Managing particulates in cellular therapy.

abstract：BACKGROUND AIMS:Periodontal tissue regeneration with the use of mesenchymal stromal cells (MSCs) has been regarded as a future cell-based therapy. However, low survival rates and the potential tumorigenicity of implanted MSCs could undermine the efficacy of cell-based therapy. The use of conditioned media from MSCs (MS...

journal_title：Cytotherapy

authors： Kawai T,Katagiri W,Osugi M,Sugimura Y,Hibi H,Ueda M

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...

journal_title：Cytotherapy

authors： Picanço V,Heinz S,Bott D,Behrmann M,Covas DT,Seifried E,Tonn T

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND AIMS:Tumor antigen-specific cytotoxic T lymphocytes (CTL) have been used in the treatment of human cancer, including leukemia. Several studies have established PR1 peptide, an HLA-A2.1-restricted peptide derived from proteinase 3 (P3), as a human leukemia-associated antigen. PR1-specific CTL elicited in vitr...

journal_title：Cytotherapy

authors： Ma Q,Wang C,Jones D,Quintanilla KE,Li D,Wang Y,Wieder ED,Clise-Dwyer K,Alatrash G,Mj Y,Munsell MF,Lu S,Qazilbash MH,Molldrem JJ

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Patients with ulcerative colitis (UC) have elevated/activated myeloid lineage leucocytes and may respond favorably to adsorptive granulocyte/monocyte apheresis (GMA). However, there are patients who respond well to GMA, and patients who do not benefit. Therefore, predictive factors of GMA efficacy need to be...

journal_title：Cytotherapy

authors： Yokoyama Y,Watanabe K,Ito H,Nishishita M,Sawada K,Okuyama Y,Okazaki K,Fujii H,Nakase H,Masuda T,Fukunaga K,Andoh A,Nakamura S

更新日期：2015-05-01 00:00:00

abstract：BACKGROUND AIMS:The beneficial effect of human (h) mesenchymal stromal cell (MSC) transplantation in a variety of cell-based intervention strategies is widely believed to be because of paracrine mechanisms. The modification of hMSC cytokine and growth-factor expression patterns were studied following exposure to lipopo...

journal_title：Cytotherapy

authors： Montzka K,Führmann T,Müller-Ehmsen J,Wöltje M,Brook GA

更新日期：2010-11-01 00:00:00

abstract：BACKGROUND AIMS:Although bone marrow c-kit(+) progenitor cells support myocardial regeneration, the cardiomyocyte differentiation potential of umbilical cord blood (UCB) c-kit(+) cells is unknown. METHODS:UCB mononuclear cells (MNCs) and c-kit(+) cells purified by use of immunomagnetic beads were used. Cardiomyocyte d...

journal_title：Cytotherapy

authors： Iachininoto MG,Capodimonti S,Podda MV,Valentini CG,Bianchi M,Leone AM,Teofili L,Leone G

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND AIMS:Adoptive transfer of tumor-infiltrating lymphocytes (TIL) has proven effective in metastatic melanoma and should therefore be explored in other types of cancer. The aim of this study was to examine the feasibility of potentially expanding clinically relevant quantities of tumor-specific T-cell cultures ...

journal_title：Cytotherapy

authors： Junker N,Andersen MH,Wenandy L,Dombernowsky SL,Kiss K,Sørensen CH,Therkildsen MH,Von Buchwald C,Andersen E,Straten PT,Svane IM

更新日期：2011-08-01 00:00:00

abstract：BACKGROUND:Adverse events (AE) represent a significant clinical problem after infusion of cryopreserved HPC. However, the factors playing a role in the pathogenesis have not yet been fully established. METHODS:We prospectively collected data on AE that occurred with 179 HPC infusions performed on patients affected wit...

journal_title：Cytotherapy

authors： Milone G,Mercurio S,Strano A,Leotta S,Pinto V,Battiato K,Coppoletta S,Murgano P,Farsaci B,Privitera A,Giustolisi R

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND AIMS:Traumatic brain injury (TBI) is a leading cause of mortality and morbidity worldwide. Developing effective protocols for the administration of mesenchymal stromal cells (MSCs) is a promising therapeutic strategy to treat TBI. It is important to develop alternatives to direct parenchymal injection at the...

journal_title：Cytotherapy

authors： Bonilla C,Zurita M,Aguayo C,Rodríguez A,Vaquero J

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND AIMS:Cytotherapy is a promising option for neurodegenerative disease treatment. Because of the fatal prognosis and imperative need for effective treatment, amyotrophic lateral sclerosis (ALS) patients request this therapy before its effectiveness has been verified. The increase in clinics offering cytotherap...

