Abstract：
:It has been suggested that the functional diversity of growth hormone (GH) is related to its molecular complexity. Here we report a characterization of charge and mass variants of chicken growth hormone (cGH) through a variety of electrophoretic systems [nondenaturing (ND-PAGE), denaturing (SDS-PAGE), under reducing and nonreducing conditions, isoelectrofocusing (IEF), and bidimensional electrophoresis] followed by Western blot and immunostaining with a specific antibody directed against pure cGH. We also report the biological properties of two charge variants on two homologous assays. The studies were carried out with purified cGH and with fresh chicken pituitary extracts. Three charge variants were obtained by ND-PAGE (Rf = 0.23, 0.30, and 0.35), which showed the same molecular weight (26 kDa), while in IEF eight isoforms were observed, the most conspicuous being those with pI = 6.86, 7.5, 7.9, 8.05, and 8.18. In SDS-PAGE under reducing conditions four immunoreactive bands were observed: the monomer (26 kDa), a dimer (52 kDa), a fragment (16 kDa), and a minor band at 22 kDa. Higher MW variants were found under nonreducing conditions. Bidimensional analysis also showed several charge variants for the monomer and the dimer. Bioactivity of two charge variants (0.23 and 0.3) was evaluated with a lipolytic and an antilipolytic assay on chicken adipose tissue explants. It was shown that variant 0.23 was mainly lipolytic, in a dose-dependent response, but lacked antilipolytic effect. On the other hand, variant 0.30 did not show lipolytic effect but presented a clear antilipolytic activity.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Arámburo C,Montiel JL,Perera G,Navarrete S,Sánchez Rdoi
10.1016/0016-6480(90)90148-fsubject
Has Abstractpub_date
1990-10-01 00:00:00pages
59-67issue
1eissn
0016-6480issn
1095-6840journal_volume
80pub_type
杂志文章abstract：:Previous studies have provided evidence that D-Asp plays a role in steroid-mediated reproductive biology in amphibians, reptiles, birds and mammals. To examine the molecular involvement of D-Asp on steroidogenic pathway regulation, we analysed the expression of StAR, P450 aromatase and 5αRed2 mRNAs in Pelophylax escul...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.10.006
更新日期：2012-01-01 00:00:00
abstract：:Fish have provided essential information about the structure, biosynthesis, evolution, and function of insulin (INS) as well as about the structure, evolution, and mechanism of action of insulin receptors (IR). INS, insulin-like growth factor (IGF)-1, and IGF-2 share a common ancestor; INS and a single IGF occur in Ag...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2011.06.014
更新日期：2011-09-01 00:00:00
abstract：:Endothelin (ET)-1 and suppressor of cytokine signaling (SOCS)-3 were respectively found to regulate energy metabolism and hormone signaling in fat cells. Although ET-1 can also regulate the expression of SOCS-3-stimulating hormones, it is still unknown whether ET-1 regulates SOCS-3 gene expression. This study investig...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.06.024
更新日期：2012-09-15 00:00:00
abstract：:The photoperiodic control of puberty in birds was studied using hybrid red-legged partridges (Alectoris graeca chukar). In the wild partridges first breed at about 10 months of age. Males and females were held from hatch on short (8L:16D) daylengths and then at 3, 4 1/2, 6, 7 1/2, 9, and 12 weeks of age groups were tr...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90290-0
更新日期：1988-07-01 00:00:00
abstract：:The presence of cholecystokinin (CCK), originally isolated from porcine small intestine, has been reported in a diversity of invertebrates ranging, from cnidarians to protochordates, but so far, not in echinoderms. We have used immunohistochemical techniques to demonstrate the presence of cells expressing CCK-like imm...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90026-3
更新日期：1991-08-01 00:00:00
abstract：:Birds face many challenges during seasonal migrations and must make important decisions about whether to accelerate, maintain, or delay travel to their final destinations. Spring migration is likely more challenging than autumn migration as spring journeys are completed more quickly and weather conditions are harsher ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.04.008
更新日期：2019-09-01 00:00:00
abstract：:The neurosecretory caudo-dorsal cells (CDC) of the basommatopora Lymnaea stagnalis produce an ovulation hormone (CDCH), which is released at the periphery of the intercerebral commissure (COM). A rapid in vivo bioassay for CDCH has been established and is based on the dose-dependent relationship between the quantity o...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90269-1
更新日期：1983-06-01 00:00:00
