RT-PCR/electrospray ionization mass spectrometry approach in detection and characterization of influenza viruses.

Abstract:

:Reverse-transcription PCR (RT-PCR) coupled with electrospray ionization mass spectrometry (ESI-MS) is a high-throughput nucleic acid-based technology that relies on the accurate measurement of the molecular weight of PCR amplicons that can be used to deduce the base counts (number of As, Gs, Cs and Ts) of DNA. These amplicons represent highly variable regions with information-rich sequences, which are flanked by broad-range primers designed based on highly conserved loci. This technology was first introduced in 2005 for microbial identification and subtyping, and was later applied to influenza virus detection and identification. The influenza RT-PCR/ESI-MS assay allows analysis of approximately 300 samples per 24 h, and aids in the characterization of influenza viruses based on their 'core' gene signatures. Notably, this assay was used to identify one of the first cases of the 2009 H1N1 pandemic viruses. One of the main advantages of the RT-PCR/ESI-MS technology is its universality and adaptability for pathogen characterization. Efforts are being made to customize the currently used influenza surveillance assay for use in the diagnosis of the H1N1 pandemic virus. In this article, we provide a summary of known applications of the RT-PCR/ESI-MS assay in the field of influenza.

journal_name

Expert Rev Mol Diagn

authors

Deyde VM,Sampath R,Gubareva LV

doi

10.1586/erm.10.107

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

41-52

issue

1

eissn

1473-7159

issn

1744-8352

journal_volume

11

pub_type

杂志文章,评审
  • Next-generation sequencing in precision oncology: challenges and opportunities.

    abstract::High throughput gene sequencing is transforming the utilization of genomics in patient care by providing physicians with a powerful tool to aid the diagnosis and management of disease, particularly in precision oncology. As next-generation sequencing (NGS)-based diagnostic assays are developed, significant hurdles suc...

    journal_title:Expert review of molecular diagnostics

    pub_type: 社论

    doi:10.1586/14737159.2014.916213

    authors: Kruglyak KM,Lin E,Ong FS

    更新日期:2014-07-01 00:00:00

  • Current and future molecular diagnostics in non-small-cell lung cancer.

    abstract::The molecular investigation of lung cancer has opened up an advanced area for the diagnosis and therapeutic management of lung cancer patients. Gene alterations in cancer initiation and progression provide not only information on molecular changes in lung cancer but also opportunities in advanced therapeutic regime by...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.2015.1063420

    authors: Li CM,Chu WY,Wong DL,Tsang HF,Tsui NB,Chan CM,Xue VW,Siu PM,Yung BY,Chan LW,Wong SC

    更新日期:2015-01-01 00:00:00

  • Implications of genomic instability in the diagnosis and treatment of breast cancer.

    abstract::Tumorigenesis is a multistep process resulting from DNA mutations observed at the DNA sequence and chromosome level as well as epigenetic changes, which affect expression of oncogenes and tumor suppressor genes. Breast cancer is a very heterogeneous disease that manifests in various histological and clinical types. De...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.11.21

    authors: Wiechec E

    更新日期:2011-05-01 00:00:00

  • Genomic and proteomic approaches for Chagas' disease: critical analysis of diagnostic methods.

    abstract::Trypanosoma cruzi is the etiologic agent of Chagas' disease, a chronic inflammatory condition that results in heart and digestive complications. The first draft of the parasite genome is now complete and it is expected that, along with the published genomic and proteomic analyses discussed herein, it will lead to the ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.5.4.521

    authors: Huete-Pérez JA,Flores-Obando RE,Ghedin E,Caffrey CR

    更新日期:2005-07-01 00:00:00

  • Plasma methylated septin 9: a colorectal cancer screening marker.

    abstract::Colorectal cancer (CRC) is a slow-developing cancer (10-15 years) with one of the highest frequencies in the world's population. Many countries have implemented various CRC screening programs, but have not achieved the desired compliance. Colonoscopy - considered the gold standard for CRC screening - has its limitatio...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章

    doi:10.1586/14737159.2015.975212

    authors: Molnár B,Tóth K,Barták BK,Tulassay Z

    更新日期:2015-02-01 00:00:00

  • Application of mass spectrometry to molecular diagnostics of viral infections.

