Estimation of the genetic diversity in tetraploid alfalfa populations based on RAPD markers for breeding purposes.


:Alfalfa is an autotetraploid, allogamous and heterozygous forage legume, whose varieties are synthetic populations. Due to the complex nature of the species, information about genetic diversity of germplasm used in any alfalfa breeding program is most beneficial. The genetic diversity of five alfalfa varieties, involved in progeny tests at Institute of Field and Vegetable Crops, was characterized based on RAPD markers. A total of 60 primers were screened, out of which 17 were selected for the analysis of genetic diversity. A total of 156 polymorphic bands were generated, with 10.6 bands per primer. Number and percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon's information index were used to estimate genetic variation. Variety Zuzana had the highest values for all tested parameters, exhibiting the highest level of variation, whereas variety RSI 20 exhibited the lowest. Analysis of molecular variance (AMOVA) showed that 88.39% of the total genetic variation was attributed to intra-varietal variance. The cluster analysis for individual samples and varieties revealed differences in their population structures: variety Zuzana showed a very high level of genetic variation, Banat and Ghareh were divided in subpopulations, while Pecy and RSI 20 were relatively uniform. Ways of exploiting the investigated germplasm in the breeding programs are suggested in this paper, depending on their population structure and diversity. The RAPD analysis shows potential to be applied in analysis of parental populations in semi-hybrid alfalfa breeding program in both, development of new homogenous germplasm, and identification of promising, complementary germplasm.


Int J Mol Sci


Nagl N,Taski-Ajdukovic K,Barac G,Baburski A,Seccareccia I,Milic D,Katic S




Has Abstract


2011-01-01 00:00:00












  • RNA interference in mammalia cells by RNA-3'-PNA chimeras.

    abstract::The discovery of siRNAs as the mediators of RNA interference has led to an increasing interest in their therapeutic applications. Chemical modifications are introduced into siRNAs to optimize the potency, the stability and the pharmacokinetic properties in vivo. Here, we synthesize and test the effects of RNA-3'-PNA c...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Potenza N,Moggio L,Milano G,Salvatore V,Di Blasio B,Russo A,Messere A

    更新日期:2008-03-01 00:00:00

  • Dual Effect of Soloxolone Methyl on LPS-Induced Inflammation In Vitro and In Vivo.

    abstract::Plant-extracted triterpenoids belong to a class of bioactive compounds with pleotropic functions, including antioxidant, anti-cancer, and anti-inflammatory effects. In this work, we investigated the anti-inflammatory and anti-oxidative activities of a semisynthetic derivative of 18βH-glycyrrhetinic acid (18βH-GA), sol...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Markov AV,Sen'kova AV,Babich VO,Odarenko KV,Talyshev VA,Salomatina OV,Salakhutdinov NF,Zenkova MA,Logashenko EB

    更新日期:2020-10-23 00:00:00

  • Actin and Actin-Associated Proteins in Extracellular Vesicles Shed by Osteoclasts.

    abstract::Extracellular vesicles (EVs) are shed by all eukaryotic cells and have emerged as important intercellular regulators. EVs released by osteoclasts were recently identified as important coupling factors in bone remodeling. They are shed as osteoclasts resorb bone and stimulate osteoblasts to form bone to replace the bon...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Holliday LS,Faria LP,Rody WJ Jr

    更新日期:2019-12-25 00:00:00

  • Effect of Different Phospholipids on α-Secretase Activity in the Non-Amyloidogenic Pathway of Alzheimer's Disease.

    abstract::Alzheimer's disease (AD) is characterized by extracellular accumulation of amyloid-β peptide (Aβ), generated by proteolytic processing of the amyloid precursor protein (APP) by β- and γ-secretase. Aβ generation is inhibited when the initial ectodomain shedding is caused by α-secretase, cleaving APP within the Aβ domai...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Grimm MO,Haupenthal VJ,Rothhaar TL,Zimmer VC,Grösgen S,Hundsdörfer B,Lehmann J,Grimm HS,Hartmann T

    更新日期:2013-03-13 00:00:00

  • Spliceosome-Associated microRNAs Signify Breast Cancer Cells and Portray Potential Novel Nuclear Targets.

    abstract::MicroRNAs (miRNAs) act as negative regulators of gene expression in the cytoplasm. Previous studies have identified the presence of miRNAs in the nucleus. Here we study human breast cancer-derived cell-lines (MCF-7 and MDA-MB-231) and a non-tumorigenic cell-line (MCF-10A) and compare their miRNA sequences at the splic...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mahlab-Aviv S,Zohar K,Cohen Y,Peretz AR,Eliyahu T,Linial M,Sperling R

    更新日期:2020-10-30 00:00:00

  • Molecular cloning and expression analysis of P-selectin from Zebrafish (Danio rerio).

