Abstract：
:Accurate quantification of DNA samples is an important step in obtaining accurate and reproducible short tandem repeat (STR) profiles. Quantitative real-time-PCR has improved the speed and accuracy of DNA quantification over earlier methods, albeit at significantly greater cost per reaction. Here, the performance of reduced volume (10 microL) DNA quantification assays using the Quantifiler Human DNA Quantification Kit was evaluated using commercial standards and single source biological stains (e.g., venous blood, saliva, and semen). In addition, casework-type samples including those subjected to environmental contaminants containing PCR inhibitors and samples having undergone extensive DNA degradation were also quantified. The concentration of DNA in various forensic samples ranged from 0 to 2.9 ng/microL depending on sample source and/or environmental insult. Compared to full-scale reactions, reduced volume assays displayed equivalent to improved amplification efficiency and sample-to-sample reproducibility (+/-0.01-0.17 C(T FAM)). Furthermore, the use of data from reduced-scale Quantifiler reactions facilitated the accurate determination of the amount of sample DNA extract needed to generate quality STR profiles. The use of 10 microL-scale Quantifiler reaction volumes has the practical benefit of increasing the effective number of reactions per kit by 250%; thereby reducing the cost per assay by 60% while consuming less sample. This is particularly advantageous in cases of consumptive testing.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Westring CG,Kristinsson R,Gilbert DM,Danielson PBdoi
10.1111/j.1556-4029.2007.00525.xsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
1035-43issue
5eissn
0022-1198issn
1556-4029pii
JFO525journal_volume
52pub_type
杂志文章abstract：:The main disadvantage to gunshot residue (GSR) particle analysis utilizing scanning electron microscope/energy dispersive X-ray (SEM/EDX) instrumentation has been the excessive operator time required for search and identification. This study uses an automated particle search and characterization program for unattended...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Allele frequencies for the locus D1S80 were determined in African American, Caucasian, Southeastern Hispanic, Southwestern Hispanic, and Oriental sample populations using the polymerase chain reaction and subsequent electrophoresis and silver staining of the amplified products. Due to the presence of anodal and cathod...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:A method to use ionizing radiation to inactivate HIV (Human Immunodeficiency Virus) in human body fluids was studied in an effort to reduce the risk of accidental infection to forensic science laboratory workers. Experiments conducted indicate that an X-ray absorbed dose of 25 krad was required to completely inactivat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:The term Aqua-eroticum was first introduced in 1984 by Sivaloganathan to describe the unusual autoerotic death of a man using submersion as an asphyxia method. This was the first case of that kind, and since then, no other case of autoerotic submersion has been reported, nor other autoerotic fatality in open water. He...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2005.00031.x
更新日期：2006-01-01 00:00:00
abstract：:The separation of standard opiates in a mixture and their analysis in clandestine and pharmaceutical preparations were accomplished by PIC on a micro-Bondapak C18 column. The identification of the opiates was based on two parameters: retention times and the ratios of absorbance peaks recorded at 254 and 280 nm. No pri...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
abstract：:To estimate postmortem interval (PMI), spontaneous cleavage of the third component of complement (C3) was studied in aged blood and cadaveric blood by crossed immunoelectrophoresis. Using the kinetics of C3 cleavage in vitro described as dC/dt = -kC, where C is the concentration of native C3 at time t and k is a first...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Estimating the time since death of the deceased is a main goal in forensic investigations, but this can be challenging due to contradictory results derived from different investigations at the scene of death. We present a case of a 78-year-old woman, found dead in a small forest with broad-leaved trees, whose husband ...
journal_title：Journal of forensic sciences
pub_type：
doi：10.1111/1556-4029.14010
更新日期：2019-09-01 00:00:00
abstract：:The rising incidence of assaults involving lachrymator sprays has led to an increase in items being submitted to this laboratory for the analysis of the associated chemical residues. The following work was undertaken to identify an efficient solvent with which to extract the compounds of interest from cotton fabric. T...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:In bloodstain pattern analysis, the blood droplet volume and surface impact velocity play an important role, and many related experimental studies have been carried out. If an appropriate computational fluid dynamics (CFD) model that could solve bloodstain patterns, especially spine formation bloodstain patterns, can ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12484
更新日期：2014-11-01 00:00:00
abstract：:An efficient extraction and gas chromatography/mass spectrometry (GC/MS) procedure has been developed for the simultaneous determination of methadone and 2-ethyl-1,5-dimethyl-3,3-diphenylpyrrolidine in urine samples. The merits of this procedure include (1) effective high-volume sample processing; (2) excellent gas ch...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:Measurements of the type and concentration of propellant and stabilizer additives in smokeless gunpowder are used by forensic scientists investigating the source of explosives and by military laboratories assuring the safety and efficacy of munitions. The National Institute of Standards and Technology recently assesse...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:The identification, analysis, and occurrence in U.S. automobile original finishes (1974-1989) of Nickel Titanate (yellow) and Chrome Titanate (yellow-orange) are described in this report. The titanate pigments are based on the rutile (titanium dioxide) structure and there are only minor differences between the infrare...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00126.x
更新日期：2006-05-01 00:00:00
