Molecular diagnosis for indeterminate thyroid nodules on fine needle aspiration: advances and limitations.

abstract：:Developing more precise diagnostics approaches to predict cancer progression and prognosis is the key to precision medicine. Overwhelming evidence now suggests that small non-coding RNAs such as miRNAs can be useful tools as biomarkers for molecular diagnostics. miRNAs can serve as biomarkers in a variety of diseases,...

journal_title：Expert review of molecular diagnostics

authors： Mishra PJ

更新日期：2014-11-01 00:00:00

abstract：INTRODUCTION:The role of copy number variants (CNVs) in disease is now well established. In parallel NGS technologies, such as long-read technologies, there is continual development and data analysis methods continue to be refined. Clinical exome sequencing data is now a reality for many diagnostic laboratories in both...

journal_title：Expert review of molecular diagnostics

authors： Hehir-Kwa JY,Tops BBJ,Kemmeren P

更新日期：2018-10-01 00:00:00

abstract：:There is great interest in the development of noninvasive methods for imaging angiogenic responses. Strategies for assessing angiogenesis have primarily relied on measuring perfusion-related characteristics, such as total blood flow or microvascular volume, or detecting abnormal vascular permeability. Techniques are n...

journal_title：Expert review of molecular diagnostics

authors： Heppner P,Lindner JR

更新日期：2005-05-01 00:00:00

abstract：:Introduction: Human prion diseases are a heterogeneous group of incurable and debilitating conditions characterized by a progressive degeneration of the central nervous system. The conformational changes of the cellular prion protein and its formation into an abnormal isoform, spongiform degeneration, neuronal loss, a...

journal_title：Expert review of molecular diagnostics

authors： Thüne K,Schmitz M,Villar-Piqué A,Altmeppen HC,Schlomm M,Zafar S,Glatzel M,Llorens F,Zerr I

更新日期：2019-11-01 00:00:00

abstract：:A concise survey of the emerging PD-loop technology is presented, which outlines several exemplary methods with robust DNA diagnostic potential: duplex DNA capture, topological DNA labeling, nondenaturing DNA sequencing and hybridization of molecular beacons to double-stranded DNA. Advantages of these new PNA-based as...

journal_title：Expert review of molecular diagnostics

authors： Demidov VV

更新日期：2001-09-01 00:00:00

abstract：INTRODUCTION:Fine needle aspiration (FNA) with cytologic examination remains the standard of care for investigation of thyroid nodules. However, as many as 30% of FNA samples are cytologically indeterminate for malignancy, which confounds clinical management. To reduce the burden of repeat diagnostic testing and unnece...

journal_title：Expert review of molecular diagnostics

authors： Moore MD,Panjwani S,Gray KD,Finnerty BM,Zarnegar R,Fahey TJ 3rd

更新日期：2017-06-01 00:00:00

abstract：:The Presage(®) ST2 Assay (Critical Diagnostics, CA, USA) is an in vitro diagnostic device that quantitatively measures soluble suppression of tumorigenicity 2 (sST2) in serum and plasma by ELISA. This assay is US FDA approved and is indicated to be used in conjunction with clinical evaluation as an aid in assessing th...

journal_title：Expert review of molecular diagnostics

authors： Mueller T,Dieplinger B

更新日期：2013-01-01 00:00:00

abstract：:EU regulations stipulate the labeling of food products containing genetically modified organisms (GMOs) unless the GMO content is due to adventitious and unintended 'contamination' and not exceeding the 1% level at ingredient basis. In addition, member states have to ensure full traceability at all stages of the placi...

journal_title：Expert review of molecular diagnostics

authors： Aarts HJ,van Rie JP,Kok EJ

更新日期：2002-01-01 00:00:00

abstract：:Since the discovery of the link between miRNA and cancer, miRNAs have been investigated in virtually all tumors. Their ability to add a novel level of gene regulation and to target genes apparently not linked to each other has greatly intrigued researchers and physicians alike. In this review, the role of miRNAs in mu...

journal_title：Expert review of molecular diagnostics

authors： Rocci A,Hofmeister CC,Pichiorri F

更新日期：2014-11-01 00:00:00

abstract：:Prostate cancer (PCa) is one of the leading causes of cancer death among males, especially in more developed countries. Diagnosis is often achieved at an early stage of the disease with prostate biopsy, following a screening test showing elevated serum levels of prostate-specific antigen or a positive digital rectal e...

