当前位置： SCI文献检索 > JOURNAL OF DENTISTRY期刊下所有文献 > Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity.

Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity.

Abstract：

OBJECTIVES:A low pH environment is created by cariogenic bacteria. This study was aimed to measure pH of carious lesions intraorally using a micro-pH sensor, and assess predominant acid-producing cariogens by qPCR to differentiate caries activities. METHODS:103 dentine lesions classified as active or arrested caries based on the clinical and radiological examinations were collected from patients after intraoral measurement of the lesion surface pH using a micro-pH sensor. Quantitative detection of genomic DNA copies of target cariogenic bacteria (mutans streptococci and Lactobacillus spp.) in each lesion was performed using real-time PCR. Correlation between the pH ranges and the number of bacterial species was examined by Spearman test. RESULTS:50 samples were diagnosed as active and 53 as arrested lesions. Statistically significant difference was observed on average surface pH value between active and arrested lesions (p<0.05). Prevalence of Lactobacillus spp. was higher in active lesions than in arrested lesions (76% vs. 58% of samples, respectively). When the carious lesions were categorised into four different pH ranges (up to 5.5, from 5.6 to 5.8, from 5.9 to 6.1 and 6.2 or above), increased prevalence of Lactobacillus spp. was observed with decrease of pH levels. A significant negative relationship was found between pH value and number of Lactobacillus spp. (r=-0.209, p<0.05) but no such correlation was found for mutans streptococci. CONCLUSIONS:Intraoral pH measurement might be clinically useful to determine acidity of the local environment of carious lesions as one aspect of the caries activity assessment. CLINICAL SIGNIFICANCE:The population of certain bacteria may indicate activity of carious lesions. Intraoral pH measurement of the carious lesions using a micro-pH sensor may be a clinically feasible method for assessment of lesion acidity.

journal_name

J Dent

journal_title

Journal of dentistry

authors

Kuribayashi M,Kitasako Y,Matin K,Sadr A,Shida K,Tagami J

doi

10.1016/j.jdent.2011.12.013

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

222-8

issue

3

eissn

0300-5712

issn

1879-176X

pii

S0300-5712(11)00321-6

journal_volume

40

pub_type

杂志文章

相关文献

JOURNAL OF DENTISTRY文献大全
  • The potential for sodium hexametaphosphate (SHMP) found in common children drinks to limit acid production in the oral biofilm.

    abstract：OBJECTIVE:Sodium hexametaphosphate (SHMP) is a widely used industrial preservative commonly found in children's drinks. In this paper we examined the effect of SHMP incorporated into children's drinks on acid production by the oral biofilm by monitoring salivary concentrations of lactic acid. MATERIALS AND METHOD:Twel...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2006.08.003

    authors： Murphy J,Beighton D,Clark D,Bartlett D

    更新日期：2007-03-01 00:00:00

  • Cross-infection hazards associated with the use of pumice in dental laboratories.

    abstract：:The bacteriological contamination of pumice slurry in polishing units in a dental clinical area (high risk), a production laboratory (medium risk), and a non-clinical teaching laboratory (low risk), was investigated. Slurry samples taken from all three areas were found to be heavily contaminated with pathogenic organi...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/0300-5712(90)90030-i

    authors： Witt S,Hart P

    更新日期：1990-10-01 00:00:00

  • Mapping intraoral photographs on virtual teeth model.

    abstract：OBJECTIVES:Tooth shade is crucial to patient satisfaction in aesthetic dentistry. Two-dimensional (2D) clinical photographs have been widely used to record patients' tooth shade for creation of beautiful smile. However, in the digital workflow, 3D virtual teeth models in open file format contain only mesh information w...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2018.09.009

    authors： Lam WYH,Hsung RTC,Cheng LYY,Pow EHN

    更新日期：2018-12-01 00:00:00

  • Wish-fulfilling medicine and wish-fulfilling dentistry.

    abstract：OBJECTIVES:to explain the practice of wish-fulfilling medicine and how it relates to dentistry. SOURCES:Relevant papers, and reports from authoritative institutions were identified in Pubmed and Google Scholar. RESULTS:Wish-fulfilling medicine refers to services provided by professionals using medical methods in a me...

    journal_title：Journal of dentistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.jdent.2020.103302

    authors： Witter DJ,Kole JJJ,Brands WG,MacEntee MI,Creugers NHJ

    更新日期：2020-05-01 00:00:00

  • Casein phosphopeptide-amorphous calcium phosphate fluoride treatment enriches the symbiotic dental plaque microbiome in children.

