A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage.

Abstract:

:Artificial oocyte activation (AOA) has been previously suggested as a means to overcome the problem of total fertilization failure, which affects about 1-3% of the intracytoplasmic sperm injection (ICSI) cycles. A preliminary study on the application of chemical AOA was conducted using A23187 Ca(2+) ionophore to improve embryonic development in four women with a history of complete fertilization arrest and inability to transit to cleavage stage during previous ICSI trials. Data indicated that activated oocytes resulted in better fertilization, embryonic development and clinical pregnancy in one of the four couples. Therefore, ICSI combined with AOA using Ca(2+) ionophore may be useful in selected patients with cleavage failure, and may help the zygotes to reach more advanced developmental stages.

journal_name

Reprod Biomed Online

authors

Darwish E,Magdi Y

doi

10.1016/j.rbmo.2015.08.012

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

799-804

issue

6

eissn

1472-6483

issn

1472-6491

pii

S1472-6483(15)00420-4

journal_volume

31

pub_type

临床试验,杂志文章
  • Environmental pollutants and lifestyle factors induce oxidative stress and poor prenatal development.

    abstract::Developmental toxicity caused by exposure to a mixture of environmental pollutants has become a major health concern. Human-made chemicals, including xenoestrogens, pesticides and heavy metals, as well as unhealthy lifestyle behaviours, mainly tobacco smoking, alcohol consumption and medical drug abuse, are major fact...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审

    doi:10.1016/j.rbmo.2014.03.002

    authors: Al-Gubory KH

    更新日期:2014-07-01 00:00:00

  • Is the dimension of ovarian endometriomas significantly modified by IVF-ICSI cycles?

    abstract::Information regarding the growth and development of endometriomas during IVF-intracytoplasmic sperm injection (ICSI) cycles is lacking. In this study, the aim was to estimate the influence of IVF-ICSI on the dimension of these cysts and to assess whether this treatment contributes to the manifestation of new endometri...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60099-5

    authors: Benaglia L,Somigliana E,Vighi V,Nicolosi AE,Iemmello R,Ragni G

    更新日期:2009-03-01 00:00:00

  • Supplementation of clomiphene citrate cycles with Cimicifuga racemosa or ethinyl oestradiol--a randomized trial.

    abstract::The anti-oestrogenic activity of clomiphene citrate (CC) on the cervical mucous and endometrium may be the reason for the relatively low pregnancy rates in CC induction cycles. Various follicular-phase supplements have been tried to improve cycle outcome in these patients. This study compared follicular-phase suppleme...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.rbmo.2009.06.007

    authors: Shahin AY,Ismail AM,Shaaban OM

    更新日期:2009-10-01 00:00:00

  • Gonadotrophins or clomiphene citrate in couples with unexplained infertility undergoing intrauterine insemination: a cost-effectiveness analysis.

    abstract:RESEARCH QUESTION:What is the cost-effectiveness of gonadotrophins compared with clomiphene citrate in couples with unexplained subfertility undergoing intrauterine insemination (IUI) with ovarian stimulation under strict cancellation criteria? DESIGN:A cost-effectiveness analysis alongside a randomized controlled tri...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2019.09.004

    authors: Danhof NA,van Wely M,Repping S,van der Ham DP,Klijn N,Janssen ICAH,Rijn-van Weert JM,Twisk M,Traas MAF,Pelinck MJ,Perquin DAM,Boks DES,Sluijmer A,Mol BWJ,van der Veen F,Mochtar MH,Amsterdam SUPER Study Group.

    更新日期:2020-01-01 00:00:00

  • Development and characterization of an endometrial tissue culture system.

    abstract::A culture system has been developed for pieces of whole endometrium, comprising many different cell types. The system maintains the integrity of all compartments important for tissue function and allows functional studies of the human endometrium. Endometrial biopsy strips were collected from fertile women. The strips...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)61759-2

    authors: Stavreus-Evers A,Hovatta O,Eriksson H,Landgren BM

    更新日期:2003-09-01 00:00:00

  • Micromanipulation of the human oocyte.

    abstract::Intracytoplasmic sperm injection (ICSI) provides an excellent outcome in a consistent manner, and is therefore used worldwide as a routine procedure. Since its introduction, few modifications have been made to its methodology. Recently, a combination of ICSI with micro-hole drilling by laser (LA-ICSI) of the zona pell...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)62085-8

    authors: Nagy ZP

    更新日期:2003-12-01 00:00:00

  • Free thiols in human spermatozoa: are Na+/K+-ATPase, Ca2+-ATPase activities involved in sperm motility through peroxynitrite formation?