    abstract::Mass spectrometry (MS) has found numerous applications in life sciences. It has high accuracy, sensitivity and wide dynamic range in addition to medium- to high-throughput capabilities. These features make MS a superior platform for analysis of various biomolecules including proteins, lipids, nucleic acids and carbohy...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.13.24

    authors: Ganova-Raeva LM,Khudyakov YE

    更新日期:2013-05-01 00:00:00

  • Metabolomics-based methods for early disease diagnostics.

    abstract::The emerging field of metabolomics, in which a large number of small-molecule metabolites from body fluids or tissues are detected quantitatively in a single step, promises immense potential for early diagnosis, therapy monitoring and for understanding the pathogenesis of many diseases. Metabolomics methods are mostly...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.8.5.617

    authors: Gowda GA,Zhang S,Gu H,Asiago V,Shanaiah N,Raftery D

    更新日期:2008-09-01 00:00:00

  • Label-free mass spectrometry-based proteomics for biomarker discovery and validation.

    abstract::For most diseases, better biomarkers are urgently needed to enable (early) detection, diagnosis, prognosis, stratification for therapy and response monitoring. Proteomics delineates gene products that carry out the majority of cellular functions, and thereby may not only yield insight into altered signaling pathways i...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.12.31

    authors: Pham TV,Piersma SR,Oudgenoeg G,Jimenez CR

    更新日期:2012-05-01 00:00:00

  • Diagnostic potential of peritoneal fluid biomarkers of endometriosis.

    abstract::Endometriosis is defined as the presence of endometrial glands and stroma outside the uterus, in different parts of the peritoneal cavity. It affects up to 10% of reproductive-age women and up to 50% of women with infertility. Surgical diagnosis of endometriosis is still the gold standard, with no diagnostic biomarker...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.2015.1015994

    authors: Rižner TL

    更新日期:2015-04-01 00:00:00

  • Exploiting the potential of molecular profiling in Parkinson's disease: current practice and future probabilities.

    abstract::Parkinson's disease (PD) is a common, heterogeneous syndrome diagnosed clinically by the presence of classical neurological symptoms and the absence of 'red flags' that suggest alternative secondary parkinsonian disorders. Neuropathologically, nigrostriatal loss and the presence of proteinaceous inclusions (Lewy bodie...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.10.86

    authors: Mellick GD,Silburn PA,Sutherland GT,Siebert GA

    更新日期:2010-11-01 00:00:00

  • Laser capture microdissection technology.

    abstract::Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions through molecular analysis, one must be able to analyze specific cell populations within the context of t...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.7.5.647

    authors: Espina V,Heiby M,Pierobon M,Liotta LA

    更新日期:2007-09-01 00:00:00

  • Molecular profiling of tissue samples using laser capture microdissection.

    abstract::The management of cancer and other genetically based diseases is far from optimal in even our most advanced medical centers. There is still uncertainty regarding how diseases will progress in certain patients, toxicity that must be tolerated with imprecise treatment regimens and significant potential for treatment fai...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.1.1.53

    authors: Best CJ,Emmert-Buck MR

    更新日期:2001-05-01 00:00:00

  • Epigenetic alteration of microRNAs in feces of colorectal cancer and its clinical significance.

    abstract::MicroRNAs regulate target gene expression through translation repression or mRNA decay, and they are emerging as important modulators in cellular pathways. Previous studies have shown the occurrence of epigenetically modified miRNAs in colorectal cancer (CRC), identifying these miRNA methylation signatures may provide...

    journal_title:Expert review of molecular diagnostics

    pub_type: 评论,杂志文章

    doi:10.1586/erm.11.57

    authors: Cho WC

    更新日期:2011-09-01 00:00:00

  • Update on bipolar disorder biomarker candidates.

