当前位置： SCI文献检索 > OSTEOARTHRITIS AND CARTILAGE期刊下所有文献 > Chondrocytes extract from patients with osteoarthritis induces chondrogenesis in infrapatellar fat pad-derived stem cells.

Chondrocytes extract from patients with osteoarthritis induces chondrogenesis in infrapatellar fat pad-derived stem cells.

Abstract：

OBJECTIVE:Infrapatellar fat pad of patients with osteoarthritis (OA) contains multipotent and highly clonogenic adipose-derived stem cells that can be isolated by low invasive methods. Moreover, nuclear and cytoplasmic cellular extracts have been showed to be effective in induction of cell differentiation and reprogramming. The aim of this study was to induce chondrogenic differentiation of autologous mesenchymal stem cells (MSCs) obtained from infrapatellar fat pad (IFPSCs) of patients with OA using cellular extracts-based transdifferentiation method. DESIGN:IFPSCs and chondrocytes were isolated and characterized by flow cytometry. IFPSCs were permeabilized with Streptolysin O and then exposed to a cell extract obtained from chondrocytes. Then, IFPSCs were cultured for 2 weeks and chondrogenesis was evaluated by morphologic and ultrastructural observations, immunologic detection, gene expression analysis and growth on 3-D poly (dl-lactic-co-glycolic acid) (PLGA) scaffolds. RESULTS:After isolation, both chondrocytes and IFPSCs displayed similar expression of MSCs surface makers. Collagen II was highly expressed in chondrocytes and showed a basal expression in IFPSCs. Cells exposed to chondrocyte extracts acquired a characteristic morphological and ultrastructural chondrocyte phenotype that was confirmed by the increased proteoglycan formation and enhanced collagen II immunostaining. Moreover, chondrocyte extracts induced an increase in mRNA expression of chondrogenic genes such as Sox9, L-Sox5, Sox6 and Col2a1. Interestingly, chondrocytes, IFPSCs and transdifferentiated IFPSCs were able to grow, expand and produce extracellular matrix (ECM) on 3D PLGA scaffolds. CONCLUSIONS:We demonstrate for the first time that extracts obtained from chondrocytes of osteoarthritic knees promote chondrogenic differentiation of autologous IFPSCs. Moreover, combination of transdifferentiated IFPSCs with biodegradable PLGA 3D scaffolds can serve as an efficient system for the maintenance and maturation of cartilage tissue. These findings suggest its usefulness to repair articular surface in OA.

journal_name

Osteoarthritis Cartilage

journal_title

Osteoarthritis and cartilage

authors

López-Ruiz E,Perán M,Cobo-Molinos J,Jiménez G,Picón M,Bustamante M,Arrebola F,Hernández-Lamas MC,Delgado-Martínez AD,Montañez E,Marchal JA

doi

10.1016/j.joca.2012.10.007

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

246-58

issue

1

eissn

1063-4584

issn

1522-9653

pii

S1063-4584(12)01001-1

journal_volume

21

pub_type

杂志文章

相关文献

OSTEOARTHRITIS AND CARTILAGE文献大全
  • Exercise-driven metabolic pathways in healthy cartilage.

    abstract：OBJECTIVE:Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. METHODS:Transcriptome-wide gene expression...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.02.004

    authors： Blazek AD,Nam J,Gupta R,Pradhan M,Perera P,Weisleder NL,Hewett TE,Chaudhari AM,Lee BS,Leblebicioglu B,Butterfield TA,Agarwal S

    更新日期：2016-07-01 00:00:00

  • The chondrogenic potential of carbon fiber and carbon fiber periosteum implants: an ultrastructural study in the rabbit.

    abstract：:The articular tissue generated on carbon fiber rods in rabbit knee joints after 75 days of intermittent active motion was compared with tissue generated on carbon fiber rods whose articular surfaces were covered with a free reversed periosteal graft. Both methods were effective in generating articular tissue; however,...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(05)80077-9

    authors： Curtin W,Reville W,Heapes M,Lyons J,Muckle D

    更新日期：1994-12-01 00:00:00

  • Thinning of articular cartilage after joint unloading or immobilization. An experimental investigation of the pathogenesis in mice.

