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A molecular-beacon-based screen for small molecule inhibitors of miRNA maturation.

Abstract：

:miRNAs are small non-coding RNAs that regulate about 60% of mammalian genes by modulating their transcript levels. Network scale studies of miRNA-mediated regulatory circuits demonstrate the central importance of this class of small RNA in the maintenance of biological robustness. More recently, several reports have described the deregulation of numerous miRNA to be causally associated with many diseases, including cancer. These studies have highlighted the potential for development of therapeutic modalities against miRNA. Previous screening protocols, for small molecules targeting miRNA function, are either costly or technically too complex to be applied in a high-throughput manner in standard chemical laboratories. We describe a simple in vitro screening method using a DNA-based molecular beacon that overcomes the limitations associated with earlier screens. We used this method to identify inhibitors of miR-27a function from a library of 14 aminoglycosides as a pilot study. Inhibitory molecules identified were further scrutinized to identify the validity of screen. With this proof of concept we illustrate the utility of a scalable molecular-beacon-based screening strategy for miRNA inhibitors.

journal_name

ACS Chem Biol

journal_title

ACS chemical biology

authors

Bose D,Jayaraj GG,Kumar S,Maiti S

doi

10.1021/cb300650y

subject

Has Abstract

pub_date

2013-05-17 00:00:00

pages

930-8

issue

5

eissn

1554-8929

issn

1554-8937

journal_volume

8

pub_type

杂志文章

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