abstract：:Human pro-IGF-I Eb-peptide (hEb) and rainbow trout pro-IGF-I Ea-4-peptide (rtEa-4) have recently been shown to share unique biological activities [Gen. Comp. Endocrinol. 126 (2002) 342; Cell. Exp. Cell. Res. 280 (2002) 75]. To further understand the action mechanism of these proteins, we studied the binding properties...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00089-3
更新日期：2003-06-15 00:00:00
abstract：:Only recently have the cardiac actions of parathyroid hormone and its N-terminal (1-34) fragment (bPTH(1-34] been examined. Parathyroid hormone was found to exert a positive chronotropic effect on the heart tissue of several mammals and one amphibian in vivo and/or in vitro. The purpose of the present study was to exa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90037-6
更新日期：1984-11-01 00:00:00
abstract：:The cell types within the endocrine pancreatic tissue and anterior intestine of larvae and juveniles of representatives of the two southern-hemisphere families (Mordaciidae and Geotriidae) were compared, using immunohistochemistry and antisera against insulin (lamprey, bovine), two somatostatins (SST-14, -34), two PP-...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1152
更新日期：1993-11-01 00:00:00
abstract：:A salmon calcitonin (CT)-like peptide was characterized in various crustaceans by radioimmunological and radioreceptor assays. The highest levels of the molecule were found in the anterior part of the gut and the hepatopancreas of the Norway lobster: Nephrops norvegicus. Molecular sieving of this molecule suggested an...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90163-8
更新日期：1987-02-01 00:00:00
abstract：:Urinary corticosterone metabolite responses to capture have recently been shown for the first time in amphibians, and in the present study urinary corticosterone metabolite responses to capture and to confinement in captivity were measured in adult cane toads (Rhinella marina) in Queensland, Australia. An adrenocortic...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.06.015
更新日期：2011-09-01 00:00:00
abstract：:The tegu lizard Salvator merianae is a large, widely distributed teiid lizard endemic to South America that exhibits annual cycles of high activity during the spring and summer, and hibernation during winter. This pattern of activity and hibernation is accompanied by profound seasonal changes in physiology and behavio...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.06.006
更新日期：2019-03-01 00:00:00
abstract：:Acid extracts of neurointermediate lobe (NIL) and pars distalis (PD) of the pituitary gland of the rainbow trout were radioimmunoassayed for alpha-melanocyte-stimulating hormone (alpha-MSH), beta-melanocyte-stimulating hormone (beta-MSH), adrenocorticotrophin (ACTH), and endorphin. The majority of the alpha-MSH, beta-...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90062-x
更新日期：1983-09-01 00:00:00
abstract：:The skin of Xenopus laevis, because of its high concentrations of amidated peptides, is an excellent model for the study of neuropeptide biosynthesis. In this paper we determined levels of the mRNAs which encode cerulin and characterized the enzymatic activity which likely produces the carboxyl terminal alpha-amide mo...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90206-1
更新日期：1987-07-01 00:00:00
abstract：:Plasma aldosterone concentrations were markedly elevated in chickens by the removal of 10-30% of their total blood volume. This aldosterone response to haemorrhage was delayed and of reduced magnitude in birds pretreated for 48 hr with dexamethasone (100 micrograms/kg every 12 hr), in which the basal aldosterone level...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90315-6
更新日期：1985-11-01 00:00:00
abstract：:Implantation of cortisol pellets in a dorsal lymph sac reduced the frequency with which toads replaced the strata cornea of the epidermis. Except in a few cases, however, the replaced strata cornea were not shed as in a normal moult. The mean interval between replacements of strata cornea rose from about 9-10 days in ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90291-2
更新日期：1988-07-01 00:00:00
abstract：:A rapid and sensitive heterologous enzyme-linked immunosorbent assay (ELISA) was developed to measure rainbow trout maturational gonadotropin. Purified salmon maturational gonadotropin (sGtH II) was used as reference hormone. Optimization of the procedure was performed by using an anti-beta sGtH serum. Two procedures ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90052-n
更新日期：1990-04-01 00:00:00
abstract：:Due to its unique organization, the teleost pituitary is an ideal model in which to investigate the relationship of the nervous system with the pituitary endocrine cells. A light microscope immunocytochemical study of the sea bass pituitary revealed six different neuropeptides in nerve fibers which projected into the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90200-6
