Abstract：
:In the past decade, Drosophila has emerged as an ideal model organism for studying the genetic components of sleep as well as its regulation and functions. In fruit flies, sleep can be conveniently estimated by measuring the locomotor activity of the flies using techniques and instruments adapted from the field of circadian behavior. However, proper analysis of sleep requires degrees of spatial and temporal resolution higher than is needed by circadian scientists, as well as different algorithms and software for data analysis. Here I describe how to perform sleep experiments in flies using techniques and software (pySolo and pySolo-Video) previously developed in my laboratory. I focus on computer-assisted video tracking to monitor fly activity. I explain how to plan a sleep analysis experiment that covers the basic aspects of sleep, how to prepare the necessary equipment and how to analyze the data. By using this protocol, a typical sleep analysis experiment can be completed in 5-7 d.
journal_name
Nat Protocjournal_title
Nature protocolsauthors
Gilestro GFdoi
10.1038/nprot.2012.041subject
Has Abstractpub_date
2012-04-26 00:00:00pages
995-1007issue
5eissn
1754-2189issn
1750-2799pii
nprot.2012.041journal_volume
7pub_type
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