journal_title：Cytotherapy

authors： Gamez J,Carmona F,Raguer N,Ferrer-Sancho J,Martín-Henao GA,Martí-Beltrán S,Badia M,Gratacós M,Rodriguez-Gónzalez E,Seoane JL,Pallero-Castillo M,Burgos R,Puiggros C,Pasarin A,Bori-Fortuny I

更新日期：2010-09-01 00:00:00

abstract：BACKGROUND AIMS:Allogeneic hematopoietic stem cell transplantation is curative for sickle cell disease, and the use of matched related donors, non-myeloablative conditioning and sirolimus immunosuppression results in stable mixed chimerism without graft-versus-host disease (GVHD). However, the time to terminate sirolim...

journal_title：Cytotherapy

authors： Raines LN,Hsieh MM,Nassehi T,Drysdale CM,Tisdale JF,Uchida N

更新日期：2019-12-01 00:00:00

abstract：BACKGROUND:The efficiency of chimeric antigen receptor (CAR) T-cell-based therapies depends on a sufficient expansion of CAR T cells in vivo and can be weakened by intra-tumoral suppression of CAR T cell functions, leading to a failure of therapy. For example, certain B-cell malignancies such as chronic lymphocytic leu...

journal_title：Cytotherapy

authors： Štach M,Ptáčková P,Mucha M,Musil J,Klener P,Otáhal P

更新日期：2020-12-01 00:00:00

abstract：BACKGROUND AIMS:In recent years, bone marrow (BM)-derived mesenchymal stromal cells (MSC) have become a promising source for neuroregenerative therapies. We evaluated the trophic effects of neuroectodermally converted MSC (mNSC) on neural stem cells (NSC). METHODS:We quantified the expression of growth factors by mNSC...

journal_title：Cytotherapy

authors： Habisch HJ,Liebau S,Lenk T,Ludolph AC,Brenner R,Storch A

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:Adipose tissue has therapeutic potential for spinal cord injury (SCI) because it contains multipotent cells known as adipose-derived stem/stromal cells (ASCs). In this study, we attempted intravenous ASC transplantation in rats with SCI to examine the effect on functional recovery. METHODS:ASCs (2.5 × ...

journal_title：Cytotherapy

authors： Ohta Y,Hamaguchi A,Ootaki M,Watanabe M,Takeba Y,Iiri T,Matsumoto N,Takenaga M

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) mesenchymal stromal cells (MSC) represent a novel therapy for severe heart failure with extensive myocardial scarring, especially when performed concurrently with conventional revascularization. However, stem cells are difficult to transport in culture media without risk of contaminatio...

journal_title：Cytotherapy

authors： Chin SP,Poey AC,Wong CY,Chang SK,Teh W,Mohr TJ,Cheong SK

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND AIMS:Enumeration of CD34(+) cells in leukocyte-rich cell suspensions is important for clinical decision-making in stem cell transplantation. Single-platform flow cytometry assays offer the significant advantages of speed and reproducibility, and have therefore become the gold standard in stem cell enumeratio...

journal_title：Cytotherapy

authors： Dauber K,Becker D,Odendahl M,Seifried E,Bonig H,Tonn T

更新日期：2011-04-01 00:00:00

abstract：:Regulatory T cells (Tregs) play a fundamental role in the maintenance of self-tolerance and immune homeostasis. Defects in Treg function and/or frequencies have been reported in multiple disease models. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting upper and lower motor neur...

journal_title：Cytotherapy

authors： Alsuliman A,Appel SH,Beers DR,Basar R,Shaim H,Kaur I,Zulovich J,Yvon E,Muftuoglu M,Imahashi N,Kondo K,Liu E,Shpall EJ,Rezvani K

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND AIMS:Previous studies have shown that nicotine increases endothelial progenitor cell (EPC) numbers and functional activity. However, the mechanisms by which nicotine increases EPC numbers and activity remain to be determined. Recent studies have demonstrated that EPC numbers and activity are associated with ...

journal_title：Cytotherapy

authors： Junhui Z,Xiaojing H,Binquan Z,Xudong X,Junzhu C,Guosheng F

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Multiple studies have demonstrated that 'purging' of autografts with 4-hydroperoxycyclophosphamide (4HC) or the related compound mafosfamide (Mf), to eradicate residual leukemia, produces the best results associated with autologous blood and marrow transplantation for AML. However, 4HC purging results in pro...

journal_title：Cytotherapy

authors： McNiece I,Civin C,Harrington J,Kellner J,Malehorn M,Turney J,Barber J,Jones R

