A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage.


:Artificial oocyte activation (AOA) has been previously suggested as a means to overcome the problem of total fertilization failure, which affects about 1-3% of the intracytoplasmic sperm injection (ICSI) cycles. A preliminary study on the application of chemical AOA was conducted using A23187 Ca(2+) ionophore to improve embryonic development in four women with a history of complete fertilization arrest and inability to transit to cleavage stage during previous ICSI trials. Data indicated that activated oocytes resulted in better fertilization, embryonic development and clinical pregnancy in one of the four couples. Therefore, ICSI combined with AOA using Ca(2+) ionophore may be useful in selected patients with cleavage failure, and may help the zygotes to reach more advanced developmental stages.


Reprod Biomed Online


Darwish E,Magdi Y




Has Abstract


2015-12-01 00:00:00














  • The role of in-vivo and in-vitro maturation time on ooplasmic dysmaturity.

    abstract::This study investigates whether the timing of in-vivo and in-vitro maturation influences ooplasmic dysmaturity. This is a retrospective comparison of intracytoplasmic sperm injection (ICSI) cycles (index cycles) complicated by complete fertilization failure (CFF) to cycles with successful fertilization in the same pat...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Pereira N,Neri QV,Lekovich JP,Palermo GD,Rosenwaks Z

    更新日期:2016-04-01 00:00:00

  • Chemiluminescence technique for measuring reactive oxygen species.

    abstract::Accurate assessment of reactive oxygen species (ROS) concentrations may help in the diagnosis of infertility. The chemiluminescence technique, which uses a luminometer to measure ROS, is a common method of assessment. A better understanding of the chemiluminescence technique will allow its proper application in reprod...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Agarwal A,Allamaneni SS,Said TM

    更新日期:2004-10-01 00:00:00

  • Vitrification of human mature oocytes in clinical practice.

    abstract::Oocyte cryopreservation has long been the focus of unsuccessful efforts to perfect its clinical application. Lately, vitrification has been shown to provide high degrees of success by different research groups. This review attempts to address the efficiency of oocyte vitrification in clinical practice through an analy...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Cobo A,Vajta G,Remohí J

    更新日期:2009-01-01 00:00:00

  • Annexin A5 haplotype M2 is not a risk factor for recurrent spontaneous abortion in Northern Europe: is there sufficient evidence?

    abstract::The M2 haplotype of the annexin A5 gene is a well-recognized predisposition factor for recurrent spontaneous abortion (RSA). A recent publication by Nagirnaja et al. (2015) in PLoS One discusses the risk role of the M2 haplotype for RSA in cases compared with controls of North European extraction and arrives at a nega...

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Markoff A,Kurahashi H,Grandone E,Bogdanova N

    更新日期:2016-05-01 00:00:00

  • Does the interval between the last GnRH antagonist dose and the GnRH agonist trigger affect oocyte recovery and maturation rates?

    abstract:RESEARCH QUESTION:Does the time interval between the last gonadotrophin-releasing hormone (GnRH) antagonist dose and the GnRH agonist trigger affect the efficacy of the trigger in IVF treatments? DESIGN:This retrospective cohort study involved 53 normogonadotrophic patients undergoing GnRH antagonist-based IVF cycles,...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Horowitz E,Mizrachi Y,Farhi J,Raziel A,Weissman A

    更新日期:2020-11-01 00:00:00

  • Influence of temperature and sperm preparation on the quality of spermatozoa.

    abstract::This study investigated the effects of long-term (24h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n=41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influe...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Thijssen A,Klerkx E,Huyser C,Bosmans E,Campo R,Ombelet W

    更新日期:2014-04-01 00:00:00

  • The luteal phase after GnRH-agonist triggering of ovulation: present and future perspectives.

    abstract::In stimulated IVF/intracytoplasmic sperm injection cycles, the luteal phase is disrupted, necessitating luteal-phase supplementation. The most plausible reason behind this is the ovarian multifollicular development obtained after ovarian stimulation, resulting in supraphysiological steroid concentrations and consecuti...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Humaidan P,Papanikolaou EG,Kyrou D,Alsbjerg B,Polyzos NP,Devroey P,Fatemi HM

