Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes.

abstract：:What drives scientists' position taking on matters where empirical answers are unavailable or contradictory? We examined the contentious debate on whether to limit experiments involving the creation of potentially pandemic pathogens. Hundreds of scientists, including Nobel laureates, have signed petitions on the debat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edelmann A,Moody J,Light R

更新日期：2017-06-13 00:00:00

abstract：:Two pools of heparan sulfate proteoglycans have been selectively solubilized from rat liver plasma membranes by successive incubations with heparin and detergent. The two populations of proteoglycans have similar polyanionic properties as indicated by identical elution positions on ion-exchange chromatography on DEAE-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kjellén L,Pettersson I,Höök M

更新日期：1981-09-01 00:00:00

abstract：:A central event in the eukaryotic cell cycle is the decision to commence DNA replication (S phase). Strict controls normally operate to prevent repeated rounds of DNA replication without intervening mitoses ("endoreplication") or initiation of mitosis before DNA is fully replicated ("mitotic catastrophe"). Some of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novak B,Tyson JJ

更新日期：1997-08-19 00:00:00

abstract：:In enzyme catalysis, where exquisitely positioned functionality is the sine qua non, atomic coordinates for a Michaelis complex can provide powerful insights into activation of the substrate. We focus here on the initial proton transfer of the isomerization reaction catalyzed by triosephosphate isomerase and present t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jogl G,Rozovsky S,McDermott AE,Tong L

更新日期：2003-01-07 00:00:00

abstract：:The SKW-3 cell line, which was established from the malignant cells of a patient with T-cell chronic lymphocytic leukemia, is characterized by a translocation involving chromosome 8 (band q24) and chromosome 14 (band q11) [t(8;14)(q24;q11)]. To determine the position of the gene encoding the alpha chain of the T-cell ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima EA,Le Beau MM,McKeithan TW,Minowada J,Showe LC,Mak TW,Minden MD,Rowley JD,Diaz MO

更新日期：1986-05-01 00:00:00

abstract：:Aspergillus fumigatus is commonly associated with allergic bronchopulmonary aspergillosis in patients with severe asthma in which chronic airway neutrophilia predicts a poor outcome. We were able to recapitulate fungus-induced neutrophilic airway inflammation in a mouse model in our efforts to understand the underlyin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fei M,Bhatia S,Oriss TB,Yarlagadda M,Khare A,Akira S,Saijo S,Iwakura Y,Fallert Junecko BA,Reinhart TA,Foreman O,Ray P,Kolls J,Ray A

更新日期：2011-03-29 00:00:00

abstract：:Ceramide, a lipid mediator, has been most closely associated with antiproliferative activities. In this study, we examine the mechanism by which ceramide induces growth suppression and the role of the retinoblastoma gene product (Rb) in this process. Withdrawal of serum from the serum-dependent MOLT-4 cells resulted i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dbaibo GS,Pushkareva MY,Jayadev S,Schwarz JK,Horowitz JM,Obeid LM,Hannun YA

更新日期：1995-02-28 00:00:00

abstract：:After intravitreal injection of gamma-[3H] aminobutyric acid (GAB), 2% of the neurons at the outer margin of the inner plexiform layer were intensely labeled. Reconstructions of these neurons from serial electron microscope autoradiograms showed that they are interplexiform cells, which synapse on bipolar processes in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,McGuire BA,Sterling P

更新日期：1980-01-01 00:00:00

abstract：:Two 1,4-benzoquinone derivatives, found in the venom of the scorpion Diplocentrus melici following exposure to air, have been isolated, characterized, synthesized, and assessed for antimicrobial activities. Initially a white, viscous liquid, the extracted venom colors within minutes under ambient conditions. From this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carcamo-Noriega EN,Sathyamoorthi S,Banerjee S,Gnanamani E,Mendoza-Trujillo M,Mata-Espinosa D,Hernández-Pando R,Veytia-Bucheli JI,Possani LD,Zare RN

更新日期：2019-06-25 00:00:00

abstract：:A human beta-globin gene derived from an established human lymphoblast cell line was introduced into murine erythroleukemia (MEL) cells by cell fusion. The globin genes in MEL cells are inducible by dimethyl sulfoxide (Me2SO); induction leads to the accumulation of mouse globin mRNA and hemoglobin. Globin mRNA was not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pyati J,Kucherlapati RS,Skoultchi AI

