Quantitative assessment of ultrastructure and light scatter in mouse corneal debridement wounds.

abstract：PURPOSE:To assess the impact of a novel visual field spatial filtering technique on the detection of glaucomatous progression. METHODS:One hundred ninety-eight ocular hypertensive (OHT) and 21 control subjects were examined prospectively (1994-2001) with regular full-threshold Humphrey visual field (VF) testing. VF pr...

journal_title：Investigative ophthalmology & visual science

authors： Strouthidis NG,Scott A,Viswanathan AC,Crabb DP,Garway-Heath DF

更新日期：2007-01-01 00:00:00

abstract：PURPOSE:The purpose of this study was to identify target antigens of autoimmune keratitis with a disease-prone mouse model. METHODS:BALB/c nude mice grafted with embryonic rat thymi (TG nude mice) develop various organ-localized autoimmune lesions, including keratitis. A hybridoma producing a monoclonal antibody (OT-2...

journal_title：Investigative ophthalmology & visual science

authors： Hattori T,Takeuchi M,Ohno K,Ogawara M,Asatani T,Usui Y,Muramatsu R,Inagaki M,Usui M,Taguchi O

更新日期：2006-01-01 00:00:00

abstract：:The binding of plasma fibronectin to the collagens I, II, and III was greater in a cohort of glaucoma patients. In contrast, binding of plasma fibronectin to collagen type IV was less in 39 glaucoma patients than in any of 3 other diagnostic categories, including 92 patients that were normal, cataract, and glaucoma su...

journal_title：Investigative ophthalmology & visual science

authors： Worthen DM,Cleveland PH,Slight JR,Abare J

更新日期：1985-12-01 00:00:00

abstract：PURPOSE:The objective of the study was to investigate the effect of glial cell line-derived neurotrophic factor (GDNF) on the multifocal electroretinogram (mfERG) following an induced branch retinal vein occlusion (BRVO) in pigs. METHODS:Electrophysiological examination of the retina was performed in 20 pigs with stan...

journal_title：Investigative ophthalmology & visual science

authors： Ejstrup R,Cour Ml,Kyhn MV,Heegaard S,Kiilgaard JF

更新日期：2012-09-14 00:00:00

abstract：Purpose:Diabetes leads to the downregulation of the retinal Kir4.1 channels and Müller cell dysfunction. The insulin receptor substrate-1 (IRS-1) is a critical regulator of insulin signaling in Müller cells. Circadian rhythms play an integral role in normal physiology; however, diabetes leads to a circadian dysrhythmia...

journal_title：Investigative ophthalmology & visual science

authors： Luo Q,Xiao Y,Alex A,Cummins TR,Bhatwadekar AD

更新日期：2019-05-01 00:00:00

abstract：PURPOSE:Although primary congenital glaucoma (PCG)-associated CYP1B1 mutations in the heterozygous state have been evaluated for association with primary open-angle glaucoma (POAG) in several small studies, their contribution to the occurrence of POAG is still controversial. The present study was conducted to determine...

journal_title：Investigative ophthalmology & visual science

authors： Pasutto F,Chavarria-Soley G,Mardin CY,Michels-Rautenstrauss K,Ingelman-Sundberg M,Fernández-Martínez L,Weber BH,Rautenstrauss B,Reis A

更新日期：2010-01-01 00:00:00

abstract：PURPOSE:To determine the efficacy of a new formulation of topical dexamethasone 0.1%/povidone-iodine 0.4% (FST-100) in reducing clinical symptoms and infectious viral titers in a rabbit model of adenoviral keratoconjunctivitis. METHODS:Rabbit corneas were inoculated bilaterally with 2×10(6) plaque-forming-units (PFU) ...

journal_title：Investigative ophthalmology & visual science

authors： Clement C,Capriotti JA,Kumar M,Hobden JA,Foster TP,Bhattacharjee PS,Thompson HW,Mahmud R,Liang B,Hill JM

