Abstract:
PURPOSE:To determine the impact of substratum compliance and latrunculin-B (Lat-B), both alone and together, on fundamental human trabecular meshwork (HTM) cell behavior. Lat-B is a reversible actin cytoskeleton disruptor that decreases resistance to aqueous humor outflow and decreases intraocular pressure. METHODS:HTM cells were cultured on polyacrylamide hydrogels possessing values for compliance that mimic those reported for normal and glaucomatous HTM, or tissue culture plastic (TCP). Cells were treated with 0.2 μM or 2.0 μM Lat-B in dimethyl sulfoxide (DMSO) or DMSO alone. The impact of substratum compliance and/or Lat-B treatment on cell attachment, proliferation, surface area, aspect ratio, and migration were investigated. RESULTS:HTM cells had profoundly decreased attachment and proliferation rates when cultured on hydrogels possessing compliance values that mimic those found for healthy HTM. The effect of Lat-B treatment on HTM cell surface area was less for cells cultured on more compliant hydrogels compared with TCP. HTM cell migration was increased on stiffer hydrogels that mimic the compliance of glaucomatous HTM and on TCP in comparison with more compliant hydrogels. Lat-B treatment decreased cellular migration on all surfaces for at least 7 hours after treatment. CONCLUSIONS:Substratum compliance profoundly influenced HTM cell behaviors and modulated the response of HTM cells to Lat-B. The inclusion of substratum compliance that reflects healthy or glaucomatous HTM results in cell behaviors and responses to therapeutic agents in vitro that may more accurately reflect in vivo conditions.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Wood JA,McKee CT,Thomasy SM,Fischer ME,Shah NM,Murphy CJ,Russell Pdoi
10.1167/iovs.11-7857subject
Has Abstractpub_date
2011-12-02 00:00:00pages
9298-303issue
13eissn
0146-0404issn
1552-5783pii
iovs.11-7857journal_volume
52pub_type
杂志文章abstract:PURPOSE:The incidence of blindness is increasing, in part, because of abnormal ocular neovascularization. Anti-VEGF therapies have yielded impressive results; however, they are not a cure for blindness. Recently, metallothioneins (MTs) 1 and 2 have been implicated in the process of angiogenesis. Therefore, we investiga...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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abstract::Freshly isolated peripheral blood lymphocytes from patients (PBL-P) with recurrent herpetic corneal and skin lesions, and controls (PBL-C) with no recollection of herpetic disease, effectively lysed herpes simplex virus type 1 (HSV-1) infected but not uninfected allogeneic fibroblasts. However, after 48 hr of stimulat...
journal_title:Investigative ophthalmology & visual science
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abstract:PURPOSE:To examine the expression of c-fos proto-oncogene and phosphorylation of cAMP responsive element binding (CREB) protein in the rat retina after changes in the light-dark condition. METHODS:Rats were exposed to both steady light and flashing light and were killed at the end of light exposure. The retinas were a...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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更新日期:2012-06-20 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1992-12-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:2001-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1995-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1998-03-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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更新日期:2007-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2007-07-01 00:00:00
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更新日期:2015-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:PURPOSE:To create a binocular statistical eye model based on previously measured ocular biometric data. METHODS:Thirty-nine parameters were determined for a group of 127 healthy subjects (37 male, 90 female; 96.8% Caucasian) with an average age of 39.9 ± 12.2 years and spherical equivalent refraction of -0.98 ± 1.77 D...
journal_title:Investigative ophthalmology & visual science
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更新日期:2011-06-23 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2006-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2004-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2019-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:1985-04-01 00:00:00