Abstract:
:Retinal ultrastructure was examined at various intervals following a single intravitreal injection of 100-4,000 micrograms of gentamicin in rabbit eyes. Three days after injections of 100-500 micrograms, numerous abnormal lamellar lysosomal inclusions were observed in the retinal pigment epithelium (RPE) and in macrophages in the subretinal space. These changes were typical of drug-induced lipid storage and were comparable to inclusions reported in kidney and other tissues as manifestations of gentamicin toxicity. One week after similar injections, focal areas of RPE necrosis and hyperplasia with disruption of outer segments appeared, but the inner segments and inner retina were intact. Doses of 800-4,000 micrograms produced a combined picture of RPE/macrophage lipidosis within the first 3 days, with increasing, superimposed, inner, retinal necrosis. This study provides the first evidence of lysosomal alterations in ocular tissues following the intravitreal injection of gentamicin and implicates the RPE as the primary site of observed toxicity.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
D'Amico DJ,Libert J,Kenyon KR,Hanninen LA,Caspers-Velu Lsubject
Has Abstractpub_date
1984-05-01 00:00:00pages
564-72issue
5eissn
0146-0404issn
1552-5783journal_volume
25pub_type
杂志文章abstract:PURPOSE:Antiangiogenic treatment is a promising new therapy for angiogenesis-dependent diseases. In the current study, the biologic effects on pathologic and physiological angiogenesis in the retina of angiostatin, a very potent angiogenesis inhibitor were determined. In addition, the effects of angiostatin on the grow...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-12-01 00:00:00
abstract::Evidence for a population of acetylcholine (ACh) receptors in the human retina is presented. The authors have used the irreversible ligand 3H-propylbenzilylcholine mustard (3H-PrBCM) to label muscarinic receptors. 3H- or 125I-alpha-bungarotoxin (alpha-BTx) was used to label putative nicotinic receptors. Muscarinic rec...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract:Purpose:Optical coherence tomography's (OCT) third outer retinal band has been attributed to the zone of interdigitation between RPE cells and cone outer segments. The purpose of this paper is to investigate the structure of this band with adaptive optics (AO)-OCT. Methods:Using AO-OCT, images were obtained from two s...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-21138
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:In MRL/Mp-lpr/lpr (MRL/lpr) and MRL/Mp-+/+ (MRL/+) mice, a T-cell-driven lacrimal gland inflammation spontaneously develops that is a model for Sjögren's syndrome. The lacrimal gland lesions in these mice were evaluated by immunohistochemistry for the relative contributions of T-helper (Th)1 versus Th2 immune r...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract:Purpose:The purpose of this study was to measure change in anterior lamina cribrosa depth (ALD) globally and regionally in glaucoma eyes at different intraocular pressures (IOP). Methods:Twenty-seven glaucoma patients were imaged before and after IOP-lowering procedures using optical coherence tomography. The anterior...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-21321
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:To test whether a diet supplemented with coenzyme Q10 (CoQ10) ameliorates glutamate excitotoxicity and oxidative stress-mediated retinal ganglion cell (RGC) degeneration by preventing mitochondrial alterations in the retina of glaucomatous DBA/2J mice. METHODS:Preglaucomatous DBA/2J and age-matched control DBA...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-12564
更新日期:2014-02-18 00:00:00
abstract:PURPOSE:To evaluate the visual and refractive outcome for four wavefront-guided surface ablation (WGSA) techniques (LASEK, LASEK flap-off [LASEK FO], Epi-LASIK, and Epi-LASIK flap-off [Epi-LASIK FO]) in a large myopic population. METHODS:This retrospective review included 1000 myopic eyes (spherical equivalent [SE] -1...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-10387
更新日期:2013-01-21 00:00:00
abstract::Gamma-interferon (G-IFN) exhibits antiproliferative effects, inhibits collagen synthesis by fibroblasts, and may help to regulate the turnover of fibrous tissue. Fibroblasts from Tenon's capsule are cellular components that contribute to unsuccessful glaucoma filtration surgery. Therefore, the authors studied the effe...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract:PURPOSE:To describe the normal physiological relationship between retinal arteriolar and venular caliber and retinal nerve fiber layer (RNFL), macular, and optic nerve head parameters. METHODS:The Sydney Childhood Eye Study assessed 2038 adolescents aged 12 years. Retinal vessel calibers were measured from digital fun...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
