Crystal structure of Staphylococcus aureus transglycosylase in complex with a lipid II analog and elucidation of peptidoglycan synthesis mechanism.

abstract：:Assemblies formed by solid particles at interfaces have been widely studied because they serve as models of molecular phenomena, including molecular self-assembly. Solid particles adsorbed at interfaces also provide a means of stabilizing liquid-liquid emulsions and synthesizing materials with tunable mechanical, opti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koenig GM Jr,Lin IH,Abbott NL

更新日期：2010-03-02 00:00:00

abstract：:A high-resolution compositional map of glass-forming ability (GFA) in the Ni-Cr-Nb-P-B system is experimentally determined along various compositional planes. GFA is shown to be a piecewise continuous function formed by intersecting compositional subsurfaces, each associated with a nucleation pathway for a specific cr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Na JH,Demetriou MD,Floyd M,Hoff A,Garrett GR,Johnson WL

更新日期：2014-06-24 00:00:00

abstract：:We tested the influence of a photothrombotic lesion in somatosensory cortex on plasticity in the mouse visual system and the efficacy of anti-inflammatory treatment to rescue compromised learning. To challenge plasticity mechanisms, we induced monocular deprivation (MD) in 3-mo-old mice. In control animals, MD induced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greifzu F,Schmidt S,Schmidt KF,Kreikemeier K,Witte OW,Löwel S

更新日期：2011-09-13 00:00:00

abstract：:With current concerns of antibiotic-resistant bacteria and biodefense, it has become important to rapidly identify infectious bacteria. Traditional technologies involving isolation and amplification of the pathogenic bacteria are time-consuming. We report a rapid and simple method that combines in vivo biotinylation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edgar R,McKinstry M,Hwang J,Oppenheim AB,Fekete RA,Giulian G,Merril C,Nagashima K,Adhya S

更新日期：2006-03-28 00:00:00

abstract：:Previous studies have identified lipid-poor high density lipoproteins with electrophoretic pre-beta mobility as the initial acceptors of cell-derived cholesterol in human plasma. These lipoproteins contain apolipoprotein A-I (apo A-I) as their sole apolipoprotein. In the present study, incubation of human plasma with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang Y,von Eckardstein A,Wu S,Maeda N,Assmann G

更新日期：1994-03-01 00:00:00

abstract：:The carbon budget and dynamics of the Earth's interior, including the core, are currently very poorly understood. Diamond-bearing, mantle-derived rocks show a very well defined peak at δ(13)C ≈ -5 ± 3‰ with a very broad distribution to lower values (∼-40‰). The processes that have produced the wide δ(13)C distribution...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horita J,Polyakov VB

更新日期：2015-01-06 00:00:00

abstract：:Plasma samples exhibiting alloalbuminemia on electrophoresis at pH 8.6 were requested from clinical laboratories throughout Sweden. Nine variants, each representing a different single point mutation, were found in 100 apparently unrelated Swedes. The overall prevalence of alloalbuminemia was estimated at 1:1700. Mutat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson J,Sakamoto Y,Laurell CB,Madison J,Watkins S,Putnam FW

更新日期：1992-09-01 00:00:00

abstract：:Light microscopy autoradiography has been used to visualize neuroanatomical patterns of brain opiate receptor upregulation in response to chronic naltrexone administration. Slide-mounted brain sections of frozen rat brain were labeled in vitro with dihydro[3H]morphine, a relatively selective mu opioid ligand. The grea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tempel A,Gardner EL,Zukin RS

更新日期：1984-06-01 00:00:00

abstract：:Human P-glycoprotein (Pgp) confers multidrug resistance to cancer cells by ATP-dependent extrusion of a great many structurally dissimilar hydrophobic compounds. The manner in which Pgp recognizes these different substrates is unknown. The protein shows internal homology between its N- and C-terminal halves, each comp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dey S,Ramachandra M,Pastan I,Gottesman MM,Ambudkar SV

更新日期：1997-09-30 00:00:00

abstract：:We developed a method for rapidly generating thermostable enzyme variants. Our strategy is to introduce the gene coding for a given enzyme from a mesophilic organism into a thermophile, Bacillus stearothermophilus. Variants that retain the enzymatic activity at the higher growth temperatures of the thermophile are the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao H,McKenzie T,Hageman R