    abstract::The aim of the present study was to measure free thiols content, Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities in human spermatozoa of asthenozoospermic patients and normospermic donors, and evaluate any influence on kinetic sperm features, as well as correlation with peroxynitrite. In fact, membrane integrity and it...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60435-x

    authors: Vignini A,Buldreghini E,Nanetti L,Amoroso S,Boscaro M,Ricciardo-Lamonica G,Mazzanti L,Balercia G

    更新日期:2009-01-01 00:00:00

  • Redefining advanced maternal age as an indication for preimplantation genetic screening.

    abstract::In this retrospective study, the utility of preimplantation genetic screening (PGS) in patients with advanced maternal age is evaluated. The patient population consisted of women aged 38-44years and included in a regular IVF programme with or without PGS analysis. Transfer rate, ongoing implantation rate and ongoing p...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2010.06.020

    authors: Milán M,Cobo AC,Rodrigo L,Mateu E,Mercader A,Buendía P,Peinado V,Delgado A,Mir P,Simón C,Remohí J,Pellicer A,Rubio C

    更新日期:2010-11-01 00:00:00

  • Symposium: Update on prediction and management of OHSS. Optimal dose of HCG for final oocyte maturation in IVF cycles: absence of evidence?

    abstract::There is an ongoing debate over the optimal dose of urinary HCG (u-HCG) that can trigger final oocyte maturation, leading to higher IVF success rate without increasing the risk of ovarian hyperstimulation syndrome (OHSS). A systematic review was conducted of all studies that compared the effect of at least two doses o...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审

    doi:10.1016/s1472-6483(10)60045-4

    authors: Tsoumpou I,Muglu J,Gelbaya TA,Nardo LG

    更新日期:2009-07-01 00:00:00

  • Results of assisted reproduction techniques in Latin America.

    abstract::The fact that today it is possible to write a comprehensive report on assisted reproduction technologies in Latin America is the result of a serious and systematic effort, accomplished by more than 80 centres, from Mexico to Chile. Over the past 10 years, these centres representing the vast majority of assisted reprod...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)62237-7

    authors: Zegers-Hochschild F,Mackenna A,Fernández E,Sepúlveda MS

    更新日期:2001-01-01 00:00:00

  • AQP7 mediates post-menopausal lipogenesis in adipocytes through FSH-induced transcriptional crosstalk with AP-1 sites.

    abstract:RESEARCH QUESTION:Fat accumulation is present in most post-menopausal women, but the underlying mechanism remains unclear. Aquaporin 7 (AQP7) is the most important glycerol channel facilitating glycerol efflux in adipocytes. High circulating FSH in post-menopausal women may play an independent role in regulation of the...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2020.08.008

    authors: Chen L,Chen H,Liu X,Li J,Gao Q,Shi S,Wang T,Ye X,Lu Y,Zhang D,Sheng J,Jin L,Huang H

    更新日期:2020-12-01 00:00:00

  • Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique.

    abstract::This paper presents the Danish 10-year experience (1999-2009) with cryopreservation (n=386) and autotransplantation of ovarian tissue (n=18). Before applying the technique to humans, the method was thoroughly tested and validated. The cryoprotectant solution was chosen after histological evaluation of mouse and human ...

    journal_title:Reproductive biomedicine online

    pub_type: 历史文章,杂志文章

    doi:10.1016/j.rbmo.2010.10.015

    authors: Rosendahl M,Schmidt KT,Ernst E,Rasmussen PE,Loft A,Byskov AG,Andersen AN,Andersen CY

    更新日期:2011-02-01 00:00:00

  • Anogenital distance in newborn infants conceived by assisted reproduction and natural conception.

    abstract:RESEARCH QUESTION:Does anogenital distance (AGD) differ in newborn infants conceived through assisted reproduction technology (ART) compared with those conceived naturally? DESIGN:This case-control study looked at anthropometric and anogenital measurements in 247 male and 200 female newborns born after ART (n = 121) o...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2020.04.019

    authors: Ercin S,Ertas S,Dundar O,Oktem O,Yakin K

    更新日期:2020-09-01 00:00:00

  • Health of children conceived after preimplantation genetic diagnosis: a preliminary outcome study.

    abstract::A preliminary study was conducted on health of children conceived after preimplantation genetic screening and diagnosis (PGD). Forty-nine children were assessed with 66 matched naturally conceived (NC) controls. Primary outcome was neurodevelopmental screening, and secondary outcomes were evidence of other health prob...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60599-8

    authors: Banerjee I,Shevlin M,Taranissi M,Thornhill A,Abdalla H,Ozturk O,Barnes J,Sutcliffe A

