当前位置： SCI文献检索 > REPRODUCTIVE BIOMEDICINE ONLINE期刊下所有文献 > GnRH agonist and GnRH antagonist protocols in ovarian stimulation: differential regulation pathway of aromatase expression in human granulosa cells.

GnRH agonist and GnRH antagonist protocols in ovarian stimulation: differential regulation pathway of aromatase expression in human granulosa cells.

Abstract：

:Gonadotrophin-releasing hormone (GnRH) agonists and antagonists have been widely used to prevent premature LH surge during ovarian stimulation. However, studies have shown a significantly lower serum oestradiol concentration on the day of human chorionic gonadotrophin administration for cycles using GnRH antagonist. This study compared aromatase gene expression in granulosa lutein cells from 50 women randomly assigned to receive either GnRH agonist (group 1, n=28) or GnRH antagonist (group 2, n=22). The cellular mechanism involved in the observed effects was also investigated. GnRH antagonist treatment significantly affected serum oestradiol concentration (1894+/-138 versus 1074+/-63 pg/ml; P < or = 0.001), follicular-fluid oestradiol concentration in large follicles (18,565+/-2467 versus 10,184+/-1993 pg/ml; P < or = 0.05), aromatase activity (9600+/-1179 versus 5376+/-997 fmol/10(6) cells/h; P < or = 0.05) and mRNA aromatase/mRNA glyceraldehyde 3-phosphate dehydrogenase (15+/-3 versus 6+/-1; P < 0.05). Protein kinase C (PKC) activity in granulosa lutein cells from the GnRH antagonist group was 2.5-fold higher than in the GnRH agonist group. In-vitro experiments showed that selective down-regulation of PKC was only observed in GnRH-desensitized granulosa lutein cells. This report suggests that, in granulosa lutein cells, the modulation of the FSH-induced protein kinase A pathway by PKC was different in agonist versus antagonist cycles.

journal_name

Reprod Biomed Online

journal_title

Reproductive biomedicine online

authors

Khalaf M,Mittre H,Levallet J,Hanoux V,Denoual C,Herlicoviez M,Bonnamy PJ,Benhaim A

doi

10.1016/j.rbmo.2010.03.017

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

56-65

issue

1

eissn

1472-6483

issn

1472-6491

pii

S1472-6483(10)00168-9

journal_volume

21

pub_type

杂志文章,随机对照试验

相关文献

REPRODUCTIVE BIOMEDICINE ONLINE文献大全
  • Effect of human embryonic morphological development on fetal growth parameters: the Rotterdam Periconceptional Cohort (Predict Study).

    abstract：RESEARCH QUESTION:Is embryonic morphological development according to the Carnegie stages associated with pregnancy outcome? DESIGN:In a tertiary hospital-based cohort, 182 singleton non-malformed pregnancies were selected. Serial transvaginal three-dimensional ultrasound (3D-US) scans were carried out between 6+0 and...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2018.12.016

    authors： Parisi F,Rousian M,Koning AHJ,Willemsen SP,Steegers EAP,Steegers-Theunissen RPM

    更新日期：2019-04-01 00:00:00

  • mTOR/P70S6K promotes spermatogonia proliferation and spermatogenesis in Sprague Dawley rats.

    abstract：:Spermatogenesis is a critical process for maintaining male fertility. Sustained spermatogonial stem cell self-renewal and differentiation ensures constant spermatogenesis, and several signalling pathways regulate this process. An increasing number of studies have suggested that the mammalian target of rapamycin (mTOR)...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2015.11.007

    authors： Xu H,Shen L,Chen X,Ding Y,He J,Zhu J,Wang Y,Liu X

    更新日期：2016-02-01 00:00:00

  • Low oxygen tension during IVM improves bovine oocyte competence and enhances anaerobic glycolysis.

    abstract：:This study evaluated the effect of two oxygen concentrations (20 and 5%) on bovine embryo development (kinetics of first cleavage and blastocyst development) during maturation (M) and fertilization (F) and analysed differences in gene expression between cumulus-oocyte complexes (COC) matured at 5 or 20% oxygen and the...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2009.12.006

    authors： Bermejo-Alvarez P,Lonergan P,Rizos D,Gutiérrez-Adan A

    更新日期：2010-03-01 00:00:00

  • The impact of music therapy on pain and stress reduction during oocyte retrieval - a randomized controlled trial.

    abstract：RESEARCH QUESTION:Does music therapy help in reducing pain and anxiety in women undergoing transvaginal ultrasound-guided oocyte retrieval (TUGOR)? DESIGN:In this randomized controlled open label study, 209 participants were recruited and randomized into three groups (music group, n = 70; headphone group, n = 70; cont...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.rbmo.2018.04.049

    authors： Cheung CWC,Yee AWW,Chan PS,Saravelos SH,Chung JPW,Cheung LP,Kong GWS,Li TC

