A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage.


:Artificial oocyte activation (AOA) has been previously suggested as a means to overcome the problem of total fertilization failure, which affects about 1-3% of the intracytoplasmic sperm injection (ICSI) cycles. A preliminary study on the application of chemical AOA was conducted using A23187 Ca(2+) ionophore to improve embryonic development in four women with a history of complete fertilization arrest and inability to transit to cleavage stage during previous ICSI trials. Data indicated that activated oocytes resulted in better fertilization, embryonic development and clinical pregnancy in one of the four couples. Therefore, ICSI combined with AOA using Ca(2+) ionophore may be useful in selected patients with cleavage failure, and may help the zygotes to reach more advanced developmental stages.


Reprod Biomed Online


Darwish E,Magdi Y




Has Abstract


2015-12-01 00:00:00














  • What fertility specialists should know about the vaginal microbiome: a review.

    abstract::Our understanding of the composition of the microbial communities that inhabit the human body, known as the 'microbiome', is aided by the development of non-culture-dependent DNA sequencing. It is increasingly apparent that the balance of microbial species greatly affects the health of the host. Disturbances in the co...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: García-Velasco JA,Menabrito M,Catalán IB

    更新日期:2017-07-01 00:00:00

  • Prospective randomized comparison of Wallace and Labotect embryo transfer catheters.

    abstract::The aim of this study was to compare the clinical performance of Wallace and Labotect embryo transfer catheters in a single assisted reproduction centre. A total of 260 women undergoing embryo transfer were randomized between Wallace and Labotect catheters. When both catheters failed to negotiate the cervical canal, t...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Ata B,Isiklar A,Balaban B,Urman B

    更新日期:2007-04-01 00:00:00

  • Failure mode and effects analysis of witnessing protocols for ensuring traceability during IVF.

    abstract::Traceability of cells during IVF is a fundamental aspect of treatment, and involves witnessing protocols. Failure mode and effects analysis (FMEA) is a method of identifying real or potential breakdowns in processes, and allows strategies to mitigate risks to be developed. To examine the risks associated with witnessi...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Rienzi L,Bariani F,Dalla Zorza M,Romano S,Scarica C,Maggiulli R,Nanni Costa A,Ubaldi FM

    更新日期:2015-10-01 00:00:00

  • Vitrification and the demise of fresh treatment cycles in ART.

    abstract::The advent of vitrification has transformed the therapeutic landscape in assisted reproductive technology. Clear evidence for this is provided by the dramatic rise in the number of frozen embryo transfer (FET) cycles being carried out annually. In this review, we examine the reasons that underlie this trend and the cu...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Ahuja KK,Macklon N

    更新日期:2020-08-01 00:00:00

  • Improvement of IVF results and optimisation of quality control by using intermittent activity.

    abstract::For several years the present authors' team had not been satisfied with the pregnancy rate achieved by their unit, especially in comparison with the results achieved by other teams with a similar population. A quality programme was established, and it was decided to switch from non-stop to intermittent activity. It wa...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Frydman N,Fanchin R,Le Dû A,Bourrier MC,Tachdjian G,Frydman R

    更新日期:2004-11-01 00:00:00

  • Effect of FSH and its different isoforms on maturation of oocytes from pre-ovulatory follicles.

    abstract::FSH exists as a family of isohormones exhibiting distinct oligosaccharide structures, and the released FSH isoform mixtures change during the follicular phase of the menstrual cycle. In addition, the pulsatile release of gonadotrophins seems to expose follicles to bursts of more less-acidic FSH isoforms and the follic...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Yding Andersen C

    更新日期:2002-11-01 00:00:00

  • Reproductive strategies for human survival.

    abstract::Reproductive strategies for the human species have basically remained unaltered since Homo sapiens first appeared, probably in the valleys of Africa: males have always attempted to pass their genes to the largest feasible number of females, selecting those females capable of providing the best quality of oocytes; fema...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Benagiano G

    更新日期:2002-01-01 00:00:00

  • The importance of diagnosis in the clinical management of infertility in the male.

    abstract::This paper examines the importance of a clinical diagnosis in the management of infertility in the male and discusses the reasons for this statement. The treatment of male infertility is now often carried out solely by gynaecologists. These specialists have frequently had little training in the examination of the male...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Jequier AM

    更新日期:2006-09-01 00:00:00

  • How old is too old? A contribution to the discussion on age limits for assisted reproduction technique access.

    abstract::In 2012, the Czech Republic established the women's age limit for access to assisted reproduction techniques at age 49 years. In this paper, the acceptability of this age limit from the children's perspective in the Czech Republic is assessed. Although the necessity of balancing the interests of parents and children i...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Kocourková J,Konečná H,Burcin B,Kučera T

    更新日期:2015-05-01 00:00:00

  • Outcome of assisted reproductive technologies after different embryo transfer strategies.