    abstract：OBJECTIVE:Moderate mechanical stress generated by normal joint loading and movement is essential for the maintenance of healthy articular cartilage. However, the effects of reduced loading caused by the absence of weight bearing or joint motion on articular cartilage and subchondral bone is still poorly understood. We ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.11.013

    authors： Nomura M,Sakitani N,Iwasawa H,Kohara Y,Takano S,Wakimoto Y,Kuroki H,Moriyama H

    更新日期：2017-05-01 00:00:00

  • Human genome-wide expression analysis reorients the study of inflammatory mediators and biomechanics in osteoarthritis.

    abstract：:A major objective of this article is to examine the research implications of recently available genome-wide expression profiles of cartilage from human osteoarthritis (OA) joints. We propose that, when viewed in the light of extensive earlier work, this novel data provides a unique opportunity to reorient the design o...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2015.03.027

    authors： Sandy JD,Chan DD,Trevino RL,Wimmer MA,Plaas A

    更新日期：2015-11-01 00:00:00

  • Gene therapy for meniscal injury: enhanced synthesis of proteoglycan and collagen by meniscal cells transduced with a TGFbeta(1)gene.

    abstract：:Objective To determine whether meniscal cells can express a TGFbeta(1)transgene delivered by a retroviral vector, and respond to the gene product by increasing matrix synthesis. Methods Monolayer cultures of human and canine meniscal cells were infected with retroviruses carrying either a human TGFbeta(1)cDNA or marke...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.1999.0300

    authors： Goto H,Shuler FD,Niyibizi C,Fu FH,Robbins PD,Evans CH

    更新日期：2000-07-01 00:00:00

  • Associations among preoperative MRI features and functional status following arthroscopic partial meniscectomy.

    abstract：BACKGROUND:Arthroscopic partial meniscectomy (APM) is the most frequently performed orthopedic procedure. Functional outcomes of APM are variable, particularly among patients with underlying knee osteoarthritis. While most patients undergoing APM have knee magnetic resonance imaging (MRI) performed preoperatively, the ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2005.11.014

    authors： Katz JN,Meredith DS,Lang P,Creel AH,Yoshioka H,Neumann G,Fossel AH,de Pablo P,Losina E

    更新日期：2006-05-01 00:00:00

  • Correlation between the MR T2 value at 4.7 T and relative water content in articular cartilage in experimental osteoarthritis induced by ACL transection.

    abstract：OBJECTIVE:Both animal and human studies using magnetic resonance imaging (MRI) show that cartilage degeneration increases the T2 value. However, it is unclear whether the T2 value correlates linearly with water content in cartilage with osteoarthritis. The purpose of this study was to investigate the relationship betwe...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2008.09.009

    authors： Chou MC,Tsai PH,Huang GS,Lee HS,Lee CH,Lin MH,Lin CY,Chung HW

    更新日期：2009-04-01 00:00:00

  • Tumor risk by tissue engineering: cartilaginous differentiation of mesenchymal stem cells reduces tumor growth.

    abstract：OBJECTIVE:Implantation of autologous chondrocytes (AC) is a promising option for the treatment of cartilage defects, but problems with cell harvesting, dedifferentiation, or the donor age limit the clinical outcome. Mesenchymal stem cells (MSC) gain much interest because of their simple isolation and multipotential dif...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2009.10.015

    authors： Akay I,Oxmann D,Helfenstein A,Mentlein R,Schünke M,Hassenpflug J,Kurz B

    更新日期：2010-03-01 00:00:00

  • Dimensionality and clinical importance of pain and disability in hand osteoarthritis: Development of the Australian/Canadian (AUSCAN) Osteoarthritis Hand Index.

    abstract：OBJECTIVE:To develop a reliable, valid, and responsive self-administered questionnaire to probe pain, stiffness and physical disability in patients with osteoarthritis (OA) of the hand. DESIGN:In order to assess the dimensionality of the symptomatology of hand OA, a self-administered questionnaire was developed to pro...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1053/joca.2002.0837

    authors： Bellamy N,Campbell J,Haraoui B,Buchbinder R,Hobby K,Roth JH,MacDermid JC