    更新日期:2012-02-01 00:00:00

  • Does slow embryo development predict a high aneuploidy rate on trophectoderm biopsy?

    abstract::The aneuploidy rates in expanded blastocysts biopsied on days 5 and 6 development were assessed in women undergoing IVF followed by array comparative genomic hybridization. This study included 1171 expanded blastocysts from 465 patients. Among the 465 patients, 215 and 141 underwent embryo biopsy on day 5 and day 6 (4...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Piccolomini MM,Nicolielo M,Bonetti TC,Motta EL,Serafini PC,Alegretti JR

    更新日期:2016-09-01 00:00:00

  • Younger poor ovarian response women achieved better pregnancy results in the first three IVF cycles.

    abstract::This retrospective cohort study observed the live birth rates as well as cumulative live birth rates in women with poor ovarian response (POR) undergoing IVF-embryo transfer treatment, stratified for age and cycle number. Four hundred and one patients with POR diagnosed according to the Bologna criteria were enrolled ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Yang Y,Sun X,Cui L,Sheng Y,Tang R,Wei D,Qin Y,Li W,Chen ZJ

    更新日期:2016-05-01 00:00:00

  • Prospectively randomized controlled trial of PGS in IVF/ICSI patients with poor implantation.

    abstract::This randomized, controlled trial verifies whether patients with recurrent failed implantation benefit from preimplantation genetic diagnosis for aneuploidy, as compared with conventional assisted reproduction treatment procedures. Two hundred patients with recurrent failed implantation were randomized into two groups...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Blockeel C,Schutyser V,De Vos A,Verpoest W,De Vos M,Staessen C,Haentjens P,Van der Elst J,Devroey P

    更新日期:2008-12-01 00:00:00

  • Effectiveness of ovarian age as the background risk for aneuploidy screening in an unselected pregnant population.

    abstract::The aim of this study was to assess the performance of first-trimester combined screening when replacing the chronological maternal age by Anti-Müllerian hormone (AMH) and antral follicle count (AFC)-derived ovarian ages, as the background risk in trisomy risk estimation. A total of 639 pregnant women who completed fi...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Grande M,Sabrià J,Borobio V,Mercadé I,Stergiotou I,Masoller N,Borrell A

    更新日期:2016-10-01 00:00:00

  • Preconception serum lipids and lipophilic micronutrient levels are associated with live birth rates after IVF.

    abstract:RESEARCH QUESTION:Is a mixture of preconception serum lipids and lipophilic micronutrients associated with clinical pregnancy and live births? DESIGN:In this prospective cohort study, blood serum was collected on the day of oocyte retrieval for 180 women undergoing IVF at an academic reproductive health centre. Concen...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Jamro EL,Bloom MS,Browne RW,Kim K,Greenwood EA,Fujimoto VY

    更新日期:2019-10-01 00:00:00

  • Techniques of laparoscopic myomectomy.

    abstract::Myomectomy is one of the commonest gynaecological operations. Laparoscopic myomectomy has emerged over the last two decades as a possible alternative to the traditional laparotomy. Most studies have revealed that the laparoscopic procedure is at least as safe as the open procedure as to the rate of complications and m...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Mattei A,Cioni R,Bargelli G,Scarselli G

    更新日期:2011-07-01 00:00:00

  • Monochorionic triplets after single embryo transfer.

    abstract::A 40-year-old patient underwent intracytoplasmic sperm injection and assisted hatching, and a single embryo was transferred. Ultrasonography demonstrated a single gestational sac containing monochorionic tri-amniotic pregnancy. Several factors that have been implicated in the aetiology of monozygotic triple pregnancie...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Rísquez F,Gil M,D'Ommar G,Poo M,Sosa A,Piras M

    更新日期:2004-10-01 00:00:00

  • Symposium: innovative techniques in human embryo viability assessment. Soluble human leukocyte antigen-G and pregnancy success.