更新日期：2011-01-21 00:00:00

abstract：PURPOSE:To identify the differently expressed micro (mi) RNAs in pterygium compared with normal conjunctiva and investigate the potential role of miRNAs in the pathogenesis of pterygium. METHODS:With microRNA microarray and quantitative RT-PCR, we identified that microRNA-122 (miR-122) was significantly decreased in p...

journal_title：Investigative ophthalmology & visual science

authors： Cui YH,Li HY,Gao ZX,Liang N,Ma SS,Meng FJ,Li ZJ,Pan HW

更新日期：2016-07-01 00:00:00

abstract：:Plasminogen activator (PA) activity during epithelial wound healing in vitamin A-deficient rats was investigated to determine whether a relationship exists between corneal defect formation and PA activity. Uniform, central 3 mm diameter corneal epithelial wounds were made by scalpel debridement in vitamin A-deficient ...

journal_title：Investigative ophthalmology & visual science

authors： Hayashi K,Frangieh G,Kenyon KR,Berman M,Wolf G

更新日期：1988-12-01 00:00:00

abstract：Purpose:The purpose of this study was to study the status and association among tear-soluble factors, corneal dendritic cell density, vitamin D, and signs and symptoms in dry eye disease (DED). Methods:A total of 33 control subjects and 47 evaporative dry eye patients were included in the study. DED diagnosis and clas...

journal_title：Investigative ophthalmology & visual science

authors： Khamar P,Nair AP,Shetty R,Vaidya T,Subramani M,Ponnalagu M,Dhamodaran K,D'souza S,Ghosh A,Pahuja N,Deshmukh R,Ahuja P,Sainani K,Nuijts RMMA,Das D,Ghosh A,Sethu S

更新日期：2019-06-03 00:00:00

abstract：PURPOSE:The purpose of the present study was to examine the roles of signaling pathways potentially activated by TGFbeta (i.e., Smad and p38 mitogen-activated kinase [MAPK]) in regulation of cell migration and proliferation of healing mouse corneal epithelium. METHODS:Activation of Smads or p38MAPK was evaluated by im...

journal_title：Investigative ophthalmology & visual science

authors： Saika S,Okada Y,Miyamoto T,Yamanaka O,Ohnishi Y,Ooshima A,Liu CY,Weng D,Kao WW

更新日期：2004-01-01 00:00:00

abstract：PURPOSE:We investigated the phenotype of macrophages infiltrating rejected corneal allografts. METHODS:We performed allogeneic or syngeneic corneal transplantation in mice, and humanely killed animals at day 28 during allograft rejection when 60% of corneal allografts were rejected. We divided allografts into two grou...

journal_title：Investigative ophthalmology & visual science

authors： Oh JY,Lee HJ,Ko AY,Ko JH,Kim MK,Wee WR

更新日期：2013-11-21 00:00:00

abstract：PURPOSE:To investigate thickness profile changes of the corneal epithelium and Bowman's layer at the vertical and horizontal meridians with overnight myopia orthokeratology (OK) lenses. METHODS:Twenty subjects (age range: 19-33 years) wore reverse-geometry rigid gas-permeable OK lenses in both eyes for 30 days. Before...

journal_title：Investigative ophthalmology & visual science

authors： Lian Y,Shen M,Jiang J,Mao X,Lu P,Zhu D,Chen Q,Wang J,Lu F

更新日期：2013-01-23 00:00:00

abstract：PURPOSE:Transforming growth factor beta-induced (TGFBI)-related dystrophies constitute the most common heritable forms of corneal dystrophy worldwide. However, other than the underlying genotypes of these conditions, a limited knowledge exists of the exact pathomechanisms of these disorders. This study expands on our p...

journal_title：Investigative ophthalmology & visual science

authors： Courtney DG,Poulsen ET,Kennedy S,Moore JE,Atkinson SD,Maurizi E,Nesbit MA,Moore CB,Enghild JJ

更新日期：2015-07-01 00:00:00

abstract：PURPOSE:The goal was to establish an accurate method for measuring intraocular pressure (IOP) in living adult zebrafish and to determine the average IOPs of genetically different strains and mutants. The overall purpose was to develop the zebrafish system for studies of IOP and glaucoma. Elevated IOP and anterior segme...