    更新日期:2008-03-01 00:00:00

  • Efficiency of assisted oocyte activation as a solution for failed intracytoplasmic sperm injection.

    abstract::Failed fertilization after intracytoplasmic sperm injection (ICSI) can occur due to an oocyte activation defect. In these cases assisted oocyte activation (AOA) may help but efficiency is still unknown. Prior to AOA, the mouse oocyte activation test (MOAT) can be carried out by injecting human spermatozoa into mouse o...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60313-6

    authors: Heindryckx B,De Gheselle S,Gerris J,Dhont M,De Sutter P

    更新日期:2008-11-01 00:00:00

  • Artificial oocyte activation: evidence for clinical readiness.

    abstract::Artificial oocyte activation using Ca(2+)ionophores or similar compounds is a widely applied technique in IVF laboratories. This is all the more interesting as most of the agents aiming for intracellular Ca(2+) increase do not result in physiological Ca(2+) oscillations but much rather cause a single Ca(2+) transient....

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2015.12.004

    authors: Ebner T,Montag M

    更新日期:2016-03-01 00:00:00

  • Luteal phase rescue in high-risk OHSS patients by GnRHa triggering in combination with low-dose HCG: a pilot study.

    abstract::Triggering of final oocyte maturation with gonadotrophin-releasing hormone agonist (GnRHa) has previously been shown to prevent ovarian hyperstimulation syndrome (OHSS), but at the same time a detrimental effect on clinical outcome parameters was usually reported. In this prospective, observational, proof-of-concept s...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60006-5

    authors: Humaidan P

    更新日期:2009-05-01 00:00:00

  • Role of embryo quality in predicting early pregnancy loss following assisted reproductive technology.

    abstract::Early pregnancy loss (EPL) significantly reduces the initial success rate of assisted reproduction treatments and increases the psychological burden on the patient. The aim of the current study was to investigate the association between embryo quality and EPL in IVF. A retrospective study of 1471 women undergoing IVF ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60637-2

    authors: Hourvitz A,Lerner-Geva L,Elizur SE,Baum M,Levron J,David B,Meirow D,Yaron R,Dor J

    更新日期:2006-10-01 00:00:00

  • Systematic review of the clinical efficacy of vaginal progesterone for luteal phase support in assisted reproductive technology cycles.

    abstract::Vaginal progesterone via capsule, gel or tablet is the most common route for luteal phase support (LPS) in Europe. Although there is a wealth of data comparing products used at other stages of assisted reproductive technology cycles, there is a lack of systematically identified evidence comparing the wide range of vag...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审

    doi:10.1016/j.rbmo.2018.02.001

    authors: Child T,Leonard SA,Evans JS,Lass A

    更新日期:2018-06-01 00:00:00

  • mTOR/P70S6K promotes spermatogonia proliferation and spermatogenesis in Sprague Dawley rats.

    abstract::Spermatogenesis is a critical process for maintaining male fertility. Sustained spermatogonial stem cell self-renewal and differentiation ensures constant spermatogenesis, and several signalling pathways regulate this process. An increasing number of studies have suggested that the mammalian target of rapamycin (mTOR)...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2015.11.007

    authors: Xu H,Shen L,Chen X,Ding Y,He J,Zhu J,Wang Y,Liu X

    更新日期:2016-02-01 00:00:00

  • Effects of growth factors and granulosa cell co-culture on in-vitro maturation of oocytes.

    abstract::The maturation medium for in-vitro oocyte maturation is usually supplemented with serum. However, supplementation with serum from pregnant women adversely affects the outcome of in-vitro maturation. The purpose of the study was to assess if growth factors or granulosa cell co-culture could overcome the adverse effects...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60068-5

    authors: Lin YH,Hwang JL,Seow KM,Huang LW,Chen HJ,Tzeng CR

    更新日期:2009-08-01 00:00:00

  • Nuclear transfer for full karyotyping and preimplantation diagnosis for translocations.

    abstract::Preimplantation genetic diagnosis (PGD) for chromosomal disorders is currently performed by interphase fluorescence in-situ hybridization (FISH) analysis. This technique is known to have limitations for detecting some translocations and complete karyotyping, so visualization of chromosomes in single cells is required ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)61836-6

    authors: Verlinsky Y,Cieslak J,Evsikov S,Galat V,Kuliev A

    更新日期:2002-11-01 00:00:00

  • Efficacy of blastocyst transfer after implantation failure.