    更新日期：2002-11-01 00:00:00

  • Identification and pharmacological characterization of a novel inhibitor of autotaxin in rodent models of joint pain.

    abstract：OBJECTIVE:Autotaxin is a secreted lysophospholipase that mediates the conversion of lysophosphatidyl choline (LPC) to lysophosphatidic acid (LPA), a bioactive lipid mediator. Autotaxin levels in plasma and synovial fluid correlate with disease severity in patients with knee osteoarthritis (OA). The goal of this study w...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.09.006

    authors： Thirunavukkarasu K,Swearingen CA,Oskins JL,Lin C,Bui HH,Jones SB,Pfeifer LA,Norman BH,Mitchell PG,Chambers MG

    更新日期：2017-06-01 00:00:00

  • Trabecular bone structure and spatial differences in articular cartilage MR relaxation times in individuals with posterior horn medial meniscal tears.

    abstract：OBJECTIVE:To analyze knee trabecular bone structure and spatial cartilage T(1ρ) and T(2) relaxation times using 3-T magnetic resonance imaging (MRI) in subjects with and without tears of posterior horn of the medial meniscus (PHMM). DESIGN:3-T MRI from 59 subjects (>18 years), were used to evaluate PHMM tears based on...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2012.09.014

    authors： Kumar D,Schooler J,Zuo J,McCulloch CE,Nardo L,Link TM,Li X,Majumdar S

    更新日期：2013-01-01 00:00:00

  • The control of cell division in articular chondrocytes.

    abstract：:Our objective was to investigate the factors that control chondrocyte division, namely, (1) steric hindrance caused by the surrounding matrix, (2) growth factors and (3) cell shape. Bovine articular cartilage explants and isolated chondrocytes were cultured in medium containing [3H]-TdR to measure DNA synthesis. The e...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(05)80029-9

    authors： Lee DA,Bentley G,Archer CW

    更新日期：1993-04-01 00:00:00

  • External knee adduction and flexion moments during gait and medial tibiofemoral disease progression in knee osteoarthritis.

    abstract：OBJECTIVE:Test the hypothesis that greater baseline peak external knee adduction moment (KAM), KAM impulse, and peak external knee flexion moment (KFM) during the stance phase of gait are associated with baseline-to-2-year medial tibiofemoral cartilage damage and bone marrow lesion progression, and cartilage thickness ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2015.02.005

    authors： Chang AH,Moisio KC,Chmiel JS,Eckstein F,Guermazi A,Prasad PV,Zhang Y,Almagor O,Belisle L,Hayes K,Sharma L

    更新日期：2015-07-01 00:00:00

  • The effects of oral glucosamine on joint health: is a change in research approach needed?

    abstract：OBJECTIVE:Oral glucosamine (GlcN) has been widely studied for its potential therapeutic benefits in alleviating the pain and disability of osteoarthritis (OA). Its popularity has grown despite ongoing controversy regarding its effectiveness vs placebo in clinical trials, and lack of information regarding possible mecha...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2009.07.005

    authors： Block JA,Oegema TR,Sandy JD,Plaas A

    更新日期：2010-01-01 00:00:00

  • C-reactive protein, metabolic syndrome and incidence of severe hip and knee osteoarthritis. A population-based cohort study.

    abstract：OBJECTIVE:To explore the relationships between C-reactive protein (CRP), metabolic syndrome (MetS) and incidence of severe knee or hip osteoarthritis (OA) in a prospective study. METHODS:A population-based cohort (n=5171, mean age 57.5+/-5.9 years) was examined between 1991 and 1994. Data was collected on lifestyle ha...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2008.07.003

    authors： Engström G,Gerhardsson de Verdier M,Rollof J,Nilsson PM,Lohmander LS

    更新日期：2009-02-01 00:00:00

  • Tribological rehydration of cartilage and its potential role in preserving joint health.

    abstract：OBJECTIVE:During exercise, cartilage recovers interstitial fluid lost during inactivity, which explains how tissue thickness and joint space are maintained over time. This recovery phenomenon is currently explained by a combination of osmotic swelling during intermittent bath exposure and sub-ambient pressurization dur...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.09.018

    authors： Moore AC,Burris DL

    更新日期：2017-01-01 00:00:00

  • The effects of alendronate in the treatment of experimental osteonecrosis of the hip in adult rabbits.

