose and D-galactose in low concentrations (0.27 or 2.7 mM) inhibited the induction of active Na+ channels in mouse and human neuroblastoma cells when the monosaccharides were added to the culture medium for 4 days with the inducing agent dimethyl sulfoxide. Active Na+ ionophores were determined by measurement of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giovanni MY,Kessel D,Glick MC

更新日期：1981-02-01 00:00:00

abstract：:Branching morphogenesis in the mammary gland is achieved by the migration of epithelial cells through a microenvironment consisting of stromal cells and extracellular matrix (ECM). Here we show that galectin-1 (Gal-1), an endogenous lectin that recognizes glycans bearing N-acetyllactosamine (LacNAc) epitopes, induces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat R,Belardi B,Mori H,Kuo P,Tam A,Hines WC,Le QT,Bertozzi CR,Bissell MJ

更新日期：2016-08-16 00:00:00

abstract：:The phosphatidylinositol 3-kinase subunit PIK3CA is frequently mutated in human cancers. Here we used gene targeting to "knock in" PIK3CA mutations into human breast epithelial cells to identify new therapeutic targets associated with oncogenic PIK3CA. Mutant PIK3CA knockin cells were capable of epidermal growth facto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gustin JP,Karakas B,Weiss MB,Abukhdeir AM,Lauring J,Garay JP,Cosgrove D,Tamaki A,Konishi H,Konishi Y,Mohseni M,Wang G,Rosen DM,Denmeade SR,Higgins MJ,Vitolo MI,Bachman KE,Park BH

更新日期：2009-02-24 00:00:00

abstract：:A panel of rat monoclonal antibodies was used to classify mouse mast cells from four different sources on the basis of their surface determinants. Three populations of mast cells were derived by culturing normal bone marrow, spleen, and peripheral blood cells, whereas the fourth population was isolated directly from t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Katz HR,LeBlanc PA,Russell SW

更新日期：1983-10-01 00:00:00

abstract：:In eukaryotes, transfer RNAs (tRNAs) are transcribed in the nucleus yet function in the cytoplasm; thus, tRNA movement within the cell was believed to be unidirectional--from the nucleus to the cytoplasm. It is now known that mature tRNAs also move in a retrograde direction from the cytoplasm to the nucleus via retrog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kramer EB,Hopper AK

更新日期：2013-12-24 00:00:00

abstract：:Understanding how changes in antibiotic consumption affect the prevalence of antibiotic resistance in bacterial pathogens is important for public health. In a number of bacterial species, including Streptococcus pneumoniae, the prevalence of resistance has remained relatively stable despite prolonged selection pressur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehtinen S,Blanquart F,Croucher NJ,Turner P,Lipsitch M,Fraser C

更新日期：2017-01-31 00:00:00

abstract：:MscS is a mechanosensitive channel that is ubiquitous among bacteria. Recent progress in the genome projects has revealed that homologs of MscS are also present in eukaryotes, but whether they operate as ion channels is unknown. In this study we cloned MSC1, a homolog of MscS in Chlamydomonas, and examined its functio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakayama Y,Fujiu K,Sokabe M,Yoshimura K

更新日期：2007-04-03 00:00:00

abstract：:Protein kinase C (PKC) phosphorylation stimulates the cystic fibrosis transmembrane conductance regulator (CFTR) channel and enhances its activation by protein kinase A (PKA) through mechanisms that remain poorly understood. We have examined the effects of mutating consensus sequences for PKC phosphorylation and repor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chappe V,Hinkson DA,Howell LD,Evagelidis A,Liao J,Chang XB,Riordan JR,Hanrahan JW

更新日期：2004-01-06 00:00:00

abstract：:The fetal genome regulates maternal physiology and behavior via its placenta, which produces hormones that act on the maternal hypothalamus. At the same time, the fetus itself develops a hypothalamus. In this study we show that many of the genes that regulate placental development also regulate the developing hypothal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Broad KD,Keverne EB

更新日期：2011-09-13 00:00:00

abstract：:Most genetic regulatory mechanisms involve protein-DNA interactions. In these processes, the classical Watson-Crick DNA structure sometimes is distorted severely, which in turn enables the precise recognition of the specific sites by the protein. Despite its key importance, very little is known about such deformation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leger JF,Robert J,Bourdieu L,Chatenay D,Marko JF

更新日期：1998-10-13 00:00:00

abstract：:Translesion synthesis at replication-blocking lesions requires the induction of proteins that are controlled by the SOS system in Escherichia coli. Of the proteins identified so far, UmuD', UmuC, and RecA* were shown to facilitate replication across UV-light-induced lesions, yielding both error-free and mutagenic tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuchs RP,Napolitano RL

