Biodegradation-inspired bioproduction of methylacetoin and 2-methyl-2,3-butanediol.

Abstract:

:Methylacetoin (3-hydroxy-3-methylbutan-2-one) and 2-methyl-2,3-butanediol are currently obtained exclusively via chemical synthesis. Here, we report, to the best of our knowledge, the first alternative route, using engineered Escherichia coli. The biological synthesis of methylacetoin was first accomplished by reversing its biodegradation, which involved modifying the enzyme complex involved, switching the reaction substrate, and coupling the process to an exothermic reaction. 2-Methyl-2,3-butanediol was then obtained by reducing methylacetoin by exploiting the substrate promiscuity of acetoin reductase. A complete biosynthetic pathway from renewable glucose and acetone was then established and optimized via in vivo enzyme screening and host metabolic engineering, which led to titers of 3.4 and 3.2 g l(-1) for methylacetoin and 2-methyl-2,3-butanediol, respectively. This work presents a biodegradation-inspired approach to creating new biosynthetic pathways for small molecules with no available natural biosynthetic pathway.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Jiang X,Zhang H,Yang J,Zheng Y,Feng D,Liu W,Xu X,Cao Y,Zou H,Zhang R,Cheng T,Jiao F,Xian M

doi

10.1038/srep02445

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

2445

issn

2045-2322

pii

srep02445

journal_volume

3

pub_type

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