Abstract：
:A total of 18 protease producing bacterial strains were isolated from detergent effluent in South Korea using skim milk agar medium. A strain (BK-P21A) was selected and identified as Bacillus koreensis based on morphological, biochemical and molecular characterizations (16S rRNA gene sequence analysis). Optimized culture conditions for the production of protease were pH 8.5, 30 °C, sucrose (2%) and yeast extract (0.2%) during 36 h of incubation. Furthermore, the protease was partially purified by ammonium sulphate precipitation (80%) and again by Superdex 200 10/300 GL and Superdex 75 10/300 GL column chromatography, which resulted in 5.0 fold purification and a yield of 23%. The molecular mass of the protease was estimated to be 48 kDa by SDS-PAGE. The purified enzyme was further characterized and found to be most active at pH 9.0 and 60 °C. The activity of the purified protease was enhanced by CaCl₂ and CoCl₂, but inhibited by PMSF, which indicated it was a serine type protease. Moreover, the protease was moderately stable in surfactants and 81% stable in H₂O₂. Finally, the enzyme was more active and stable (94-126.5%) in various hydrophilic organic solvents. Considering the stability of protease towards the alkaline pH, high temperature and organic solvents (50%), the enzyme from B. koreensis can be used as an alternative biocatalyst for several industrial applications mainly for peptide synthesis in nonaqueous solvents.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Anbu Pdoi
10.1016/j.ijbiomac.2013.02.014subject
Has Abstractpub_date
2013-05-01 00:00:00pages
162-8eissn
0141-8130issn
1879-0003pii
S0141-8130(13)00066-4journal_volume
56pub_type
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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更新日期：2019-09-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(00)00132-x
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2001-04-12 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2015-05-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.03.034
更新日期：2014-06-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.05.061
更新日期：2016-10-01 00:00:00
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journal_title：International journal of biological macromolecules
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更新日期：2018-03-01 00:00:00
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2002-03-08 00:00:00
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journal_title：International journal of biological macromolecules
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更新日期：2021-01-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-12-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：1992-12-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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更新日期：2016-12-01 00:00:00
abstract：:As one of the most promising biopolymers for a variety of potential applications, chitosan has attracted much attention because of its unique biological, chemical, and physical properties. The functionalization of chitosan has been adopted to synthesize novel chitosan derivatives with improved water-solubility and exc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.019
更新日期：2020-12-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.216
更新日期：2019-10-15 00:00:00
abstract：:A novel esterase, EstH was cloned, purified and characterized from the marine bacterium Zunongwangia sp. The purified EstH showed optimum activity at 30°C and pH 8.5 with ∼50% of original activity at 0°C. EstH was stable in high salt conditions (0-4.5M NaCl). To improve the characteristics and explore the possibilitie...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.03.016
更新日期：2016-06-01 00:00:00
