Abstract:
:We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m(6)A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO.
journal_name
Nat Chem Bioljournal_title
Nature chemical biologyauthors
Jia G,Fu Y,Zhao X,Dai Q,Zheng G,Yang Y,Yi C,Lindahl T,Pan T,Yang YG,He Cdoi
10.1038/nchembio.687subject
Has Abstractpub_date
2011-10-16 00:00:00pages
885-7issue
12eissn
1552-4450issn
1552-4469pii
nchembio.687journal_volume
7pub_type
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