    更新日期：2018-08-01 00:00:00

  • Over 900 oocyte cryopreservation babies born with no apparent increase in congenital anomalies.

    abstract：:Over the past decade, the number of reported live births resulting from oocyte cryopreservation has rapidly increased. To appreciate the true number of children born, verified live births were tabulated and assessed. A literature search was performed; authors were then contacted to verify birth outcomes and provide up...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)60025-9

    authors： Noyes N,Porcu E,Borini A

    更新日期：2009-06-01 00:00:00

  • Prevention of intra-peritoneal adhesions in gynaecological surgery: theory and evidence.

    abstract：:Post-operative adhesions are a significant complication of all abdominal surgical procedures. The major strategies for adhesion prevention in gynaecological surgery are focused on the optimization of surgical technique and use of anti-adhesive agents, which fall into two main categories: pharmacological agents and bar...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,评审

    doi：10.1016/j.rbmo.2010.04.021

    authors： Pados G,Venetis CA,Almaloglou K,Tarlatzis BC

    更新日期：2010-09-01 00:00:00

  • Screening for mutations of the FOXO4 gene in premature ovarian failure patients.

    abstract：:FOXO4 constitutes a coherent candidate gene associated with premature ovarian failure (POF) pathogenesis. This study sequenced the coding and exon-flanking regions of this gene in a panel of 116 POF patients and 143 controls of Tunisian origin. In both groups, the IVS2 + 41T > G sequence variant was identified. It is ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2011.11.017

    authors： Fonseca DJ,Garzón E,Lakhal B,Braham R,Ojeda D,Elghezal H,Saâd A,Restrepo CM,Laissue P

    更新日期：2012-03-01 00:00:00

  • Obesity does not alter endometrial gene expression in women with endometriosis.

    abstract：RESEARCH QUESTION:Does obesity affect endometrial gene expression in women with endometriosis, specifically women with stage I disease? DESIGN:Differential gene expression analysis was conducted on endometrium from women with and without endometriosis (n = 169). Women were diagnosed after surgical visualization and st...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2020.03.015

    authors： Holdsworth-Carson SJ,Chung J,Sloggett C,Mortlock S,Fung JN,Montgomery GW,Dior UP,Healey M,Rogers PA,Girling JE

    更新日期：2020-07-01 00:00:00

  • Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes.

    abstract：:The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2011.03.019

    authors： Lin YH,Hwang JL,Seow KM,Huang LW,Hsieh BC,Chen HJ,Tzeng CR,Bai CH

    更新日期：2011-07-01 00:00:00

  • Truths and myths of oocyte sensitivity to controlled rate freezing.

    abstract：:The mammalian oocyte is especially sensitive to cryopreservation. Because of its size and physiology, it can easily undergo cell death or sub-lethal damage as a consequence of intracellular ice formation, increase in the concentration of solutes and other undesired effects during the conversion of extracellular water ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,评审

    doi：10.1016/s1472-6483(10)60687-6

    authors： Coticchio G,Bonu MA,Sciajno R,Sereni E,Bianchi V,Borini A

    更新日期：2007-07-01 00:00:00

  • Preimplantation diagnosis for neurofibromatosis.

    abstract：:Preimplantation genetic diagnosis (PGD) has recently been performed for inherited cancer predisposition determined by p53 tumour suppressor gene mutations, suggesting the usefulness of PGD for late onset disorders with genetic predisposition, including those caused by the germline mutations of other tumour suppressor ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)61809-3

    authors： Verlinsky Y,Rechitsky S,Verlinsky O,Chistokhina A,Sharapova T,Masciangelo C,Levy M,Kaplan B,Lederer K,Kuliev A

    更新日期：2002-05-01 00:00:00

  • Luteal phase characteristics following GnRH antagonist or agonist treatment - a comparative study.

    abstract：:Due to inherent differences between gonadotrophin-releasing hormone (GnRH) antagonists and agonists, their late effect on ovarian steroidal production during the luteal phase of IVF cycles may differ. The aim of this study was to characterize and compare the luteal phase hormonal profile after the use of GnRH antagoni...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/s1472-6483(10)60976-5

    authors： Friedler S,Gilboa S,Schachter M,Raziel A,Strassburger D,Ron El R

    更新日期：2006-01-01 00:00:00

  • Screening oocytes by polar body biopsy.

    abstract：:Preimplantation genetic aneuploidy screening performed by polar body biopsy has become a frequently used method, especially as in several countries only preconceptional genetic diagnosis is allowed. To penetrate the zona pellucida, mechanical, chemical and laser-assisted techniques have been introduced. In this paper,...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,评审

    doi：10.1016/s1472-6483(10)62023-8

    authors： Dawson A,Griesinger G,Diedrich K

    更新日期：2006-07-01 00:00:00

  • Characterization of early pregnancy placental progesterone production by use of dydrogesterone in programmed frozen-thawed embryo transfer cycles.