    abstract：OBJECTIVE:Characterize the effects of alendronate (ALN) on the repair process of the osteonecrotic femoral head as well as the development of secondary osteoarthritis in the ipsilateral hip in an established experimental model of osteonecrosis. METHODS:Osteonecrosis of the femoral head was induced surgically in 60 adu...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2008.07.013

    authors： Hofstaetter JG,Wang J,Yan J,Glimcher MJ

    更新日期：2009-03-01 00:00:00

  • Association between KLOTHO gene and hand osteoarthritis in a female Caucasian population.

    abstract：OBJECTIVE:Osteoarthritis (OA) is a common complex disease with strong heritable components. In this study, we investigated the association between four putatively functional genetic variants in KLOTHO gene, a strong ageing-related gene, and hand OA in a large female Caucasian population. METHODS:Subjects (n=1015, age ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2006.12.002

    authors： Zhang F,Zhai G,Kato BS,Hart DJ,Hunter D,Spector TD,Ahmadi KR

    更新日期：2007-06-01 00:00:00

  • Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage.

    abstract：OBJECTIVE:The discovery of small molecular compounds that expand cartilage is needed. We searched for small molecular compounds that expand cartilage or enhance the actions of bone morphogenetic proteins (BMPs) on cartilage. DESIGN:Metatarsal primordial cartilage explants prepared from 14.5 days postcoitum (d.p.c.) mo...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2010.11.007

    authors： Ikegami D,Iwai T,Ryo S,Gu N,Sugiyama T,Oh I,Yoshikawa H,Tsumaki N

    更新日期：2011-02-01 00:00:00

  • The cellular responses of articular cartilage to sharp and blunt trauma.

    abstract：OBJECTIVE:To determine the response of immature articular cartilage to both sharp and blunt trauma in terms of cell death, cell proliferation and matrix synthesis. DESIGN:Blunt wounds were made with a trephine in full depth immature bovine articular cartilage explants which were cut in half through the center of the t...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2002.12.001

    authors： Redman SN,Dowthwaite GP,Thomson BM,Archer CW

    更新日期：2004-02-01 00:00:00

  • Biomechanical and biochemical characteristics of the mandibular condylar cartilage.

    abstract：:The human masticatory system consists of a mandible which is able to move with respect to the skull at its bilateral temporomandibular joint (TMJ) through contractions of the masticatory muscles. Like other synovial joints, the TMJ is loaded mechanically during function. The articular surface of the mandibular condyle...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2009.04.025

    authors： Kuroda S,Tanimoto K,Izawa T,Fujihara S,Koolstra JH,Tanaka E

    更新日期：2009-11-01 00:00:00

  • Hypoxia, RONS and energy metabolism in articular cartilage.

    abstract：OBJECTIVE:Increased pro-inflammatory cytokines and reactive oxygen and nitrogen species (RONS) occur in osteoarthritis (OA). Oxygen tension can alter the levels of RONS induced by interleukin-1 (IL-1). RONS such as nitric oxide (NO) can alter energy metabolism. The aim of this study was to determine if oxygen tension a...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2010.06.004

    authors： Fermor B,Gurumurthy A,Diekman BO

    更新日期：2010-09-01 00:00:00

  • Osteoarthritis year in review 2019: imaging.

    abstract：OBJECTIVE:To provide a narrative review of original articles on osteoarthritis (OA) imaging published between April 1, 2018 and March 30, 2019. METHODS:All original research articles on OA imaging published in English between April 1, 2018 and March 30, 2019 were identified using a PubMed database search. The search t...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章,评审

    doi：10.1016/j.joca.2019.11.009

    authors： Kijowski R,Demehri S,Roemer F,Guermazi A

    更新日期：2020-03-01 00:00:00

  • Mechanism of proteoglycan aggregate degradation in cartilage stimulated with oncostatin M.