    abstract::Non-invasive methods of assessing embryo quality are critical for pregnancy success following IVF or intracytoplasmic sperm injection (ICSI). The addition of new non-invasive morphological and biochemical analyses may further improve pregnancy success, allowing the transfer of a single embryo, thereby reducing the ris...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Warner CM,Lampton PW,Newmark JA,Cohen J

    更新日期:2008-10-01 00:00:00

  • Fibroblast growth factor 10 in human ovaries.

    abstract::The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Oron G,Fisch B,Zhang XY,Gabbay-Benziv R,Kessler-Icekson G,Krissi H,Ben-Haroush A,Ao A,Abir R

    更新日期:2012-10-01 00:00:00

  • Effect of incubation with different concentrations and durations of FSH for in-vitro maturation of murine oocytes.

    abstract::The purpose of the study was to evaluate whether a lower concentration of FSH or 2-h incubation with FSH would improve the outcome of in-vitro maturation of oocytes. The immature oocytes were obtained from FVB mice, and were allocated to four groups and incubated in the maturation media for 24 h. The maturation media ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Lin YH,Hwang JL,Seow KM,Huang LW,Hsieh BC,Chen HJ,Tzeng CR,Bai CH

    更新日期:2011-07-01 00:00:00

  • Circulatory metastin/kisspeptin-1 in polycystic ovary syndrome: a systematic review and meta-analysis with diagnostic test accuracy.

    abstract:RESEARCH QUESTION:A close association between Kisspeptin-1 (KISS-1) and reproductive physiology has been reported, but the results on circulatory KISS-1 are ambiguous in patients with polycystic ovary syndrome (PCOS). A systematic review and meta-analysis were conducted to evaluate the association between KISS-1 and PC...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,meta分析


    authors: Varikasuvu SR,Prasad VS,Vamshika VC,Satyanarayana MV,Panga JR

    更新日期:2019-10-01 00:00:00

  • Sex selection, public policy and the HFEA's role in political decision making - response to Edgar Dahl's 'The presumption in favour of liberty'.

    abstract::The Human Fertilisation and Embryology Authority's report on sex selection is criticized because its recommendation to prohibit all techniques for social sexing is based on unsupported allegations. As a body with a scientific status, the Authority should allow the application of some methods of sex selection for non-m...

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Pennings G

    更新日期:2004-03-01 00:00:00

  • GnRH agonist and GnRH antagonist protocols in ovarian stimulation: differential regulation pathway of aromatase expression in human granulosa cells.

    abstract::Gonadotrophin-releasing hormone (GnRH) agonists and antagonists have been widely used to prevent premature LH surge during ovarian stimulation. However, studies have shown a significantly lower serum oestradiol concentration on the day of human chorionic gonadotrophin administration for cycles using GnRH antagonist. T...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Khalaf M,Mittre H,Levallet J,Hanoux V,Denoual C,Herlicoviez M,Bonnamy PJ,Benhaim A

    更新日期:2010-07-01 00:00:00

  • Preimplantation genetic testing for aneuploidy in severe male factor infertility.

    abstract:RESEARCH QUESTION:Does the use of preimplantation genetic testing for aneuploidies (PGT-A) improve outcomes in couples with severe male factor infertility (SMFI)? DESIGN:This retrospective cohort study included SMFI cases that underwent blastocyst transfer with/without PGT-A. Inclusion criteria were SMFI (azoospermia ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Asoglu MR,Celik C,Serefoglu EC,Findikli N,Bahceci M

    更新日期:2020-10-01 00:00:00

  • Is the occurrence of the empty follicle syndrome a predictor that a subsequent stimulated cycle will be an unfavourable one?

    abstract::Several hypotheses have been advanced to explain empty follicle syndrome (EFS) but it remains a controversial topic. This paper reports experience with three IVF cycles in which no oocytes were collected. In all cases, an additional IVF cycle was performed. The ovarian stimulation protocol, ultrasound and hormonal sur...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Lorusso F,Depalo R,Tsadilas S,Caradonna F,Di Gilio A,Capotorto MT,Vacca M,Nappi L,Selvaggi L