    abstract：RESEARCH QUESTION:When and how does the gradual transition of the endocrine control of early pregnancy from the corpus luteum to the placenta, termed luteoplacental shift, take place? DESIGN:Prospective analysis of serum progesterone levels in pregnancies (n = 88) resulting from programmed frozen-thawed embryo transfe...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2020.01.019

    authors： Neumann K,Depenbusch M,Schultze-Mosgau A,Griesinger G

    更新日期：2020-05-01 00:00:00

  • Efficacy of IVF using frozen donor semen in cases of previously failed DI cycles compared with tubal infertility: a cohort study.

    abstract：:A cohort follow-up study was designed to compare the efficacy of IVF using frozen donor semen (IVF-D) following previously failed DI cycles (unexplained female infertility) and direct IVF-D treatment because of tubal infertility (control group). The cohort comprised 189 couples initiating IVF-D after previously failed...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)61275-8

    authors： Guerif F,Saussereau MH,Barthelemy C,Couet ML,Gervereau O,Lansac J,Royere D

    更新日期：2004-10-01 00:00:00

  • Removal of cervical mucus: effect on pregnancy rates in IVF/ICSI.

    abstract：:Cervical mucus may cover the embryo transfer catheter during passage of the cervical canal, interfering with the correct placement of the embryo(s) into the uterine cavity. The effect of removal of cervical mucus prior to embryo transfer in IVF/ intracytoplasmic sperm injection (ICSI) on live birth rate was studied. T...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/s1472-6483(10)60344-6

    authors： Visschers BA,Bots RS,Peeters MF,Mol BW,van Dessel HJ

    更新日期：2007-09-01 00:00:00

  • Intended parents' motivations and information and support needs when seeking extraterritorial compensated surrogacy.

    abstract：:Cross-border reproductive care (CBRC) is becoming increasingly common. Little is known about the motivations and information and support needs of people who cross borders to access surrogacy. This study aimed to explore: how those considering or undertaking extraterritorial surrogacy reach their decision; what other a...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2015.08.008

    authors： Hammarberg K,Stafford-Bell M,Everingham S

    更新日期：2015-11-01 00:00:00

  • Morphokinetic parameters of ICSI tripronucleated embryos observed using time lapse.

    abstract：:Time-lapse analysis of tripronucleated zygotes obtained in ICSI cycles showed that 75.4% cleaved into embryos. These embryos subsequently demonstrated slower developmental kinetics than normally fertilized embryos. ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2014.01.009

    authors： Lammers J,Splingart C,Barrière P,Fréour T

    更新日期：2014-05-01 00:00:00

  • Glucose/lactate metabolism of cryopreserved intact bovine ovaries as a novel quantitative marker to assess tissue cryodamage.

    abstract：:For some patients, the autotransplantation of a cryopreserved-thawed intact ovary might be the best option to preserve their reproductive potential after fertility-threatening treatment. The best procedure to successfully cryopreserve a human ovary without inflicting a devastating level of cryodamage is to date unknow...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2011.08.008

    authors： Gerritse R,Beerendonk CC,Westphal JR,Bastings L,Braat DD,Peek R

    更新日期：2011-12-01 00:00:00

  • First pregnancy and life after preimplantation genetic diagnosis by polar body analysis for mucopolysaccharidosis type I.

    abstract：:Preimplantation genetic diagnosis (PGD) may help couples at risk to avoid pregnancies with known genetic diseases. In Germany, the only option to perform PGD is the analysis of polar bodies (PB). Mucopolysaccharidosis type I (MPS I) is an autosomal recessive lysosomal storage disorder. Q70X is one of the frequent dise...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)60864-4

    authors： Tomi D,Schultze-Mosgau A,Eckhold J,Schopper B,Al-Hasani S,Steglich C,Gal A,Axt-Fliedner R,Schwinger E,Diedrich K,Griesinger G

    更新日期：2006-02-01 00:00:00

  • Novel cause of hereditary obstructive azoospermia: a T2 allele in the CFTR gene.

    abstract：:Congenital bilateral absence of the vas deferens (CBAVD) is a frequent cause of obstructive azoospermia, and caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. A novel TG(13)T(2) allele was identified in a CBAVD patient with no clinical cystic fibrosis phenotype, normal pancrea...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)60089-2

    authors： Radpour R,Taherzadeh-Fard E,Gourabi H,Aslani S,Vosough Dizaj A,Aslani A

    更新日期：2009-03-01 00:00:00

  • Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique.

    abstract：:This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated. The cryoprotectant solution was chosen after histological evaluation of mouse and human ...

