Abstract:
:Understanding how virus capsids assemble around their nucleic acid (NA) genomes could promote efforts to block viral propagation or to reengineer capsids for gene therapy applications. We develop a coarse-grained model of capsid proteins and NAs with which we investigate assembly dynamics and thermodynamics. In contrast to recent theoretical models, we find that capsids spontaneously 'overcharge'; that is, the negative charge of the NA exceeds the positive charge on capsid. When applied to specific viruses, the optimal NA lengths closely correspond to the natural genome lengths. Calculations based on linear polyelectrolytes rather than base-paired NAs underpredict the optimal length, demonstrating the importance of NA structure to capsid assembly. These results suggest that electrostatics, excluded volume, and NA tertiary structure are sufficient to predict assembly thermodynamics and that the ability of viruses to selectively encapsidate their genomic NAs can be explained, at least in part, on a thermodynamic basis. DOI:http://dx.doi.org/10.7554/eLife.00632.001.
journal_name
Elifejournal_title
eLifeauthors
Perlmutter JD,Qiao C,Hagan MFdoi
10.7554/eLife.00632subject
Has Abstractpub_date
2013-06-14 00:00:00pages
e00632issn
2050-084Xpii
00632journal_volume
2pub_type
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