    abstract:INTRODUCTION:Bipolar disorder is a chronic and disabling mood disorder with a complex pathophysiological basis. A significant percentage of patients do not receive correct diagnosis which directly influences therapeutic response, rendering recovery troublesome. There is a long-standing need for proper non-clinically ba...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1080/14737159.2016.1248413

    authors: Teixeira AL,Salem H,Frey BN,Barbosa IG,Machado-Vieira R

    更新日期:2016-11-01 00:00:00

  • Advances in clinical next-generation sequencing: target enrichment and sequencing technologies.

    abstract::The huge parallel sequencing capabilities of next generation sequencing technologies have made them the tools of choice to characterize genomic aberrations for research and diagnostic purposes. For clinical applications, screening the whole genome or exome is challenging owing to the large genomic area to be sequenced...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.2016.1133298

    authors: Ballester LY,Luthra R,Kanagal-Shamanna R,Singh RR

    更新日期:2016-01-01 00:00:00

  • Klinefelter syndrome: clinical and molecular aspects.

    abstract::Klinefelter syndrome is the most common chromosome abnormality in humans. The estimated prevalence is one in 500 to one in 1000 males but due to the widely variable and often aspecific features, only one in four cases are recognized. The most specific clinical features which can be observed at adult age are small test...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.10.63

    authors: Giltay JC,Maiburg MC

    更新日期:2010-09-01 00:00:00

  • Information management driven by diagnostic patient data: right information for the right patient.

    abstract::Existing and future diagnostic technologies are providing a huge amount of data about the patient, which are to be sensibly managed by the treating physician. More than that, the sheer complexity of the interrelation between these data are calling for a radically new approach in the handling of medical data and inform...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.2.4.355

    authors: Fierz W

    更新日期:2002-07-01 00:00:00

  • Molecular diagnostics in the management of rhabdomyosarcoma.

    abstract:INTRODUCTION:A classification of rhabdomyosarcoma (RMS) with prognostic relevance has primarily relied on clinical features and histologic classification as either embryonal or alveolar RMS. The PAX3-FOXO1 and PAX7-FOXO1 gene fusions occur in 80% of cases with the alveolar subtype and are more predictive of outcome tha...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1080/14737159.2017.1275965

    authors: Arnold MA,Barr FG

    更新日期:2017-02-01 00:00:00

  • Theranostics meets traditional Chinese medicine: rational prediction of drug-herb interactions.

    abstract::Herbal medicines including traditional Chinese medicine are becoming increasingly more popular worldwide. However, there is considerable potential for interaction between herbal components and drugs, as all herbal medicines contain a combination of potentially biologically active compounds possessing various inherent ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.12.126

    authors: Hu M,Fan L,Zhou HH,Tomlinson B

    更新日期:2012-11-01 00:00:00

  • Genomic characterization reveals potential biomarkers in nasopharyngeal carcinoma patients with relapse.

    abstract:BACKGROUND:Although the majority of nasopharyngeal carcinoma (NPC) patients demonstrate favorable outcomes after radiotherapy and/or chemotherapy, about 8-10% of patients will develop recurrent disease, and genomic alterations (GAs) associated with the recurrence are unclear. METHODS:This study investigated the GAs in...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章

    doi:10.1080/14737159.2020.1835473

    authors: Cho WCS,Tse KP,Ngan RKC,Cheuk W,Ma VWS,Yang YT,Yip TTC,Tan KT,Chen SJ

    更新日期:2020-11-01 00:00:00

  • Serum heart-type fatty acid-binding protein as a predictor for the development of sepsis-associated acute kidney injury.

    abstract::Background: We analyzed the correlation between heart-type fatty acid-binding protein (HFABP) and the development of acute kidney injury (AKI) in patients with sepsis and estimated the predictive capacity of HFABP for sepsis-associated acute kidney injury (SAKI). Methods: In this retrospective observational study, we ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章

    doi:10.1080/14737159.2019.1642750

    authors: Jiang D,Shen M,Yuan X,Wang M,Li S,Jiang W,Zhou Z,Xi P,Wang T,Shen Y

    更新日期:2019-08-01 00:00:00

  • Portable devices and mobile instruments for infectious diseases point-of-care testing.