    abstract：OBJECTIVE:To investigate the potential synergistic and differential effects of cytokine combinations on proteoglycan aggregate catabolism in cartilage. METHODS:Bovine articular cartilage explants were maintained in organ culture and subjected to stimulation with cytokine combinations including interleukin-1alpha (IL-1...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.05.002

    authors： Durigova M,Roughley PJ,Mort JS

    更新日期：2008-01-01 00:00:00

  • The validity of in vivo ultrasonographic grading of osteoarthritic femoral condylar cartilage: a comparison with in vitro ultrasonographic and histologic gradings.

    abstract：OBJECTIVES:To establish an ultrasonographic (US) grading for semiquantitative evaluation of the femoral condylar cartilage of knee osteoarthritis (OA), in vivo, and compare the in vivo US grading with the in vitro US and histologic gradings. DESIGN:Ninety-five patients going to receive total knee arthroplasty because ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.07.013

    authors： Lee CL,Huang MH,Chai CY,Chen CH,Su JY,Tien YC

    更新日期：2008-03-01 00:00:00

  • Validation of cartilage volume and thickness measurements in the human shoulder with quantitative magnetic resonance imaging.

    abstract：OBJECTIVE:To validate quantitative magnetic resonance imaging (qMRI) for the assessment of cartilage volume and thickness in thin and curved cartilage layers, such as the shoulder. METHODS:Eight shoulder specimens from healthy individuals (aged 31-69 years) were investigated using a 3D gradient echo sequence with sele...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/s1063-4584(03)00077-3

    authors： Graichen H,Jakob J,von Eisenhart-Rothe R,Englmeier KH,Reiser M,Eckstein F

    更新日期：2003-07-01 00:00:00

  • The association between patella alignment and knee pain and function: an MRI study in persons with symptomatic knee osteoarthritis.

    abstract：OBJECTIVE:The aim of the study was to examine the association between patellofemoral (PF) alignment (using standard magnetic resonance imaging (MRI) images of extended knees) and knee pain and function. DESIGN:Subjects were recruited to participate in a natural history study of symptomatic knee osteoarthritis, called ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.04.014

    authors： Kalichman L,Zhu Y,Zhang Y,Niu J,Gale D,Felson DT,Hunter D

    更新日期：2007-11-01 00:00:00

  • Morphological changes of the lateral meniscus in end-stage lateral compartment osteoarthritis of the knee.

    abstract：OBJECTIVE:The aim of this study was to evaluate the morphological changes of the lateral meniscus in end-stage lateral compartment osteoarthritis (OA) of the knee. METHODS:One hundred fifty-eight knee joints from 133 patients that subsequently underwent total knee joint arthroplasty from January 2008 to December 2009 ...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2011.11.005

    authors： Hwang SH,Jung KA,Lee WJ,Yang KH,Lee DW,Carter A,Park CH,Hunter DJ

    更新日期：2012-02-01 00:00:00

  • Reliability, reproducibility and variability of the traditional Histologic/Histochemical Grading System vs the new OARSI Osteoarthritis Cartilage Histopathology Assessment System.

    abstract：OBJECTIVE:For many years, the Histologic/Histochemical Grading System (HHGS) for osteoarthritis monitoring has been used as a histological scoring system for the quality of cartilage. There are, however, some limitations using this grading system. The goal of the investigation presented in this paper was to examine the...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2007.04.017

    authors： Custers RJ,Creemers LB,Verbout AJ,van Rijen MH,Dhert WJ,Saris DB

    更新日期：2007-11-01 00:00:00

  • A dominant TRPV4 variant underlies osteochondrodysplasia in Scottish fold cats.

    abstract：OBJECTIVE:Scottish fold cats, named for their unique ear shape, have a dominantly inherited osteochondrodysplasia involving malformation in the distal forelimbs, distal hindlimbs and tail, and progressive joint destruction. This study aimed to identify the gene and the underlying variant responsible for the osteochondr...

    journal_title：Osteoarthritis and cartilage

    pub_type： 杂志文章

    doi：10.1016/j.joca.2016.03.019

    authors： Gandolfi B,Alamri S,Darby WG,Adhikari B,Lattimer JC,Malik R,Wade CM,Lyons LA,Cheng J,Bateman JF,McIntyre P,Lamandé SR,Haase B

    更新日期：2016-08-01 00:00:00