journal_title：Expert review of molecular diagnostics

authors： Kontos CK,Adamopoulos PG,Scorilas A

更新日期：2015-01-01 00:00:00

abstract：:The possibility to detect viral DNA or RNA in a quantitative manner has already contributed significantly to the management and diagnosis of viral infections, as well as to the understanding of virus-host interactions. New developments in amplification techniques based on real-time detection, as well as automation of ...

journal_title：Expert review of molecular diagnostics

authors： Schutten M,Niesters HG

更新日期：2001-07-01 00:00:00

abstract：:The emerging field of metabolomics, in which a large number of small-molecule metabolites from body fluids or tissues are detected quantitatively in a single step, promises immense potential for early diagnosis, therapy monitoring and for understanding the pathogenesis of many diseases. Metabolomics methods are mostly...

journal_title：Expert review of molecular diagnostics

authors： Gowda GA,Zhang S,Gu H,Asiago V,Shanaiah N,Raftery D

更新日期：2008-09-01 00:00:00

abstract：:Lipoprotein-associated phospholipase (Lp-PL)A2 is a recently described and potentially useful plasma biomarker associated with cardiovascular disease. The enzyme, originally named platelet-activating factor acetylhydrolase (PAF-AH), has two prominent biological activities. First, it inactivates the prominent proinflam...

journal_title：Expert review of molecular diagnostics

authors： Carlquist JF,Muhlestein JB,Anderson JL

更新日期：2007-09-01 00:00:00

abstract：INTRODUCTION:Rapidity, simplicity, and portability are highly desirable characteristics of tests and devices designed for performing diagnostics at the point of care (POC), either near patients managed in healthcare facilities or to offer bioanalytical alternatives in external settings. By reducing the turnaround time ...

journal_title：Expert review of molecular diagnostics

authors： Bissonnette L,Bergeron MG

更新日期：2017-05-01 00:00:00

abstract：:Tuberculosis (TB) is the most deadly infectious disease in the world. TB control relies on passive case findings and targeted treatment of latently infected individuals at high risk of disease progression. Tuberculin skin testing (TST) is conventionally used for detection of TB infection. Recently, blood assays measur...

journal_title：Expert review of molecular diagnostics

authors： Bocchino M,Bellofiore B,Matarese A,Galati D,Sanduzzi A

更新日期：2009-03-01 00:00:00

abstract：:Leptospirosis is a bacterial zoonotic disease that remains a public health problem worldwide. Unfortunately, the current gold standard test used for the serological diagnosis of leptospirosis has some drawbacks and there is no vaccine available for successful prevention of leptospirosis in humans. Therefore, recent re...

journal_title：Expert review of molecular diagnostics

authors： Thongboonkerd V

更新日期：2008-01-01 00:00:00

abstract：:Genetic mapping by linkage analysis has been an invaluable tool in the positional strategy to identify the molecular basis of many rare Mendelian disorders. With the attention of the scientific and medical community shifting towards the analysis of more common, complex traits, it has become necessary to develop new ap...

journal_title：Expert review of molecular diagnostics

authors： Forabosco P,Falchi M,Devoto M

更新日期：2005-09-01 00:00:00

abstract：BACKGROUND:The prognostic significance of serum gamma-glutamyl transferase (GGT) level at diagnosis in patients with metastatic non-small lung cancer (NSCLC) is not clear. We aimed to assess the relationship between serum GGT level and overall survival (OS) and progression-free survival (PFS) in this patient population...

journal_title：Expert review of molecular diagnostics

authors： Bozkaya Y,Yazıcı O

更新日期：2019-03-01 00:00:00

abstract：INTRODUCTION:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful new tool to identify human fungal pathogens and has radically altered the diagnostic mycology workflow at many medical centers around the world. Areas covered: While most experience is with...

journal_title：Expert review of molecular diagnostics

authors： Walsh TJ,McCarthy MW

更新日期：2019-03-01 00:00:00

abstract：:Colorectal cancer (CRC) is a slow-developing cancer (10-15 years) with one of the highest frequencies in the world's population. Many countries have implemented various CRC screening programs, but have not achieved the desired compliance. Colonoscopy - considered the gold standard for CRC screening - has its limitatio...

