N6-methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO.

Abstract:

:We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m(6)A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO.

journal_name

Nat Chem Biol

journal_title

Nature chemical biology

authors

Jia G,Fu Y,Zhao X,Dai Q,Zheng G,Yang Y,Yi C,Lindahl T,Pan T,Yang YG,He C

doi

10.1038/nchembio.687

subject

Has Abstract

pub_date

2011-10-16 00:00:00

pages

885-7

issue

12

eissn

1552-4450

issn

1552-4469

pii

nchembio.687

journal_volume

7

pub_type

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