journal_title：Investigative ophthalmology & visual science

authors： Link BA,Gray MP,Smith RS,John SW

更新日期：2004-12-01 00:00:00

abstract：PURPOSE:The purpose of the present study was to investigate the roles of caspase 1 and extracellular signal-regulated kinase (ERK) in inflammation-induced inhibition of lacrimal gland secretion. METHODS:Lacrimal gland inflammation was induced by injection of lipopolysaccharide (LPS; to study the role of caspase 1) or ...

journal_title：Investigative ophthalmology & visual science

authors： Zoukhri D,Ko S,Stark PC,Kublin CL

更新日期：2008-10-01 00:00:00

abstract：Purpose:To use laser speckle flowgraphy (LSFG) to assess blood flow (BF) in the optic nerve head (ONH) tissue and choroid during elevated intraocular pressure (IOP). Methods:This prospective study included 20 eyes of 20 healthy volunteers. The testing protocol had a baseline phase, two elevated IOP phases (+10 and +20...

journal_title：Investigative ophthalmology & visual science

authors： Kiyota N,Shiga Y,Ichinohasama K,Yasuda M,Aizawa N,Omodaka K,Honda N,Kunikata H,Nakazawa T

更新日期：2018-07-02 00:00:00

abstract：Purpose:To investigate the effect of short-wavelength light (SL) on guinea pigs with lens-induced myopia (LIM) and the possible retinoic acid (RA)-related mechanisms. Methods:Two-week-old guinea pigs (n = 60) with monocular -5D lenses were reared under white light (WL, 580 lux) or SL (440 nm, 500 lux). The left eyes w...

journal_title：Investigative ophthalmology & visual science

authors： Yu M,Liu W,Wang B,Dai J

更新日期：2021-01-04 00:00:00

abstract：PURPOSE:To investigate the effect of TNF-alpha on leukocyte adhesion, vascular leakage, and apoptotic cell death in endotoxin-induced uveitis (EIU) in the rat. METHODS:EIU was induced in Long-Evans rats by a single footpad injection of lipopolysaccharide (LPS; 350 microg/kg) from Salmonella typhimurium. A single injec...

journal_title：Investigative ophthalmology & visual science

authors： Koizumi K,Poulaki V,Doehmen S,Welsandt G,Radetzky S,Lappas A,Kociok N,Kirchhof B,Joussen AM

更新日期：2003-05-01 00:00:00

abstract：PURPOSE:Overloading of unfolded or misfolded proteins in the endoplasmic reticulum (ER) can cause ER stress and activate the unfolded protein response (UPR) in the cell. The authors tested whether transgene overexpression in the mouse lens would activate the UPR. METHODS:Transgenic mice expressing proteins that either...

journal_title：Investigative ophthalmology & visual science

authors： Reneker LW,Chen H,Overbeek PA

更新日期：2011-04-04 00:00:00

abstract：PURPOSE:We characterized fluorescent bone marrow cells (YFP(+) BMCs) in the thy1-YFP mouse and determine if they promote trigeminal ganglion (TG) cell neurite growth. METHODS:Excimer laser annular keratectomy was performed in thy1-YFP mice, and corneas were imaged. BMCs were harvested from femur and tibia, and the exp...

journal_title：Investigative ophthalmology & visual science

authors： Sarkar J,Chaudhary S,Jassim SH,Ozturk O,Chamon W,Ganesh B,Tibrewal S,Gandhi S,Byun YS,Hallak J,Mahmud DL,Mahmud N,Rondelli D,Jain S

更新日期：2013-09-03 00:00:00

abstract：PURPOSE:The influences of diurnal rhythms involving a variety of ocular parameters are implicated in the development of myopia. The purpose of this study was to determine the expression of the melatonin receptor subtype proteins in chick ocular tissues and to examine the role of the circadian signaling molecule melaton...