    journal_title：Reproductive biomedicine online

    pub_type： 历史文章,杂志文章

    doi：10.1016/j.rbmo.2010.10.015

    authors： Rosendahl M,Schmidt KT,Ernst E,Rasmussen PE,Loft A,Byskov AG,Andersen AN,Andersen CY

    更新日期：2011-02-01 00:00:00

  • Embryo development characteristics in Robertsonian and reciprocal translocations: a comparison of results with non-translocation cases.

    abstract：:The effect of translocations on embryo development was evaluated and results were compared in terms of embryo development with those of embryos obtained from standard intracytoplasmic sperm injection (ICSI) cycles. In 23 translocation carriers with 34 cycles, fertilization, pronuclear morphology scoring (PMS), develop...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)62073-1

    authors： Findikli N,Kahraman S,Kumtepe Y,Donmez E,Biricik A,Sertyel S,Berkil H,Melil S

    更新日期：2003-11-01 00:00:00

  • Outcome of early versus late multifetal pregnancy reduction.

    abstract：:Multifetal pregnancy reduction (MPR) is an accepted method of reducing complications of triplet pregnancies and higher-order multifetal pregnancies. Eighty-three pregnancies that underwent early (68 weeks) transvaginal MPR were compared with 125 pregnancies that underwent late (11-14 weeks) transabdominal MPR. Rates o...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2016.08.015

    authors： Haas J,Barzilay E,Hourvitz A,Dor J,Lipitz S,Yinon Y,Shlomi M,Shulman A

    更新日期：2016-11-01 00:00:00

  • Comparative assessment of the consistency and quality of a highly purified FSH extracted from human urine (urofollitropin) and a recombinant human FSH (follitropin alpha).

    abstract：:The revolutionary development of biotechnology-derived therapeutic proteins has provided the expected improvements in quality, purity and consistency, as demonstrated in recombinant human FSH (rhFSH). However, the development of urine-derived gonadotrophins has not always shown comparable improvements. More recently, ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)60613-x

    authors： Lispi M,Bassett R,Crisci C,Mancinelli M,Martelli F,Ceccarelli D,De Bellis C,Mendola D

    更新日期：2006-08-01 00:00:00

  • Extended embryo culture to increase implantation rate.

    abstract：:Multiple pregnancies are a serious adverse event of IVF treatment. Several strategies have been developed to improve embryo selection and embryo developmental potential to increase pregnancy rates without increasing the risk of multiple pregnancies. The only way to effectively solve this problem is to reduce the numbe...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/S1472-6483(10)61459-9

    authors： Quea G,Romero K,Garcia-Velasco JA

    更新日期：2007-01-01 00:00:00

  • A technical note on the assessment of human sperm vitality using eosin-nigrosin staining.

    abstract：RESEARCH QUESTION:How many spermatozoa and slides need to be counted to make a reliable assessment of sperm vitality? Currently, various authorities recommend assessing human sperm vitality on counts of at least 200 cells, but on one or two slides. DESIGN:This was an observational study on duplicate eosin-nigrosin sta...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2020.03.002

    authors： Mortimer D

    更新日期：2020-06-01 00:00:00

  • Different preferences for prenatal diagnosis in pregnancies following assisted reproduction versus spontaneous conception.

    abstract：:The uptake of prenatal diagnosis in 436 singleton and 146 twin pregnancies following assisted reproduction was compared with a matched group of spontaneous conceptions. The first and second trimester ultrasound examination included target fetal anatomic evaluation and screening by specific markers described for fetal ...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/s1472-6483(10)60506-8

    authors： Geipel A,Berg C,Katalinic A,Ludwig M,Germer U,Diedrich K,Gembruch U

    更新日期：2004-01-01 00:00:00

  • Systematic review of the clinical efficacy of vaginal progesterone for luteal phase support in assisted reproductive technology cycles.

    abstract：:Vaginal progesterone via capsule, gel or tablet is the most common route for luteal phase support (LPS) in Europe. Although there is a wealth of data comparing products used at other stages of assisted reproductive technology cycles, there is a lack of systematically identified evidence comparing the wide range of vag...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章,评审

    doi：10.1016/j.rbmo.2018.02.001

    authors： Child T,Leonard SA,Evans JS,Lass A

    更新日期：2018-06-01 00:00:00

  • Mid-luteal progesterone concentrations are associated with live birth rates during ovulation induction.

    abstract：:This retrospective study investigated whether mid-luteal serum progesterone concentrations are associated with live birth rates in women with WHO group II anovulatory infertility undergoing ovulation induction. Data were from women (n=335) stimulated with gonadotrophins using a low-dose step-up protocol, of which wome...

    journal_title：Reproductive biomedicine online

    pub_type： 杂志文章

    doi：10.1016/j.rbmo.2011.01.006

    authors： Arce JC,Balen A,Platteau P,Pettersson G,Andersen AN

    更新日期：2011-05-01 00:00:00