journal_title：Expert review of molecular diagnostics

authors： Molnár B,Tóth K,Barták BK,Tulassay Z

更新日期：2015-02-01 00:00:00

abstract：:Several biomarkers associated with spontaneous preterm birth (PTB) have been discovered over the last decade. Many of these markers, such as cytokines, are associated with infection and inflammation. As such, these biomarkers represent biologically plausible candidates for assessing those at risk of PTB. However, in t...

journal_title：Expert review of molecular diagnostics

authors： Williams SM,Velez DR,Menon R

更新日期：2010-01-01 00:00:00

abstract：:Evaluation of: Wang WS, Liu LX, Li GP et al. Combined serum CA19-9 and miR-27a-3p in peripheral blood mononuclear cells to diagnose pancreatic cancer. Cancer Prev. Res. (Phila.) 6(4), 331-338 (2013). Patients with pancreatic ductal adenocarcinoma (PDAC) have a bleak outlook, primarily because tumors are detected late ...

journal_title：Expert review of molecular diagnostics

authors： Frampton AE,Fletcher CE,Gall TM,Castellano L,Bevan CL,Stebbing J,Krell J

更新日期：2013-06-01 00:00:00

abstract：:Parkinson's disease (PD) is a common, heterogeneous syndrome diagnosed clinically by the presence of classical neurological symptoms and the absence of 'red flags' that suggest alternative secondary parkinsonian disorders. Neuropathologically, nigrostriatal loss and the presence of proteinaceous inclusions (Lewy bodie...

journal_title：Expert review of molecular diagnostics

authors： Mellick GD,Silburn PA,Sutherland GT,Siebert GA

更新日期：2010-11-01 00:00:00

abstract：:Since aptamers were reported in 1990, research into the applications of aptamers, particularly diagnostic applications, has been growing. Aptamers can act as recognition elements instead of antibodies. In this regard, aptamers have unique characteristics because they are composed of nucleic acids. Intra- and intermole...

journal_title：Expert review of molecular diagnostics

authors： Yoshida W,Abe K,Ikebukuro K

更新日期：2014-03-01 00:00:00

abstract：:The management of cancer and other genetically based diseases is far from optimal in even our most advanced medical centers. There is still uncertainty regarding how diseases will progress in certain patients, toxicity that must be tolerated with imprecise treatment regimens and significant potential for treatment fai...

journal_title：Expert review of molecular diagnostics

authors： Best CJ,Emmert-Buck MR

更新日期：2001-05-01 00:00:00

abstract：:In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases. This molecular tool is well-suited for the rapid detection of bacteria directly in clinical specimens, allowing early, s...

journal_title：Expert review of molecular diagnostics

authors： Maurin M

更新日期：2012-09-01 00:00:00

abstract：:Klinefelter syndrome is the most common chromosome abnormality in humans. The estimated prevalence is one in 500 to one in 1000 males but due to the widely variable and often aspecific features, only one in four cases are recognized. The most specific clinical features which can be observed at adult age are small test...

journal_title：Expert review of molecular diagnostics

authors： Giltay JC,Maiburg MC

更新日期：2010-09-01 00:00:00

abstract：:Background: We analyzed the correlation between heart-type fatty acid-binding protein (HFABP) and the development of acute kidney injury (AKI) in patients with sepsis and estimated the predictive capacity of HFABP for sepsis-associated acute kidney injury (SAKI). Methods: In this retrospective observational study, we ...

journal_title：Expert review of molecular diagnostics

authors： Jiang D,Shen M,Yuan X,Wang M,Li S,Jiang W,Zhou Z,Xi P,Wang T,Shen Y

更新日期：2019-08-01 00:00:00

abstract：:Reverse-transcription PCR (RT-PCR) coupled with electrospray ionization mass spectrometry (ESI-MS) is a high-throughput nucleic acid-based technology that relies on the accurate measurement of the molecular weight of PCR amplicons that can be used to deduce the base counts (number of As, Gs, Cs and Ts) of DNA. These a...

journal_title：Expert review of molecular diagnostics

authors： Deyde VM,Sampath R,Gubareva LV

更新日期：2011-01-01 00:00:00

abstract：:Fluorescence in situ hybridization, spectral karyotyping, multiplex fluorescence in situ hybridization, comparative genomic hybridization, and more recently array comparative genomic hybridization, represent advancements in the field of molecular cytogenetics. The application of these techniques for the analysis of sp...

journal_title：Expert review of molecular diagnostics

authors： Dorritie K,Montagna C,Difilippantonio MJ,Ried T

更新日期：2004-09-01 00:00:00