journal_title：Investigative ophthalmology & visual science

authors： Rada JA,Wiechmann AF

更新日期：2006-01-01 00:00:00

abstract：PURPOSE:Contact inhibition has been implicated as an important antiproliferative mechanism in developing and mature corneal endothelium. Although exogenous TGF-beta2 and TGF-beta2 in aqueous humor suppress S-phase entry in cultured rat corneal endothelial cells, it is not known whether TGF-beta2 contributes to the mito...

journal_title：Investigative ophthalmology & visual science

authors： Joyce NC,Harris DL,Mello DM

更新日期：2002-07-01 00:00:00

abstract：PURPOSE:To investigate the effect of amino acids on ornithine cytotoxicity in ornithine-delta-aminotransferase (OAT)-deficient human retinal pigment epithelial (RPE) cells as an in vitro model of gyrate atrophy (GA) of the choroid and retina. METHODS:RPE cells were treated with 0.5 mM 5-fluoromethylornithine (5-FMOrn)...

journal_title：Investigative ophthalmology & visual science

authors： Nakauchi T,Ando A,Ueda-Yamada M,Yamazaki Y,Uyama M,Matsumura M,Ito S

更新日期：2003-11-01 00:00:00

abstract：:Secretory component (SC) was studied by indirect immunofluorescence of the ocular surface epithelium. We find that conjunctival epithelium produces this component, and that it is absent in the corneal epithelium. Conjunctival epithelium loses its SC staining within 1 to 2 days as it grows over a denuded corneal stroma...

journal_title：Investigative ophthalmology & visual science

authors： Liu SH,Tagawa Y,Prendergast RA,Franklin RM,Silverstein AM

更新日期：1981-01-01 00:00:00

abstract：PURPOSE:Cultured human corneal endothelial cells (cHCECs) are anticipated to become an alternative to donor corneas for the treatment of corneal endothelial dysfunction. However, cHCECs reportedly tend to exhibit chromosomal abnormality during in vitro cell division, thereby hampering their use in the clinical setting....

journal_title：Investigative ophthalmology & visual science

authors： Hamuro J,Ueno M,Toda M,Sotozono C,Montoya M,Kinoshita S

更新日期：2016-08-01 00:00:00

abstract：PURPOSE:Platelet-derived growth factor (PDGF) is an important stimulatory factor for proliferative retinopathies. Expression of PDGF-B in the retinas of transgenic mice (hemizygous rho/PDGF-B mice) results in rapid-onset retinal detachment caused by proliferation of glial cells, endothelial cells, and pericytes, wherea...

journal_title：Investigative ophthalmology & visual science

authors： Saishin Y,Saishin Y,Takahashi K,Seo MS,Melia M,Campochiaro PA

更新日期：2003-08-01 00:00:00

abstract：PURPOSE:In situ 2-photon excitation fluorescence (TPEF) of the human trabecular meshwork (TM) reveals beams of heterogeneous autofluorescence (AF) comprising high intensity fluorescent fibers (AF-high) on a background of lower intensity fluorescence (AF-low). To determine the sources of this AF heterogeneity, we imaged...

journal_title：Investigative ophthalmology & visual science

authors： Huang AS,Gonzalez JM Jr,Le PV,Heur M,Tan JC

更新日期：2013-07-18 00:00:00

abstract：PURPOSE:Angiogenesis, the formation of new capillary blood vessels, is an essential biological process under physiological conditions, including embryonic development, reproduction, and wound repair. Under pathologic conditions, this process plays a critical role in a variety of diseases such as cancer, rheumatoid arth...

journal_title：Investigative ophthalmology & visual science

authors： Pakneshan P,Birsner AE,Adini I,Becker CM,D'Amato RJ

更新日期：2008-09-01 00:00:00

abstract：PURPOSE:Histone lysine methylation (HKM) is an important epigenetic mechanism that establishes cell-specific gene expression and functions in development. However, epigenetic control of retinal development is poorly understood. To study the roles of HKM in retinogenesis, the authors examined the dynamic changes of thre...

journal_title：Investigative ophthalmology & visual science

authors： Rao RC,Tchedre KT,Malik MT,Coleman N,Fang Y,Marquez VE,Chen DF

更新日期：2010-12-01 00:00:00