更新日期：1980-06-01 00:00:00

abstract：:The involvement of epigenetic alterations in mediating effects of estrogens on memory is unknown. The present study determined whether histone acetylation and DNA methylation are critical for the potent estrogen 17beta-estradiol (E(2)) to enhance object recognition memory. We show that dorsal hippocampal E(2) infusion...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Z,Fan L,Frick KM

更新日期：2010-03-23 00:00:00

abstract：:Transgenic crops producing Bacillus thuringiensis (Bt) toxins kill some key insect pests and thus can reduce reliance on insecticides. Widespread planting of such Bt crops increased concerns that their usefulness would be cut short by rapid evolution of resistance to Bt toxins by pests. Pink bollworm (Pectinophora gos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tabashnik BE,Dennehy TJ,Carrière Y

更新日期：2005-10-25 00:00:00

abstract：:Analysis of previously published sets of DNA microarray gene expression data by singular value decomposition has uncovered underlying patterns or "characteristic modes" in their temporal profiles. These patterns contribute unequally to the structure of the expression profiles. Moreover, the essential features of a giv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holter NS,Mitra M,Maritan A,Cieplak M,Banavar JR,Fedoroff NV

更新日期：2000-07-18 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) are a class of cell surface receptors that, upon ligand binding, stimulate a variety of critical cellular functions. The orphan receptor anaplastic lymphoma kinase (ALK) is one of very few RTKs that remain without a firmly established protein ligand. Here we present a novel cytokine, F...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reshetnyak AV,Murray PB,Shi X,Mo ES,Mohanty J,Tome F,Bai H,Gunel M,Lax I,Schlessinger J

更新日期：2015-12-29 00:00:00

abstract：:Cultural processes occur in a wide variety of animal taxa, from insects to cetaceans. The songs of humpback whales are one of the most striking examples of the transmission of a cultural trait and social learning in any nonhuman animal. To understand how songs are learned, we investigate rare cases of song hybridizati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garland EC,Rendell L,Lamoni L,Poole MM,Noad MJ

更新日期：2017-07-25 00:00:00

abstract：:The plant kingdom contains vastly untapped natural product chemistry, which has been traditionally explored through the activity-guided approach. Here, we describe a gene-guided approach to discover and engineer a class of plant ribosomal peptides, the branched cyclic lyciumins. Initially isolated from the Chinese wol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kersten RD,Weng JK

更新日期：2018-11-13 00:00:00

abstract：:Global declines in biodiversity are altering disease dynamics in complex and multifaceted ways. Changes in biodiversity can have several outcomes on disease risk, including dilution and amplification effects, both of which can have a profound influence on the effects of disease in a community. The dilution effect occu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Searle CL,Biga LM,Spatafora JW,Blaustein AR

更新日期：2011-09-27 00:00:00

abstract：:This study uses a multiisotope (carbon, nitrogen, oxygen, and strontium) approach to examine early animal management in the Maya region. An analysis of faunal specimens across almost 2,000 years (1000 BC to AD 950) at the site of Ceibal, Guatemala, reveals the earliest evidence for live-traded dogs and possible captiv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharpe AE,Emery KF,Inomata T,Triadan D,Kamenov GD,Krigbaum J

更新日期：2018-04-03 00:00:00

abstract：:A close study of the metabolic regulation of hexose transport in a hamster fibroblast mutant, highly defective in the enzyme phosphoglucose isomerase (PGI mutant), reveals the requirement for at least three vectors for transport regulation. The downward regulation of the hexose transport system, called the "transport ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalckar HM,Ullrey DB

更新日期：1984-02-01 00:00:00

abstract：:Proteins of plant cell walls serve as structural macromolecules and play important roles in morphogenesis and development but have not been reported to be the origins of peptide signals that activate genes for plant defense. We report here that the mRNA coding the tomato leaf polyprotein precursor of three hydroxyprol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narváez-Vásquez J,Pearce G,Ryan CA

更新日期：2005-09-06 00:00:00

abstract：:Cytokines often deliver simultaneous, yet distinct, cell growth and cell survival signals. The 70-kDa ribosomal protein S6 kinase (p70S6K) is known to regulate cell growth by inducing protein synthesis components. We purified membrane-based p70S6K as a kinase responsible for site-specific phosphorylation of BAD, which...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harada H,Andersen JS,Mann M,Terada N,Korsmeyer SJ

