Abstract:
:Because of their limited regenerative potential, cochlear hair cell loss is one of the major causes of permanent hearing loss in mammals. However, recent studies have shown that postnatal cochlear epithelia retain the progenitor cells that form otospheres. Otospheres are capable of self-renewing and differentiating into inner ear cell lineages, thereby suggesting a promising source for hair cell regeneration. We investigated retention of the progenitor cell phenotype in otospheres after ex vivo expansion, which is crucial for transplantation approaches. Reverse transcriptase-polymerase chain reaction and immunocytochemical analyses showed that otospheres derived from neonatal mice retained expression of stem and cochlear cell markers. After in vitro differentiation, otosphere-consisting cells differentiated into hair cell phenotypes after ex vivo expansion. However, the capacity of otospheres for self-renewal weakened with subsequent generations of ex vivo expansion. Our results indicate that ex vivo expanded-otospheres are useful experimental tools for studying hair cell regeneration in transplantation approaches and that the mechanisms for retention of the progenitor cell phenotype in otospheres should be investigated.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Lou XX,Nakagawa T,Ohnishi H,Nishimura K,Ito Jdoi
10.1016/j.neulet.2012.12.001subject
Has Abstractpub_date
2013-02-08 00:00:00pages
18-23eissn
0304-3940issn
1872-7972pii
S0304-3940(12)01518-2journal_volume
534pub_type
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