    abstract::The glycoprotein P-selectin belongs to the selectin family of cell adhesion molecules. In this study, we cloned the full-length cDNA of P-selectin from zebrafish (Danio rerio) by the method of rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). Zebrafish P-selectin cDNA is 2,800 bp and encodes a put...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sun G,Pan J,Liu K,Wang X,Wang S

    更新日期:2010-11-17 00:00:00

  • A Multi-Scale Study of Water Dynamics under Confinement, Exploiting Numerical Simulations in Relation to NMR Relaxometry, PGSE and NMR Micro-Imaging Experiments: An Application to the Clay/Water Interface.

    abstract::Water mobility within the porous network of dense clay sediments was investigated over a broad dynamical range by using 2H nuclear magnetic resonance spectroscopy. Multi-quanta 2H NMR spectroscopy and relaxation measurements were first performed to identify the contributions of the various relaxation mechanisms monito...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Porion P,Delville A

    更新日期:2020-06-30 00:00:00

  • Preparation of Fish Skin Gelatin-Based Nanofibers Incorporating Cinnamaldehyde by Solution Blow Spinning.

    abstract::Cinnamaldehyde, a natural preservative that can non-specifically deactivate foodborne pathogens, was successfully incorporated into fish skin gelatin (FSG) solutions and blow spun into uniform nanofibers. The effects of cinnamaldehyde ratios (5-30%, w/w FSG) on physicochemical properties of fiber-forming emulsions (FF...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Liu F,Türker Saricaoglu F,Avena-Bustillos RJ,Bridges DF,Takeoka GR,Wu VCH,Chiou BS,Wood DF,McHugh TH,Zhong F

    更新日期:2018-02-22 00:00:00

  • Comparative Small RNA Analysis of Pollen Development in Autotetraploid and Diploid Rice.

    abstract::MicroRNAs (miRNAs) play key roles in plant reproduction. However, knowledge on microRNAome analysis in autotetraploid rice is rather limited. Here, high-throughput sequencing technology was employed to analyze miRNAomes during pollen development in diploid and polyploid rice. A total of 172 differentially expressed mi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Li X,Shahid MQ,Wu J,Wang L,Liu X,Lu Y

    更新日期:2016-04-12 00:00:00

  • Real-Time 3D Imaging and Inhibition Analysis of Various Amyloid Aggregations Using Quantum Dots.

    abstract::Amyloidosis refers to aggregates of protein that accumulate and are deposited as amyloid fibrils into plaques. When these are detected in organs, they are the main hallmark of Alzheimer's disease, Parkinson's disease, and other related diseases. Recent medical advances have shown that many precursors and proteins can ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Lin X,Galaqin N,Tainaka R,Shimamori K,Kuragano M,Noguchi TQP,Tokuraku K

    更新日期:2020-03-13 00:00:00

  • Different impacts of cardiovascular risk factors on oxidative stress.

    abstract::The objective of the study was to evaluate oxidative stress (OS) status in subjects with different cardiovascular risk factors. With this in mind, we have studied three models of high cardiovascular risk: hypertension (HT) with and without metabolic syndrome, familial hypercholesterolemia (FH) and familial combined hy...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Mansego ML,Redon J,Martinez-Hervas S,Real JT,Martinez F,Blesa S,Gonzalez-Albert V,Saez GT,Carmena R,Chaves FJ

    更新日期:2011-01-01 00:00:00

  • Natural products as a source for treating neglected parasitic diseases.

    abstract::Infectious diseases caused by parasites are a major threat for the entire mankind, especially in the tropics. More than 1 billion people world-wide are directly exposed to tropical parasites such as the causative agents of trypanosomiasis, leishmaniasis, schistosomiasis, lymphatic filariasis and onchocerciasis, which ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ndjonka D,Rapado LN,Silber AM,Liebau E,Wrenger C

    更新日期:2013-02-06 00:00:00

  • STVNa Attenuates Isoproterenol-Induced Cardiac Hypertrophy Response through the HDAC4 and Prdx2/ROS/Trx1 Pathways.

    abstract::Recent data show that cardiac hypertrophy contributes substantially to the overall heart failure burden. Mitochondrial dysfunction is a common feature of cardiac hypertrophy. Recent studies have reported that isosteviol inhibits myocardial ischemia-reperfusion injury in guinea pigs and H9c2 cells. This work investigat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Liu F,Su H,Liu B,Mei Y,Ke Q,Sun X,Tan W

    更新日期:2020-01-20 00:00:00

  • Comparative Proteomic Analysis of Lysine Acetylation in Fish CIK Cells Infected with Aquareovirus.

    abstract::Grass carp (Ctenopharyngodon idellus) is an important worldwide commercial freshwater culture species. However, grass carp reovirus (GCRV) causes serious hemorrhagic disease in fingerlings and yearlings of fishes. To understand the molecular pathogenesis of host cells during GCRV infection, intensive proteomic quantif...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Guo H,Zhang J,Wang Y,Bu C,Zhou Y,Fang Q