    abstract:INTRODUCTION:Rapidity, simplicity, and portability are highly desirable characteristics of tests and devices designed for performing diagnostics at the point of care (POC), either near patients managed in healthcare facilities or to offer bioanalytical alternatives in external settings. By reducing the turnaround time ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1080/14737159.2017.1310619

    authors: Bissonnette L,Bergeron MG

    更新日期:2017-05-01 00:00:00

  • Geographic ancestry and markers of preterm birth.

    abstract::Several biomarkers associated with spontaneous preterm birth (PTB) have been discovered over the last decade. Many of these markers, such as cytokines, are associated with infection and inflammation. As such, these biomarkers represent biologically plausible candidates for assessing those at risk of PTB. However, in t...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.09.70

    authors: Williams SM,Velez DR,Menon R

    更新日期:2010-01-01 00:00:00

  • Management of metastatic renal cell carcinoma: current trends.

    abstract::Renal cell carcinoma is one of the common malignancies of the genitourinary tract. In approximately one third of patients, distant metastases are present at the time of initial diagnosis and in another third, the tumor will recur even after nephrectomy with a curative intent. Renal cell carcinoma is resistant to all c...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.9.1.75

    authors: Mohammed A,Shergill I,Little B

    更新日期:2009-01-01 00:00:00

  • Basic principles of real-time quantitative PCR.

    abstract::Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications. This exciting technology has ...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.5.2.209

    authors: Arya M,Shergill IS,Williamson M,Gommersall L,Arya N,Patel HR

    更新日期:2005-03-01 00:00:00

  • Mutation testing for directing upfront targeted therapy and post-progression combination therapy strategies in lung adenocarcinoma.

    abstract:INTRODUCTION:Advances in the biology of non-small-cell lung cancer, especially adenocarcinoma, reveal multiple molecular subtypes driving oncogenesis. Accordingly, individualized targeted therapeutics are based on mutational diagnostics. AREAS COVERED:Advances in strategies and techniques for individualized treatment,...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1080/14737159.2016.1181545

    authors: Salgia R

    更新日期:2016-07-01 00:00:00

  • Clinical implications of and lessons learnt from external assessment of Mers-CoV diagnostics.

    abstract::With the emergence of new viral infections, it is necessary to set up new target-specific assays, based on existing molecular techniques such as real-time PCR, as quickly as possible. Without these diagnostic tools, the geographical spread of new infections, follow-up of the disease outbreak and analysis of the pathog...

    journal_title:Expert review of molecular diagnostics

    pub_type: 社论

    doi:10.1586/14737159.2016.1116943

    authors: Pas SD,Koopmans MP,Niedrig M

    更新日期:2016-01-01 00:00:00

  • Accuracy of early diagnosis and its impact on the management and course of Alzheimer's disease.

    abstract::Alzheimer's disease is the most common form of dementia in the elderly and its prevalence is rapidly rising. Although there is no cure for Alzheimer's disease, treatment can be administered to slow progression or delay the onset of symptoms. A major challenge is the early identification of patients who will develop Al...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/14737159.4.1.63

    authors: Chang CY,Silverman DH

    更新日期:2004-01-01 00:00:00

  • High-resolution DNA melting analysis in clinical research and diagnostics.

    abstract::Among nucleic acid analytical methods, high-resolution melting analysis is gaining more and more attention. High-resolution melting provides simple, homogeneous solutions for variant scanning and genotyping, addressing the needs of today's overburdened laboratories with rapid turnaround times and minimal cost. The fle...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1586/erm.09.84

    authors: Montgomery JL,Sanford LN,Wittwer CT

    更新日期:2010-03-01 00:00:00

  • The clinical implementation of copy number detection in the age of next-generation sequencing.

    abstract:INTRODUCTION:The role of copy number variants (CNVs) in disease is now well established. In parallel NGS technologies, such as long-read technologies, there is continual development and data analysis methods continue to be refined. Clinical exome sequencing data is now a reality for many diagnostic laboratories in both...

    journal_title:Expert review of molecular diagnostics

    pub_type: 杂志文章,评审

    doi:10.1080/14737159.2018.1523723

    authors: Hehir-Kwa JY,Tops BBJ,Kemmeren P

    更新日期:2018-10-01 00:00:00