    更新日期:2005-05-01 00:00:00

  • Extrapituitary effects of GnRH antagonists in assisted reproduction: a review.

    abstract::GnRH antagonists are used to prevent premature LH surges in assisted reproduction. The pregnancy rates obtained in several studies have been similar to those obtained in the long protocol with gonadotrophin-releasing hormone (GnRH) agonists. However, lower oestrogen serum concentrations have been observed and fewer oo...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Engel JB,Riethmüller-Winzen H,Diedrich K

    更新日期:2005-02-01 00:00:00

  • Ethanol sclerotherapy: a treatment option for ovarian endometriomas before ovarian stimulation.

    abstract::Several surgical treatment modalities have been described in cases of isolated or multiple ovarian endometriotic cysts. The aim of this preliminary study was to investigate and test the efficacy of ethanol sclerotherapy (EST) for recurrent endometriotic cysts, before ovarian stimulation in infertile patients with an a...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Yazbeck C,Madelenat P,Ayel JP,Jacquesson L,Bontoux LM,Solal P,Hazout A

    更新日期:2009-07-01 00:00:00

  • Association between early embryo morphokinetics plus cumulus cell gene expression and assisted reproduction outcomes in polycystic ovary syndrome women.

    abstract:RESEARCH QUESTION:Can a combination of time-lapse morphokinetic parameters and cumulus cell gene expression in polycystic ovary syndrome (PCOS) women be used to predict assisted reproductive treatment outcome? DESIGN:A total of 547 embryos from 100 intracytoplasmic sperm injection (ICSI) cycles were evaluated. Fifty w...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Tabibnejad N,Sheikhha MH,Ghasemi N,Fesahat F,Soleimani M,Aflatoonian A

    更新日期:2019-02-01 00:00:00

  • In-vitro model systems for the study of human embryo-endometrium interactions.

    abstract::Implantation requires highly orchestrated interactions between the developing embryo and maternal endometrium. The association between abnormal implantation and reproductive failure is evident, both in normal pregnancy and in assisted reproduction patients. Failure of implantation is the pregnancy rate-limiting step i...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Weimar CH,Post Uiterweer ED,Teklenburg G,Heijnen CJ,Macklon NS

    更新日期:2013-11-01 00:00:00

  • Outcome of early versus late multifetal pregnancy reduction.

    abstract::Multifetal pregnancy reduction (MPR) is an accepted method of reducing complications of triplet pregnancies and higher-order multifetal pregnancies. Eighty-three pregnancies that underwent early (68 weeks) transvaginal MPR were compared with 125 pregnancies that underwent late (11-14 weeks) transabdominal MPR. Rates o...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Haas J,Barzilay E,Hourvitz A,Dor J,Lipitz S,Yinon Y,Shlomi M,Shulman A

    更新日期:2016-11-01 00:00:00

  • The emperor still looks naked.

    abstract::Preimplantation genetic screening for aneuploidy continues to excite disagreement, discussion and confusion in the field of reproductive medicine. This commentary provides a contribution to the ongoing debate. ...

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Braude P

    更新日期:2018-08-01 00:00:00

  • The Bertarelli Foundation's actions in the field of multiple gestations.

    abstract::The Bertarelli Foundation aims to promote understanding of infertility. Two international conferences in 1999 and 2002 have highlighted the importance of multiple gestations, which have been recognized as a major problem associated with assisted reproductive technologies and ovulation induction therapies. For this rea...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Germond M

    更新日期:2007-01-01 00:00:00

  • Adverse pregnancy and neo-natal outcomes after assisted reproductive treatment in patients with pelvic endometriosis: a case-control study.

    abstract::To assess the impact of endometriosis on obstetric outcomes and to determine whether the severity, location and surgical treatment of the disease before the pregnancy had an impact on the prevalence of these disorders, a monocentric, case-control study was performed. In total, 113 pregnancies obtained by assisted repr...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Jacques M,Freour T,Barriere P,Ploteau S

    更新日期:2016-06-01 00:00:00