更新日期：1998-10-27 00:00:00

abstract：:Transcriptional repressors can be classified as short- or long-range, according to their range of activity. Functional analysis of identified short-range repressors has been carried out largely in transgenic Drosophila, but it is not known whether general properties of short-range repressors are evident in other types...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ryu JR,Olson LK,Arnosti DN

更新日期：2001-11-06 00:00:00

abstract：:Interaction of syntaxin 1 with the alpha(1D) subunit of the voltage-gated L type Ca(2+) channel was investigated in the pancreatic beta cell. Coexpression of the enhanced green fluorescent protein-linked alpha(1D) subunit with the enhanced blue fluorescent protein-linked syntaxin 1 and Western blot analysis together w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang SN,Larsson O,Bränström R,Bertorello AM,Leibiger B,Leibiger IB,Moede T,Köhler M,Meister B,Berggren PO

更新日期：1999-08-31 00:00:00

abstract：:Skeletal muscle adapts to different patterns of motor nerve activity by alterations in gene expression that match specialized properties of contraction, metabolism, and muscle mass to changing work demands (muscle plasticity). Calcineurin, a calcium/calmodulin-dependent, serine-threonine protein phosphatase, has been ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenberg P,Hawkins A,Stiber J,Shelton JM,Hutcheson K,Bassel-Duby R,Shin DM,Yan Z,Williams RS

更新日期：2004-06-22 00:00:00

abstract：:The fluorescence decay of the rare earth terbium when bound to the protein calmodulin changes from a simple exponential decay to a complex nonexponential decay as the temperature is lowered below 200 K. We have fit the observed decay curves by assuming that the terbium emission is a forced electric dipole transition a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Austin RH,Stein DL,Wang J

更新日期：1987-03-01 00:00:00

abstract：:Peripheral blood mononuclear cells of 10 out of 26 patients with human immunodeficiency virus type 1 (HIV-1) released IgE-binding factors, as determined by two independent assays. The formation of the factors by the mononuclear cells was enhanced by incubation of the cells with homologous IgE. In the presence of IgE, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carini C,Margolick J,Yodoi J,Ishizaka K

更新日期：1988-12-01 00:00:00

abstract：:Zachariae Isstrøm (ZI) and Nioghalvfjerdsfjorden (79N) are marine-terminating glaciers in northeast Greenland that hold an ice volume equivalent to a 1.1-m global sea level rise. ZI lost its floating ice shelf, sped up, retreated at 650 m/y, and experienced a 5-gigaton/y mass loss. Glacier 79N has been more stable des...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： An L,Rignot E,Wood M,Willis JK,Mouginot J,Khan SA

更新日期：2021-01-12 00:00:00

abstract：:The T-cell receptor (TCR) gamma gene product occurs in association with T3 (CD3) polypeptides on the surface of human T lymphocytes. TCR gamma lymphocytes express arrays of T3 polypeptides distinct from those typically observed on TCR alpha beta lymphocytes. This report demonstrates that identical T3 gamma, delta, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krangel MS,Bierer BE,Devlin P,Clabby M,Strominger JL,McLean J,Brenner MB

更新日期：1987-06-01 00:00:00

abstract：:Mutualisms between fungi and fungus-growing animals are model systems for studying coevolution and complex interactions between species. Fungal growing behavior has enabled cultivating animals to rise to major ecological importance, but evolution of farming symbioses is thought to be restricted to three terrestrial in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silliman BR,Newell SY

更新日期：2003-12-23 00:00:00

abstract：:We developed two solid-phase reagent systems for microscopic visualization and characterization of melanocyte-stimulating hormone (MSH) receptors of melanoma cells. Multiple copies of [Nle-4,D-Phe-7]-alpha-MSH, a potent analog of alpha-MSH, were conjugated to microspheres (latex beads) or macrospheres (polyamide beads...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharma SD,Jiang J,Hadley ME,Bentley DL,Hruby VJ

更新日期：1996-11-26 00:00:00

abstract：:Conversion of carbon dioxide into hydrocarbons using solar energy is an attractive strategy for storing such a renewable source of energy into the form of chemical energy (a fuel). This can be achieved in a system coupling a photovoltaic (PV) cell to an electrochemical cell (EC) for CO2 reduction. To be beneficial and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huan TN,Dalla Corte DA,Lamaison S,Karapinar D,Lutz L,Menguy N,Foldyna M,Turren-Cruz SH,Hagfeldt A,Bella F,Fontecave M,Mougel V

更新日期：2019-05-14 00:00:00

abstract：:We have investigated the folding dynamics of Thermus thermophilus cytochrome c(552) by time-resolved fluorescence energy transfer between the heme and each of seven site-specific fluorescent probes. We have found both an equilibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada S,Bouley Ford ND,Keller GE,Ford WC,Gray HB,Winkler JR

更新日期：2013-01-29 00:00:00