更新日期：1988-11-01 00:00:00
abstract：:Follicular development and oocyte recruitment were studied in the ovaries of long-term (2 months) hypophysectomized frogs Rana cyanophlyctis during the postbreeding season (October/November). The effects of homoplastic pituitary pars distalis homogenate (PDH) (1 pituitary per frog), 40 IU pregnant mare serum gonadotro...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90260-6
更新日期：1985-02-01 00:00:00
abstract：:Bovine thyroid-stimulating hormone (TSH) was microinjected (0.2 microliter/fish) into prometamorphic flounder larvae and the effects on metamorphosis as well as the tissue thyroxine (T4) and triiodothyronine (T3) levels were studied. After a single injection of TSH (5 mIU/g), the tissue T4 concentration increased mark...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(89)80038-3
更新日期：1989-06-01 00:00:00
abstract：:Progesterone is believed to have a suppressive or inhibitory role in regulating androgen-dependent sex behaviors in male mammals and birds. Previous studies in this laboratory have revealed that in the little striped whiptail lizard (Cnemidophorus inornatus), progesterone (P) can stimulate sex behavior in a proportion...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90125-4
更新日期：1992-04-01 00:00:00
abstract：:Application of alpha-MSH immunohistochemistry to the brain of elasmobranchs (Scyliorhinus torazame and Etmopterus brachyurus) demonstrated a marked species difference concerning the distribution of the alpha-MSH-like molecule in the brain. In S. torazame, alpha-MSH-like immunoreactive cells were present in the hypotha...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2001.7628
更新日期：2001-06-01 00:00:00
abstract：:Increasing evidence shows that growth hormone (GH) expression is not limited to the pituitary, as it can be produced in many other tissues. It is known that growth hormone (GH) plays a role in the control of reproductive tract development. Acting as an endocrine, paracrine and/or autocrine regulator, GH influences pro...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2014.02.021
更新日期：2014-07-01 00:00:00
abstract：:The aim of present study was to investigate the role of androgen in sperm storage in the female genital tract of Scotophilus heathi. Spermatozoa were observed in the distal part of oviduct and utero-tubal junction of all the female bats collected between January and early March. Increase in circulating testosterone le...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.06.012
更新日期：2010-01-01 00:00:00
abstract：:A gonadotropin-releasing hormone (GnRH) agonist, d-Trp6-LHRH, was evaluated for its effectiveness in reducing circulating testosterone concentrations in wild Hawaiian monk seals (Monachus schauinslandi). Twenty-eight adult male seals were randomly divided into three groups: 10 were captured and treated with 7.5 mg of ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7173
更新日期：1998-11-01 00:00:00
abstract：:Leach's storm-petrels accumulate large amounts of body mass throughout the nestling period. Approximately 4days before fledging, nestlings weigh 50-100% more than adults. In order to shed this excess mass, nestlings engage in behavioral anorexia, and leave the burrow when they are light enough to fly. During this pref...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.07.019
更新日期：2010-10-01 00:00:00
abstract：:Melatonin is a skin lightening agonist of the toad Bufo ictericus. The hormone also exhibits an autodesensitizing action as well as an inhibitory activity on the darkening response to alpha-MSH. In an attempt to pharmacologically characterize the melatonin receptor of the toad pigment cells, serotonin and N-acetylsero...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0110
更新日期：1996-08-01 00:00:00
abstract：:Several of the song control nuclei of songbirds, including HVc (higher vocal center) and Area X, contain melatonin receptor (MelR). In laboratory-housed male starlings, the densities of MelR in Area X change markedly according to reproductive state. MelR are down-regulated when starlings are photostimulated (in full b...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00261-2
更新日期：2003-11-01 00:00:00
abstract：:A highly specific and homologous radioimmunoassay (RIA) for the measurement of prolactin (PRL) in the plasma and the pituitary of the eel was developed using a rabbit antiserum to eel PRL. PRL was purified from the pituitary of Japanese eel (Anguilla japonica). Pituitary extracts and plasma from the Japanese and Europ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90167-5
更新日期：1991-03-01 00:00:00