    更新日期:2017-11-14 00:00:00

  • Chlorogenic Acid and Its Microbial Metabolites Exert Anti-Proliferative Effects, S-Phase Cell-Cycle Arrest and Apoptosis in Human Colon Cancer Caco-2 Cells.

    abstract::Chlorogenic acid (CGA) decreases colon cancer-cell proliferation but the combined anti-cancer effects of CGA with its major colonic microbial metabolites, caffeic acid (CA), 3-phenylpropionic acid (3-PPA) and benzoic acid (BA), needs elucidation as they occur together in colonic digesta. Caco-2 cancer cells were treat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sadeghi Ekbatan S,Li XQ,Ghorbani M,Azadi B,Kubow S

    更新日期:2018-03-03 00:00:00

  • A crosslinking analysis of GAP-43 interactions with other proteins in differentiated N1E-115 cells.

    abstract::It has been suggested that GAP-43 (growth-associated protein) binds to various proteins in growing neurons as part of its mechanism of action. To test this hypothesis in vivo, differentiated N1E-115 neuroblastoma cells were labeled with [(35)S]-amino acids and were treated with a cleavable crosslinking reagent. The ce...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Ollom CM,Denny JB

    更新日期:2008-09-01 00:00:00

  • Targeting Cancer Stem Cells to Overcome Chemoresistance.

    abstract::Cancers are heterogeneous at the cell level, and the mechanisms leading to cancer heterogeneity could be clonal evolution or cancer stem cells. Cancer stem cells are resistant to most anti-cancer treatments and could be preferential targets to reverse this resistance, either targeting stemness pathways or cancer stem ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Nunes T,Hamdan D,Leboeuf C,El Bouchtaoui M,Gapihan G,Nguyen TT,Meles S,Angeli E,Ratajczak P,Lu H,Di Benedetto M,Bousquet G,Janin A

    更新日期:2018-12-13 00:00:00

  • Mixed-Generation PAMAM G3-G0 Megamer as a Drug Delivery System for Nimesulide: Antitumor Activity of the Conjugate Against Human Squamous Carcinoma and Glioblastoma Cells.

    abstract::Polyhydroxylated dendrimer was synthesized from poly(amidoamine) (PAMAM) dendrimer generation 3 by addition of glycidol (G3gl). G3gl megamer was further modified by binding PAMAM G0 dendrimers by activation of G3gl with p-nitrophenylchloroformate, followed by the addition of excess PAMAM G0 and purification using dial...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zaręba M,Sareło P,Kopaczyńska M,Białońska A,Uram Ł,Walczak M,Aebisher D,Wołowiec S

    更新日期:2019-10-09 00:00:00

  • Impact of Selected Signaling Proteins on SNAIL 1 and SNAIL 2 Expression in Ovarian Cancer Cell Lines in Relation to Cells' Cisplatin Resistance and EMT Markers Level.

    abstract::It has been increasingly recognized that SNAIL1 and SNAIL2, as major EMT-inducers, might also be involved in drug resistance of cancer cells. We sought to determine a relation between SNAIL1/2, E-cadherin and N-cadherin expression, as well as ovarian cancer cells' resistance to cisplatin and EMT markers' level. Thus, ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Kielbik M,Szulc-Kielbik I,Klink M

    更新日期:2021-01-19 00:00:00

  • Immune Complexes Impaired Glomerular Endothelial Cell Functions in Lupus Nephritis.

    abstract::Lupus nephritis (LN) is one of the most common and severe complications of lupus. However, the mechanisms for renal damage have not been well elucidated. There are evidences show that glomerular endothelial cells (GECs) are damaged in LN. Immune complexes can deposit in subendothelial area and could affect GEC functio...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Wang L,Law HKW

    更新日期:2019-10-24 00:00:00

  • Transition from cyclosporine-induced renal dysfunction to nephrotoxicity in an in vivo rat model.

    abstract::Cyclosporin A (CsA), a calcineurin inhibitor, remain the cornerstone of immunosuppressive regimens, regardless of nephrotoxicity, which depends on the duration of drug exposure. The mechanisms and biomarkers underlying the transition from CsA-induced renal dysfunction to nephrotoxicity deserve better elucidation, and ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sereno J,Rodrigues-Santos P,Vala H,Rocha-Pereira P,Alves R,Fernandes J,Santos-Silva A,Carvalho E,Teixeira F,Reis F

    更新日期:2014-05-20 00:00:00

  • Key Developments in Ionic Liquid Crystals.

    abstract::Ionic liquid crystals are materials that combine the classes of liquid crystals and ionic liquids. The first one is based on the multi-billion-dollar flat panel display industry, whilst the latter quickly developed in the past decades into a family of highly-tunable non-volatile solvents. The combination yields materi...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Alvarez Fernandez A,Kouwer PH

