A preliminary report of successful cleavage after calcium ionophore activation at ICSI in cases with previous arrest at the pronuclear stage.


:Artificial oocyte activation (AOA) has been previously suggested as a means to overcome the problem of total fertilization failure, which affects about 1-3% of the intracytoplasmic sperm injection (ICSI) cycles. A preliminary study on the application of chemical AOA was conducted using A23187 Ca(2+) ionophore to improve embryonic development in four women with a history of complete fertilization arrest and inability to transit to cleavage stage during previous ICSI trials. Data indicated that activated oocytes resulted in better fertilization, embryonic development and clinical pregnancy in one of the four couples. Therefore, ICSI combined with AOA using Ca(2+) ionophore may be useful in selected patients with cleavage failure, and may help the zygotes to reach more advanced developmental stages.


Reprod Biomed Online


Darwish E,Magdi Y




Has Abstract


2015-12-01 00:00:00














  • Pregnancy and live birth following the transfer of vitrified-warmed blastocysts derived from zona- and corona-cell-free oocytes.

    abstract::This study reports two clinical pregnancies and one live birth following the transfer of vitrified blastocysts developed from oocytes with neither zona pellucida nor corona cells. Two zona-free oocytes obtained from two patients of advanced maternal age undergoing minimal stimulation were normally fertilized after int...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Shu Y,Peng W,Zhang J

    更新日期:2010-10-01 00:00:00

  • Removal of cervical mucus: effect on pregnancy rates in IVF/ICSI.

    abstract::Cervical mucus may cover the embryo transfer catheter during passage of the cervical canal, interfering with the correct placement of the embryo(s) into the uterine cavity. The effect of removal of cervical mucus prior to embryo transfer in IVF/ intracytoplasmic sperm injection (ICSI) on live birth rate was studied. T...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Visschers BA,Bots RS,Peeters MF,Mol BW,van Dessel HJ

    更新日期:2007-09-01 00:00:00

  • Optimal follicle and oocyte numbers for cryopreservation of all embryos in IVF cycles at risk of OHSS.

    abstract::Ovarian hyperstimulation syndrome (OHSS) is a rare but potentially fatal complication of IVF treatment. The risk of OHSS increases with increasing numbers of follicles aspirated and oocytes retrieved, but there is little evidence to support whether threshold values of either can be used to correctly predict OHSS. Sinc...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Verwoerd GR,Mathews T,Brinsden PR

    更新日期:2008-09-01 00:00:00

  • The correlation between morphology and implantation of euploid human blastocysts.

    abstract:RESEARCH QUESTION:Does the composite morphology score or a particular developmental component (expansion stage, inner cell mass [ICM] or trophectoderm [TE]) of euploid blastocysts undergoing single frozen embryo transfer (FET) impact ongoing pregnancy/live birth (OP/LB) rates? DESIGN:Retrospective cohort study includi...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Nazem TG,Sekhon L,Lee JA,Overbey J,Pan S,Duke M,Briton-Jones C,Whitehouse M,Copperman AB,Stein DE

    更新日期:2019-02-01 00:00:00

  • Pathophysiology proposed as the basis for modern management of the ovarian endometrioma.

    abstract::Present management of the ovarian endometrioma focuses on the size of the cyst and dictates that surgery should not be performed unless this exceeds 3 cm, which neglects the complex pathology of this condition. Studies of ovaries with the endometrioma in situ show progressive smooth muscle cell metaplasia and fibrosis...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Brosens I,Gordts S,Puttemans P,Benagiano G

    更新日期:2014-02-01 00:00:00

  • Adverse pregnancy and neo-natal outcomes after assisted reproductive treatment in patients with pelvic endometriosis: a case-control study.

    abstract::To assess the impact of endometriosis on obstetric outcomes and to determine whether the severity, location and surgical treatment of the disease before the pregnancy had an impact on the prevalence of these disorders, a monocentric, case-control study was performed. In total, 113 pregnancies obtained by assisted repr...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Jacques M,Freour T,Barriere P,Ploteau S

    更新日期:2016-06-01 00:00:00

  • Follow-up in the field of reproductive medicine: an ethical exploration.

    abstract:RESEARCH QUESTION:What ethical implications, issues and concerns play a role in conducting follow-up studies of children born after assisted reproductive technologies (ART)? DESIGN:Literature study and relevant experiences of academic medical centres in Brussels, Belgium, and Maastricht, the Netherlands were used to i...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Jans V,Dondorp W,Bonduelle M,de Die C,Mertes H,Pennings G,de Wert G