    abstract::The outcomes of 1028 assisted reproductive technology cycles were studied retrospectively, considering two different periods of embryo transfer. In the first period, 262 cycles in women < 36 years old were studied, in which three embryos were transferred, followed by 157 cycles in women > or = 36 years, in which four ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Setti PE,Cavagna M,Albani E,Morreale G,Novara PV,Cesana A,Parini V

    更新日期:2005-07-01 00:00:00

  • History of oocyte cryopreservation.

    abstract::The potential advantages of being able to cryopreserve oocytes have been apparent for many decades. Technical difficulties associated with the unique properties of the mammalian oocyte initially retarded rapid development in this area but recent advances have overcome many of the problems. A stage has now been reached...

    journal_title:Reproductive biomedicine online

    pub_type: 历史文章,杂志文章


    authors: Gook DA

    更新日期:2011-09-01 00:00:00

  • Role of LH in ovarian stimulation: considerations.

    abstract::The role of LH in ovarian stimulation continues to be controversial. Most observational studies on both gonadotrophin-releasing hormone (GnRH) agonist and GnRH antagonist protocols failed to show an effect on ongoing pregnancy likelihood of punctually measured 'low' or 'high' endogenous LH concentrations during ovaria...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Griesinger G,Diedrich K

    更新日期:2006-04-01 00:00:00

  • Sex selection, public policy and the HFEA's role in political decision making - response to Edgar Dahl's 'The presumption in favour of liberty'.

    abstract::The Human Fertilisation and Embryology Authority's report on sex selection is criticized because its recommendation to prohibit all techniques for social sexing is based on unsupported allegations. As a body with a scientific status, the Authority should allow the application of some methods of sex selection for non-m...

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Pennings G

    更新日期:2004-03-01 00:00:00

  • Mutations in HOXA11 are not responsible for Müllerian duct anomalies in Chinese patients.

    abstract::As a member of the homeobox (HOX) gene family, HOXA11 is expressed in the primordia of lower uterus and cervix during fetal life and is essential for endometrial development and embryo implantation in the adults. The aim of the present study was to investigate whether mutations in HOXA11 contribute to Müllerian duct a...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Chen X,Li G,Qin Y,Cui Y,You L,Chen ZJ

    更新日期:2014-06-01 00:00:00

  • Percutaneous aspiration biopsy using an intravenous catheter for testicular sperm retrieval in patients with obstructive azoospermia.

    abstract::Testicular needle aspiration has been proposed as a simple alternative to open biopsy for the diagnosis and treatment of azoospermia. This study describes a new modification of needle aspiration biopsy using an ordinary intravenous catheter to retrieve testicular spermatozoa from patients with obstructive azoospermia,...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Fahmy I,Kamal A,Aboulghar M,Mansour R,Serour GI,Shamloul R

    更新日期:2004-07-01 00:00:00

  • Coasting, embryo development and outcomes of blastocyst transfer: a case-control study.

    abstract::This study compared the effect on blastocyst development and clinical outcome of coasting in women at increased risk of moderate-severe ovarian hyperstimulation syndrome (OHSS; n=389) with a control group matched for age and basal FSH that did not undergo coasting (n=386) in IVF/intracytoplasmic sperm injection (ICSI)...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Talebi Chahvar S,Zosmer A,Caragia A,Balestrini S,Sabatini L,Tranquilli AL,Al-Shawaf T

    更新日期:2014-08-01 00:00:00

  • Fundamentals of cryobiology in reproductive medicine.

    abstract::The aim of this review will be to provide a basic understanding of the biophysical processes that accompany the application of cryopreservation in reproductive medicine. The ability to store cells in 'suspended animation' outside the body has become a keystone practice in the development of many modern clinical therap...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Fuller B,Paynter S

    更新日期:2004-12-01 00:00:00

  • Novel cause of hereditary obstructive azoospermia: a T2 allele in the CFTR gene.

    abstract::Congenital bilateral absence of the vas deferens (CBAVD) is a frequent cause of obstructive azoospermia, and caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. A novel TG(13)T(2) allele was identified in a CBAVD patient with no clinical cystic fibrosis phenotype, normal pancrea...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Radpour R,Taherzadeh-Fard E,Gourabi H,Aslani S,Vosough Dizaj A,Aslani A

    更新日期:2009-03-01 00:00:00

  • Techniques of laparoscopic myomectomy.

    abstract::Myomectomy is one of the commonest gynaecological operations. Laparoscopic myomectomy has emerged over the last two decades as a possible alternative to the traditional laparotomy. Most studies have revealed that the laparoscopic procedure is at least as safe as the open procedure as to the rate of complications and m...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Mattei A,Cioni R,Bargelli G,Scarselli G

    更新日期:2011-07-01 00:00:00

  • Oocyte vitrification technology has made egg-sharing donation easier in China.

    abstract::When infertile women undergoing IVF or intracytoplasmic sperm injection (ICSI) have more than 20 mature oocytes retrieved, at least 15 oocytes are inseminated by their husband's spermatozoa. The extra oocytes are cryopreserved by vitrification. If the patients became pregnant and have healthy live births, the patients...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Cai LB,Qian XQ,Wang W,Mao YD,Yan ZJ,Liu CZ,Ding W,Huang J,Chai DC,Chian RC,Liu JY