    abstract：OBJECTIVES:The dysbiotic oral microbiome plays a key role in the pathogenesis of caries in children. Topical application of casein phosphopeptide-amorphous calcium phosphate containing fluoride (CPP-ACP/F) is an effective treatment modality for children with caries (CC). Hitherto the mechanism by which CPP-ACP/F module...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2021.103582

    authors： Widyarman AS,Udawatte NS,Theodorea CF,Apriani A,Richi M,Astoeti TE,Seneviratne CJ

    更新日期：2021-01-12 00:00:00

  • The optimisation of the initial viscosity of an encapsulated glass-ionomer restorative following different mechanical mixing regimes.

    abstract：OBJECTIVES:An optimisation of the mixing ratio of an encapsulated glass-ionomer restorative in terms of compressive strength, working characteristics and pore distribution following mechanical mixing with different mixing machines was undertaken. METHODS:Mean compressive fracture strengths, standard deviations and ass...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2005.05.008

    authors： Fleming GJ,Kenny SM,Barralet JE

    更新日期：2006-02-01 00:00:00

  • The clinical performance of adhesives.

    abstract：OBJECTIVES:Traditional mechanical methods of retaining restorative materials have been replaced to a large extent by tooth conserving adhesive restorative techniques. Because adhesives have been evolving so rapidly for the last few years, the timing is right for evaluating the clinical status of present day adhesives. ...

    journal_title：Journal of dentistry

    pub_type： 杂志文章,评审

    doi：10.1016/s0300-5712(96)00070-x

    authors： Van Meerbeek B,Perdigão J,Lambrechts P,Vanherle G

    更新日期：1998-01-01 00:00:00

  • Rapid monitoring of fluoride (F-) levels in blood plasma.

    abstract：:Evidence exists that plasma F- concentrations of 1.0 part/10(6) are nephrotoxic and that much lower levels disturb enamel mineralization. Ingested F(-)-containing gels, tablets and toothpastes all raise plasma F- levels significantly, as does prolonged exposure to fluorine-containing anaesthetic gases. There is, there...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/0300-5712(90)90254-c

    authors： Tyler JE,Poole DF,Kong KL

    更新日期：1990-02-01 00:00:00

  • Relative susceptibility of deciduous and permanent dental hard tissues to erosion by a low pH fruit drink in vitro.

    abstract：OBJECTIVES:The objectives of this study were two-fold: (1) to determine (by surfometry) loss of deciduous and permanent enamel and dentine following 15days' exposure to a single low pH orange drink; and (2) to determine (by surfometry) loss of deciduous and permanent enamel and dentine following exposure to the product...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/s0300-5712(99)00074-3

    authors： Hunter ML,West NX,Hughes JA,Newcombe RG,Addy M

    更新日期：2000-05-01 00:00:00

  • Accuracy of dental surveyors.

    abstract：:Maximum lateral forces as measured by strain gauges mounted on the analysing rod of a Nesor surveyor for four operators during surveying procedures ranged from 81 to 110 cN (mean 92.4 cN). Maximum lateral forces used when trimming blockout and inlay wax ranged from 159 to 184 cN (mean 169 cN) and 137 to 196 cN (mean 1...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/0300-5712(91)90012-n

    authors： Ahmad I,Waters NE

    更新日期：1991-06-01 00:00:00

  • Optical properties of current ceramics systems for laminate veneers.

    abstract：OBJECTIVES:Full-ceramic systems can be produced by different techniques (layering, heatpressing, CAD/CAM) and have various compositions with different crystalline contents that may affect the optical properties of laminate restorations. METHODS:A total of 60 specimens were prepared from e.max Press, e.max CAD, Empress...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2012.11.013

    authors： Bagis B,Turgut S

    更新日期：2013-08-01 00:00:00

  • Current uses of chlorhexidine for management of oral disease: a narrative review.

    abstract：OBJECTIVES:Chlorhexidine (CHX) is a commonly used antiseptic mouthwash, used by dental practitioners and the public, due to its antimicrobial effects. The aim of this article was to provide a narrative review of current antimicrobial uses of CHX relevant to dentistry in the context of oral diseases, highlighting need f...

    journal_title：Journal of dentistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.jdent.2020.103497

    authors： Brookes ZLS,Bescos R,Belfield LA,Ali K,Roberts A

    更新日期：2020-12-01 00:00:00

  • The interrelationship between xerogenic medication use, subjective oral dryness and tooth wear.

    abstract：OBJECTIVE:To investigate the association between xerogenic medication use and tooth wear among American adults and whether this association can be explained by subjective oral dryness. METHODS:We used data from 3578 adults who participated in the National Health and Nutritional Examination Survey (NHANES). Tooth wear ...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2020.103542

    authors： Alwaheidi HAA,O'Toole S,Bernabé E

    更新日期：2020-12-01 00:00:00

  • Quantifying and qualifying surface changes on dental hard tissues in vitro.