abstract：:A considerable amount of discussion can be found in the forensics literature about the issue of using statistical sampling to obtain for chemical analyses an appropriate subset of units from a police seizure suspected to contain illicit material. Use of the Bayesian paradigm has been suggested as the most suitable sta...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01930.x
更新日期：2012-01-01 00:00:00
abstract：:The Gustafson technique was a significant contribution to forensic identification. Unfortunately, many statistical errors were present in the published articles. It now seems clear that greater precision can be achieved through modification of the original technique. One of the authors (W. R. M.) will report soon on m...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Potassium chloride intravenous injection is used in suicide attempts and lethal procedures for state-sanctioned punishment. Owing to its relatively high concentrations in hemolyzed blood (25-80 mM) compared to serum (about 4 mM), it is difficult to conclude potassium poisoning by postmortem analysis of biologic sample...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01907.x
更新日期：2012-01-01 00:00:00
abstract：:This paternity study was performed with trios in which the putative father was not the biological father (NBF), in order to evaluate the effectiveness of HLA-A and -B phenotyping for routine testing. All 372 generated trios had ABO, Rh, MNS, Kell, Duffy, Kidd and HLA (A and B) systems tested. HLA-A and -B phenotyping ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:Short tandem repeat (STR) loci represent a rich source of highly polymorphic markers in the human genome which are useful for the purposes of forensic identification and determination of biological relatedness of individuals. Here, as a part of an ongoing extensive study, we report the analysis of a multilocus genotyp...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:To understand how time and temperature could affect latent fingerprints, wooden pallets were used to construct five units that each housed ten 60-watt incandescent light bulbs. Fingerprints were deposited on the top, middle, and base of the globes. The bulbs were powered on (except the control bulb in each unit) for 1...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14133
更新日期：2020-01-01 00:00:00
abstract：:A 5-year study of a busy ski population in Utah revealed a total at-risk period of approximately 3.5 million skier-days. While the morbidity (fractures, strains, sprains, and occasionally more severe injuries) associated with skiing is well recognized and considered by most physicians concerned to be distributed somew...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:In postmortem drug analysis, the most commonly used sample matrix is whole blood. However, postmortem changes can denature this matrix, resulting in a loss or degradation of drugs, thus biasing analytical findings. Vitreous humor is thought to be less affected by these changes and should, therefore, have the potential...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:Sodium and potassium cyanide are highly toxic, produced in large amounts by the chemical industry, and linked to numerous high-profile crimes. The U.S. Centers for Disease Control and Prevention has identified cyanide as one of the most probable agents to be used in a chemical terrorism event. We investigated whether ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2011.01946.x
更新日期：2012-01-01 00:00:00
abstract：:Among the methods dealing with the age estimation, the evaluation of the ratio of the D-, L- form of the aspartic acid in tissues with a low metabolic turnover is considered to be the most precise. We introduced demineralization of the dentin with 0.5 M EDTA adjusted to pH = 7.4. The advantage of such a procedure is t...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Despite technological advances, human remains detection (HRD) dogs still remain one of the best tools for locating clandestine graves. However, soil texture may affect the escape of decomposition gases and therefore the effectiveness of HDR dogs. Six nationally credentialed HRD dogs (three HRD only and three cross-tra...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13084
更新日期：2016-05-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01508.x
更新日期：2011-01-01 00:00:00
abstract：:Postmortem computed tomography (PMCT) is a relatively recent advancement in forensic pathology practice that has been increasingly used as an ancillary investigation and screening tool. One area of clinical CT imaging that has garnered a lot of research interest recently is the area of "artificial intelligence" (AI), ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14502
更新日期：2020-11-01 00:00:00
abstract：:Both among psychiatric inpatients and inmates of prisons and jails, suicide is highly prevalent with alarming rates. In many countries, there has been a call for action to prevent such deaths and to educate staff in the early recognition of suicide risk. A careful MedLine search was used to identify relevant papers de...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：10.1111/j.1556-4029.2009.01122.x
更新日期：2009-09-01 00:00:00
abstract：:Mobile multi-detector computed tomography (MDCT) scanners are potentially available to temporary mortuaries and can be operational within 20 min of arrival. We describe, to our knowledge, the first use of mobile MDCT for a mass fatality incident. A mobile MDCT scanner attended the disaster mortuary after a five vehicl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00548.x
更新日期：2007-11-01 00:00:00
abstract：:We tested a new approach to the stature prediction that could be used in the identification process of human skeletal remains of unknown identity. The stature of 19 female and 21 male adult cadavers was measured within 24 h after death and considered to be equal to the living stature. The antero-posterior radiographs ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00419.x
更新日期：2007-05-01 00:00:00
abstract：:Blood samples of two cases were analyzed preliminarily by a classical spectrophotometric method (VIS) and by an automated headspace gas chromatographic method with nitrogen-phosphorus detector (HS-GC/NPD). In the former, hydrogen cyanide (HCN) was quantitatively determined by measuring the absorbance of chromophores f...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00570.x
更新日期：2007-11-01 00:00:00
abstract：:DNA was isolated from casework urine samples previously submitted for toxicological analysis. The quality and quantity of DNA isolated was determined by spectrofluorometry and agarose yield gel electrophoresis. Hae III restricted samples were then resolved by analytical agarose gel electrophoresis, transferred to a me...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