    abstract::Clinicians who treat unsuccessful couples despite repeated transfers of good quality embryos face a challenge. Among the various strategies that have been described, embryo transfer at the blastocyst stage has been postulated to improve implantation. A prospective non-randomized analysis was performed in 276 IVF patie...

    journal_title:Reproductive biomedicine online

    pub_type: 临床试验,杂志文章

    doi:10.1016/s1472-6483(10)61773-7

    authors: Guerif F,Bidault R,Gasnier O,Couet ML,Gervereau O,Lansac J,Royere D

    更新日期:2004-12-01 00:00:00

  • Effect of maternal and paternal age on pregnancy and miscarriage rates after intrauterine insemination.

    abstract::More than 17,000 intrauterine insemination (lUI) cycles were analysed retrospectively with respect to outcome according to differing aetiologies of infertility. The quantity and motility of spermatozoa in the final preparation used for insemination had a positive effect on the outcome, as classically observed in the p...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60223-4

    authors: Belloc S,Cohen-Bacrie P,Benkhalifa M,Cohen-Bacrie M,De Mouzon J,Hazout A,Ménézo Y

    更新日期:2008-09-01 00:00:00

  • High-magnification selection of spermatozoa prior to oocyte injection: confirmed and potential indications.

    abstract::Intracytoplasmic morphologically selected sperm injection (IMSI) involves the use of differential interference contrast microscopy at high magnification (at least ·6300) to improve the observation of live human spermatozoa (particularly by showing sperm head vacuoles that are not necessarily seen at lower magnificatio...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审

    doi:10.1016/j.rbmo.2013.09.019

    authors: Boitrelle F,Guthauser B,Alter L,Bailly M,Bergere M,Wainer R,Vialard F,Albert M,Selva J

    更新日期:2014-01-01 00:00:00

  • Decisional authority and moral responsibility of patients and clinicians in the context of preimplantation genetic diagnosis.

    abstract::Case reports reveal that clinicians applying preimplantation genetic diagnosis are increasingly confronted with requests by patients which they consider ethically problematic. These requests raise the question to what extent physicians share responsibility for the welfare of the future child. Two categories of situati...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)62068-8

    authors: Pennings G,Bonduelle M,Liebaers I

    更新日期:2003-11-01 00:00:00

  • Overview of the Greek legislation regarding assisted reproduction and comparison with the EU legal framework.

    abstract::The aim of this research was to ascertain how the opportunities now open by the Greek legislation regarding assisted reproduction fits with Greek society and how it compares with the wider EU legal framework. A revision of the Greek legislation took place a few years ago, with two new Acts. The different issues that a...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2011.07.024

    authors: Leon G,Papetta A,Spiliopoulou C

    更新日期:2011-12-01 00:00:00

  • Coasting, embryo development and outcomes of blastocyst transfer: a case-control study.

    abstract::This study compared the effect on blastocyst development and clinical outcome of coasting in women at increased risk of moderate-severe ovarian hyperstimulation syndrome (OHSS; n=389) with a control group matched for age and basal FSH that did not undergo coasting (n=386) in IVF/intracytoplasmic sperm injection (ICSI)...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2014.04.014

    authors: Talebi Chahvar S,Zosmer A,Caragia A,Balestrini S,Sabatini L,Tranquilli AL,Al-Shawaf T

    更新日期:2014-08-01 00:00:00

  • Stem cell research policies: who's afraid of complicity?

    abstract::Countries that oppose embryo destruction for research purposes can forbid the production of human embryonic stem cell lines on their territory, but these cell lines are nevertheless produced abroad. Therefore, these countries also need to determine if their position on embryo destruction allows them to import and use ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/s1472-6483(10)60063-6

    authors: Mertes H,Pennings G

    更新日期:2009-01-01 00:00:00

  • Effects of treatment of ectopic pregnancy with methotrexate or salpingectomy in the subsequent IVF cycle.

    abstract::Ectopic pregnancy is a known risk for patients treated with IVF. The objective of this study was to evaluate the effect of methotrexate (MTX) and laparoscopic salpingectomy as treatments of ectopic pregnancy on ovarian response during IVF cycles. Data of all women treated for ectopic pregnancy as a result of IVF treat...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章

    doi:10.1016/j.rbmo.2013.01.018

    authors: Wiser A,Gilbert A,Nahum R,Orvieto R,Haas J,Hourvitz A,Weissman A,Younes G,Dirnfeld M,Hershko A,Shulman A,Tsafrir A,Holzer H,Shalom-Paz E,Tulandi T

    更新日期:2013-05-01 00:00:00