    abstract：INTRODUCTION:Tooth surface loss (TSL) is an increasingly recognised problem. We are therefore seeing an increase in the number and complexity of in vitro studies investigating factors that modify effects at the tooth surface. AIM:The aim of this paper is to provide a narrative review that illustrates the more commonly...

    journal_title：Journal of dentistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.jdent.2010.01.002

    authors： Field J,Waterhouse P,German M

    更新日期：2010-03-01 00:00:00

  • Water absorption of (RTV) silicone denture soft lining material.

    abstract：OBJECTIVES:The reason for high water absorption by room temperature vulcanizing (RTV) silicone denture soft lining materials has not been demonstrated previously. An experimental room-temperature vulcanizing (RTV) silicone soft lining material which had been shown to have good mechanical properties had high water absor...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/0300-5712(95)00032-1

    authors： Waters MG,Jagger RG,Winter RW

    更新日期：1996-01-01 00:00:00

  • Bioadhesion and retention of non-aqueous delivery systems in a dental hard tissue model.

    abstract：OBJECTIVES:Effective delivery of active agents to dental hard tissues is critical to product performance and pertinent to biofilm control, caries, erosion, hypersensitivity and tooth bleaching. The aim of this study was to investigate in vitro the bioadhesion and retention behaviour of non-aqueous delivery systems (NAD...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2010.06.005

    authors： Zaman MA,Martin GP,Rees GD

    更新日期：2010-09-01 00:00:00

  • Implant supported overdentures--the King's experience.

    abstract：OBJECTIVES:Astra Tech implants have been used to provide implant supported overdentures for 25 people, 13 with paired stud retention, 10 with paired magnetic retention and two with magnetic retention on three fixtures. RESULTS:The first case was completed in June 1990 and the last in May 1992. Of the 52 fixtures which...

    journal_title：Journal of dentistry

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0300-5712(97)87698-1

    authors： Davis DM

    更新日期：1997-01-01 00:00:00

  • The effect of first and second premolar extractions on third molars: A retrospective longitudinal study.

    abstract：OBJECTIVES:To analyse the effect of first and second premolar extractions on eruption space for upper and lower third molars and on third molar position and angulation during orthodontic treatment. METHODS:The sample consisted of 296 patients of which 218 patients were orthodontically treated without extraction and 78...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2017.03.007

    authors： Miclotte A,Grommen B,Cadenas de Llano-Pérula M,Verdonck A,Jacobs R,Willems G

    更新日期：2017-06-01 00:00:00

  • Association between microtensile bond strength and leakage in the indirect resin composite/dentin adhesively bonded joint.

    abstract：PURPOSE:Develop a methodology to correlate microtensile bond strength (microTBS) and leakage within the same site of a specimen and evaluate the predominate debond and leakage pathways. MATERIALS AND METHODS:A Class II (MO) slot cavity was prepared in 37 extracted human molars and restored with indirect resin composit...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/s0300-5712(00)00065-8

    authors： Guzmán-Ruiz S,Armstrong SR,Cobb DS,Vargas MA

    更新日期：2001-02-01 00:00:00

  • Curing behaviour of high-viscosity bulk-fill composites.

    abstract：OBJECTIVES:This study aimed to assess the effect of curing conditions--exposure time, mode, energy density, and exposure distance--on the mechanical properties of high-viscosity bulk-fill resin-based composites (RBCs) measured at simulated clinical relevant filling depths. METHODS:Three high-viscosity bulk-fill RBCs w...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2014.05.012

    authors： Ilie N,Stark K

    更新日期：2014-08-01 00:00:00

  • Up to 17-year controlled clinical study on post-and-cores and covering crowns.

    abstract：OBJECTIVE:The aim of this long-term follow-up was to collect up to 17-year survival data of different metal post-and-core restorations with a covering crown. METHODS:At initiation of the study, a controlled clinical trial, single tooth was provided with an artificial covering crown, by 18 operators. Restorations under...

    journal_title：Journal of dentistry

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.jdent.2007.07.006

    authors： Fokkinga WA,Kreulen CM,Bronkhorst EM,Creugers NH

    更新日期：2007-10-01 00:00:00

  • Three-dimensional analysis of pulp chambers in maxillary second deciduous molars.

    abstract：OBJECTIVE:The purpose of this study was to clarify the positional relationship between the crown contour and the pulp chamber using micro-CT system for restorations of maxillary second deciduous molar. METHODS:Five maxillary second deciduous molar teeth each from the deciduous dentition and the mixed dentition periods...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2005.12.001

    authors： Amano M,Agematsu H,Abe S,Usami A,Matsunaga S,Suto K,Ide Y

    更新日期：2006-08-01 00:00:00

  • Inhibition of hybrid layer degradation by cavity pretreatment: Meta- and trial sequential analysis.