    更新日期:2020-12-01 00:00:00

  • Influence of temperature and sperm preparation on the quality of spermatozoa.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Thijssen A,Klerkx E,Huyser C,Bosmans E,Campo R,Ombelet W

    更新日期:2014-04-01 00:00:00

  • IVF versus ICSI for the fertilization of in-vitro matured human oocytes.

    abstract::Traditional dogma suggests that intracytoplasmic sperm injection (ICSI) should be performed to ensure successful oocyte fertilization in an in-vitro maturation (IVM) cycle. This study postulated that there would be no difference in the fertilization rate when ICSI was compared with IVF. This hypothesis was tested in a...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Walls M,Junk S,Ryan JP,Hart R

    更新日期:2012-12-01 00:00:00

  • Invited commentary on 'Robert G Edwards and the Roman Catholic Church'.

    abstract::The paper 'Robert G. Edwards and the Roman Catholic Church' by Benagiano and colleagues in this issue of Reproductive BioMedicine Online is a very important and timely article published by well-informed authors on the occasion of the long-awaited award of the Nobel Prize to a well-deserved scientist, Bob Edwards. I fo...

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Serour G

    更新日期:2011-06-01 00:00:00

  • Effect of human telomerase reverse transcriptase transfection on differentiation in BeWo choriocarcinoma cells.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Neelima PS,Rao Rekha M,Rama S,Rao AJ

    更新日期:2009-06-01 00:00:00

  • Construction and fertilization of reconstituted human oocytes.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Takeuchi T,Neri QV,Palermo GD

    更新日期:2005-09-01 00:00:00

  • Predicting pregnancy rate following multiple embryo transfers using algorithms developed through static image analysis.

    abstract::Single-embryo image assessment involves a high degree of inaccuracy because of the imprecise labelling of the transferred embryo images. In this study, we considered the entire transfer cycle to predict the implantation potential of embryos, and propose a novel algorithm based on a combination of local binary pattern ...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Tian Y,Wang W,Yin Y,Wang W,Duan F,Zhao S

    更新日期:2017-05-01 00:00:00

  • Analysis of 122 triplet and one quadruplet pregnancies after single embryo transfer in Japan.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Yamashita S,Ikemoto Y,Ochiai A,Yamada S,Kato K,Ohno M,Segawa T,Nakaoka Y,Toya M,Kawachiya S,Sato Y,Takahashi T,Takeuchi S,Nomiyama M,Tabata C,Fujiwara T,Okamoto S,Kawamura T,Kawagoe J,Yamada M,Sato Y,Marumo G,

    更新日期:2020-03-01 00:00:00

  • Coasting, embryo development and outcomes of blastocyst transfer: a case-control study.

    abstract::This study compared the effect on blastocyst development and clinical outcome of coasting in women at increased risk of moderate-severe ovarian hyperstimulation syndrome (OHSS; n=389) with a control group matched for age and basal FSH that did not undergo coasting (n=386) in IVF/intracytoplasmic sperm injection (ICSI)...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Talebi Chahvar S,Zosmer A,Caragia A,Balestrini S,Sabatini L,Tranquilli AL,Al-Shawaf T

    更新日期:2014-08-01 00:00:00

  • Freeze-thaw programmes rescue the implantation of day 6 blastocysts.

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    journal_title:Reproductive biomedicine online

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    authors: Murata Y,Oku H,Morimoto Y,Tokuda M,Murata T,Sugihara K,Nagata F,Nakaoka Y,Fukuda A

    更新日期:2005-10-01 00:00:00

  • Spindle examination in unfertilized eggs using the polarization microscope can assist rescue ICSI.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Moon JH,Son WY,Henderson S,Mahfoudh A,Dahan M,Holzer H

    更新日期:2013-03-01 00:00:00

  • Extended embryo culture to increase implantation rate.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Quea G,Romero K,Garcia-Velasco JA

    更新日期:2007-01-01 00:00:00

  • The impact of music therapy on pain and stress reduction during oocyte retrieval - a randomized controlled trial.

    abstract:RESEARCH QUESTION:Does music therapy help in reducing pain and anxiety in women undergoing transvaginal ultrasound-guided oocyte retrieval (TUGOR)? DESIGN:In this randomized controlled open label study, 209 participants were recruited and randomized into three groups (music group, n = 70; headphone group, n = 70; cont...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,随机对照试验


    authors: Cheung CWC,Yee AWW,Chan PS,Saravelos SH,Chung JPW,Cheung LP,Kong GWS,Li TC