更新日期：1986-02-01 00:00:00

abstract：:Treatment of C57BL/6 or NOD mice with a monoclonal antibody to the CSF-1 receptor resulted in depletion of the resident macrophages of pancreatic islets of Langerhans that lasted for several weeks. Depletion of macrophages in C57BL/6 mice did not affect multiple parameters of islet function, including glucose response...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrero JA,McCarthy DP,Ferris ST,Wan X,Hu H,Zinselmeyer BH,Vomund AN,Unanue ER

更新日期：2017-11-28 00:00:00

abstract：:The maximum rate of expression of the lac operon is severely reduced in lac promoter mutants. Revertants of these mutations which produce higher levels of enzyme were isolated. Some of these revertants had lost sensitivity to catabolite repression and transient repression. The mutations responsible for these losses to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Silverstone AE,Arditti RR,Magasanik B

更新日期：1970-07-01 00:00:00

abstract：:This study demonstrates the presence of an N-acetyl-D-galactosaminyltransferase in human serum and in erythrocyte membranes. This enzyme catalyzes the transfer of N-acetyl-D-galactosamine from UDP-N-acetyl-D-galactosamine to a mucin receptor and 2'-fucosyllactose that have blood group H activity and may be responsible...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim YS,Perdomo J,Bella A Jr,Nordberg J

更新日期：1971-08-01 00:00:00

abstract：:We have found that 1 of 20 human tumors transplanted and passaged in nude mice was associated with a massive induction of endogenous murine leukemia virus (MuLV). Separation and growth of these viruses on various substrates indicated that both ecotropic and xenotropic MuLV were present in the induced mixture. Tryptic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gautsch JW,Knowles AF,Jensen FC,Kaplan NO

更新日期：1980-04-01 00:00:00

abstract：:Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmt1p; EC 2.3.1.97) is an essential enzyme that is highly selective for myristoyl-CoA in vivo. It is unclear why myristate (C14:0), a rare cellular fatty acid, has been selected for this covalent protein modification over more abundant fatty acids ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rudnick DA,Lu T,Jackson-Machelski E,Hernandez JC,Li Q,Gokel GW,Gordon JI

更新日期：1992-11-01 00:00:00

abstract：:Autocatalytic activation of an initiator caspase triggers the onset of apoptosis. In dying cells, caspase-9 activation is mediated by a multimeric adaptor complex known as the Apaf-1 apoptosome. The molecular mechanism by which caspase-9 is activated by the Apaf-1 apoptosome remains largely unknown. Here we demonstrat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Q,Wu D,Chen W,Yan Z,Yan C,He T,Liang Q,Shi Y

更新日期：2014-11-18 00:00:00

abstract：:We show that the stimulation of human immunodeficiency virus (HIV) brought about by tumor necrosis factor alpha and phorbol 12-myristate 13-acetate can be inhibited by adding N-acetyl-L-cysteine (NAC). NAC, which replenishes intracellular glutathione, effectively inhibits the tumor necrosis factor alpha- or phorbol es...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roederer M,Staal FJ,Raju PA,Ela SW,Herzenberg LA,Herzenberg LA

更新日期：1990-06-01 00:00:00

abstract：:The interrelationships of hexose feeding and insulin action were studied in the Chinese hamster fibroblast cell lines 023 and DS-7. The latter, derived from 023 and deficient in phosphoglucose isomerase, has been used to map the metabolic requirements for aldohexose-mediated down-regulation or "curbing" of hexose tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson C,Peterson SW,Ullrey DB,Kalckar HM

更新日期：1986-06-01 00:00:00

abstract：:The problem of the propagation of conformational changes over long distances or through a closely packed protein is shown to fit a model of a ligand-induced conformational change between two protein states selected by evolution. Moreover, the kinetics of the pathway between these states is also selected so that the en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu EW,Koshland DE Jr

更新日期：2001-08-14 00:00:00

abstract：:Fragile X syndrome (FXS) results in intellectual disability (ID) most often caused by silencing of the fragile X mental retardation 1 (FMR1) gene. The resulting absence of fragile X mental retardation protein 1 (FMRP) leads to both pre- and postsynaptic defects, yet whether the pre- and postsynaptic functions of FMRP ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myrick LK,Deng PY,Hashimoto H,Oh YM,Cho Y,Poidevin MJ,Suhl JA,Visootsak J,Cavalli V,Jin P,Cheng X,Warren ST,Klyachko VA