    更新日期:2016-05-16 00:00:00

  • Laser induced C(60) cage opening studied by semiclassical dynamics simulation.

    abstract::Laser induced opening of the C(60) cage is studied by a semiclassical electron-radiation-ion dynamics technique. The simulation results indicate that the C(60) cage is abruptly opened immediately after laser excitation. The opening of the C(60) cage induces a quick increase in kinetic energy and a sharp decrease in el...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Tang H,Li H,Dou Y

    更新日期:2011-01-13 00:00:00

  • Alterations of Fatty Acid Profile May Contribute to Dyslipidemia in Chronic Kidney Disease by Influencing Hepatocyte Metabolism.

    abstract::Chronic kidney disease (CKD) is associated with atherogenic dyslipidemia. Our aim was firstly to investigate patterns of fatty acids (FA) composition through various stages of CKD, and secondly, to evaluate the effect of CKD-specific FA disturbances on the expression of genes related to lipid metabolism at a cellular ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Czumaj A,Śledziński T,Carrero JJ,Stepnowski P,Sikorska-Wisniewska M,Chmielewski M,Mika A

    更新日期:2019-05-18 00:00:00

  • Role of the Balance of Akt and MAPK Pathways in the Exercise-Regulated Phenotype Switching in Spontaneously Hypertensive Rats.

    abstract::The mechanisms regulating vascular smooth muscle cell (VSMC) phenotype switching and the critical signal modulation affecting the VSMCs remain controversial. Physical exercise acts as an effective drug in preventing elevated blood pressure and improving vascular function. This study was designed to explore the influen...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Zhang L,Zhang Y,Wu Y,Yu J,Zhang Y,Zeng F,Shi L

    更新日期:2019-11-13 00:00:00

  • Differences in the structure of the gut bacteria communities in development stages of the Chinese white pine beetle (Dendroctonus armandi).

    abstract::The Chinese white pine beetle Dendroctonus armandi Tsai and Li, is arguably the most destructive forest insect in the Qinling Mountains in Northern China. Little is known about the structure of the bacterial communities associated with D. armandi even though this wood-boring insect plays important roles in ecosystem a...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Hu X,Wang C,Chen H,Ma J

    更新日期:2013-10-18 00:00:00

  • GroEL-assisted protein folding: does it occur within the chaperonin inner cavity?

    abstract::The folding of protein molecules in the GroEL inner cavity under the co-chaperonin GroES lid is widely accepted as a crucial event of GroEL-assisted protein folding. This review is focused on the data showing that GroEL-assisted protein folding may proceed out of the complex with the chaperonin. The models of GroEL-as...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Marchenkov VV,Semisotnov GV

    更新日期:2009-05-12 00:00:00

  • Immunomodulation Induced by Stem Cell Mobilization and Harvesting in Healthy Donors: Increased Systemic Osteopontin Levels after Treatment with Granulocyte Colony-Stimulating Factor.

    abstract::Peripheral blood stem cells from healthy donors mobilized by granulocyte colony-stimulating factor (G-CSF) and harvested by leukapheresis are commonly used for allogeneic stem cell transplantation. The frequency of severe graft versus host disease is similar for patients receiving peripheral blood and bone marrow allo...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Melve GK,Ersvaer E,Akkök ÇA,Ahmed AB,Kristoffersen EK,Hervig T,Bruserud Ø

    更新日期:2016-07-19 00:00:00

  • Exogenous Glycinebetaine Reduces Cadmium Uptake and Mitigates Cadmium Toxicity in Two Tobacco Genotypes Differing in Cadmium Tolerance.

    abstract::Greenhouse hydroponic experiments were conducted using Cd-sensitive (cv. Guiyan1) and Cd-tolerant (cv. Yunyan2) tobacco cultivars to study the ameliorative effects of exogenous glycinebetaine (GB) upon 5 μM Cd stress. The foliar spray of GB markedly reduced Cd concentrations in plants and alleviated Cd-induced soil pl...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: He X,Richmond MEA,Williams DV,Zheng W,Wu F

    更新日期:2019-03-31 00:00:00

  • Exogenous GA₃ Application Enhances Xylem Development and Induces the Expression of Secondary Wall Biosynthesis Related Genes in Betula platyphylla.

    abstract::Gibberellin (GA) is a key signal molecule inducing differentiation of tracheary elements, fibers, and xylogenesis. However the molecular mechanisms underlying the effect of GA on xylem elongation and secondary wall development in tree species remain to be determined. In this study, Betula platyphylla (birch) seeds wer...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Guo H,Wang Y,Liu H,Hu P,Jia Y,Zhang C,Wang Y,Gu S,Yang C,Wang C

    更新日期:2015-09-23 00:00:00