    更新日期:2012-02-01 00:00:00

  • Embryo-maternal signalling: how the embryo starts talking to its mother to accomplish implantation.

    abstract::The process of implantation and trophoblast invasion is currently considered as the most limiting factor for the establishment of pregnancy. Molecular interactions at the embryo-maternal interface during the time of adhesion and subsequent invasion are crucial to the process of embryonic implantation. Both partners, t...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Herrler A,von Rango U,Beier HM

    更新日期:2003-03-01 00:00:00

  • A prospective randomized controlled trial of Wallace and Rocket embryo transfer catheters.

    abstract::The aim of this study was to compare the efficacy of two embryo transfer catheters: Wallace and Rocket Embryon in an IVF programme of a tertiary referral university centre. A total of 308 patients undergoing embryo transfer were prospectively randomized to either a transfer with the Wallace catheter or a transfer with...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: El-Shawarby SA,Ravhon A,Skull J,Ellenbogen A,Trew G,Lavery S

    更新日期:2008-10-01 00:00:00

  • Forming a family with sperm donation: a survey of 244 non-biological parents.

    abstract::There has been little research on the views and experiences of non-biological parents of sperm donor children. This paper reports the results of a survey of non-biological mothers and fathers. An online survey was designed and conducted by the Donor Sibling Registry, a US-based non-profit organization that supports th...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Frith L,Sawyer N,Kramer W

    更新日期:2012-06-01 00:00:00

  • Role of embryo quality in predicting early pregnancy loss following assisted reproductive technology.

    abstract::Early pregnancy loss (EPL) significantly reduces the initial success rate of assisted reproduction treatments and increases the psychological burden on the patient. The aim of the current study was to investigate the association between embryo quality and EPL in IVF. A retrospective study of 1471 women undergoing IVF ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Hourvitz A,Lerner-Geva L,Elizur SE,Baum M,Levron J,David B,Meirow D,Yaron R,Dor J

    更新日期:2006-10-01 00:00:00

  • GnRH antagonists and endometrial receptivity in oocyte recipients: a prospective randomized trial.

    abstract::The effect that gonadotrophin-releasing hormone (GnRH) antagonists exert on endometrial receptivity has not yet been elucidated. GnRH antagonists might directly affect oocytes, the embryo and/or the endometrium. The aim of this study was to investigate the direct effect of GnRH antagonists on the endometrium in oocyte...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Prapas N,Tavaniotou A,Panagiotidis Y,Prapa S,Kasapi E,Goudakou M,Papatheodorou A,Prapas Y

    更新日期:2009-02-01 00:00:00

  • Results of assisted reproduction techniques in Latin America.

    abstract::The fact that today it is possible to write a comprehensive report on assisted reproduction technologies in Latin America is the result of a serious and systematic effort, accomplished by more than 80 centres, from Mexico to Chile. Over the past 10 years, these centres representing the vast majority of assisted reprod...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Zegers-Hochschild F,Mackenna A,Fernández E,Sepúlveda MS

    更新日期:2001-01-01 00:00:00

  • Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes.

    abstract::The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Lin YH,Hwang JL,Seow KM,Huang LW,Hsieh BC,Chen HJ,Tzeng CR,Bai CH

    更新日期:2011-07-01 00:00:00

  • Cardiovascular autonomic dysfunction in women with polycystic ovary syndrome: a systematic review and meta-analysis.

    abstract::Recent studies reveal that polycystic ovary syndrome (PCOS) might be associated with cardiovascular autonomic dysfunction, but with inconsistent results. The aim of this meta-analysis was to study whether women with PCOS have cardiovascular autonomic dysfunction. PubMed, Web of Science, Cochrane Library and SCOPUS wer...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,meta分析,评审


    authors: Gui J,Wang RH

    更新日期:2017-07-01 00:00:00

  • Relationship between embryo quality and aneuploidies.

    abstract::Many high-grade embryos selected for transfer according to their morphological evaluation were detected to have chromosomal abnormalities after aneuploidy screening for infertility by preimplantation genetic diagnosis (PGD). The aim of this study was to detect if there is any correlation between embryo quality and gen...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Baltaci V,Satiroglu H,Kabukçu C,Unsal E,Aydinuraz B,Uner O,Aktas Y,Cetinkaya E,Turhan F,Aktan A

    更新日期:2006-01-01 00:00:00

  • Invited commentary on 'Robert G Edwards and the Roman Catholic Church'.

    abstract::The paper 'Robert G. Edwards and the Roman Catholic Church' by Benagiano and colleagues in this issue of Reproductive BioMedicine Online is a very important and timely article published by well-informed authors on the occasion of the long-awaited award of the Nobel Prize to a well-deserved scientist, Bob Edwards. I fo...

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Serour G

    更新日期:2011-06-01 00:00:00