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND AIMS:Specific and effective therapy for prevention or reversal of bronchiolitis obliterans (BO) is lacking. In this study, we evaluated the therapeutic effect of hepatocyte growth factor (HGF) gene modified mesenchymal stromal cells (MSCs) on BO. METHODS:A mouse model of experimental BO was established by s...

journal_title：Cytotherapy

authors： Cao XP,Han DM,Zhao L,Guo ZK,Xiao FJ,Zhang YK,Zhang XY,Wang LS,Wang HX,Wang H

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND AIMS:The aim of this study was to evaluate the efficacy and safety of cytokine-induced killer (CIK) cell therapy for solid carcinomas. METHODS:We performed a computerized search of phase II/III clinical trial databases of CIK cell-based therapy using a combination of the terms 'cytokine-induced killer cells...

journal_title：Cytotherapy

authors： Ma Y,Zhang Z,Tang L,Xu YC,Xie ZM,Gu XF,Wang HX

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND AIMS:Endothelial apoptosis underlies the pathophysiology of pulmonary arterial hypertension (PAH). Some factors/cytokines released by endothelial progenitor cells (EPC) have been revealed as potent inhibitors of apoptosis. The aim of this study was to investigate the effects of EPC on pulmonary microvascular...

journal_title：Cytotherapy

authors： Xia L,Fu GS,Yang JX,Zhang FR,Wang XX

更新日期：2009-01-01 00:00:00

abstract：:Despite improved prophylaxis and treatment, GvHD remains a major limitation to optimal allogeneic stem cell transplantation. Ex vivo selective depletion (SD) is a strategy to prevent GvHD, in which host-reactive donor lymphocytes are selectively eliminated from a PBSC allograft while useful donor immune function is pr...

journal_title：Cytotherapy

authors： Mielke S,Solomon SR,Barrett AJ

更新日期：2005-01-01 00:00:00

abstract：:More precise quantitation of cellular immune responses has become possible with the advent of single-cell assays of immune function, such as cytokine flow cytometry, enzyme-linked immunospot (ELISPOT), and MHC-peptide multimers. Cytokine flow cytometry is an attractive technique because it allows the detection of resp...

journal_title：Cytotherapy

authors： Ghanekar SA,Maecker HT

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND AIMS:This study evaluated the feasibility, safety and immunological effects of the intravenous administration of mesenchymal stromal cells (MSCs) from a related donor in patients with refractory aplastic anemia (AA). METHODS:A mean of 6 × 10(5)/kg (range, 5.0-7.1 × 10(5)) MSCs were injected intravenously to...

journal_title：Cytotherapy

authors： Xiao Y,Jiang ZJ,Pang Y,Li L,Gao Y,Xiao HW,Li YH,Zhang H,Liu Q

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND:Traditionally, following high-dose therapy (HDT), unmanipulated autologous PBPC are infused. Alternatively, purified CD34+ cells can now be obtained by immunomagnetic separation using the CliniMACS device. Limited data currently exist examining hemopoietic recovery with such cells. METHODS:Ten patients with...

journal_title：Cytotherapy

authors： Prince HM,Wall D,Rischin D,Toner GC,Seymour JF,Blakey D,Haylock D,Simmons P,Wolf M,Januszewicz EH,Westerman D,Richardson G,Scarlett J,Briggs P

更新日期：2002-01-01 00:00:00

abstract：:A 3-year-old girl with severe aplastic anemia (SAA) that was unresponsive to steroid, cyclosporine and filgrastim treatments received bone marrow (BM) mesenchymal stromal cells (MSC; 1.25 x 10(6)/kg), granulocyte colony-stimulating factor (G-CSF)-mobilized BM and peripheral blood stem cell grafts from her father. Prio...

journal_title：Cytotherapy

authors： Wang H,Wang Z,Xue M,Liu J,Yan H,Guo Z

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:Existing treatments have limited success in modifying the course of peripheral artery disease, which may eventually lead to limb-threatening ulcers and amputation. Cellular therapies have the potential to provide a new treatment option for this condition, but isolation of cells by conventional means has...

journal_title：Cytotherapy

authors： Koch JM,D'Souza SS,Schwahn DJ,Dixon I,Hacker TA

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:[corrected] Recent findings in cord blood (CB) research indicate the potential clinical usefulness of IL-2-activated CB in eradication of minimal malignant residual disease after hematopoietic stem cell transplantation. This feasible approach to immunotherapy merits further pre-clinical investigations using ...

journal_title：Cytotherapy

authors： Derzic S,Slone V,Sender L

更新日期：2005-01-01 00:00:00

abstract：:Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous cultur...

journal_title：Cytotherapy

authors： Huang YZ,Cai JQ,Lv FJ,Xie HL,Yang ZM,Huang YC,Deng L

更新日期：2013-03-01 00:00:00