    abstract：OBJECTIVES:Inhibition of hybrid layer degradation, for example via inhibition of matrix-metallo-proteinases (MMP) could reduce risk of retention loss and failure of adhesively placed restorations. This systematic review investigated such inhibitory pretreatment qualitatively and via meta- and trial-sequential-analysis....

    journal_title：Journal of dentistry

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.jdent.2016.04.007

    authors： Göstemeyer G,Schwendicke F

    更新日期：2016-06-01 00:00:00

  • Laboratory performance and fracture resistance of CAD/CAM implant-supported tooth-coloured anterior FDPs.

    abstract：OBJECTIVES:This study investigated the in-vitro performance and fracture force of anterior implant-supported tooth-coloured fixed dental prosthesis (FDPs). Different material types with varying flexural strength and modulus of elasticity were compared with screw-retained or bonded application. MATERIALS AND METHODS:Id...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2020.103326

    authors： Zacher J,Bauer R,Strasser T,Rosentritt M

    更新日期：2020-05-01 00:00:00

  • Effect of shading technique and thickness on color stability and translucency of new generation translucent zirconia.

    abstract：OBJECTIVE:To evaluate the effect of shading technique and thickness on the color stability and translucency of translucent zirconia after coffee thermocycling. METHODS:Specimens in different thicknesses (1; 1.5; 2 mm) (n = 4 for each thickness) were sectioned from translucent preshaded zirconia (Pre) and externally sh...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2018.03.011

    authors： Alp G,Subaşı MG,Seghi RR,Johnston WM,Yilmaz B

    更新日期：2018-06-01 00:00:00

  • The effect of unstimulated and stimulated whole saliva on extrinsic staining in vitro--a developmental method.

    abstract：OBJECTIVES:To detect any differences in the propensity of unstimulated and stimulated, individual whole saliva to cause in vitro staining by chlorhexidine and tea. METHODS:Unstimulated and stimulated human saliva was collected on a daily basis and used to coat optically clear acrylic specimens. Specimens were subjecte...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/s0300-5712(02)00053-2

    authors： Sheen S,Banfield N,Addy M

    更新日期：2002-09-01 00:00:00

  • A practice-based study of the sealant treatment effectiveness in Finns.

    abstract：OBJECTIVES:The effectiveness of fissure sealing in preventing dental caries in a subject-specific fashion was studied in order to forge a link between the controlled trials and knowledge creation in clinical practice. METHODS:The subjects were divided retrospectively into three categories according to the sealant trea...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2006.10.009

    authors： Leskinen K,Salo S,Suni J,Larmas M

    更新日期：2007-04-01 00:00:00

  • Long-term follow-up of composite resin restorations with self-etching adhesives.

    abstract：OBJECTIVE:To test the hypothesis, derived from a previous short-term (7-day) assessment, that the absence of conventional pulp protection is not responsible for long-term pulp complications of composite resin restorations with self-etching adhesives. METHODS:All 150 patients who received the restorations with self-etc...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2007.02.005

    authors： Unemori M,Matsuya Y,Hyakutake H,Matsuya S,Goto Y,Akamine A

    更新日期：2007-06-01 00:00:00

  • The effects of mechanical and hydrothermal aging on microstructure and biaxial flexural strength of an anterior and a posterior monolithic zirconia.

    abstract：OBJECTIVES:To evaluate the effect of hydrothermal aging (H), mechanical cycling (M), and the combination of hydrothermal plus mechanical cycling (H+M) on biaxial flexural strength (BFS) and microstructure of two monolithic zirconias, indicated for anterior (AMZ) or posterior restorations (PMZ) and a conventional zircon...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2017.05.021

    authors： Muñoz EM,Longhini D,Antonio SG,Adabo GL

    更新日期：2017-08-01 00:00:00

  • Human gingival fibroblasts function is stimulated on machined hydrided titanium zirconium dental implants.

    abstract：OBJECTIVES:The aim of this study was to evaluate the influence of different titanium zirconium (TiZr) alloy surfaces on primary human gingival fibroblasts (HGF) for improved soft tissue integration of dental implants. METHODS:TiZr polished, machined and machined+HCl/H2SO4 acid-etched surfaces were modified by cathodic...

    journal_title：Journal of dentistry

    pub_type： 杂志文章

    doi：10.1016/j.jdent.2013.11.003

    authors： Gómez-Florit M,Xing R,Ramis JM,Taxt-Lamolle S,Haugen HJ,Lyngstadaas SP,Monjo M

    更新日期：2014-01-01 00:00:00