    更新日期:2018-08-01 00:00:00

  • Monochorionic triplets after single embryo transfer.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Rísquez F,Gil M,D'Ommar G,Poo M,Sosa A,Piras M

    更新日期:2004-10-01 00:00:00

  • Subsidized in-vitro fertilization treatment and the effect on the number of egg sharers.

    abstract::Egg sharing remains a controversial practice, mainly because of the presumed element of payment. In order to find out to what extent financial considerations motivated the women to share their oocytes, the data on egg sharing in Belgium are analysed. Belgium began providing full reimbursement for six in-vitro fertiliz...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Pennings G,Devroey P

    更新日期:2006-07-01 00:00:00

  • Role of LH in ovarian stimulation: considerations.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Griesinger G,Diedrich K

    更新日期:2006-04-01 00:00:00

  • Effect of human embryonic morphological development on fetal growth parameters: the Rotterdam Periconceptional Cohort (Predict Study).

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Parisi F,Rousian M,Koning AHJ,Willemsen SP,Steegers EAP,Steegers-Theunissen RPM

    更新日期:2019-04-01 00:00:00

  • Effectiveness of ovarian age as the background risk for aneuploidy screening in an unselected pregnant population.

    abstract::The aim of this study was to assess the performance of first-trimester combined screening when replacing the chronological maternal age by Anti-Müllerian hormone (AMH) and antral follicle count (AFC)-derived ovarian ages, as the background risk in trisomy risk estimation. A total of 639 pregnant women who completed fi...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Grande M,Sabrià J,Borobio V,Mercadé I,Stergiotou I,Masoller N,Borrell A

    更新日期:2016-10-01 00:00:00

  • Advanced oocyte cryopreservation will not undermine the practice of ethical egg sharing.

    abstract::The fear that the ethics and practice of egg sharing will be undermined by the growing success of oocyte cryopreservation is not based on published evidence. Separate contracts and directed counselling of donors and recipients as required by law in the UK provide protection against potential pitfalls with egg sharing....

    journal_title:Reproductive biomedicine online

    pub_type: 评论,杂志文章


    authors: Ahuja KK,Simons EG

    更新日期:2006-03-01 00:00:00

  • Failure mode and effects analysis of witnessing protocols for ensuring traceability during IVF.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Rienzi L,Bariani F,Dalla Zorza M,Romano S,Scarica C,Maggiulli R,Nanni Costa A,Ubaldi FM

    更新日期:2015-10-01 00:00:00

  • Mutations in HOXA11 are not responsible for Müllerian duct anomalies in Chinese patients.

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    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Chen X,Li G,Qin Y,Cui Y,You L,Chen ZJ

    更新日期:2014-06-01 00:00:00

  • Experience with transvaginal hydrolaparoscopy for reconstructive tubo-ovarian surgery.

    abstract::The transvaginal approach to tubo-ovarian surgery allows easy and direct access to the tubo-ovarian structures and the fossa ovarica without additional manipulation. In the absence of a panoramic view, the proximity of the tubo-ovarian structures allows operative procedures for treatment of superficial and cystic ovar...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,评审


    authors: Gordts S,Campo R,Brosens I

    更新日期:2002-01-01 00:00:00

  • The biological basis for defining bi-parental or tri-parental origin of offspring from cytoplasmic and spindle transfer.

    abstract::The bi-parental genetic state is not a given after assisted reproduction. This is based on a biological definition of parentage that concerns generational inheritance of genetic material. Often three or more individuals may participate in artificial reproduction. Only cytoplasmic and spindle transfer can result in the...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章


    authors: Cohen J,Alikani M

    更新日期:2013-06-01 00:00:00

  • Randomized, controlled, open-label, non-inferiority study of the CONSORT algorithm for individualized dosing of follitropin alfa.

    abstract::In this randomized, controlled, open-label, phase IV study, ovarian response after a follitropin alfa starting dose determined by the CONSORT calculator was compared with a standard dose (150 IU). Normo-ovulatory women (aged 18-34 years) eligible for assisted reproductive techniques were recruited (23 centres: nine Eu...

    journal_title:Reproductive biomedicine online

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Olivennes F,Trew G,Borini A,Broekmans F,Arriagada P,Warne DW,Howles CM

    更新日期:2015-03-01 00:00:00