Abstract：
:The male-specific Y-chromosomal short tandem repeat (STR) is a useful tool in forensic casework. The Y haplotype comprised of 16 loci, which is amplified simultaneously by AmpFlSTR(®) Yfiler(TM) PCR kit and provides strong exculpatory evidence in individual identification. We reported a rare Y-STR profile with a null allele at the DYS448 locus and an off-ladder allele at the DYS456 locus, when genotyping material from a vaginal swab in an alleged rape case. Sequence analysis revealed that the DYS448 null allele was a true type of null allele because of a total deletion of 11 upstream repeats and 9bp of the N(42) region, and there were numerous primer binding site mutations as well. The amplicon of the DYS456 locus was a small 92-bp fragment that was off-ladder, and sequencing analysis showed that there were only 10 repeats (AGAT)(10). This Y chromosome haplotype that was comprised of two variations provided helpful evidence for personal identification.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Ma Y,Kuang JZ,Zhu W,Yang Z,Wang YJ,Nie TG,Li C,Hou YPdoi
10.1111/j.1556-4029.2012.02145.xsubject
Has Abstractpub_date
2012-11-01 00:00:00pages
1630-3issue
6eissn
0022-1198issn
1556-4029journal_volume
57pub_type
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