doi:10.1167/iovs.09-3878
更新日期:2009-12-01 00:00:00
abstract:Purpose:To evaluate the contribution of fixational saccades toward fixation instability in strabismic monkeys. Methods:Binocular eye movements were measured as six experimental monkeys (five strabismic monkeys and one monkey with downbeat nystagmus) and one normal monkey fixated targets of two shapes (Optotype, Disk) ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.17-22389
更新日期:2017-11-01 00:00:00
abstract:PURPOSE:To compare the diagnostic value of Bruch's membrane opening minimum rim width (BMO-MRW) and retinal nerve fiber layer thickness (RNFLT) in patients with ocular hypertension, preperimetric, and perimetric glaucoma. METHODS:One hundred eighty-one eyes consisting of 40 healthy controls, 41 ocular hypertensive sub...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,随机对照试验
doi:10.1167/iovs.15-18906
更新日期:2016-07-01 00:00:00
abstract:PURPOSE:Regulatory CD4+ T cells (T regs) arise in the spleens of mice with anterior chamber-associated immune deviation (ACAID), an eye-derived tolerance evoked by injection of antigen into the ocular anterior chamber (AC). The current study was conducted to investigate the possibility that these T regs express CD25 an...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.05-0110
更新日期:2006-03-01 00:00:00
abstract:Purpose:We previously reported that a specific treadmill running exercise regimen protects against light-induced retinal degeneration (LIRD) in mice. We hypothesized that this protective effect varies with running intensity. To test this, mice undergoing LIRD were run at different treadmill speeds and retinal function ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-25883
更新日期:2019-04-01 00:00:00
abstract:Purpose:To evaluate the corneal endothelial characteristics across the primary angle closure (PAC) disease spectrum amongst patients diagnosed as PAC suspects (PACS), PAC, PAC glaucoma (PACG), and previous acute PAC (APAC). Methods:We analyzed a total of 529 subjects (51 PACS, 170 PAC, 234 PACG, and 74 with previous A...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-24939
更新日期:2018-09-04 00:00:00
abstract::Exposure of adult male and female albino rats to high-intensity illumination for 17.5 hr results in a marked, transient exophthalmos, which persists for approximately 48 hr after the light exposure. Upon examination of the Harderian glands in these rats, a significant glandular weight increase was observed up to 2 day...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1979-12-01 00:00:00
abstract:PURPOSE:Multidrug resistance protein 4 (MRP4) effluxes a wide variety of endogenous compounds, including cyclic adenosine monophosphate (cAMP), and is exclusively expressed in vascular endothelial cells (ECs) of the retina. This study aimed to investigate the role of MRP4 in retinal vascular development. METHODS:The r...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.12-10781
更新日期:2012-12-07 00:00:00
abstract:Purpose:To develop deep learning (DL) models for the automatic detection of optical coherence tomography (OCT) measures of diabetic macular thickening (MT) from color fundus photographs (CFPs). Methods:Retrospective analysis on 17,997 CFPs and their associated OCT measurements from the phase 3 RIDE/RISE diabetic macul...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-25634
更新日期:2019-03-01 00:00:00
abstract:Purpose:To characterize inheritance, penetrance, and trinucleotide repeat expansion stability in Fuchs endothelial corneal dystrophy (FECD). Methods:One thousand unrelated and related subjects with and without FECD were prospectively recruited. CTG18.1 repeat length (CTG18.1L) was determined via short tandem repeat as...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.62.1.17
更新日期:2021-01-04 00:00:00
abstract:PURPOSE:To evaluate age-related Meibomian gland (MG) changes by in vivo laser scanning confocal microscopy (LSCM). METHODS:Asymptomatic healthy subjects (n=100, age range 20-83 years) with an Ocular Surface Disease Index score of less than 13 were consecutively enrolled. Two additional groups, one composed of subjects...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-11914