更新日期：2015-01-27 00:00:00

abstract：:The water channel aquaporin 1 (AQP1) and certain Rh-family members are permeable to CO(2) and NH(3). Here, we use changes in surface pH (pH(S)) to assess relative CO(2) vs. NH(3) permeability of Xenopus oocytes expressing members of the AQP or Rh family. Exposed to CO(2) or NH(3), AQP1 oocytes exhibit a greater maxima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musa-Aziz R,Chen LM,Pelletier MF,Boron WF

更新日期：2009-03-31 00:00:00

abstract：:The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoover RL,Karnovsky MJ,Austen KF,Corey EJ,Lewis RA

更新日期：1984-04-01 00:00:00

abstract：:The segmented body plan of vertebrates is prefigured by reiterated embryonic mesodermal structures called somites. In the mouse embryo, timely somite formation from the presomitic mesoderm (PSM) is controlled by the "segmentation clock," a molecular oscillator that triggers progressive waves of Notch activity througho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopez TP,Fan CM

更新日期：2013-05-28 00:00:00

abstract：:Understanding biological and physical processes involving nucleic acids, such as the binding of proteins to DNA and RNA, DNA condensation, and RNA folding, requires an understanding of the ion atmosphere that surrounds nucleic acids. We have used a simple model DNA system to determine how the ion atmosphere modulates ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bai Y,Das R,Millett IS,Herschlag D,Doniach S

更新日期：2005-01-25 00:00:00

abstract：:Opportunistic infections are often polymicrobial. Two of the most important bacterial opportunistic pathogens of humans, Pseudomonas aeruginosa and Staphylococcus aureus, frequently are coisolated from infections of catheters, endotracheal tubes, skin, eyes, and the respiratory tract, including the airways of people w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman LR,Déziel E,D'Argenio DA,Lépine F,Emerson J,McNamara S,Gibson RL,Ramsey BW,Miller SI

更新日期：2006-12-26 00:00:00

abstract：:We have attempted to model human metastatic disease by implanting human target organs into the immunodeficient C.B-17 scid/scid (severe combined immunodeficiency; SCID) mouse, creating SCID-hu mice. Preferential metastasis to implants of human fetal lung and human fetal bone marrow occurred after i.v. injection of hum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shtivelman E,Namikawa R

更新日期：1995-05-09 00:00:00

abstract：:A recent major conceptual advance has been the recognition of the importance of immune system-neuronal interactions in the modulation of brain function, one example of which is spinal pain processing in neuropathic states. Here, we report that in peripheral nerve-injured rats, the lysosomal cysteine protease cathepsin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark AK,Yip PK,Grist J,Gentry C,Staniland AA,Marchand F,Dehvari M,Wotherspoon G,Winter J,Ullah J,Bevan S,Malcangio M

更新日期：2007-06-19 00:00:00

abstract：:The POT1 (protection of telomeres 1) protein binds the ssDNA overhangs at the ends of chromosomes in diverse eukaryotes. POT1 is essential for chromosome end-protection, as best demonstrated in fission yeast. In human cells, hPOT1 is also involved in telomere-length regulation. We now show that telomeric oligonucleoti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaug AJ,Podell ER,Cech TR

更新日期：2005-08-02 00:00:00

abstract：:The CH family of murine B-cell lymphomas includes several members that are sensitive to growth inhibition when their membrane IgM (mIgM) receptors are cross-linked by anti-mu chain, anti-kappa chain, or anti-idiotypic antibodies. These lymphomas are IgM+, Ia+, and IgD +/- and resemble neonatal B cells in terms of thei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alés-Martínez JE,Warner GL,Scott DW

更新日期：1988-09-01 00:00:00

abstract：:Deuterium oxide, D(2)O, increases the temperature-tolerance of Drosophila pseudoobscura when it is administered to adult flies as a sucrose solution. The effect is very rapidly exerted; it is detected within 10 min after the flies have a brief (10 min) opportunity to drink. This increased resistance to heat-death sure...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pittendrigh CS,Cosbey ES

更新日期：1974-02-01 00:00:00