更新日期：2001-08-14 00:00:00

abstract：:The evolutionary process that occurs when a species colonizes a new environment provides an opportunity to explore the mechanisms underlying genetic adaptation, which is essential knowledge for understanding evolution and the maintenance of biodiversity. Atlantic herring has an estimated total breeding stock of about ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill J,Enbody ED,Pettersson ME,Sprehn CG,Bekkevold D,Folkvord A,Laikre L,Kleinau G,Scheerer P,Andersson L

更新日期：2019-09-10 00:00:00

abstract：:The interactions between cancer cells and the surrounding host stromal tissue play a critical role in tumor progression and metastasis, but the molecular nature of this relationship remains largely uncharacterized. Furthermore, although genetic changes of neoplastic cells in tumors contribute significantly to tumor pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pelham RJ,Rodgers L,Hall I,Lucito R,Nguyen KC,Navin N,Hicks J,Mu D,Powers S,Wigler M,Botstein D

更新日期：2006-12-26 00:00:00

abstract：:Immobilization antigens of stock 51 of Paramecium tetraurelia were subjected to electrophoresis in NaDodSO(4)/polyacrylamide gels. Type A is estimated to have a molecular size of 300,000 daltons; H is estimated to be 288,000, D to be 280,000, E to be 270,000, B to be 253,000, and C to be 250,000. Poly(A)(+)RNAs have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Preer JR,Preer LB,Rudman BM

更新日期：1981-11-01 00:00:00

abstract：:X-ray studies on human insulins prepared by semisynthetic and biosynthetic methods have recently been undertaken. Human insulin differs from porcine insulin only at the COOH terminus of the B-chain. The present study reports the crystal structure of 4-zinc human insulin, which is used clinically as a slow-acting prepa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith GD,Swenson DC,Dodson EJ,Dodson GG,Reynolds CD

更新日期：1984-11-01 00:00:00

abstract：:Infection by certain human papillomavirus types is regarded as the major risk factor in the development of cervical cancer, one of the most common cancers of women worldwide. Analysis of the immunogenic and structural features of papillomavirus virions has been hampered by the inability to efficiently propagate the vi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirnbauer R,Booy F,Cheng N,Lowy DR,Schiller JT

更新日期：1992-12-15 00:00:00

abstract：:In autotrophically grown Chlorella cells, glucose induces a hexose transport system but, at the same time, the synthesis of two amino acid transport systems is also induced. Thus, the rates of uptake of glycine, L-alanine, L-proline, and L-serine, all of which compete with each other for entry into the cells, increase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho BH,Sauer N,Komor E,Tanner W

更新日期：1981-06-01 00:00:00

abstract：:Small RNAs (sRNAs) are important components of posttranscriptional regulation. These molecules are prevalent in bacterial and eukaryotic organisms, and involved in a variety of responses to environmental stresses. The functional characterization of sRNAs is challenging and requires highly focused and extensive experim...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Modi SR,Camacho DM,Kohanski MA,Walker GC,Collins JJ

更新日期：2011-09-13 00:00:00

abstract：:Much recent attention in the study of adaptation of organismal form has centered on developmental regulation. As such, the highly conserved respiratory machinery of eukaryotic cells might seem an unlikely target for selection supporting novel morphologies. We demonstrate that a dramatic molecular evolutionary rate inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jobson RW,Nielsen R,Laakkonen L,Wikström M,Albert VA

更新日期：2004-12-28 00:00:00

abstract：:Adrenocortical carcinomas (ACCs) are rare and highly malignant cancers associated with poor survival of patients. Currently, mitotane, a nonspecific derivative of the pesticide DDT (1,1-(dichlorobiphenyl)-2,2-dichloroethane), is used as the standard treatment, but its mechanism of action in ACCs remains elusive. Here ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belavgeni A,Bornstein SR,von Mässenhausen A,Tonnus W,Stumpf J,Meyer C,Othmar E,Latk M,Kanczkowski W,Kroiss M,Hantel C,Hugo C,Fassnacht M,Ziegler CG,Schally AV,Krone NP,Linkermann A

更新日期：2019-10-29 00:00:00