更新日期:2013-07-16 00:00:00
abstract:PURPOSE:To test the hypothesis that longitudinal differences between retinal nerve fiber layer (RNFL) birefringence, measured by scanning laser polarimetry (SLP), and RNFL thickness, measured by optical coherence tomography (OCT), are informative about the state of axonal degeneration. METHODS:Colchicine was injected ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.07-0872
更新日期:2008-01-01 00:00:00
abstract:Purpose:To analyze the anti-inflammatory and antioxidative effects of Camellia japonica (CJ) on human corneal epithelial (HCE) cells and its therapeutic effects in a mouse model of experimental dry eye (EDE). Methods:Camellia japonica extracts of varying concentrations (0.001%, 0.01%, and 0.1%) were used to treat HCE ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-20634
更新日期:2017-02-01 00:00:00
abstract::Autoregulation of the retinal circulation in response to an acute elevation of intraocular pressure was investigated in 17 subjects (23 eyes) with no ocular abnormalities, by means of the blue field entoptic phenomenon. This phenomenon allows a person to observe leukocytes flowing in his own macular capillaries. Subje...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::Ocular sympathetic nerves were stimulated chronically in awake rabbits using electrodes unilaterally implanted on the cervical sympathetic trunk. IOP was measured by pneumatonometry and aqueous inflow was measured by fluorophotometry. In each animal, continuous trains of 1 msec pulses were delivered by means of a port...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::Three methods of corneal epithelial preparation for subsequent analysis of epithelial glycogen and DNA content were compared: scraping, lamellar stripping, and in situ freezing. All three methods gave good recovery of these substances. However, there was incomplete recovery of the sample using the in situ freezing met...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract:Purpose:To define the nature and extent of temporal frequency abnormalities in diabetics who have mild or no nonproliferative diabetic retinopathy (NPDR) by using the flicker electroretinogram (ERG). Methods:Light-adapted flicker ERGs were recorded from 20 diabetics who have no clinically apparent retinopathy, 20 diab...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.18-25199
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:To investigate concentrations of growth factors and inflammatory cytokines in eyes with central (CRVO) and branch (BRVO) retinal vein occlusion before and during therapy with bevacizumab and to identify associations with disease activity. METHODS:In a prospective clinical trial, 13 eyes of patients with CRVO (...
journal_title:Investigative ophthalmology & visual science
pub_type: 临床试验,杂志文章
doi:10.1167/iovs.08-2510
更新日期:2009-03-01 00:00:00
abstract::Iron is a potent generator of oxidative damage whose levels increase with age, potentially exacerbating age-related diseases. Several lines of evidence suggest that iron accumulation may be a factor in age-related macular degeneration (AMD). AMD retinas have more iron within the photoreceptors, RPE, and drusen than do...
journal_title:Investigative ophthalmology & visual science
pub_type:
doi:10.1167/iovs.06-0568
更新日期:2006-11-01 00:00:00
abstract:PURPOSE:To study pathologic changes in the photoreceptors in eyes with idiopathic macular telangiectasia (MacTel) type 2 using adaptive optics scanning laser ophthalmoscopy (AO-SLO). METHODS:Thirteen eyes with nonproliferative MacTel type 2 and 10 normal eyes underwent a full ophthalmologic examination, spectral-domai...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-7251
更新日期:2011-07-25 00:00:00
abstract:PURPOSE:Children with idiopathic infantile nystagmus (IIN) exhibit visual acuity deficits that have been modeled in terms of foveation characteristics of the nystagmus waveform. Here we present evidence for an additional component of acuity loss associated with the deprivation experienced during the sensitive period of...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-11992
更新日期:2013-05-01 00:00:00
abstract:PURPOSE:To investigate corneal nerve damage and its subsequent regeneration after excimer laser photokeratectomy in rabbit eyes. METHODS:Corneal photokeratectomy was performed on the right eyes of 34 pigmented rabbits, while the left eyes were used as a control. A Summit UV 200 excimer laser (Summit Technology, Waltha...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00