Abstract:
:We report the chemical characterization and the relationship between the physicochemical properties and conformational change of a succinoglycan polysaccharide produced by Pseudomonas sp, NCIB 11592. The expected chemical structure is confirmed, with a ratio of D-glucose: D-galactose: pyruvate: succinate of 7:1:1:1. The molecular weight of the native form is 4.2 x 10(6) but after a single heating cycle through the disordered state the molecular weight is reduced to 3.0 x 10(6). The polymer has a polymolecularity index of 1.3 in both cases. The conformational change was studied by different methods which enabled us to define the exact nature of the ordered and disordered states. The conformational transition depends on the temperature, the ionic strength and the nature of the counterion. The polyelectrolyte behaviour is in favour of a single chain conformation with an intramolecular helix-coil transition. The enthalpy change during this transition is greater than that expected solely on the basis of the polyelectrolyte contribution. It may be associated with changes in solvation or a rearrangement of water molecules in close association with the polymer.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Gravanis G,Milas M,Rinaudo M,Clarke-Sturman AJdoi
10.1016/0141-8130(90)90032-6subject
Has Abstractpub_date
1990-06-01 00:00:00pages
195-200issue
3eissn
0141-8130issn
1879-0003pii
0141-8130(90)90032-6journal_volume
12pub_type
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