Abstract：
:Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (<10 ns) emission signals from organic chromophores or tissue autofluorescence. Here using a conventional animal imaging system not optimized for such long-lived excited states, we demonstrate improvement of signal to background contrast ratio by >50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Gu L,Hall DJ,Qin Z,Anglin E,Joo J,Mooney DJ,Howell SB,Sailor MJdoi
10.1038/ncomms3326subject
Has Abstractpub_date
2013-01-01 00:00:00pages
2326issn
2041-1723pii
ncomms3326journal_volume
4pub_type
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