Abstract:
RESEARCH QUESTION:Which cryopreservation method better protects reproductive potential: the cryopreservation of a testicular cell suspension (TCS) or the cryopreservation of testicular tissue (TET)? DESIGN:Two cryopreservation strategies for spermatogonial stem cells (SSCs) were compared in a mouse model: cryopreservation as TET or as TCS. Evaluated outcomes were number of viable cells after thawing, number and length of donor-derived colonies after spermatogonial stem cell transplantation (SSCT), number of litters, litter size and number of donor-derived pups after mating. RESULTS:Compared with cryopreserving TCS, cryopreservation of TET resulted in significantly higher numbers of viable cells after thawing (TET: 13.4 × 104 ± 7.2 × 104 versus TCS: 8.2 × 104 ± 2.7 × 104; P = 0.0002), more (TET: 47.6 ± 19.2 versus TCS: 18.5 ± 13.0; P = 0.0039) and longer (TET: 5.2 ± 1.0 mm versus TCS: 2.7 ± 1.5 mm; P = 0.0016) donor-derived colonies, and more donor-derived pups per litter (TET: 2.2 ± 0.2 versus TCS: 0.5 ± 0.1; P = 0.0008). CONCLUSIONS:Cryopreservation of TET is the preferred method to cryopreserve SSCs prior to SSCT in a mouse model.
journal_name
Reprod Biomed Onlinejournal_title
Reproductive biomedicine onlineauthors
Onofre J,Kadam P,Baert Y,Goossens Edoi
10.1016/j.rbmo.2019.10.016subject
Has Abstractpub_date
2020-02-01 00:00:00pages
261-269issue
2eissn
1472-6483issn
1472-6491pii
S1472-6483(19)30796-5journal_volume
40pub_type
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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journal_title:Reproductive biomedicine online
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