NOD2 and ATG16L1 polymorphisms affect monocyte responses in Crohn's disease.

Abstract:

AIM:To assess whether polymorphisms in NOD2 and ATG16L1 affect cytokine responses and mycobacterium avium subspecies paratuberculosis (MAP) survival in monocytes from Crohn's disease (CD) patients. METHODS:Monocytes were isolated from peripheral blood of CD patients of known genotype for common single nucleotide polymorphisms of NOD2 and ATG16L1. Monocytes were challenged with MAP and bacterial persistence assessed at subsequent time-points. Cytokine responses were assayed using a Milliplex multi-analyte profiling assay for 13 cytokines. RESULTS:Monocytes heterozygous for a NOD2 polymorphism (R702W, P268S, or 1007fs) were more permissive for growth of MAP (P = 0.045) than those without. There was no effect of NOD2 genotype on subsequent cytokine expression. The T300A polymorphism of ATG16L1 did not affect growth of MAP in our model (P = 0.175), but did increase expression of cytokines interleukin (IL)-10 (P = 0.047) and IL-6 (P = 0.019). CONCLUSION:CD-associated polymorphisms affected the elimination of MAP from ex vivo monocytes (NOD2), or expression of certain cytokines (ATG16L1), implying independent but contributory roles in the pathogenesis of CD.

journal_name

World J Gastroenterol

authors

Glubb DM,Gearry RB,Barclay ML,Roberts RL,Pearson J,Keenan JI,McKenzie J,Bentley RW

doi

10.3748/wjg.v17.i23.2829

subject

Has Abstract

pub_date

2011-06-21 00:00:00

pages

2829-37

issue

23

eissn

1007-9327

issn

2219-2840

journal_volume

17

pub_type

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