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Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes.

Abstract：

:The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media were supplemented with 10 mIU/ml FSH for 24 h (group 1), 10 mIU/ml FSH for 2 h (group 2), 75 mIU/ml FSH for 24 h (group 3) or 75 mIU/ml FSH for 2 h (group 4). In each group, half of the in-vitro-matured oocytes were fertilized and cultured to blastocysts and the remaining matured oocytes were analysed for growth differentiation factor (GDF)-9 and bone morphogenetic protein (BMP)-15 mRNA to assess the oocyte quality. The maturation rates and oocyte BMP-15 mRNA concentrations were similar among the four groups. The GDF-9 mRNA concentrations were similar in group 2 and group 4. The fertilization and blastocyst rates were higher in groups 2 and 4 than in groups 1 and 3. It is concluded that 2-h incubation with FSH is better than 24-h incubation in terms of the fertilization rate and blastocyst development.

journal_name

Reprod Biomed Online

journal_title

Reproductive biomedicine online

authors

Lin YH,Hwang JL,Seow KM,Huang LW,Hsieh BC,Chen HJ,Tzeng CR,Bai CH

doi

10.1016/j.rbmo.2011.03.019

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

111-7

issue

1

eissn

1472-6483

issn

1472-6491

pii

S1472-6483(11)00175-1

journal_volume

23

pub_type

杂志文章

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